Inhibition of biofilm, quorum-sensing, and swarming motility in pathogenic bacteria by magnetite, manganese ferrite, and nickel ferrite nanoparticles.

Resistance to antibiotics by pathogenic bacteria constitutes a health burden and nanoparticles (NPs) are being developed as alternative and multipurpose antimicrobial substances. Magnetite (Fe3O4 np), manganese ferrite (MnFe2O4 np) and nickel ferrite (NiFe3O4 np) NPs were synthesized and characterized using thermogravimetric analysis, transmission electron microscopy, Fourier transformed infra-red, and X-ray diffraction. The minimal inhibitory concentrations (MIC) ranged from 0.625 to 10 mg/mL against gram-positive (Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), gram-negative (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853) and candida (Candida albicans ATCC 10239 and Candida tropicalis ATCC 13803) species. The NPs exhibited violacein inhibition against Chromobacterium violaceum CV12472 of 100% at MIC and reduced to 27.2% ± 0.8% for magnetite NPs, 12.7% ± 0.3% for manganese ferrite NPs and 43.1% ± 0.2% for nickel ferrite NPs at MIC/4. Quorum-sensing (QS) inhibition zones against C. violaceum CV026 were 12.5 ±0.6 mm for Fe3O4 np, 09.1 ± 0.5 mm for MnFe3O4 NP and 17.0 ± 1.2 mm for NiFe3O4 np. The NPs inhibited swarming motility against P. aeruginosa PA01 and biofilm against six pathogens and the gram-positive biofilms were more susceptible than the gram-negative ones. The NiFe2O4 np had highest antibiofilm activity against gram-positive and gram-negative bacteria as well as highest QS inhibition while Fe3O4 NP had highest biofilm inhibition against candida species. The synthesized magnetic NPs can be used in developing anti-virulence drugs which reduce pathogenicity of bacteria as well as resistance.

A pre-evaluation of psychological factors may positively affect the outcomes of smoking cessation treatments: A comparison in terms of smoking behavior.

INTRODUCTION: The aim of this study was to compare smoking behavior in out-patients in terms of psychological factors with a view to supporting cessation treatment. METHODS: We conducted a cross-sectional and analytical study through face-to-face interviews by the primary care physician with 765 volunteer participants who applied to our hospital for any reason between March and July 2019. The questionnaire administered had two parts: questions about sociodemographic characteristics and usage of tobacco and tobacco products, and questions of the UCLA Loneliness Scale, Multidimensional Scale of Perceived Social Support (MSPSS), Warwick-Edinburgh Mental Wellbeing Scale (WEMBS), and Fagerström Test for Nicotine Dependence (FTND). RESULTS: The study was completed with 765 participants of which 53.1% (n=406) were female and 46.9% (n=359) male. Multidimensional perceived social support scale mean score of the participants was 69.9±15.2 (min=12, max=84). There was a significant relation between mean MPSS and mean WEMBS (p<0.05). As nicotine scores increased, the mean scores both in MPSS and WEMBS decreased (p<0.05). CONCLUSIONS: We suggest that the results of the psychometric preliminary evaluations should be customized for individuals applying to smoking cessation clinics and that including the individual's close social connections in the process can facilitate the decision to quit, thus increase smoking cessation rates.

Lower urinary tract symptoms and related factors in female university students.

OBJECTIVES: Our study aims to determine the symptoms and frequency of lower urinary tract in female university students and its related factors. METHODS: This cross-sectional study was conducted between January and June 2017. To determine their sociodemographic characteristics and toilet habits, the Bristol Female Lower Urinary Tract Symptoms questionnaire, a 21-item questionnaire, was applied to female university students accepting to participate in the study using the face-to-face interview method. RESULTS: The study was completed with 1091 individuals. The mean age of the participants was 20.27±1.69 years. The question "Do you feel a sudden urination sensation and run to the toilet?" was answered by 1.1% (n: 12) of the participants with "Always," while 34.8% (n: 380) replied "Never." In terms of feeling a sudden urination sensation, 0.5% (n: 6) of the participants stated that they "always" experience micturition, while 15.0% (n: 164) answered with "Seldom." The complaint of being unable to empty their bladder completely while urinating was higher in students with constipation problems (P .04) and a body mass index (BMI) > 24.9 (P .02). CONCLUSIONS: Lower urinary tract complaints are a common problem. We believe that routine medical screening should be performed for female university students whose living conditions change when they start university; besides, we suggest that well-attended and repeatable training programs for healthy living behaviors, urinary habits, and hygiene should be organized for university students.

Prevalence of comorbid diseases in patients with fibromyalgia: A retrospective cross-sectional study.

OBJECTIVE: To examine the prevalence of comorbid conditions in patients diagnosed with fibromyalgia. METHODS: The retrospective cross-sectional study was conducted at Eskisehir Osmangazi University, Eskisehir, Turkey, and comprised data of fibromyalgia patients aged 18 years or more admitted between January 1, 2012 and August 15, 2016. Hospital's database was investigated using the International Classification of Diseases, 10th Revision codes to identify fibromyalgia cases and predetermined comorbid conditions. SPSS 21 was used for data analysis. RESULTS: Of 509 patients, 51(10%) were males and 458(90%) were females with an overall mean age of 50.24±12.32 years. Of the total, 345(67.8%) patients had at least one comorbid disease, while 164(32.2%) had no comorbid disease. The most prevalent condition was cardiovascular diseases in 187(36.7%) patients followed by endocrine diseases in 157(30.8%). CONCLUSIONS: Fibromyalgia is a disease that is seen to be increasing in frequency in recent years. It is useful to evaluate fibromyalgia patients with their comorbid conditions on their follow-up.