Effect of Laser Irradiation Modes and Photosensitizer Types on Antimicrobial Photodynamic Therapy (aPDT) for Streptococcus sobrinus in the Crown Dentin of Bovine Teeth: An Experimental In Vitro Study.

This study aimed to assess the impact of different laser irradiation modes and photosensitizer types on the bactericidal efficacy of antimicrobial photodynamic therapy (aPDT). Dentin plates were prepared by sectioning the crown dentin of bovine teeth infected with Streptococcus sobrinus (n = 11). Nine aPDTs involving the combination of three 1% solutions of photosensitizers (brilliant blue, BB; acid red, AR; and methylene blue, MB) and three irradiation modes of semiconductor lasers (50 mW for 120 s, 100 mW for 60 s, and 200 mW for 30 s) were performed for each infected dentin plate, and the control consisted of the specimens not applied with aPDT. The bactericidal effects in 10 groups were evaluated using both assays of the colony count (colony-forming-unit: CFU) and adenosine triphosphate (ATP) (relative-light-unit: RLU). The data obtained were analyzed using the Kruskal-Wallis test (α = 0.05). The most aPDT groups exhibited significantly lower RLU and CFU values compared with the control (p < 0.05). The effect of irradiation modes on RLU and CFU values was significant in the aPDT group using BB (p < 0.05) but not in the aPDT group using AR or MB. The aPDT performed with AR or MB exerted a remarkable bactericidal effect.

Effects of Sr2+, BO33-, and SiO32- on Differentiation of Human Dental Pulp Stem Cells into Odontoblast-Like Cells.

This study aimed to clarify the effects of strontium (Sr2+), borate (BO33-), and silicate (SiO32-) on cell proliferative capacity, the induction of differentiation into odontoblast-like cells (OLCs), and substrate formation of human dental pulp stem cells (hDPSCs). Sr2+, BO33-, and SiO32- solutions were added to the hDPSC culture medium at three different concentrations, totaling nine experimental groups. The effects of these ions on hDPSC proliferation, calcification, and collagen formation after 14, 21, and 28 days of culture were evaluated using a cell proliferation assay, a quantitative alkaline phosphatase (ALP) activity assay, and Alizarin Red S and Sirius Red staining, respectively. Furthermore, the effects of these ions on hDPSC differentiation into OLCs were assessed via quantitative polymerase chain reaction and immunocytochemistry. Sr2+ and SiO32- increased the expression of odontoblast markers; i.e., nestin, dentin matrix protein-1, dentin sialophosphoprotein, and ALP genes, compared with the control group. BO33- increased the ALP gene expression and activity. The results of this study suggested that Sr2+, BO33-, and SiO32- may induce hDPSC differentiation into OLCs.

The bactericidal and biofilm removal effect of super reducing water on Streptococcus mutans in three types of orthodontic brackets.

PURPOSE: To investigate the bactericidal and biofilm removal effect of super reducing water (SRW) on Streptococcus mutans (S. mutans) adhered to orthodontic brackets, in vitro. METHODS: Three types of brackets were bonded to aluminum disks. After the formation of S. mutans biofilms on the surfaces, the brackets were divided into three groups (n = 44 each) based on their exposure to SRW: group 1, no treatment; group 2, treated for 5 min; and group 3, treated for 10 min. Total viable counts, adenosine triphosphate measurements, crystal violet assay, and scanning electron microscopy were used to evaluate the effect of SRW. RESULTS: The bacterial counts in groups 2 and 3 were significantly lower than those in group 1 (P < 0.001); however, no significant differences were observed between groups 2 and 3. Marked decreases in the number of bacterial colonies and extent of biofilm formation were observed in groups 2 and 3 compared to group 1. No significant differences in the number of bacterial colonies and amount of biofilm were observed among the three types of brackets in each group. CONCLUSION: These findings indicate the bactericidal and biofilm removal effect of SRW treatment on S. mutans adhered to orthodontic brackets.

Decompaction wave propagation in a vibrated fine-powder bed.

We experimentally study the crack formation and decompaction-wave propagating in a vibrated powder bed consisting of glass beads of 5 µm in diameter. The vibrated powder bed exhibits three distinct phases depending on the vibration conditions: consolidation (CS), static fracture (SF), and dynamic fracture (DF). Particularly, we found an upward wave propagation in the DF regime when the powder bed is strongly vibrated. As a remarkable feature, we found that in fine cohesive powders, the decompaction-wave propagation speed normalized to gravitational speed is independent of the shaking strength. This result implies that the wave propagation speed is governed by the balance between gravity and cohesion effect rather than vibration strength. We also explore the universality of wave propagation phenomenon in coarser and low-density granular powders.

History-dependent growth and reduction of the ripples formed on a swept granular track.

When a solid object or wheel is repeatedly dragged on a dry sandy surface, ripple patterns are formed. Although the conditions to form ripple patterns have been studied well, methods to eliminate the developed ripple patterns have not been understood thus far. Therefore, history-dependent stability of the ripple patterns formed on a sandy surface is investigated in this study. First, the ripple patterns are formed by sweeping the flat sandy surface with a flexible plow at a constant speed. Then, the sweeping speed is reduced, and the variation of ripple patterns is measured. As a result, we find that the ripple patterns show hysteresis. Specifically, the increase in amplitude of ripples is observed when the reduced velocity is close to the initial velocity forming the ripple pattern. In addition, splitting of ripples is found when the reduced velocity is further decreased. From a simple analysis of the plow's motion, we discuss the physical mechanism of the ripple splitting.

Effects of toothbrush abrasion on surface and antibacterial properties of hydroxyapatite-tryptophan complex with gray titania.

Photocatalysts have attracted attention in the medical field for their antibacterial effects. However, typical photocatalysts are activated by ultraviolet rays, which may have adverse effects. Therefore, we focused on a new photocatalyst that is activated by visible light, hydroxyapatite (HAp), and amino acid complex with gray titania, and evaluated its antibacterial effects against Porphyromonas gingivalis and effect by toothbrushing. The test sample was a titanium alloy substrate, and four surface treatments were applied: (1) substrate only, (2) substrate with HAp complex, (3) substrate with HAp complex with gray titania, and (4) HAp-tryptophan complex with gray titania (TR). These surface treatments were evaluated with or without toothbrushing (8 total groups). Surface roughness (Sa), fluorescent X-ray analysis (XRF), and scanning electron microscopy (SEM) were used to evaluate surface properties. To investigate antibacterial effects, each sample was seeded with P. gingivalis, irradiated with red light, and total viable bacterial count was determined. For Sa measurement, TR showed no significant difference after toothbrushing. However, in XRF and SEM observation, TR exhibited peeling of the applied coating after toothbrushing. In the antibacterial test, TR showed a decrease in P. gingivalis under no toothbrushing condition. Conversely, with toothbrushing, the TR coating appeared to peel. However, no significant difference in P. gingivalis count was observed among all groups. HAp-tryptophan complex with gray titania coating showed an antibacterial effect against P. gingivalis when irradiated with visible light. However, toothbrushing can result in coat peeling and consequently reduce the antibacterial effect.

Deformation of a rotated granular pile governed by body-force-dependent friction.

Although the gravity dependence of granular friction is crucial to understand various natural phenomena, its precise characterization is difficult. We propose a method to characterize granular friction under various gravity (body force) conditions controlled by centrifugal force; specifically, the deformation of a rotated granular pile was measured. To understand the mechanics governing the observed nontrivial deformation of this pile, we introduced an analytic model considering local force balance. The excellent agreement between the experimental data and theoretical model suggests that the deformation is simply governed by the net body force (sum of gravity and centrifugal force) and friction angle. The body-force dependence of granular friction was precisely measured from the experimental results. The results reveal that the grain shape affects the degree of body-force dependence of the granular friction.

Bactericidal effect of antimicrobial photodynamic therapy (aPDT) on dentin plate infected with Lactobacillus acidophilus.

This study aimed to examine bactericidal effects of a new antimicrobial photodynamic therapy (aPDT) on dentin plates infected with Lactobacillus acidophilus (L. acidophilus). First, we measured the amount of reactive oxygen species (ROS) produced when new photosensitizer (PS), acid red (AR), and brilliant blue (BB) were irradiated with a semiconductor laser. ROS generated from each PS solution by laser irradiation was calculated as the total light emission amount (Relative Light Unit, RLU) using a chemiluminescence measuring device. Second, we examined bactericidal effects of the aPDT on dentin plates infected with L. acidophilus. The bactericidal effects on each group were evaluated by colony count assay and adenosine triphosphate assay. The experimental groups comprised two laser irradiation groups (650 nm laser, 650laser; and 940 nm laser, 940laser), two PS groups (BB and AR), four aPDT groups (650 nm laser irradiation with BB, 650laser-BB; 650 nm laser irradiation with AR, 650laser-AR; 940 nm laser irradiation with BB, 940laser-BB; 940 nm laser irradiation with AR, 940laser-AR), and a control. The ROS in all aPDT groups was significantly higher than in the control. RLU in all groups applied with laser irradiation was significantly lower than that in the control. However, only 650laser-BB showed significantly lower colony counts than the control. 650laser-BB was the most effective in sterilizing the infected dentin plates.

Antibacterial activity of blue high-power light-emitting diode-activated flavin mononucleotide against Staphylococcus aureus biofilm on a sandblasted and etched surface.

BACKGROUND: Because of high affinity to the titanium implant surface, Staphylococcus aureus (S. aureus) has been reported as key microorganism that cause the peri-implantitis, even though it is not the typical periodontal pathogenic bacterial strain. The aim of this study was to evaluate the antibacterial property of the aPDT device, using blue high-power LED light activated flavin mononucleotide, comparing to the previously proven aPDT method using methylene blue and red laser on S. aureus biofilm. METHODS: Commercial pure titanium grade 4 modified surface with SLA were used to form S. aureus biofilm for 48 h. Two aPDT systems were used in this study; 1) HELBO®Blue Photosensitizer (Bredent medical), which is methylene blue (MB) activated by 670-nm red diode laser and 2) FotoSan® Blue agent Gel (CMS Dental), which contains flavin mononucleotide (FMN) activated by FotoSan® BLUE LAD (Light Activated Disinfection) light. The antibacterial tests were performed by total viable count, crystal violet assay, and direct observation methods. RESULTS: Using the light activated-PS, the log reduction in CFU/mL compared to non-treatment was 1.23 ±â€¯0.19 log10 and 1.23 ±â€¯0.12 log10 (about 93 % of reduction) for MB and FMN, respectively. The significant difference in the reduction could be determined when comparing with using only light (p < 0.01). Regarding two aPDT systems, the decrease in amount of bacteria after treatment was not significantly different (p > 0.05). CONCLUSION: The antibacterial activities of aPDT using blue high-power LED light activated flavin mononucleotide on S. aureus biofilm was comparable to those of previous research supporting aPDT using photoactivated MB.

The Antibacterial Activity of Hydroxyapatite-Tryptophan Complex with Gray Titania by Photocatalysis Using LED Diodes.

PURPOSE: Peri-implantitis is an important biologic complication that can lead to implant failure. Proper treatment should effectively kill bacteria, not harm the implant surface, and promote regeneration. Recently, photocatalytic coating without antibiotics or external agents was proposed to be an alternative to antibiotic therapy. The aim of this study was to evaluate the photocatalytic antibacterial effect of a new titanium implant coating made from hydroxyapatite-tryptophan complex and gray titania, which was activated by two visible lights. MATERIALS AND METHODS: Titanium alloy substrate was plasma sprayed with hydroxyapatite (80 wt%) and dititanium trioxide (20 wt%) and then pressed with tryptophan. Three bacteria related to peri-implantitis, Porphyromonas gingivalis, Tannerella forsythia, and Aggregatibacter actinomycetemcomitans, were used in this study. Six conditions were tested: (1) control group (only bacteria), (2) photocatalytic sample in darkness (bacteria and coated sample in darkness), (3) red laser for 15 minutes (bacteria irradiated with photoactivated disinfection [PAD] light, 650-nm wavelength), (4) broadband light-emitting diode (LED) for 15 minutes (bacteria irradiated with broadband LED, peak wavelength at 470 nm), (5) photocatalysis by red laser for 15 minutes (bacteria and coated sample irradiated with PAD light), and (6) photocatalysis by broadband LED for 15 minutes (bacteria and coated sample irradiated with broadband LED). After 15 minutes of irradiation, photocatalytic antibacterial effects were evaluated by total viable bacterial count, adenosine triphosphate (ATP) assay, and LIVE/DEAD assay. RESULTS: The number of all bacteria tested was significantly decreased by the photocatalytic effect of both visible light sources (P < .05). For P gingivalis, viable bacteria of lethal photosensitization groups were also significantly decreased (P < .05), especially when using the broadband LED. However, the coating material itself did not have antibacterial properties without light activation. There was no significant difference in ATP among groups (P > .05). LIVE/DEAD staining showed that red fluorescent bacterial cells were present in photocatalytic groups from the two light sources. CONCLUSION: Photoactivated hydroxyapatite-tryptophan complex and gray titania as a photocatalytic coating has antibacterial effects on bacteria associated with peri-implantitis.

A novel experimental setup for an oblique impact onto an inclined granular layer.

We develop an original apparatus of the granular impact experiment by which the incident angle of the solid projectile and the inclination angle of the target granular layer can be systematically varied. Whereas most of the natural cratering events occur on inclined surfaces with various incident angles, there have not been any experiments on oblique impacts on an inclined target surface. To perform systematic impact experiments, a novel experimental apparatus has to be developed. Therefore, we build an apparatus for impact experiments where both the incident angle and the inclination angle can be independently varied. The projectile-injection unit accelerates a plastic ball (6 mm in diameter) up to vi ≃ 100 m s-1 impact velocity. The barrel of the injection unit is made with a three-dimensional printer. The impact dynamics is captured by using high-speed cameras to directly measure the impact velocity and incident angle. The rebound dynamics of the projectile (restitution coefficient and rebound angle) is also measured. The final crater shapes are measured using a line-laser profiler mounted on the electric stages. By scanning the surface using this system, a three-dimensional crater shape (height map) can be constructed. From the measured result, we can define and measure the characteristic quantities of the crater. The analyzed result on the restitution dynamics is presented as an example of systematic experiments using the developed system.

Laboratory experiment and discrete-element-method simulation of granular-heap flows under vertical vibration.

Granular flow dynamics on a vertically vibrated pile is studied by means of both laboratory experiments and numerical simulations. As already revealed, the depth-averaged velocity of a fully fluidized granular pile under strong vibration, which is measured by a high-speed laser profiler in the experiment, can be explained by the nonlinear diffusion transport model proposed by our previous paper [Tsuji et al., Phys. Rev. Lett. 120, 128001 (2018)PRLTAO0031-900710.1103/PhysRevLett.120.128001]. In this paper, we report that a similar transport model can be applied to the relation between the surface velocity and slope in the experiment. These facts are also reproduced by particle-scale numerical simulations based on the discrete element method. In addition, using these numerical results, the velocity profile inside the fluidized pile is measured. As a result, we show that the flow velocity decreases exponentially with depth from the surface of the pile, which means that a clearly fluidized region, also known as shear band structure, is localized around the surface. However, its thickness grows proportionally with the local height of the pile, i.e., the shear band does not consist of a fluidized layer with a constant thickness. From these features, we finally demonstrate that the integration of this exponentially decreasing velocity profile is consistent with the depth-averaged velocity predicted by the nonlinear diffusion transport model.

Physical constraints on sand crab burrows: Mechanical properties of wet sand explain the size and spatial distributions of burrows on beaches.

The diameter and vertical depth of sand crab tunnels in sandy beaches are usually restricted to a few centimeters scale and several tens of centimeters, respectively. We designed a study to determine what physical factors restrict tunnel diameter and predict the maximum attainable tunnel diameter and depth. We collected field data on the size and spatial distributions of ghost crab (Ocypode spp.) burrows on two sandy beaches (Kawage Beach in Tsu, Mie Prefecture, Japan and Sakieda Beach in Ishigaki, Okinawa Prefecture, Japan), where O. ceratophthalma dominants the ghost crab fauna. We measured burrow depths and distance from shoreline in concert with water content of sandy beaches. To explain our observed distributions of crab burrows in the field, we performed experiments in a lab microcosm, comprising a horizontal tunnel through wet sand. We measured the static stability of tunnel structures in relation to water content and two strengths computed from loading force exerted on the sand overlying the tunnels. By comparing field and experimental data, we found that crabs construct their burrows in appropriately wet zones (wet enough to provide sufficient cohesion of the sand grains in tunnel walls to prevent collapse) and that tunnel diameters and depths are sufficiently small to prevent deformation and collapse of their tunnels.

Cytotoxicity of hydroxyapatite-tyrosine complex with gray titania coating on titanium alloy surface to L929 mouse fibroblasts.

Hydroxyapatite particles (HAp) have been widely used by many dental implant systems as an implant coating material because of their osteoconductive properties. This study aimed at improving the antibacterial effect of HAp as a substitute for antibiotic agents which can increase drug resistance. HAp/gray titania was selected as the coating material for on the titanium alloy substrate due to its antibacterial properties after photocatalytic reaction. When combined with amino acids, HAp can form a fluorescent complex which enhances this property. Before clinical application, this new coating should be examined for cytotoxic effects against biological cells or tissues. Therefore, L929 mouse fibroblasts were used to represent fibrous tissue surrounding dental implant. After performing a 6-day alamarBlue assay, the new coating method using hydroxyapatite-tyrosine complex with gray titania on titanium alloy surface can be said to have no influence on the growth of fibroblasts.

Impact-Induced Energy Transfer and Dissipation in Granular Clusters under Microgravity Conditions.

The impact-induced energy transfer and dissipation in granular targets without any confining walls are studied by microgravity experiments. A solid projectile impacts into a granular target at low impact speed (0.045≤v_{p}≤1.6 m s^{-1}) in a laboratory drop tower. Granular clusters consisting of soft or hard particles are used as targets. Porous dust agglomerates and glass beads are used for soft and hard particles, respectively. The expansion of the granular target cluster is recorded by a high-speed camera. Using the experimental data, we find that (i) a simple energy scaling can explain the energy transfer in both soft-particle and hard-particle granular targets, (ii) the kinetic impact energy is isotropically transferred to the target from the impact point, and (iii) the transferred kinetic energy is 2%-7% of the projectile's initial kinetic energy. The dissipative-diffusion model of energy transfer can quantitatively explain these behaviors.

Void structure stability in wet granular matter and its application to crab burrows and cometary pits.

Cohesive granular matter can support stable void structures, which can universally be found in various scenes from everyday lives to space. To quantitatively characterize the stability and strength of a void structure in cohesive granular matter, we perform a simple tunnel-compression experiment with wet granular matter. In the experiment, a horizontal tunnel in a wet granular layer is vertically compressed with a slow compression rate. The experimental result suggests that the tunnel deformation can be classified into the following three types: (i) shrink, (ii) shrink with collapse, and (iii) subsidence by collapse. Using the experimental result, we estimate the stable limit of various void structures in a cohesive granular layer from crab burrows on a sandy beach to the pits observed on cometary surfaces.

Force-chain evolution in a two-dimensional granular packing compacted by vertical tappings.

We experimentally study the statistics of force-chain evolution in a vertically-tapped two-dimensional granular packing by using photoelastic disks. In this experiment, the tapped granular packing is gradually compacted. During the compaction, the isotropy of grain configurations is quantified by measuring the deviator anisotropy derived from fabric tensor, and then the evolution of force-chain structure is quantified by measuring the interparticle forces and force-chain orientational order parameter. As packing fraction increases, the interparticle force increases and finally saturates to an asymptotic value. Moreover, the grain configurations and force-chain structures become isotropically random as the tapping-induced compaction proceeds. In contrast, the total length of force chains remains unchanged. From the correlations of those parameters, we find two relations: (i) a positive correlation between the isotropy of grain configurations and the disordering of force-chain orientations, and (ii) a negative correlation between the increasing of interparticle forces and the disordering of force-chain orientations. These relations are universally held regardless of the mode of particle motions with or without convection.

Relaxation Dynamics of a Granular Pile on a Vertically Vibrating Plate.

Nonlinear relaxation dynamics of a vertically vibrated granular pile is experimentally studied. In the experiment, the flux and slope on the relaxing pile are measured by using a high-speed laser profiler. The relation of these quantities can be modeled by the nonlinear transport law assuming the uniform vibrofluidization of an entire pile. The fitting parameter in this model is only the relaxation efficiency, which characterizes the energy conversion rate from vertical vibration into horizontal transport. We demonstrate that this value is a constant independent of experimental conditions. The actual relaxation is successfully reproduced by the continuity equation with the proposed model. Finally, its specific applicability toward an astrophysical phenomenon is shown.

Effect of antimicrobial photodynamic therapy (aPDT) on the sterilization of infected dentin in vitro.

The aim of this study was to evaluate the bactericidal effect of antimicrobial photodynamic therapy (aPDT) on an infected dentin model. Dentin plates were prepared from extracted human molars and infected through immersion in a solution of Streptococcus mutans. The nine experimental groups consisted of two laser irradiation groups (650 nm laser: 650 laser and 940 nm laser: 940 laser), two photosensitizer groups (methylene blue: MB, and azulenocyanine: Azc), four aPDT groups (650 nm laser irradiation of MB: 650 laser-MB, 650 nm laser irradiation of Azc: 650 laser-Azc, 940 nm laser irradiation of MB: 940 laser-MB and 940 nm laser irradiation of Azc: 940 laser-Azc) and a control. The bactericidal effects on each group were evaluated by colony count and adenosine triphosphate (ATP) assays. Based on the results of the colony count assay, the 650 laser-MB and 940 laser-MB groups formed significantly fewer colonies than the other experimental groups. Significantly fewer colonies were observed in the 940 laser-Azc group than in the control, but significant differences in the numbers of colonies were not observed between the 650 laser-Azc and control groups. The 940 laser group formed slightly fewer colonies than the 650 laser group, but the difference was not significant. In addition, the number of colonies in the MB group was significantly less than the number in the Azc group. The results of the ATP assay were similar to those of the colony count assay. aPDT with MB showed a significant bactericidal effect on dentin plates infected with S. mutans.

Temporal analysis of acoustic emission from a plunged granular bed.

The statistical property of acoustic emission (AE) events from a plunged granular bed is analyzed by means of actual-time and natural-time analyses. These temporal analysis methods allow us to investigate the details of AE events that follow a power-law distribution. In the actual-time analysis, the calm-time distribution, and the decay of the event-occurrence density after the largest event (i.e., the Omori-Utsu law) are measured. Although the former always shows a power-law form, the latter does not always obey a power law. Markovianity of the event-occurrence process is also verified using a scaling law by assuming that both of them exhibit power laws. We find that the effective shear strain rate is a key parameter to classify the emergence rate of power-law nature and Markovianity in granular AE events. For the natural-time analysis, the existence of self-organized critical states is revealed by calculating the variance of natural time χ(k), where kth natural time of N events is defined as χ(k)=k/N. In addition, the energy difference distribution can be fitted by a q-Gaussian form, which is also consistent with the criticality of the system.