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1.
J Dent Educ ; 2024 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-39350352

RESUMO

OBJECTIVE: To enhance primary care dentistry and medicine residents' didactic knowledge, communication skills to address patient's healthcare needs, and collaboration among healthcare professionals, while increasing confidence in integrating primary care skills into their clinical practice. METHODS: An Interprofessional Education workshop was organized to enhance didactic knowledge and learning of 42 primary care dentistry (PCD) and family and community medicine (FCM) residents on topics including addressing facial and dental trauma in adults, managing patients with alcohol use and abuse, pain control and prescription opioid use in dentistry, and opioid use disorder and treatment. An online survey was administered before and after the workshop to assess changes in knowledge, confidence, and future intentions to incorporate targeted primary care skills learned during the workshop into clinical practice and to elicit residents' perceptions of workshop quality. Overall scale means were assessed by chi-square tests for knowledge scales and analysis of variance for confidence and career intentions scales, significance levels were set at 0.05. RESULTS: Pre-workshop assessments were completed by all attendees while post assessments received a response rate of 83.3%. Both PCD and FCM residents reported an increase in their knowledge (p < 0.05) and confidence (p < 0.05) in providing care for patients with facial/dental trauma, identifying signs of opioid abuse, addressing alcohol abuse, and understanding the referral processes following the workshop. While an overall increase was noted regarding intentions to incorporate skills learned during the workshop into practice by the PCD (pre-course = 4.33, post-course = 4.42) and FCM (pre-course = 4.55, post-course = 4.73) residents, the differences were not statistically significant differences. CONCLUSIONS: The interprofessional education workshop for PCD and FCM residents enhanced their knowledge, confidence, and future intentions to incorporate primary care skills into clinical practice and improve patient outcomes.

4.
Sci Rep ; 11(1): 12642, 2021 06 16.
Artigo em Inglês | MEDLINE | ID: mdl-34135397

RESUMO

Malnutrition affects growth and development in humans and causes socio-economic losses. Normal maize is deficient in essential amino acids, lysine and tryptophan; and vitamin-A. Crop biofortification is a sustainable and economical approach to alleviate micronutrient malnutrition. We combined favorable alleles of crtRB1 and lcyE genes into opaque2 (o2)-based four inbreds viz. QLM11, QLM12, QLM13, and QLM14 using marker-assisted backcross breeding. These are parents of quality protein maize versions of two elite hybrids viz. Buland and PMH1, grown in India. Gene-based SSRs for o2 and InDel markers for crtRB1 and lcyE were successfully employed for foreground selection in BC1F1, BC2F1, and BC2F2 generations. The recurrent parent genome recovery ranged from 88.9 to 96.0% among introgressed progenies. Kernels of pyramided lines possessed a high concentration of proA (7.14-9.63 ppm), compared to 1.05 to 1.41 ppm in the recurrent parents, while lysine and tryptophan ranged from 0.28-0.44% and 0.07-0.09%, respectively. The reconstituted hybrids (RBuland and RPMH1) showed significant enhancement of endosperm proA (6.97-9.82 ppm), tryptophan (0.07-0.09%), and lysine (0.29-0.43%), while grain yield was at par with their original versions. The dissemination of reconstituted hybrids holds significant promise to alleviate vitamin-A deficiency and protein-energy malnutrition in developing countries.


Assuntos
Biofortificação/métodos , Zea mays/genética , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Grão Comestível/genética , Grão Comestível/metabolismo , Endosperma/metabolismo , Genes de Plantas , Marcadores Genéticos , Humanos , Liases Intramoleculares/genética , Liases Intramoleculares/metabolismo , Lisina/metabolismo , Melhoramento Vegetal/métodos , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo , Triptofano/metabolismo , Vitamina A/metabolismo , Deficiência de Vitamina A/prevenção & controle , beta Caroteno/metabolismo
5.
Mol Biol Rep ; 48(5): 4383-4396, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-34091816

RESUMO

Cotton Leafhopper, Amrasca biguttula is an important pest of cotton and okra in the Indian subcontinent. Presently limited genomic/transcriptomic information is available for this insect in any of open source databases. The present study reports the first assembled and annotated de novo transcriptome of cotton leafhopper. Out of 75,551 transcripts, 39,613 CDS (Coding Sequence) were predicted with 35,282 showing positive blast hits with NCBI nr database. The Gene ontology (GO) analysis annotated 7431 CDS  with KEGG pathway categorizing these CDS into 22 different functional groups. The majority of CDS were annotated in signal transduction and transport catabolism pathways. The sequence data was screened for RNAi pathway genes and presence of 37 transcripts associated with this process confirmed the existence of robust RNAi machinery. The role of core RNAi machinery genes (Dicer-2, Ago-2, Piwi and Staufen) has been validated through dsRNA feeding studies. The data resource has also been used to identify potential RNAi targets and genes associated with insecticide detoxification specifically CYP 450 family. The current study provides a useful sequence resource which can be used to initiate molecular studies in this insect with emphasis on insecticide resistance, RNAi and functional genomics.


Assuntos
Sistema Enzimático do Citocromo P-450/genética , Perfilação da Expressão Gênica/métodos , Genes de Insetos , Hemípteros/genética , Interferência de RNA , Transcriptoma/genética , Animais , Feminino , Ontologia Genética , Gossypium , Hemípteros/crescimento & desenvolvimento , Resistência a Inseticidas/genética , Inseticidas , Masculino , Anotação de Sequência Molecular/métodos , Fases de Leitura Aberta , Filogenia
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