[Repeated drenching of calves - -Ultra-sonographic findings of the -reticulum and abomasum and -short-term -effects on pH and -D-lactate in rumen fluid and blood]. / Wiederholtes Drenchen von Kälbern ­ Ultraschallbefunde an Haube und Labmagen sowie kurzfristige Auswirkungen auf pH-Wert und D-Laktat in Pansensaft und Blut.

INTRODUCTION: An oesophageal tube feeder was used to administer milk to six clinically healthy calves every 12 hours for a total of three feedings. The calves were seven to nine days of age, and each feeding consisted of a volume of milk that was 6 % of the body weight of the calves. The reticulum and abomasum were scanned ultrasonographically before, during and after drenching. Blood samples for D-lactate determination and venous blood gas analysis were collected at -12, 0 (first drenching), 6, 12 (second drenching), 18, 24 (third drenching), 30 and 36 hours. Ruminal fluid was collected for measurement of pH and D-lactate concentration at -12 and 36 hours. Based on simultaneous ultrasonographic examinations of the reticulum and abomasum, some of the force-fed milk flowed into the reticulum and some directly into the abomasum; both organs contained milk immediately after drenching. Drenching led to ruminal acidosis evidenced by a significant decrease in median pH from 7,0 (6,0 - 7,0) to 4,5 (3,0 - 5,0) and a 93-fold increase in median D-lactate concentration from 0,39 (0,14 - 1,33) to 36,3 (17,9 - 53,3) mmol/l but it did not cause metabolic acidosis. Based on these findings, we concluded that milk administered to calves via an oesophageal tube feeder flows into the reticulum and abomasum simultaneously and three force feedings 12 hours apart cause acute ruminal acidosis, which was not accompanied by metabolic acidosis in the short term.

INTRODUCTION: Une sonde d'alimentation œsophagienne a été utilisée pour administrer par trois fois du lait à six veaux cliniquement sains toutes les 12 heures. Les veaux étaient âgés de sept à neuf jours, et chaque repas comprenait un volume de lait correspondant à 6 % de leur poids corporel. Le réticulum et la caillette ont été examinés par ultrasons avant, pendant et après le drenchage. Des échantillons de sang pour la détermination du D-lactate et pour l'analyse des gaz du sang veineux ont été prélevés à ­12, 0 (premier drenchage), 6, 12 (deuxième drenchage), 18, 24 (troisième drenchage), 30 et 36 heures. Le jus de panse a été prélevé pour mesurer le pH et la concentration de D-lactate à ­12 et 36 heures. D'après les examens ultrasonographiques simultanés du réticulum et de la caillette, une partie du lait administré s'est écoulée dans le réticulum et une autre directement dans la caillette : les deux organes contenaient du lait immédiatement après le drenchage. Le drenchage a entraîné une acidose ruminale mise en évidence par une diminution significative du pH médian de 7,0 (6,0 ­ 7,0) à 4,5 (3,0 ­ 5,0) et une concentration médiane de D-lactate 93 fois plus élevée de 0,39 (0,14 ­ 1,33) à 36,3 (17,9 ­ 53,3) mmol/l, mais il n'a pas provoqué d'acidose métabolique. Sur la base de ces résultats, nous avons conclu que le lait administré aux veaux par l'intermédiaire d'une sonde d'alimentation œsophagienne s'écoule simultanément dans le réticulum et la caillette et que trois gavages à 12 heures d'intervalle provoquent une acidose ruminale aiguë qui ne s'est pas accompagnée d'une acidose métabolique à court terme.

[Usage of antimicrobials on seven farms of beef producers in Switzerland]. / Einsatz von Antibiotika bei Aufzuchtkälbern für die Grossviehmast bzw. Fresseraufzucht auf sieben Schweizer Betrieben.

INTRODUCTION: It was the objective of this study to evaluate morbidity, mortality, use of veterinary drugs and production output on seven representative Swiss beef fatteners, who bought on a regular basis batches of calves from traders (mean age and weight: 31 day, 75 kg) and practised an all in-all out system. From March 2015 until May 2016, 51 batches with a total of 1307 calves were included. The use of drugs during the first eight weeks following delivery of calves from the trader, casualty rate and carcass performance of 837 bulls. The administration of antimicrobials was calculated per calf as the number of days with effective therapeutic concentrations (daily doses per animal; DD/A). The average weight of the calves at arrival on the fattening unit was 75 kg and the age 31 days. The group size was between 13 to 47 calves (median 22, Q1 = 21, Q2 = 30). In 36 batches (70,6 %), a veterinary entry examination was performed which demonstrated that at admission 20 % of the calves suffered from a relevant disease. The median for the number of DD/A was 16 for the first 56 days on the farm (Q1 = 11, Q3 = 21); 68 % of these DD/A were attributed to metaphylactic medications after delivery. Thereafter, pneumonia (54 %), diarrhea (33 %) and otitis (10 %) were the most frequent indications for subsequent therapeutic interventions. Over all batches, a median of 71 % (Q1 = 47, Q3 = 85) of all DD/A were due to critically important antimicrobials (CIM) and drugs containing several antibiotics including a CIM. The losses due to death or euthanasia averaged 3,7 % of all calves. The median for daily weight gain for the total fattening period (median 354 days; Q1 = 328, Q3 = 379) accounted for 1325 g (Q1 = 1216, Q3 = 1425). The profound variation between the farms in respect to the results of drug use and production output reveals evidence for the strong impact of farm-specific factors (such as pen space per calf, barn climate, vaccination protocol, supervision). Concurrently, the remarkable differences between the results of different batches on one farm depict that there are further important risk factors which are beyond the control of the farmer (in particular season of the year, transport). It is concluded that a systematic minimization of risk factors as part of a continuous veterinary consultation is useful two improve herd health and to reduce the input of drugs in the production of beef.

INTRODUCTION: L'objectif de cette étude était d'évaluer la morbidité, la mortalité, l'utilisation de médicaments vétérinaires et la production de sept engraisseurs suisses représentatifs, qui achetaient régulièrement des lots de veaux à des commerçants (âge et poids moyens : 31 jours, 75 kg). et pratiquant le système «  all in-all out ¼. De mars 2015 à mai 2016, 51 lots avec un total de 1307 veaux ont été inclus. On a étudié l'utilisation de médicaments pendant les huit premières semaines suivant la livraison des veaux, le taux de mortalité et la performance carcasse de 837 taureaux. L'administration d'antimicrobiens par veau a été calculée individuellement en nombre de jours avec des concentrations thérapeutiques efficaces (doses quotidiennes par animal ; JJ/A). Le poids moyen des veaux à l'arrivée sur l'unité d'engraissement était de 75 kg et l'âge de 31 jours. La taille du groupe était comprise entre 13 et 47 veaux (médiane 22, Q1 = 21, Q2 = 30). Dans 36 lots (70,6 %), un examen vétérinaire d'entrée a été réalisé qui a démontré qu'à l'admission 20 % des veaux souffraient d'une maladie nécessitant un traitement. La médiane du nombre de DD/A était de 16 pour les 56 premiers jours à la ferme (Q1 = 11, Q3 = 21) ; 68 % de ces DD/A ont été constitués par des médicaments métaphylactiques après la livraison. Par la suite, la pneumonie (54 %), la diarrhée (33 %) et l'otite (10 %) étaient les indications les plus fréquentes des interventions thérapeutiques ultérieures. Sur l'ensemble des lots, une médiane de 71 % (Q1 = 47, Q3 = 85) de tous les DD/A étaient dus à des antimicrobiens d'importance critique (CIM) et à des médicaments contenant plusieurs antibiotiques, dont un CIM. Les pertes dues à la mort ou à l'euthanasie représentaient en moyenne 3,7 % de tous les veaux. La médiane du gain de poids quotidien pour la période d'engraissement totale (médiane 354 jours ; Q1 = 328, Q3 = 379) représentait 1325 g (Q1 = 1216, Q3 = 1425). La variation importante entre les exploitations en ce qui concerne les résultats de l'utilisation de médicaments et la production est la preuve du fort impact des facteurs spécifiques à l'exploitation (tels que l'espace en enclos par veau, le climat de l'étable, le protocole de vaccination, la supervision). Parallèlement, les différences remarquables entre les résultats de différents lots sur une exploitation montrent qu'il existe d'autres facteurs de risque importants qui échappent au contrôle de l'agriculteur (en particulier la saison, le transport). Il est conclu qu'une minimisation systématique des facteurs de risque dans le cadre d'une consultation vétérinaire continue est utile pour améliorer la santé du troupeau et réduire l'apport de médicaments dans la production de viande bovine.

Short communication: Variance and autocorrelation of deviations in daily milk yield are related with clinical mastitis in dairy cows.

Daily milk production, and fluctuations therein, can provide information on health and resilience of dairy cows. We studied variance and autocorrelation of deviations in daily milk yield in relation to the occurrence of clinical mastitis (no, early or later in lactation). Individual lactation curves were fitted to 305-d lactations of 414 dairy cows using quantile regression. Log-transformed variance (lnVar) and autocorrelation of the quantile residuals of daily milk yield (predicted - observed) were evaluated for intervals until 30 and until 305 days in milk (DIM). Cows were classified as having no mastitis (n = 249), early mastitis that first occurred before 30 DIM (n = 29); or later mastitis (n = 136). Subsequently, linear models were used to assess effects of mastitis and parity class (primiparous or multiparous) on lnVar and autocorrelations; and logistic regression analyses were performed to predict mastitis from lnVar or autocorrelation and parity. From 10 to 30 DIM, lnVar was greater for cows with early mastitis than for cows with no or late mastitis, and autocorrelation tended to be lower for cows with early mastitis than for cows with no mastitis. The lnVar and autocorrelation from 10 to 30 DIM were not predictive of late mastitis. From 10 to 305 DIM, lnVar was greater and autocorrelation was lower for both cows with early and late mastitis than for cows with no mastitis; and both were predictive of having mastitis in the 305-d lactation. Primiparous cows had lower lnVar than multiparous cows. In cows without mastitis, autocorrelation values were positively correlated with lnVar. Results confirm that increased lnVar is associated with clinical mastitis.

[Laboratory diagnostic examinations as part of «Pathocalf¼ in case of herd problems on Swiss calf rearing and fattening farms]. / Labordiagnostische Untersuchungen im Rahmen von «Pathocalf¼ bei ­Bestandesproblemen auf Schweizer ­Kälberaufzucht und -mastbetrieben.

INTRODUCTION: «PathoCalf¼ represents a project promoted by the Swiss Federal Food Safety and Veterinary Office. It was the aim of this project to use laboratory diagnostic services for farms raising calves (dairy farms, fattening units, beef cow operations) and dealing with a herd health issue in order to obtain an overview about the spectrum of infectious agents and bacterial resistance patterns in Switzerland. From January 2015 to March 2018, the Bovine Health Service and the farm veterinarian straightened out 148 stock problems on 125 farms. For this, samples were collected from 342 animals. In addition, 98 necropsies were performed. The service related to «PathoCalf¼ was utilized most frequently related to stock problems with respiratory disease (40,5%; 60/148), gastrointestinal disease (37,2%, 55/148) and fatalities with unknown causation (8,8%, 13/148). The majority of all investigated animals (71,8%) were younger than 10 weeks of age. In calves suffering from respiratory disease, most frequently Pasteurella (P.) multocida was found (40,6%, 67/165), followed by mycoplasmen (35,8%, 59/165) and Mannheimia (M.) haemolytica (13,3%, 22/165). The proportion of tested P. multocida and M. haemolytica strains being resistant against oxytetracyline was 67,7% (42/62), 21,1% (4/19) for tilmicosine and 11,3% (7/62) for danofloxacine. For calves suffering from gastrointestinal problems, most frequently Rotavirus (57,6%, 19/33), E. coli (47,7%, 74/155) and Campylobacter spp. (27,7%, 43/155) were found. A resistance against tetracycline was evident for 95,0% (19/20) of all isolated E. coli, and for 80,0% (16/20) in respect to sulfonamides and for 57,1% (12/21) against enrofloxacine. For fatalities with unknown causation, a perforated abomasal ulcer was the most important finding (33,3%, 7/21), followed by diarrhea (28,6%, 6/21) and indigestion related to ruminal acidosis (19,1%, 4/21). The results indicate that aetiological investigations facilitate the evaluation of stock problems. The systematic assessment of abiotic risk factors remains, however, indispensable for the factorial diseases most frequently found on farms raising calves.

INTRODUCTION: «PathoCalf¼ est un projet promu par l'Office fédéral suisse de la sécurité alimentaire et des affaires vétérinaires. L'objectif de ce projet était d'utiliser les services de diagnostic de laboratoire pour les exploitations élevant des veaux (fermes laitières, unités d'engraissement, exploitations de vaches à viande) et confrontées à un problème de santé de troupeau afin d'obtenir une vue d'ensemble du spectre des agents infectieux et de la résistance bactérienne en Suisse. De janvier 2015 à mars 2018, le Service Sanitaire Bovin et/ou le vétérinaire d'exploitation ont traité 148 problèmes dans 125 exploitations. Pour cela, des échantillons ont été prélevés sur 342 animaux. De plus, 98 autopsies ont été réalisées. Le service lié à «PathoCalf¼ a été utilisé le plus souvent en raison de problèmes d'exploitation avec une maladie respiratoire (40,5% ; 60/148), une maladie gastro-intestinale (37,2%, 55/148) ou des décès de cause inconnue (8,8%, 13/148). La majorité de tous les animaux étudiés (71,8%) avaient moins de 10 semaines d'âge. Chez les veaux souffrant de maladies respiratoires, c'est le plus souvent Pasteurella (P.) multocida qui a été trouvé (40,6%, 67/165), suivi par les mycoplasmes (35,8%, 59/165) et Mannheimia (M.) haemolytica (13,3%, 22/165). La proportion des souches de P. multocida et de M. haemolytica testées résistantes à l'oxytétracycline était de 67,7% (42/62), de 21,1% (4/19) pour la tilmicosine et de 11,3% (7/62) pour la danofloxacine. Pour les veaux souffrant de troubles gastro-intestinaux, on a trouvé le plus souvent des Rotavirus (57,6%, 19/33), des E. coli (47,7%, 74/155) et des Campylobacter spp. (27,7%, 43/155). Une résistance à la tétracycline était évidente pour 95,0% 50 (19/20) de tous les E. coli isolés, pour 80,0% (16/20) en ce qui concerne les sulfamides et pour 57,1% (12/21) contre l'enrofloxacine. Pour les décès de cause inconnue, un ulcère de la caillette perforé était la cause la plus importante (33,3%, 7/21), suivie de la diarrhée (28,6%, 6/21) et des indigestions liées à une acidose ruminale (19,1%, 4/21). Les résultats indiquent que les investigations étiologiques facilitent l'évaluation des problèmes de d'exploitation. L'évaluation systématique des facteurs de risque abiotiques reste cependant indispensable pour les maladies factorielles les plus fréquemment recensées sur les exploitations élevant des veaux.

[Economic importance of antibiotic sale for private food animal practices in Switzerland - example veal production]. / Ökonomische Bedeutung des Umsatzes mit Antibiotika für private Nutztierpraxen in der Schweiz ­ Beispiel Kälbermast.

INTRODUCTION: The use of antibiotics in Swiss veal production is considered an established method for controlling bacterial infectious diseases. Although the veterinary profession aims to ensure animal welfare, the veterinary business income needs to be ensured at the same time. Against the background of increasing problems with resistant pathogens in human and veterinary medicine, the use of antibiotics should be significantly reduced and used more selectively. The associated economic consequences for food animal practitioners are unknown. The aim of this study was to determine the economic importance of antibiotic sale volume for private food animal practitioners in veal production. An anonymized questionnaire was sent to 120 mixed veterinary practices in Switzerland, which offered services to veal and beef cattle farmers. Questions involved the pharmaceutical sale volume, details on veterinary invoices from three farms with average, below and above average animal health throughout 2017. Twenty-nine complete questionnaires (response rate: 24.2%) and veterinary invoices of 84 farms were returned. The study is not representative, but it allows a rough assessment of the economic framework in Swiss livestock practice. The majority of the total turnover with livestock farms was generated by the sale of antibiotics (54%). Antibiotic sales per animal were higher as expected in farms with a below-average animal health than in farms with an average or above-average animal health. Consulting services turnover contributed only 0.5% to the total sale volume in veal farming. The results document, that antibiotic reduction measurements in veal and beef production will have economic consequences for veterinary livestock practices. In the medium term, the profitable existence of livestock veterinary practice requires a change to cost based consulting services.

INTRODUCTION: En Suisse, dans le cadre de la pratique de l'engraissement des veaux, l'utilisation d'antibiotiques est principalement considérée comme une méthode éprouvée pour lutter contre les maladies infectieuses bactériennes. L'activité vétérinaire vise à prévenir ou minimiser les souffrances chez les animaux. Mais pour autant, les vétérinaires restent des entrepreneurs qui doivent assurer un revenu adéquat de leur pratique. Dans un contexte de problèmes croissants rencontrés avec les agents pathogènes résistants en médecine humaine et vétérinaire, l'utilisation d'antibiotiques devrait être considérablement réduite et ciblée. Les conséquences économiques associées pour la pratique vétérinaire rurale sont peu connues. Le but de la présente étude était de déterminer l'importance économique des ventes d'antibiotiques pour les pratiques vétérinaires rurales privées en utilisant l'exemple de l'engraissement des veaux. À cet effet, un questionnaire anonymisé a été envoyé à 120 cabinets vétérinaires mixtes en Suisse, qui s'occupent entre autres d'exploitations d'engraissement de veaux, de broutards et de taurillons. Les participants ont été interrogés sur le niveau des ventes de médicaments. En outre, nous les avons invités à envoyer des factures vétérinaires concernant trois entreprises d'engraissement avec un niveau de santé animale inférieur, égal et supérieur à la moyenne tout au long de l'année 2017. Vingt-neuf questionnaires complets (taux de réponse: 24,2%) ainsi que des factures vétérinaires concernant au total 84 exploitations d'engraissement de veaux, de broutards ou de taurillons nous ont été retournés. L'étude n'est certes pas représentative, mais elle permet une évaluation approximative du cadre économique des pratiques rurales en Suisse. La majorité du chiffre d'affaires total sur les exploitations d'engraissement de veaux, de broutards ou taurillons a été générée par la vente d'antibiotiques (54%). Il apparait que les ventes d'antibiotiques étaient plus élevées dans les exploitations où la santé animale était inférieure à la moyenne que dans les établissements où elle était supérieure ou égale à la moyenne. Le chiffre d'affaires des prestations de conseil ne représentait que 0,5% du chiffre d'affaires total dans le domaine des veaux. Ces résultats montrent que les mesures visant à réduire les antibiotiques dans les conditions actuelles de production de veau et de boeuf auront probablement des conséquences économiques non négligeables sur la part des exploitations d'engraissement dans les revenus des pratiques vétérinaire rurales. A moyen terme, afin d'assurer la rentabilité d'une pratique rurale, une nouvelle orientation de l'activité vétérinaire sera nécessaire: la mise en place d'un service de conseils payants pour les exploitations d'engraissement, ayant pour but l'établissement de concepts de prévention.

[Interventions and treatments of farm animals by non-veterinarians: quo vadis?] / Eingriffe und Behandlungen von Nutz­tieren durch Nicht-Tierärzte: quo vadis?

INTRODUCTION: The increasing professionalization and the cost pressure on farms combined with a decreasing availability of veterinary expertise procures the demand for treatments and interventions performed by non-veterinarians. A current survey resulting in 56 responses of food animal practitioners showed that a majority delivered pour-on anthelmintics, drugs for oral application and ointments after respective instruction to farmers. Udder injectors, antimicrobials and vaccines were handled more restrictively, but even hormones were committed by more than half of the veterinarians to livestock owners. In respect to interventions, most practitioners supported that farmers perform functional foot trimming, administration of boli, inseminations, dehorning and castrations of calves. By contrast, many veterinarians refused that farmers apply intravenous injections, insert a trocar or treat sole ulcers. In respect to the future development, implications of non-veterinarian interventions on animal welfare, consumer protection and drug safety have to be considered. Indispensable are qualification and skills of the person performing interventions which can be acquired by certificated training courses. Moreover, a basic conception and taking responsibility in particular for application of veterinary drugs is mandatory - in this respect, at present marked deficits exist among many farmers. Based on the current legal situation, several interventions can be delegated to non-veterinarians, but the technical responsibility for the proper implementation is due to the veterinarian. This includes an evaluation of interventions and treatments on a regular basis together with the farmer. On this basis, the assessment of clinical symptoms as well as the initial treatment can be useful and appropriate to ensure a rapid therapy especially in animals suffering from factorial diseases. Having in mind profound differences in respect to the competence of non-veterinarians, general procedures to deliver drugs and to delegate interventions have to be rejected. Advanced diagnostic procedures and the prescription of veterinary drugs remain an exclusive mission of the veterinarian.

INTRODUCTION: La professionnalisation progressive et la pression des coûts sur les exploitations agricoles, combinées à une disponibilité d'expertise vétérinaire décroissante au moins au niveau régional, font que de plus en plus de traitements et d'interventions sur le bétail peuvent être effectués par des non-vétérinaires. Une enquête récente portant sur les réponses de 56 cabinets pour animaux de rente a révélé que la plupart d'entre eux remettent des anthelminthiques pour-on, des médicaments à administrer par voie orale et des onguents aux propriétaires d'animaux. On est plus restrictif avec les injecteurs intra-mammaires, les antibiotiques et les vaccins, mais même des hormones sont remises aux agriculteurs après instruction par plus de la moitié des vétérinaires. En termes d'interventions, la majorité des praticiens soutiennent la réalisation de soins fonctionnels des onglons, l'administration de boli, l'écornage des veaux, la castration et l'insémination par des non-vétérinaires. En revanche, la plupart des praticiens considèrent que les injections intraveineuses, la mise en place d'un trocart et le traitement d'un ulcère de la sole doivent être effectués par des vétérinaires. En ce qui concerne l'évolution future, il est important de prendre en compte les implications pour le bien-être des animaux, la protection des consommateurs et la sécurité en matière de médicaments. Il est essentiel que l'exécutant ait la compétence nécessaire ainsi que la capacité de prouver qu'il possède une expertise. En outre, une compréhension de base du sujet et la prise de responsabilité, notamment en ce qui concerne l'utilisation de médicaments vétérinaires, sont nécessaires. Bien que le vétérinaire puisse déléguer des actes en raison de la situation juridique actuelle, il assume ensuite la responsabilité professionnelle de la qualité des interventions réalisées. En outre la délégation nécessite impérativement une évaluation rétrospective régulière du type et du nombre des traitements ainsi qu'un contrôle du bon usage des médicaments vétérinaires conformément à l'Ordonnance sur les médicaments vétérinaires. Sous cette prémisse, le recueil des commémoratifs dans un cadre clairement défini et conformément aux exigences légales avec un traitement initial par l'agriculteur peut être, dans la pratique, judicieux, en particulier dans les maladies factorielles pour assurer le traitement le plus rapide possible des animaux malades. Compte tenu des différences considérables en termes de compétence professionnelle parmi les non-vétérinaires, des réglementations forfaitaires devraient être rejetées. Les diagnostics plus approfondis et la prescription de médicaments vétérinaires doivent en principe rester une tâche exclusive du vétérinaire.

Extended lactation in high-yielding dairy cows. I. Effects on reproductive measurements.

The objective of this prospective field study was to evaluate the effects of extending the lactation period on various reproductive measurements of high-yielding Holstein cows. On 40 d in milk (DIM), cows were gynecologically examined (transrectal palpation, sonography, vaginoscopy). Cows without signs of clinical endometritis were blocked by parity and were randomly allocated to 1 of 3 experimental groups with a voluntary waiting period (VWP) of 40, 120, and 180 d, respectively (G40, n = 135; G120, n = 141; G180, n = 139). Cows of G120 and G180 were reexamined at the end of the VWP. If natural estrus was detected within 46 d after the end of the VWP, an artificial insemination was performed. If no estrus was detected, the respective cows were synchronized by applying the classical Ovsynch protocol. We found no difference in the proportion of cows in which estrus was detected between 40 to 86 DIM or in the days to first estrus between the 3 groups. Estrus detection in this period was lower in cows with body condition score <3 on 90 DIM compared with body condition score ≥3 (61.5 vs. 76.0%) and in cows with high energy-corrected milk production (ECM) on 92 DIM [58.6 vs. 70.1%, for cows with higher and lower than the median (39.9 kg) ECM, respectively]. The proportion of cows that estrus was detected within 46 d after the VWP was greater in G120 (88.9%) and G180 (90.8%) compared with G40 (70.4%). These effects were more apparent in cows with high ECM. The rate of estrus detection and of becoming pregnant in this period was greater for G120 (hazard ratio = 2.2 and 1.6, respectively) and for G180 (hazard ratio = 2.4 and 1.8) compared with G40. Cows in both groups with extended lactation had greater overall first service conception rates (G120 = 48.9%; G180 = 49.6%) and a lower number of services per pregnant cow (G120 = 1.56 ± 0.1; G180 = 1.51 ± 0.1) compared with G40 (36.6%; 1.77 ± 0.1). We observed no difference in pregnancy loss or in the proportion of cows culled up to 305 d of lactation between the 3 groups. The number of Ovsynch protocols per 1,000,000 kg of ECM was reduced by 75% in G180 and by 74% in G120 compared with G40 (5.9 vs. 7.1 vs. 25.1). In conclusion, extending the lactation of dairy cows can improve main reproductive measurements in high-yielding cows.

Extended lactation in high-yielding dairy cows. II. Effects on milk production, udder health, and body measurements.

The objective of this prospective field study was to evaluate the effects of extending the lactation period of high-yielding dairy cows on milk production, udder health characteristics, and development of body condition. On 40 d in milk (DIM), an examination of the genital tract (transrectal palpation, sonography, vaginoscopy) was performed. Cows without signs of clinical endometritis were blocked by parity and were randomly allocated to 1 of 3 experimental groups with a voluntary waiting period of 40, 120, and 180 d, respectively (G40, n = 135; G120, n = 141; G180, n = 139). Mean daily milk and energy-corrected milk production did not differ between the 3 groups regarding the first 305 d or for the whole lactation (d 1 and up to dry off, culling, or 600 DIM). In late lactation (306 to 600 DIM), G40 had lower average productivity (23.8 kg) compared with G120 (26.5 kg), with G180 showing intermediate values (25.7 kg). The extended lactation groups showed greater persistency, as the rate of decline based on a Wilmink function was lower for G120 (c = -0.063 and -0.045 for milk and energy-corrected milk, respectively) and G180 (c = -0.061 and -0.047) compared with G40 (c = -0.071 and -0.056). We found no difference between the 3 groups regarding the evaluated udder health characteristics (somatic cell count, incidence of mastitis, and days off milk due to mastitis). More cows in G180 (7.9%) were culled due to low productivity compared with G40 (0.7%) and as a tendency compared with G120 (2.8%). Moreover, cows of G180 showed higher median body condition score at the time of dry off compared with cows of both G40 and G120 (3.50 for G180 vs. 3.25 for both G40 and G120). At the time of dry off, G180 cows also had greater backfat thickness (25.0 mm) compared with both G40 (22.2 mm) and G120 cows (21.6 mm). Based on our results, the extension of the voluntary waiting period of high-yielding cows up to 120 d has no adverse effects regarding milk production, involuntary culling, udder health, or BCS gain.

[AntibioticScout.ch: Decision support for the prudent use of antimicrobials: Application in cattle]. / AntibioticScout.ch: Entscheidungshilfe für den umsichtigen Einsatz von antimikrobiellen Wirkstoffen: Anwendung beim Rind.

INTRODUCTION: The administration of antibiotics in livestock has been criticized for many years, in particular because of an inappropriate use and the appearance of antibiotic residues in the environment, which can promote the emergence and spread of resistant bacteria. However, antibiotics are essential for the successful and sustainable control of bacterial pathogens. With the aim of optimizing the use of antibiotics in food animals and minimizing the prevalence of resistant bacteria, AntibioticScout. ch provides a decision aid for the prudent use of antimicrobial drugs. This approach emphasizes the importance of supportive therapy and the hallmarks of preventive concepts. Procedures to improve animal health and animal welfare in accordance with the principles of good veterinary practice are primary and effective tools to reduce the use of antimicrobial drugs. The necessary reduction in the use of antibiotics must, therefore, be accompanied by appropriate management strategies in animal husbandry. In particular, hygiene, animal welfare and biosecurity measures are crucial to ensure an optimal health status in farm animals.

INTRODUCTION: On discute depuis des années de l'usage des antibiotiques dans l'élevage des animaux de rente, en particulier en ce qui concerne leur utilisation incorrecte et la charge environnementale liée à des résidus d'antibiotiques susceptibles de favoriser l'apparition et la propagation de résistances. Toutefois les antibiotiques sont essentiels pour assurer une lutte efficace et durable contre les maladies d'origine bactérienne. Dans le but d'optimiser l'usage des antibiotiques dans l'élevage des animaux de rente et, par conséquence, de réduire le développement de résistances, AntibioticScout.ch propose une aide à la décision pour un usage prudent de ces substances ("prudent use"). Parallèlement, on attire l'attention sur les traitements adjuvants et sur les mesures de prévention. Des mesures visant à améliorer la santé et le bien-être des animaux en tenant compte des fondements d'une bonne pratique vétérinaire sont des instruments efficaces pour réduire l'usage des antibiotiques. Cette réduction indispensable doit donc être combinée avec des mesures de gestion adéquates dans les élevages. Ce sont en particulier l'hygiène et les conditions d'élevage correctes ainsi que la mise en place de mesures de biosécurité qui sont décisives pour l'optimisation de la santé des troupeaux.

Impact of nutritional programming on the growth, health, and sexual development of bull calves.

The growth, health, and reproductive performance of bull calves are important prerequisites for a successful cattle breeding program. Therefore, several attempts have been made to improve these parameters via nutritional programming. Although an increase in energy uptake during the postweaning period (7-8 mo of age) of the calves leads to a faster growing rate, it has no positive effects on sexual development. In contrast, a high-nutrition diet during the prepubertal period (8-20 wk of age) reduced the age at puberty of the bulls and increased the size and/or weight of the testis and the epididymal sperm reserves. This faster sexual development is associated with an increased transient LH peak, which seems to be mediated by an increase in serum IGF-I concentrations. However, the exact mechanisms responsible for the interaction between nutrition and the subsequent development of the calves are not clear. The sexual development of bull calves depends not only on the nutrition of the calves after birth but also on the feed intake of their mothers during pregnancy. In contrast to the effects of the feed intake of the bull calves, a high-nutrition diet fed to the mother during the first trimester has negative effects on the reproductive performance of their offspring. In conclusion, it has been clearly demonstrated that growth, health, and reproductive performance can be improved by nutritional programming, but further studies are necessary to obtain a better understanding about the mechanisms responsible for this phenomenon.

Intensive rearing of male calves during the first three weeks of life has long-term effects on number of islets of Langerhans and insulin stained area in the pancreas.

Permanent effects of early postnatal nutrition on the development and function of tissues and organs have been previously demonstrated primarily in humans and rodents. The objective of this study in calves was to analyze the impact of rearing conditions during the first 3 wk of life on morphology of insulin-producing pancreatic ß-cells. Forty-two male Holstein calves were raised during the first 3 wk of life either intensively (intensively reared [INT]; ad libitum milk feeding and individual hutches; = 21) or according to an established restrictive rearing protocol (4 L milk/d) during wk 1 in hutches and 720 g/d milk replacer (MR) from d 8 to 21 in group pens (restrictively reared [CON]; = 21). Thereafter, all calves were housed and fed under comparable conditions. Birth weight and weekly BW up to wk 10 were recorded. Plasma glucose, insulin, IGF-1, and GH levels were assessed in wk 1, 2, 3, and 10 of life. Slaughtering took place after 8 mo and pancreatic tissue from the medium body (corpus pancreatic) was removed. The number of islets of Langerhans and the insulin stained area were examined histologically. Total milk intake of INT calves was nearly double the intake in CON calves in the first 3 wk of life ( < 0.01). Daily starter intake during wk 4 to 10 of life did not differ between groups ( = 0.24). During the first 3 wk, the ADG were up to 9 times higher in INT calves compared to CON calves ( < 0.01), yet BW at time of slaughter did not differ ( = 0.18). Intensive rearing led to increased plasma glucose, insulin, and IGF-1 concentrations after 3 wk of life compared with rearing to the established standard protocol (all < 0.05), whereas GH was lower in INT calves during the second week of life. At time of slaughter, the mean number of islets of Langerhans was higher in INT calves compared to CON calves (9.1 ± 0.3 vs. 7.8 ± 0.3; < 0.01). Also, the total insulin stained area per photograph was higher in INT calves compared to CON calves (107,180 ± 4,987 vs. 84,249 ± 4,962 µm; < 0.01). Number of islets of Langerhans was negatively associated with birth weight but positively correlated with insulin and in trend with IGF-1 plasma levels during the second week of life. Insulin stained area tended to be linked with IGF-1 concentration during the third week of life. In conclusion, differences in the morphology of pancreatic islets of Langerhans indicate that calves can be programmed metabolically by an altered postnatal rearing intensity.

Effects of two different rearing protocols for Holstein bull calves in the first 3 weeks of life on health status, metabolism and subsequent performance.

The aim of this study was to investigate the impact of weight gain of calves within the first 3 weeks of life on health status and subsequent performance. Holstein bull calves were reared either intensively (IR; individual hutches and ad libitum milk feeding for the first 3 weeks of life; n = 24), or according to the established protocol [ER; 4 l milk/day in hutches during week 1 and 720 g/day milk replacer (MR) from day 8 to 21 in a group pen; n = 24]. Water, hay and concentrates were freely available to all calves. From week 4, calves of both groups were housed together in a group pen and fed 720 g MR/day; step-down weaning was performed between week 5 and 10. Key metabolic blood parameters were analysed on day 2, 12, 21 and 70 of life. After weaning, all animals were fed concentrates and corn silage until slaughter at an age of 8 months. Within the first 3 weeks, average daily weight gain was threefold higher in IR calves in relation to ER calves (1.28 vs. 0.38 kg/day, p < 0.001). Neither incidence nor duration of scouring differed significantly between groups. Starter intake (week 4-10) was higher in IR calves in relation to ER calves (49.7 vs. 38.0 kg/calf, p = 0.006). Serum glucose, urea, albumin and insulin were higher at an age of 21 days in IR calves in relation to ER calves; no differences were obvious at an age of 70 days. Plasma GH and IGF-I concentrations revealed an uncoupling of the somatotropic axis in ER calves within the first 3 weeks of life. At slaughter, body weight of IR calves tended to be higher than that of the ER calves (320 vs. 309 kg, p = 0.07). In conclusion, intensive feeding and individual housing during the first 3 weeks of life had positive long-term effects on subsequent performance.

Hepatic mRNA expression of acid labile subunit and deiodinase 1 differs between cows selected for high versus low concentrations of insulin-like growth factor 1 in late pregnancy.

The somatotropic axis is a key metabolic pathway during transition from late pregnancy to early lactation in dairy cows. The first objective of this study was to determine the feasibility of selecting cows with persistent differences in total insulin-like growth factor 1 (IGF-1) concentration by taking only a single antepartum blood sample. The second objective was to elucidate the underlying causes of differences in peripheral IGF-1 concentrations throughout late pregnancy and whether hormonal axes also differed in dairy cows with low versus high IGF-1. Twenty clinically healthy Holstein Friesian cows were chosen based on their plasma IGF-1 concentration at 244 to 254 d after artificial insemination (AI) and other selection criteria (health status, body condition score, number of lactations). These cows were selected from a large-scale farm, transported to the clinic, and monitored daily from 261 to 275 d after AI. The concentrations of IGF-1, growth hormone, IGF binding proteins 2, 3, and 4, insulin, cortisol, thyroid hormones, progesterone, and estradiol were measured. Ultimately, 7 IGF-1-low and 7 IGF-1-high cows were statistically analyzed. Additionally, a liver biopsy was taken on d 270 ± 1 after AI for analysis of gene expression of somatotropic family members, liver deiodinase 1, and suppressor of cytokine signaling-2. It was possible to select cows with different IGF-1 concentrations based upon only 1 blood sample collected in late pregnancy. Concentrations of IGF-1 in IGF-1-low versus IGF-1-high animals (n=7 each) remained significantly different between groups from the day of selection of the animals until d 275 after AI. Second, the differences in total plasma IGF-1 concentration between experimental groups may be attributed to differences in hepatic production of acid labile subunit. The ability of IGFBP-3 to bind IGF-1 declined before calving in all cows. Furthermore, in addition to decreased mRNA expression of growth hormone receptor 1A and IGF-1 relative to calving, serum binding capacities for IGF-1 also decreased. Insulin-like growth factor binding protein 4 mRNA expression was higher in cows with low IGF-1 concentrations; this binding protein inhibits IGF-1 action at the tissue level and therefore may reduce IGF-1 bioavailability. Finally, other endocrine end points (e.g., insulin and thyroid hormones) differed between the 2 groups.

Multiparous cows categorized by milk protein concentration and energy-corrected milk yield during early lactation--metabolism, productivity and effect of a short-term feed restriction.

The objective of this experiment was to study milk productivity, metabolic adaptation and effect of a short-term feed restriction (FR) on key performance indicators during early lactation in cows classified according to energy-corrected milk (ECM) yield and milk protein concentration. Twenty-three multiparous Holstein-Friesian cows were categorized in four groups according to respective averaged values on Days 23-25 postpartum: high ECM yield and high protein concentration; low ECM yield and low protein concentration; high ECM yield and low protein concentration and low ECM yield and high protein concentration. Dry matter intake was reduced to 68.3% for three subsequent days. Our results showed that short-time FR in early lactation succeeded in enhancing energy deficit of cows in all groups. Milk fat, milk protein and lactose concentrations as well as milk fat yield were not influenced by FR. Several hepatic genes encoding for enzymes involved in catabolism of amino acids, ß-oxidation, gluconeogenesis and ketogenesis as well as mRNA encoding for insulin receptor showed increased transcript abundances after FR, primarily in cows with high milk yield and low milk protein concentration.

Feeding untreated and pasteurized waste milk and bulk milk to calves: effects on calf performance, health status and antibiotic resistance of faecal bacteria.

Non-saleable milk (waste milk, WM) is contaminated with an undefined spectrum of potentially harmful pathogens and antimicrobial residues. The objective of this study was to determine the impact of feeding bulk milk (BM) or WM - both pasteurized or not - on calf performance, health and the antibiotic resistance of specific faecal bacteria. A total of 114 calves from a large-scale dairy were housed outdoors in individual hutches and were randomly assigned to one of four feeding groups. The calves were fed either WM, pasteurized WM (pWM), BM or pasteurized BM (pBM) from day 3 to 56 of life. Milk samples taken from the pasteurizer and calves' nipple buckets were investigated at regular intervals for total plate count and counts of thermoduric bacteria, coliforms and mastitis pathogens. Faecal samples were taken on days 2, 14, 28 and 56 of life from randomly selected calves of the WM, pWM and BM groups (each N = 8-9) and processed to obtain from each sample preferably two isolates of Escherichia (E.) coli and Enterococcus spp. respectively. Isolates were tested for their antimicrobial susceptibility to 25 antimicrobial agents by broth microdilution. Daily weight gain, milk and calf starter intake and health parameters did not differ significantly between the calves of the four feeding groups. The proportion of resistant E. coli isolates was significantly higher in calves fed WM and in calves fed pWM (most pronounced for cephalosporins) than in calves receiving BM. No differences in resistance were found for Enterococus spp. Thus, the concerns for selecting resistant faecal bacteria by feeding WM seem to be justified. Nonetheless, pasteurized WM of cows not treated with antimicrobials represents an acceptable feed for young calves.

Effects of dexamethasone-21-isonicotinate on peripheral insulin action in dairy cows 5 days after surgical correction of abomasal displacement.

BACKGROUND: Dexamethasone frequently is used for treatment of ketosis in dairy cows, but its effects are not fully understood. HYPOTHESIS: Dexamethasone treatment affects whole body insulin sensitivity. ANIMALS: Twelve German Holstein cows, 2-4 weeks postpartum, 5 days after omentopexy to correct left abomasal displacement. METHODS: Randomized, blinded, case-control study. Treatment with dexamethasone-21-isonicotinate (DG; 40 µg/kg IM; n = 6) or saline (control group [CG], 15 mL IM, n = 6) on day 0 (d0). Blood samples were obtained before (d0) and after treatment (d1 and d2), and analyzed for glucose, insulin, and nonesterified fatty acid (NEFA) concentrations. Hepatic triglycerides (TAG) were measured in liver samples taken on d0 and d2. Five consecutive hyperinsulinemic-euglycemic clamps (HEC-I-V; insulin dosages: 0.1, 0.5, 2, 5, 10 mU/kg/min, respectively) were performed on d1 and steady state glucose infusion rate (SSGIR), insulin concentration (SSIC), insulin sensitivity index (ISI = SSGIR/SSIC), and plasma NEFA concentration (SSNEFA) were assessed. RESULTS: Compared with CG-cows, DG-cows on d1 had higher plasma glucose (P = .004) and insulin (P < .001) concentrations, decreased SSGIR (HEC-II, P = .002; HEC-IV, P = .033), ISI (HEC-I, P < .015; HEC-II, P = .004), and insulin-stimulated decrease in SSNEFA (HEC-II, P = .006; HEC-III, P = .01; HEC-IV, P = .003; HEC-V, P = .011). Decrease in hepatic TAG content in DG-cows was higher compared with CG-cows (P < .001). CONCLUSIONS AND CLINICAL IMPORTANCE: Dexamethasone decreases whole body insulin sensitivity and affects glucose and lipid metabolism in early lactating dairy cows.

[Lactational incidences of production diseases in German Fleckvieh cows of six Bavarian dairy farms]. / Laktationsinzidenzen von Produktionskrankheiten bei Fleckviehkühen in sechs bayerischen Milchviehbetrieben.

OBJECTIVE: The aim of this study was to assess lactation incidences of production diseases in German Fleckvieh cows. MATERIAL AND METHODS: Investigations were carried out on six dairy farms (mean milk yield of herds 2008: 7834 ± 708kg milk [mean ± SD]) in Bavaria. All farms kept the cows in free stall barns and fed them a total or partial mixed ration based on grass silage and corn silage. In total, 116 cows and 58 heifers were examined daily for 14 days post partum and treated - if necessary - according to standard protocols. The acquisition of data for diseases in the further lactation was carried out by regular visits to the farm as well as communication with the herd manager and the farm veterinarian. RESULTS: Pluriparous cows suffered more frequently from production diseases (milk fever, retained placenta, clinical ketosis, abomasal displacement, metritis, endometritis, ovarian cysts, mastitis) than primiparous heifers: 33.3% and 46.4% of pluriparous and primiparous cows, respectively, remained clinically healthy, while 24.8% and 30.4%, respectively, suffered from one production disease during the first 2 weeks of lactation; more than one production disease was diagnosed in 41.9% and 23.2% of pluriparous and primiparous cows, respectively. The lactation incidences of production diseases varied considerably among pluriparous cows of the six farms: retained placenta 16.8 ± 13.2%, milk fever 15.1 ± 7.0%, clinical ketosis 16.8 ± 12.4%, metritis 3.8 ± 3.1%, abomasal displacement 1.1% (median 0.0; 0.0/0.0%), endometritis 11.7 ± 7.0%. Mastitis affected 56.0 ± 7.4% of the pluriparous cows, which experienced 1.7 mastitis episodes on average. At least one follicular cyst was diagnosed among 28.4 ± 8.6% of the cows. Lameness affected 18.5 ± 13.5% of pluriparous cows and heifers during the first 2 weeks of lactation. CONCLUSION: The lactation incidences of production diseases did not significantly differ from reference values reported for Holstein Friesian cows except the lower incidence of LDA among German Fleckvieh cows. The results indicate that the farm management affected lactation incidences of production diseases to a greater degree than additional factors, such as the breed of the cows.

Short communication: Prepartum plasma insulin-like growth factor-I concentrations based on day of insemination are lower in cows developing postpartum diseases.

Because peripartal production diseases are prevalent in dairy cows, early recognition is crucial. Several studies reported metabolic variables as risk predictors for subsequent diseases. To improve on-farm testing and application of those methods, the sampling procedure should take into account variation in gestation length. Furthermore, additional variables indicating cows at risk of any production disease should be sought. Therefore, the objective was to characterize differences between cows with and without postpartum production disease (retained fetal membranes, ketosis, hypocalcemia, abomasal displacement, metritis, mastitis) by prepartum measurement of serum nonesterified fatty acid (NEFA) and plasma insulin-like growth factor (IGF)-I concentrations relative to the artificial insemination (AI) that established pregnancy. Blood was collected from 41 Holstein Friesian cows on 235 to 241, 242 to 248, 249 to 255, 256 to 262, 263 to 269, 270 to 276, 277 to 283, and 284 to 290 d after AI. Health status was assessed daily for 3 wk after calving; 25 cows (66%) had at least one production disease. Cows developing postpartum diseases had higher mean serum NEFA concentrations (450 ± 26 µmol/L; mean ± SE) and lower plasma IGF-I concentrations (78 ± 6 ng/mL) prepartum compared with healthy cows (259 ± 19 µmol/L and 117 ± 8 ng/mL, respectively). In conclusion, because of substantial variation among cows in gestation length, blood samples should be collected and studies performed on risk prediction relative to AI rather than expected date of calving. As the somatotropic axis is one of the key regulators of metabolic adaption for onset of lactation, IGF-I might be a useful variable to differentiate between cows susceptible to production diseases and cows that are able to adapt adequately within the transition period and remain healthy.

[Red discolouration of the urine in a dairy cattle herd as a stock problem]. / Rotfärbung des Harns als Bestandsproblem in einer Milchviehherde. Ein Fallbericht.

After feeding a new batch of rapeseed meal (2.5 kg/cow/day) in the total mixed ration (TMR) of dairy cows on a dairy farm in Bavaria, numerous puddles of reddish fluid were found on the floor of the cubicle housing system. These were caused by a red discolouration of the urine. Directly after urination, the urine was macroscopically yellow and bright; the discolouration developed throughout the consecutive hours. Feed intake was markedly decreased and milk yield was lowered by 10%. No disturbances of the general health and blood key parameters were evident. After feeding two other cows with rapeseed meal of this batch (three times daily 1 kg each), the phenomenon occurred in one animal on the third and fourth days. Further analyses revealed evidence that the discolouration was due to substances which were excreted via the kidney and led to reddish urine after delayed reaction with the oxygen in the air.

Ex vivo phagocytic overall performance of neutrophilic granulocytes and the relation to plasma insulin-like growth factor-I concentrations in dairy cows during the transition period.

Impaired function of polymorphonuclear neutrophilic leukocyte (PMNL) during the peripartal period is a major reason for increased susceptibility of dairy cows to infections in this critical interval. Factors dysregulating PMNL function are widely unknown. Insulin-like growth factor (IGF-I) enhanced PMNL functions in vitro. The objective of this study was to investigate the influence of IGF-I and, additionally, ß-hydroxybutyrate and nonesterified fatty acid concentrations on phagocytic activity (PA, percentage of viable PMNL) and phagocytic capacity (PC, mean fluorescence intensity of phagocytic PMNL) assessed by flow cytometry. Antepartum (i.e., wk -3, -2, -1; before calving), plasma concentrations of IGF-I were high (80-110 ng/mL) without significant differences between primiparous and pluriparous cows (n=18 and n=41, respectively). Concentrations of IGF-I declined toward the week of calving (wk 1). Postpartum (i.e., wk 2, 3, and 4; after calving), IGF-I remained lower than before parturition, with concentrations higher in primiparous compared with those of pluriparous cows. The PA was constant in primiparous cows throughout the study period. Conversely, PMNL of pluriparous cows had a significantly increased and higher PA in wk 2 and 3 postpartum compared with that of primiparous cows. The PC decreased significantly only in primiparous cows the week of calving, whereas the number of PMNL in primiparous cows exceeded that of pluriparous cows significantly. The phagocytic power (PP, a product of PA by PC), but not the phagocytic overall performance (POP, a product of PA, PC, and PMNL number), differed between primiparous and pluriparous cows in wk 3 postpartum. No significant differences in POP were found, except in wk 4 after calving between the primi- and pluriparous cows. In both groups, POP increased in the week of calving (wk 1). In contrast to ß-hydroxybutyrate, which was weakly positive correlated with PA and PP in pluriparous cows in the transition period (wk -3 antepartum to wk 4 postpartum), pluriparous animals had weak negative correlations of PMNL number, PA, PP, POP, and IGF-I concentration in this period. In primiparous animals, only PP and PC were weakly negatively correlated with IGF-I in the transition period. Increased plasma IGF-I concentrations were not associated with enhanced phagocytosis function of bovine blood PMNL ex vivo and, thus, can not be regarded as a suitable predictor for this function.