RESUMO
Utilizing a two-beam technique in the frequency domain, the pumped absorption of PS II membrane fragments from spinach and of acetonic chlorophyll-a solutions was measured at room temperature. In a very narrow wavelength region (0.2 nm around the pump pulse wavelength) the relative test beam transmission exhibited either a decrease or an increase, respectively, dependent on the intensity of a strong pump beam. In contrast, the transmission changes of chl-a solutions were not affected by the wavelength mistuning between pump and test beam. The data obtained for PS II membrane fragments were interpreted in terms of excited state absorption of pigment-protein clusters within the light-harvesting complex of PS II. The interpretation of the small absorption band as a homogeneously broadened line led to a transversal relaxation time for chlorophyll in vivo of about 1 ps.
RESUMO
From numerous investigations it is known that lithium carbonate promotes granulocytopoiesis by stimulation of CSF (colony stimulating factor) in bone marrow. To prove if no immature, in their functions restricted cells are delivered from bone marrow, the activity of granulocytes was tested in vitro in patients with lithium therapy. It could be seen that granulocytes of peripheral blood show an increased in-vitro-activation after lithium influence in vivo.
Assuntos
Disgerminoma/radioterapia , Granulócitos/efeitos dos fármacos , Lítio/uso terapêutico , Neoplasias Testiculares/radioterapia , Disgerminoma/sangue , Granulócitos/efeitos da radiação , Humanos , Carbonato de Lítio , Masculino , Neoplasias Testiculares/sangueRESUMO
In our investigations on rats was proofed the qualification of substances 2-Cyanoethyl Urea (CEH), Thymus-Extract and Lithium-carbonate for a potential reducing or shortening of the leucocyte-depression after whole-body irradiation. Intravenous applications of 2-Cyanoethyl Urea in Wistar rats showed no effect not only in the increase of leucocytes but also in variation in portions of leucocytes. In our investigations we could not influence radiogenic phase of leucopenia by application of CEH after 7 Gy whole-body irradiation. Intramuscular injections of thymus extract don't influence the number of leucocytes in peripheral blood in irradiated Wistar rats. The differential blood-count was uninfluenced. Increase in concentration of thymus extract and also higher frequency of the applications effect no changes in blood-count. Leucocyte depression after whole-body irradiation was independent of the application modus of thymus extract. Lithium-carbonate shows a significant increase of leucocytes in peripheral blood in dependence of dosage and frequency of application. After whole-body irradiation with 7 Gy under lithium therapy it was shown that on day 6th after irradiation leucocyte number was significantly higher than in controls. Radiogenic leucopenia phase was reduced significantly by lithium.
Assuntos
Leucopenia/tratamento farmacológico , Lítio/uso terapêutico , Extratos do Timo/uso terapêutico , Ureia/análogos & derivados , Aerossóis , Animais , Feminino , Injeções Intramusculares , Injeções Intravenosas , Leucopenia/etiologia , Leucopenia/prevenção & controle , Lítio/administração & dosagem , Carbonato de Lítio , Masculino , Ratos , Ratos Endogâmicos , Extratos do Timo/administração & dosagem , Ureia/administração & dosagem , Ureia/uso terapêutico , Irradiação Corporal TotalRESUMO
In our investigations the qualification of the substances 2-cyanoethyl urea, thymus extract and lithium carbonate was tested for a potential reducing or shortening of the leukocyte-depression in rats after whole-body irradiation. Intravenous applications of 2-cyanoethyl urea and intramuscular injections of thymus extract showed no effect in Wistar rats not only in increase of leukocyte number of peripheral blood, but also in variation of leukocyte portion in differential blood-count. Leukocytes depression appearing in consequence of whole-body irradiation was independent of cyanoethyl urea applications and of thymus extract, too. Lithium carbonate shows a significant increase of leukocytes in peripheral blood in dependence of dosage and frequency of applications. After whole-body irradiation with 7 Gy under lithium therapy, it was shown that on day 6 after irradiation leukocyte number was significantly higher than in controls. Radiogenic leukopenia phase was reduced significantly by lithium.
Assuntos
Leucócitos/efeitos da radiação , Lítio/farmacologia , Extratos do Timo/farmacologia , Ureia/análogos & derivados , Irradiação Corporal Total , Animais , Feminino , Contagem de Leucócitos , Leucócitos/efeitos dos fármacos , Carbonato de Lítio , Masculino , Ratos , Ratos Endogâmicos , Ureia/farmacologiaAssuntos
Linfócitos/efeitos da radiação , Animais , Linfócitos B/efeitos da radiação , Feminino , Humanos , Ativação Linfocitária/efeitos da radiação , Camundongos , Tolerância a Radiação , Linfócitos T Citotóxicos/efeitos da radiação , Linfócitos T Auxiliares-Indutores/efeitos da radiação , Linfócitos T Reguladores/efeitos da radiaçãoAssuntos
Hematopoese/efeitos da radiação , Lesões por Radiação/terapia , Anabolizantes/uso terapêutico , Animais , Transfusão de Sangue , Ciclofosfamida/uso terapêutico , Endotoxinas/uso terapêutico , Transfusão de Eritrócitos , Hematopoese/efeitos dos fármacos , Humanos , Lítio/uso terapêutico , Carbonato de Lítio , Camundongos , Nutrição Parenteral , Extratos do Timo/uso terapêutico , Ureia/análogos & derivados , Ureia/uso terapêuticoAssuntos
Antineoplásicos/administração & dosagem , Neoplasias Experimentais/tratamento farmacológico , Radiossensibilizantes/administração & dosagem , Alquilantes/administração & dosagem , Animais , Clorambucila/administração & dosagem , Cisplatino/administração & dosagem , Cricetinae , Ciclofosfamida/administração & dosagem , Doxorrubicina/administração & dosagem , Etanidazol , Lomustina/administração & dosagem , Melfalan/administração & dosagem , Camundongos , Misonidazol/administração & dosagem , Nitroimidazóis/administração & dosagem , OxigênioAssuntos
Contagem de Leucócitos , Lítio/farmacologia , Irradiação Corporal Total , Animais , Feminino , Carbonato de Lítio , Ratos , Ratos EndogâmicosAssuntos
Neoplasias Experimentais/radioterapia , Radiossensibilizantes/uso terapêutico , Amifostina/uso terapêutico , Animais , Doenças do Sistema Nervoso Central/induzido quimicamente , Cães , Humanos , Hipertermia Induzida , Técnicas In Vitro , Intestinos/efeitos da radiação , Linfócitos/efeitos dos fármacos , Metronidazol/uso terapêutico , Camundongos , Misonidazol/uso terapêutico , Neoplasias/radioterapia , Neoplasias Experimentais/metabolismo , Tecido Nervoso/efeitos dos fármacos , Tecido Nervoso/efeitos da radiação , Nitrocompostos/uso terapêutico , Oxigênio/metabolismo , Doses de Radiação , Protetores contra Radiação/uso terapêutico , Radiossensibilizantes/efeitos adversos , Radiossensibilizantes/toxicidade , RatosRESUMO
In a survey the definition of the notions insufficiency of the bone marrow, panmyelopathy, panmyelophthisis and aplastic anaemia, the subdivision into congenital and acquired forms as well as to the clinical course, the epidemiologic situation as well as the distribution according to age and sex are described. More in detail the author enters the etiological factors, in which cases the etiological classification of the individual case may be connected with considerable difficulties. Exogenic noxae are subdivided into factors dependent on dose and independent on dose as pathogenetic principles. In this connection professional noxious substances as well as unwished side-effects of medicaments are discussed more in detail. Despite all variety the possibilities of therapy are limited.