An examination of the influence of drained peatlands on regional stream water chemistry.

Currently, 50% of Irish rivers do not meet water quality standards, with many declining due to numerous pressures, including peatland degradation. This study examines stream water quality in the Irish midlands, a region where raised bogs have been all historically disturbed to various extent and the majority drained for industrial or domestic peat extraction. For the first time, we provide in-depth analysis of stream water chemistry within a heavily modified bog landscape. Small streams from degraded bogs exhibited greater levels of pollutants, in particular: total dissolved nitrogen (0.48 mg/l) and sulphate (18.49 mg/l) as well as higher electrical conductivity (mean: 334 µS/cm) compared to similar bog streams in near-natural bogs. Except for site-specific nitrogen pollution in certain streams surrounding degraded peatlands, the chemical composition of the receiving streams did not significantly differ between near-natural and degraded sites, reflecting the spatio-temporal scales of disturbance in this complex peat-scape. Dissolved organic carbon concentrations in all the receiving streams were high (27.2 mg/l) compared to other Irish streams, even within other peatland catchments. The region is experiencing overall a widespread loss of fluvial nitrogen and carbon calling for (a) the development of management instruments at site-level (water treatment) and landscape-level (rewetting) to assist with meeting water quality standards in the region, and (b) the routine monitoring of water chemistry as part of current and future peatland management activities. Supplementary Information: The online version contains supplementary material available at 10.1007/s10750-023-05188-5.

Menstrual hygiene management practices and determinants among schoolgirls in Addis Ababa, Ethiopia: The urgency of tackling bottlenecks - Water and sanitation services.

Background: Menstrual hygiene management (MHM) service provision and improving schoolgirls' hygienic practices in schools are among the major challenges for low and middle income countries, including Ethiopia, in meeting the 2030 Sustainable Development Goals. This study was conducted to assess schoolgirls' MHM practices and what influences those practices in Addis Ababa, the capital city of Ethiopia. Methods: A cross-sectional study was conducted on 401 adolescent schoolgirls and 98 school directors that were selected using a multistage sampling method. Pretested semi-structured interviewer-administered questionnaires and observational checklists were used to collect data. Results: During menstruation, about 90% of schoolgirls used commercially made disposable sanitary pads. However, only 45.9% of girls had access to emergency pads from their schools. Of the 98 directors, 79 (80.6%) responded that they had MHM provisions for schoolgirls. However, 42 (42.9%) schools had no water and soap in the pad changing rooms/toilets, and 70% lacked a covered dustbin for disposal/storage of soiled sanitary pads. Besides, more than 55% of the schools practiced open burning and dumping to dispose of used menstrual materials. More than half of the schools had no sanitary pad changing rooms, three-quarters of them lack MHM education, and only 2.5% had a bathing area. The location of schools (AOR = 5.44, 95% CI: (2.34-12.66)), health club availability (AOR = 3.14, 95% CI: (1.53, 6.42)), being informed about MHM before menarche (AOR = 2.04, 95% CI: (1.04, 4.00)), and availability of emergency sanitary pad at schools (AOR = 2.59, 95% CI: (1.36, 4.91)) were significantly associated with the status of schoolgirls menstrual hygiene practices. Conclusions: About one-quarter of the schoolgirls had poor menstrual hygiene practices. Being a student in inner-city schools, attending a school that had a health club, being informed about MHM before menarche, and having access to emergency pads from schools were the determinant factors for good menstrual hygiene practices. However, most schools lack water, soap, and a covered dustbin in the pad changing rooms/toilets. Moreover, only a few schools provided MHM education and emergency pads. Improving water and sanitation services along with tailored MHM education are urgently needed to circumvent unsafe MHM practices among adolescent schoolgirls.

Nanopore metatranscriptomics reveals cryptic catfish species as potential Shigella flexneri vectors in Kenya.

Bacteria in the Shigella genus remain a major cause of dysentery in sub-Saharan Africa, and annually cause an estimated 600,000 deaths worldwide. Being spread by contaminated food and water, this study highlights how wild caught food, in the form of freshwater catfish, can act as vectors for Shigella flexneri in Southern Kenya. A metatranscriptomic approach was used to identify the presence of Shigella flexneri in the catfish which had been caught for consumption from the Galana river. The use of nanopore sequencing was shown to be a simple and effective method to highlight the presence of Shigella flexneri and could represent a potential new tool in the detection and prevention of this deadly pathogen. Rather than the presence/absence results of more traditional testing methods, the use of metatranscriptomics highlighted how primarily one SOS response gene was being transcribed, suggesting the bacteria may be dormant in the catfish. Additionally, COI sequencing of the vector catfish revealed they likely represent a cryptic species. Morphological assignment suggested the fish were widehead catfish Clarotes laticeps, which range across Africa, but the COI sequences from the Kenyan fish are distinctly different from C. laticeps sequenced in West Africa.

Using weighted expert judgement and nonlinear data analysis to improve Bayesian belief network models for riverine ecosystem services.

Rivers are a key part of the hydrological cycle and a vital conduit of water resources, but are under increasing threat from anthropogenic pressures. Linking pressures with ecosystem services is challenging because the processes interconnecting the physico-chemical, biological and socio-economic elements are usually captured using heterogenous methods. Our objectives were, firstly, to advance an existing proof-of-principle Bayesian belief network (BBN) model for integration of ecosystem services considerations into river management. We causally linked catchment stressors with ecosystem services using weighted evidence from an expert workshop (capturing confidence among expert groups), legislation and published literature. The BBN was calibrated with analyses of national monitoring data (including non-linear relationships and ecologically meaningful breakpoints) and expert judgement. We used a novel expected index of desirability to quantify the model outputs. Secondly, we applied the BBN to three case study catchments in Ireland to demonstrate the implications of changes in stressor levels for ecosystem services in different settings. Four out of the seven significant relationships in data analyses were non-linear, highlighting that non-linearity is common in ecosystems, but rarely considered in environmental modelling. Deficiency of riparian shading was identified as a prevalent and strong influence, which should be addressed to improve a broad range of societal benefits, particularly in the catchments where riparian shading is scarce. Sediment load had a lower influence on river biology in flashy rivers where it has less potential to settle out. Sediment interacted synergistically with organic matter and phosphate where these stressors were active; tackling these stressor pairs simultaneously can yield additional societal benefits compared to the sum of their individual influences, which highlights the value of integrated management. Our BBN model can be parametrised for other Irish catchments whereas elements of our approach, including the expected index of desirability, can be adapted globally.

A Bayesian Modelling Framework for Integration of Ecosystem Services into Freshwater Resources Management.

Models of ecological response to multiple stressors and of the consequences for ecosystem services (ES) delivery are scarce. This paper describes a methodology for constructing a BBN combining catchment and water quality model output, data, and expert knowledge that can support the integration of ES into water resources management. It proposes "small group" workshop methods for elucidating expert knowledge and analyses the areas of agreement and disagreement between experts. The model was developed for four selected ES and for assessing the consequences of management options relating to no-change, riparian management, and decreasing or increasing livestock numbers. Compared with no-change, riparian management and a decrease in livestock numbers improved the ES investigated to varying degrees. Sensitivity analysis of the expert information in the BBN showed the greatest disagreements between experts were mainly for low probability situations and thus had little impact on the results. Conversely, in our applications, the best agreement between experts tended to occur for the higher probability, more likely, situations. This has implications for the practical use of this type of model to support catchment management decisions. The complexity of the relationship between management measures, the water quality and ecological responses and resulting changes in ES must not be a barrier to making decisions in the present time. The interactions of multiple stressors further complicate the situation. However, management decisions typically relate to the overall character of solutions and not their detailed design, which can follow once the nature of the solution has been chosen, for example livestock management or riparian measures or both.

Refining benthic macroinvertebrate kick sampling protocol for wadeable rivers and streams in Ethiopia.

Streams and rivers cover a larger proportion of the Earth's surface but are highly affected by human pressures. Conversely, bioassessment methods are in their infancy in developing countries such as Ethiopia. In this study, we compared 2- and 3-min macroinvertebrate kick samples at multiple locations for both riffle habitat (RH) and multihabitat (MH) approaches. The performance of each method was evaluated statistically using benthic macroinvertebrate metrics and diversity indices. Results of the Kruskal-Wallis analysis in this study showed no significant differences among methods tested in minimally impacted streams in Ethiopia and generally performed equally irrespective of the methods employed except for total abundances and Ephemeroptera abundances. Furthermore, multivariate analysis of the relative abundances of macroinvertebrate communities using analysis of similarity (ANOSIM), RELATE, non-metric multidimensional scaling (MDS), and classification strength-sampling method comparability (CS-SMC) indicated a high similarity in the macroinvertebrate communities recorded among all methods employed in this study area. However, the index of multivariate dispersion (IMD) test showed variations in relative abundances of macroinvertebrate communities among the methods. In summary, if the focus is not on rare taxa and the required information is not dependent on additional evidence provided by the use of lower taxonomic levels of identification (genus and species), the results of the present study support the use of the shorter 2-min RH kick sampling method for the bioassessment of wadeable rivers and streams in Ethiopia.

Coarse sediment dynamics and low-head dams: Monitoring instantaneous bedload transport using a stationary RFID antenna.

Coarse sediment transport in fluvial systems serves an important role in determining in-stream physical habitat, spawning potential and benthic community structure. However, despite more than a decade of pressure in Europe to restore stream continuity under the Water Framework Directive (WFD), there have been relatively few empirical studies on how low-head, run-of-river structures (i.e., weirs) disrupt the processes and dynamics of bedload conveyance. In this study we present an investigation into how coarse sediment is transferred through a low-head dam via the real-time monitoring of bedload transport over a weir in southeast Ireland. Critical discharge values for particle entrainment over the structure were derived from the novel use of a stationary RFID antenna, coupled with continuous recording of water levels and sediment captured downstream using pit-style sediment traps. The stationary RFID antenna was installed along a weir crest using both 'pass-under' and 'pass-over' configurations as a means of detecting the moment bedload tracers moved over the dam crest. Results show that 10% of tracers deployed upstream were detected passing over the weir, while a further 15% that were not detected were recovered downstream. These results indicate bedload material as large as the upstream D70 (i.e., 90 mm) can move over the structure during infrequent high-flow events. However, thorough searches of the seeded area upstream of the dam also suggest that as many as 43% of the total number may have passed downstream, indicating that tracers moved over the weir after the antenna was damaged during a high-flow event, or were missed due to either particle velocity or signal collision. In addition, 30 of the tracers that remained upstream were shown to have either been buried due to the subsequent influx of sediment entering the reservoir, or were reworked though the surface material. Critical discharge values indicate size-selective transport patterns may dominate and a strong correlation between event peak discharge and total bedload captured downstream. These findings provide more evidence that low-head structures may eventually adopt a morphology that allows for the intermittent storage and later export of a channel's bedload downstream as hypothesized by other authors. Building upon these findings and those of other recent field studies, we present a set of possible schematic models that offer a basis for understanding the unique ways low-head dams can continue to disrupt sediment conveyance long after they have reached their functional storage capacity. The limitations of using a stationary RFID antenna and possible recommendations for future studies are discussed.

Effect of low-head dams on reach-scale suspended sediment dynamics in coarse-bedded streams.

River infrastructure is one of the primary threats to riverine ecosystems globally, altering hydromorphological processes and isolating habitats. Instream barriers and low-head dams can have significant effects on system connectivity, but despite this, very few empirical studies have assessed the impacts of these structures on suspended sediment transport. Through a paired turbidity study over a 20-month monitoring period we investigated the differences in suspended sediment flux above and below two low-head dams in the south-east of Ireland. Using sediment balance as a proxy for sediment storage, results showed that a net-export of sediment from the study reach occurred for 68% of the high-flow events analysed. As the primary controls on sediment dynamics at the downstream reach depend on sediment availability from upstream, we argue that these results indicate the presence of a substantial local source of sediment between monitoring stations that cannot be explained by natural intra-reach erosional processes. Here we hypothesise that as sediment supply from the catchment becomes exhausted, the structure's impounded zone (typically considered a depositional area) provides a major sediment source to the downstream reach. Our rationale is that if sediment trapped behind the weir is periodically available for transportation at the rates and frequencies observed in this study, then we can infer that both structures must be trapping sediment under lower flows.

An inspection-based assessment of obstacles to salmon, trout, eel and lamprey migration and river channel connectivity in Ireland.

Knowledge of the location, physical attributes and impacts of obstacles on river connectivity is a requirement for any mitigating action aimed at restoring the connectivity of a river system. Here, we present a study that recorded the numbers and physical diversity of obstacles in 10 river catchments in Ireland, together with the impact these structures had on overall river connectivity. A total of 372 obstacles were recorded, 3 of these were dams, and the remainder were low-head weirs/sluices, obstacles associated with road or rail crossings of rivers and natural structures. The degree of fragmentation was estimated in each catchment by calculating obstacle density and the Dendritic Connectivity Index (DCI). DCI scores were calculated for 4 native Irish fish species with different life-histories, namely diadromous (Atlantic salmon, sea trout, European eel, sea lamprey) and potamodromous (brown trout). Obstacle density ranged between 1.2 and 0.02 obstacles/km of river. Six of the 10 catchments had at least one obstacle located on the mainstem river at least 5 km from its mouth/confluence. These 6 catchments typically had the lowest connectivity scores for diadromous species and ranged between 0.6 and 44.1 (a fully connected river would receive a maximum score of 100). While there was no significant correlation between obstacle density and the DCI score for diadromous fish, a significant negative correlation was detected between obstacle density and the DCI score for potamodromous brown trout. Here, we highlight the merit of these obstacle assessments and associated challenges for decision-making relating to prioritisation of obstacles for removal or modification.

Impact of low-head dams on bedload transport rates in coarse-bedded streams.

This paper presents an empirical study that uses the movement of RFID tracers to investigate the impacts of low-head dams on solid transport dynamics in coarse-bedded streams. Here we report on the influence of two structures located in Ireland's South-East, both of which indicate that particles greater than the reach D90 can be carried through and over low-head dams. This observation suggests that both structures may have reached a state of 'transient storage' as hypothesized by previous research. However, when the data were reinterpreted as fractional transport rates using a novel application of existing empirical relations, we observed patterns consistent with supply-limited conditions downstream. Expanding on existing conceptual models and mechanisms, we illustrate how a system may continue to exhibit supply-limited conditions downstream without the need for a net attenuation of sediment to occur indefinitely. We propose that once a transient storage capacity has been reached, the system then enters a state of dynamic disconnectivity where the long-term average sediment flux equals that under reference conditions, but now with the amplitude and wavelength of these sediment fluctuations having increased. We hypothesize that the time-lag associated with the reduced frequency of events competent enough to move bedload over the structure accounts for the time necessary to complete the 'fill' phase of the transient storage dynamic; a process that will continue until both the fill and flow thresholds are again met to allow the system to reenter the 'scour' phase. This model reconciles how a system may exhibit a sediment deficit for time intervals longer than those experienced under reference conditions. As water and sediment are the drivers of channel morphology and associated habitat units, the impact a structure has on a channel's sediment regime should therefore form part of any assessment regarding the prioritization of barriers for removal or remediation.

Chronic nutrient inputs affect stream macroinvertebrate communities more than acute inputs: An experiment manipulating phosphorus, nitrogen and sediment.

Freshwaters worldwide are affected by multiple stressors. Timing of inputs and pathways of delivery can influence the impact stressors have on freshwater communities. In particular, effects of point versus diffuse nutrient inputs on stream macroinvertebrates are poorly understood. Point-source inputs tend to pose a chronic problem, whereas diffuse inputs tend to be acute with short concentration spikes. We manipulated three key agricultural stressors, phosphorus (ambient, chronic, acute), nitrogen (ambient, chronic, acute) and fine sediment (ambient, high), in 112 stream mesocosms (26 days colonisation, 18 days of manipulations) and determined the individual and combined effects of these stressors on stream macroinvertebrate communities (benthos and drift). Chronic nutrient treatments continuously received high concentrations of P and/or N. Acute channels received the same continuous enrichment, but concentrations were doubled during two 3-hour periods (day 6, day 13) to simulate acute nutrient inputs during rainstorms. Sediment was the most pervasive stressor in the benthos, reducing total macroinvertebrate abundance and richness, EPT (mayflies, stoneflies, caddisflies) abundance and richness. By contrast, N or P enrichment did not affect any of the six studied community-level metrics. In the drift assemblage, enrichment effects became more prevalent the longer the experiment went on. Sediment was the dominant driver of drift responses at the beginning of the experiment. After the first acute nutrient pulse, sediment remained the most influential stressor but its effects started to fade. After the second pulse, N became the dominant stressor. In general, impacts of either N or P on the drift were due to chronic exposure, with acute nutrient pulses having no additional effects. Overall, our findings imply that cost-effective management should focus on mitigating sediment inputs first and tackle chronic nutrient inputs second. Freshwater managers should also take into account the length of exposure to high nutrient concentrations, rather than merely the concentrations themselves.

The Environmental Impact of Cattle Access to Watercourses: A Review.

The degradation of freshwater resources and loss of freshwater biodiversity by anthropogenic activities, including agriculture, are of major global concern. Together with diffuse pollutants, point sources, such as where cattle have direct access to riparian margins and watercourses, can potentially present significant environmental challenges. These can include impacts on stream morphology, increased sedimentation, nutrient additions, microbial contamination, and impacts on aquatic biota. Mitigation measures aimed at reducing these frequently include reducing the amount of time cattle spend in riparian margins and watercourses. This is often accomplished through the provision of an alternative water supply and grazing management, or even cattle exclusion measures. Although a number of studies refer to potential negative impacts, there has been little attempt to review previous research on this topic. The key aim of this paper is to collate and review these disparate studies, as well as those relating to the effectiveness of mitigation measures. Although it is difficult to draw generalizations from studies due to the inherent variability between and within catchments, evidence pertaining to impacts in relation to sedimentation, pathogens, and riparian margin vegetation were strong. Conclusions in relation to impacts on stream morphology and nutrient parameters were less clear, whereas studies on responses of macroinvertebrate communities were particularly variable, with differences due to cattle access difficult to separate from catchment scale effects. A greater understanding of the impact of cattle access on watercourses under varying conditions will help inform policymakers on the cost effectiveness of existing management criteria and will help in revising existing measures.

Identifying spawning sites and other critical habitat in lotic systems using eDNA "snapshots": A case study using the sea lamprey Petromyzon marinus L.

Many aquatic species of conservation concern exist at low densities and are inherently difficult to detect or monitor using conventional methods. However, the introduction of environmental (e)DNA has recently transformed our ability to detect these species and enables effective deployment of limited conservation resources. Identifying areas for breeding, as well as the ecological distribution of species, is vital to the survival or recovery of a conservation species (i.e., areas of critical habitat). In many species, spawning events are associated with a higher relative abundance of DNA released within an aquatic system (i.e., gametes, skin cells etc.), making this the ideal time to monitor these species using eDNA techniques. This study aims to examine whether a "snapshot" eDNA sampling approach (i.e., samples taken at fixed points in chronological time) could reveal areas of critical habitat including spawning sites for our target species Petromyzon marinus. We utilized a species-specific qPCR assay to monitor spatial and temporal patterns in eDNA concentration within two river catchments in Ireland over three consecutive years. We found that eDNA concentration increased at the onset of observed spawning activity and patterns of concentration increased from downstream to upstream over time, suggesting dispersal into the higher reaches as the spawning season progressed. We found P. marinus to be present upstream of several potential barriers to migration, sometimes in significant numbers. Our results also show that the addition of a lamprey-specific fish pass at an "impassable" weir, although assisting in ascent, did not have any significant impact on eDNA concentration upstream after the pass had been installed. eDNA concentration was also found to be significantly correlated with both the number of fish and the number of nests encountered. The application of snapshot sampling techniques for species monitoring therefore has substantial potential for the management of low-density species in fast-moving aquatic systems.

Non-native species and lake warming negatively affect Arctic char Salvelinus alpinus abundance; deep thermal refugia facilitate co-existence.

This study finds that non-native species and warming temperatures have significant negative effects on Arctic char Salvelinus alpinus abundance in Irish lakes. Eutrophication was not important at the range of total phosphorus tested (0.005-0.023 mg l-1 ). Model results predict that S. alpinus occur across the temperature range sampled (8.2-19.7°C) when non-natives are absent, but S. alpinus catch is predicted to be close to zero irrespective of temperature when non-native catch is high. This result indicates that to persist, S. alpinus may require a habitat where non-natives are at low abundance or absent. Salvelinus alpinus segregated from other species along the thermal axis, inhabiting significantly colder water and actively avoided non-native species, which appeared to limit their distribution. The thermal niche realized by S. alpinus in non-native dominated lakes was thus compressed relative to native dominated lakes and S. alpinus population density was significantly lower. These findings were consistent even when the only non-native present was Perca fluviatilis. Temperature appeared to limit the distribution of non-native species, such that the presence of deep thermal refugia is currently facilitating S. alpinus co-existence with non-natives in associated lakes. Diet analysis identified P. fluviatilis as potential predators and competitors. This study provides strong evidence that non-native species are a key driver of low S. alpinus abundance in Irish lakes. Temperature increases associated with climate change are identified as a secondary concern, as they could erode S. alpinus' thermal niche and lead to their extirpation. The lower thermal buffering capacity of shallow lakes identifies these as higher risk systems. Salvelinus alpinus conservation in Ireland should focus on preventing future illegal non-native species introductions because unlike other stressors (e.g., eutrophication etc.), species introductions are rarely reversible.

Small Water Bodies in Great Britain and Ireland: Ecosystem function, human-generated degradation, and options for restorative action.

Small, 1st and 2nd-order, headwater streams and ponds play essential roles in providing natural flood control, trapping sediments and contaminants, retaining nutrients, and maintaining biological diversity, which extend into downstream reaches, lakes and estuaries. However, the large geographic extent and high connectivity of these small water bodies with the surrounding terrestrial ecosystem makes them particularly vulnerable to growing land-use pressures and environmental change. The greatest pressure on the physical processes in these waters has been their extension and modification for agricultural and forestry drainage, resulting in highly modified discharge and temperature regimes that have implications for flood and drought control further downstream. The extensive length of the small stream network exposes rivers to a wide range of inputs, including nutrients, pesticides, heavy metals, sediment and emerging contaminants. Small water bodies have also been affected by invasions of non-native species, which along with the physical and chemical pressures, have affected most groups of organisms with consequent implications for the wider biodiversity within the catchment. Reducing the impacts and restoring the natural ecosystem function of these water bodies requires a three-tiered approach based on: restoration of channel hydromorphological dynamics; restoration and management of the riparian zone; and management of activities in the wider catchment that have both point-source and diffuse impacts. Such activities are expensive and so emphasis must be placed on integrated programmes that provide multiple benefits. Practical options need to be promoted through legislative regulation, financial incentives, markets for resource services and voluntary codes and actions.

Multiple-stressor effects of sediment, phosphorus and nitrogen on stream macroinvertebrate communities.

Multiple stressors affect stream ecosystems worldwide and their interactions are of particular concern, with gaps existing in understanding stressor impacts on stream communities. Addressing these knowledge gaps will aid in targeting and designing of appropriate mitigation measures. In this study, the agricultural stressors fine sediment (ambient, low, medium, high), phosphorus (ambient, enriched) and nitrogen (ambient, enriched) were manipulated simultaneously in 64 streamside mesocosms to determine their individual and combined effects on the macroinvertebrate community (benthos and drift). Stressor levels were chosen to reflect those typically observed in European agricultural streams. A 21-day colonisation period was followed by a 14-day manipulative period. Results indicate that added sediment had the most pervasive effects, significantly reducing total macroinvertebrate abundance, total EPT abundance and abundances of three common EPT taxa. The greatest effect was at high sediment cover (90%), with decreasing negative impacts at medium (50%) and low (30%) covers. Added sediment also led to higher drift propensities for nine of the twelve drift variables. The effects of nitrogen and phosphorus were relatively weak compared to sediment. Several complex and unpredictable 2-way or 3-way interactions among stressors were observed. While sediment addition generally reduced total abundance at high levels, this decrease was amplified by P enrichment at low sediment, whereas the opposite effect occurred at medium sediment and little effect at high sediment. These results have direct implications for water management as they highlight the importance of managing sediment inputs while also considering the complex interactions which can occur between sediment and nutrient stressors.

The future of biotic indices in the ecogenomic era: Integrating (e)DNA metabarcoding in biological assessment of aquatic ecosystems.

The bioassessment of aquatic ecosystems is currently based on various biotic indices that use the occurrence and/or abundance of selected taxonomic groups to define ecological status. These conventional indices have some limitations, often related to difficulties in morphological identification of bioindicator taxa. Recent development of DNA barcoding and metabarcoding could potentially alleviate some of these limitations, by using DNA sequences instead of morphology to identify organisms and to characterize a given ecosystem. In this paper, we review the structure of conventional biotic indices, and we present the results of pilot metabarcoding studies using environmental DNA to infer biotic indices. We discuss the main advantages and pitfalls of metabarcoding approaches to assess parameters such as richness, abundance, taxonomic composition and species ecological values, to be used for calculation of biotic indices. We present some future developments to fully exploit the potential of metabarcoding data and improve the accuracy and precision of their analysis. We also propose some recommendations for the future integration of DNA metabarcoding to routine biomonitoring programs.

Pollution Assessment of Toxic and Potentially Toxic Elements in Agricultural Soils of the City Addis Ababa, Ethiopia.

Due to the significantly fast urban expansion and increased industrial activities, the soils in the farms in Addis Ababa are contaminated by some toxic and potentially toxic elements (As, V, Cr, Fe, Co, Ni, Cu, B, Ba, Sr, Zn, Mn, Pb and Cd) in varying degrees. The mean concentrations of Cr, Ni, As and B in most of the soil farms were found to be higher than the maximum recommended limits. The mean concentrations of Cd, Cu, Pb, Co, Ni and Mn were found to be higher than the background soil concentrations given for uncontaminated soils. Multivariate analyses coupled with correlation analysis were used to identify possible sources. The geo-accumulation index values for Cr, Mn and Pb indicated that the farm soils were unpolluted to moderately polluted as a result of anthropogenic activities. A comprehensive environmental management strategy should be formulated by the government to measure further pollution of the farmland soil.

A Water Framework Directive-compatible metric for assessing acidification in UK and Irish rivers using diatoms.

Freshwater acidification continues to be a major problem affecting large areas of Europe, and while there is evidence for chemical recovery, similar evidence for biological recovery of freshwaters is sparse. The need for a methodology to identify waterbodies impacted acidification and to assess the extent of biological recovery is relevant to the EU Water Framework Directive, which requires methods to quantify differences in biology between impacted and unimpacted or reference sites. This study presents a new WFD-compliant metric based on diatoms (Diatom Acidification Metric: DAM) for assessing the acidification status of rivers. A database of 558 benthic diatom samples and associated water chemistry data was assembled. Diatom taxa were assigned to one of 5 indicator classes on the basis of their pH optimum, assessed using Gaussian logistic regression, and these indicator values used to calculate a DAM score for each site using weighted averaging. Reference sites were selected on the basis of their acid neutralising capacity (ANC) and calcium concentration, and a regression model developed to predict expected DAM for each site using pH and total organic carbon (TOC) concentration. Site-specific DAM scores were used to calculate ecological quality ratios ranging from ≥1, where the diatom assemblage showed no impact, to (theoretically) 0, when the diatom assemblage was indicative of major anthropogenic activities. The boundary between 'high' and 'good' status was defined as the 25th percentile of Ecological Quality Ratios (EQRs) of all reference sites. The boundary between 'good' and 'moderate' status was set at the point at which nutrient-sensitive and nutrient-tolerant taxa were present in equal relative abundance. The methodology was evaluated using long-term data from 11 sites from the UK Uplands Waters Monitoring Network and is shown to perform well in discriminating naturally acid from acidified sites.

The effect of forest windrowing on physico-chemical water quality in Ireland.

Windrowing is widely practised, across Europe and North America, in bole-only harvested coniferous forest plantations before replanting. Forest harvesting has been shown to significantly increase sediment and nutrient losses to watercourses in other studies but windrowing effects, which are as bad, have not been investigated in detail. To determine physico-chemical impacts on water quality and to help inform forest managers, the effects of windrowing were investigated in a headwater catchment. Water samples were collected from storm events pre- (PWR), during (DWR) and after windrowing (AWR). Total suspended solids (TSS), total phosphorus (TP), soluble reactive phosphorus (SRP), total ammonia, nitrate, stream discharge, water level and velocity were measured. Results showed that peak and flow weighted mean concentrations (FWMC) of TSS concentrations increased significantly during windrowing when compared to pre-windrowing concentrations. Peak TSS increased from 88 mg/l (PWR) to 502 mg/l (DWR) and decreased to 163 mg/l (one year AWR) and 225 mg/l (two years AWR). Peak and FWMC of TP also increased during windrowing when compared to pre-windrowing concentrations. Peak TP concentrations increased from 0.1 mg/l (PWR) to 0.4 mg/l (DWR) and decreased to 0.1 mg/l (AWR). SRP and nitrate concentrations increased during windrowing when compared to pre-windrowing but remained low overall. TSS and TP concentrations were highest when flows greater than 0.3 m(3)/s (exceeded 6.3% of time) were recorded in the channel. It was highlighted that high-resolution sampling of storm events is important, where precise measurements of windrowing-sourced outputs are required. Windrowing was shown to generate very high concentrations of TSS and TP, comparable to those recorded during harvesting. This research helps to identify potential impacts on physico-chemical water quality that arise during windrowing and demonstrates the need for measures to minimize impacts on surface waters as required by the EU Water Framework Directive and similar legislation elsewhere.