RESUMO
Gas-liquid chromatography was used to study the composition of fatty acids and hydrocarbons of different types of Mycobacteria, including M. tuberculosis, opportunistic Mycobacteria and acid-fast saprophytes. In terms of composition of fatty acids and hydrocarbons, clinical and laboratory strains of M. tuberculosis are very similar. The cellular higher fatty acids of M. tuberculosis differ much from those of opportunistic Mycobacteria and acid-fast saprophytes. The findings can be used for the identification and differentiation of different types of Mycobacteria.
Assuntos
Líquido da Lavagem Broncoalveolar/microbiologia , Metabolismo dos Carboidratos , Ácidos Graxos/metabolismo , Mycobacterium tuberculosis/classificação , Escarro/microbiologia , Tuberculose Pulmonar/microbiologia , Carboidratos/química , Cromatografia Gasosa , Ácidos Graxos/química , Humanos , Técnicas In Vitro , Mycobacterium tuberculosis/metabolismoRESUMO
The role of intracellular protein fractions, isolated from M. tuberculosis virulent strains in the process of the electrophoretic separation, in the development of delayed hypersensitivity reactions has been studied. Low reactogenicity of protein fractions has been established on the basis of the development of faint immunopathological reactions in sensitized animals. High-molecular fraction 1 may be used as a sensitive preparation for the differentiation of individual mycobacterial species.
Assuntos
Proteínas de Bactérias/imunologia , Hipersensibilidade Tardia/etiologia , Mycobacterium/imunologia , Animais , Proteínas de Bactérias/isolamento & purificação , Adesão Celular/imunologia , Diagnóstico Diferencial , Cobaias , Hipersensibilidade Tardia/diagnóstico , Hipersensibilidade Tardia/patologia , Leucócitos/imunologia , Pulmão/patologia , Infecções por Mycobacterium/diagnóstico , Mycobacterium avium/imunologia , Mycobacterium phlei/imunologia , Mycobacterium tuberculosis/imunologia , Micobactérias não Tuberculosas/imunologia , Teste TuberculínicoAssuntos
Proteínas de Bactérias/análise , Mycobacterium tuberculosis/classificação , Densitometria , Eletroforese em Gel de Poliacrilamida , Mycobacterium bovis/análise , Mycobacterium bovis/classificação , Mycobacterium phlei/análise , Mycobacterium phlei/classificação , Mycobacterium tuberculosis/análise , Especificidade da EspécieAssuntos
Glomerulonefrite/microbiologia , Formas L , Mutação , Animais , Doença Crônica , Humanos , Formas L/isolamento & purificação , CamundongosRESUMO
Bacteriological analysis of urine of 150 patients with chronic pyelonephritis was performed. As a result mycoplasma was isolated from urine of 25 patients. Mycoplasma and Coli bacteria or Proteus were isolated simultaneously from urine of 10 patients. Biochemical properties and sensitivity to antibiotics of 9 isolates were studied. The data provided recommendation of the urine analysis for the presence of mycoplasma.
Assuntos
Mycoplasma/isolamento & purificação , Pielonefrite/microbiologia , Urina/microbiologia , Antibacterianos/farmacologia , Doença Crônica , Meios de Cultura , Humanos , Testes de Sensibilidade Microbiana , Mycoplasma/metabolismo , Resistência às Penicilinas , Pielonefrite/urina , Fatores de TempoRESUMO
The authors present the data concerning the study of 200 patients suffering from chronic pyelonephritis; in 28 of these L-forms of bacteria were revealed in the urine. Of 46 L-cultures isolated from these patients 13 reversed into bacterial forms, 8 failed to reverse and were referred to the stable L-forms; the rest 25 L-cultures perished during the 8th--10th passage. This led to a supposition that the relapses and exacerbations of the infectious process in pyelonephritis were associated with the change of the L-forms into bacterial ones, and that the persistence of the L-forms in the kidney tissue promoted the maintenance of the chronic process.