Robotized access to the medullary cavity for intramedullary nailing of the femur.

INTRODUCTION: The insertion site for an antegrade femoral intramedullary nail in the treatment of a femoral shaft fracture has traditionally been performed using a free-hand technique. An inappropriate starting point can result in suboptimal nail insertion leading to malreduction, or iatrogenic fracture. Furthermore, repeated attempts to establish the optimal starting point can cause additional soft tissue trauma and radiation exposure. In the following study we compared a robot-guided technique with the standard free-hand technique for establishing the entry point of an antegrade femoral nail. We hypothesized that the robot-guided technique is more reliable and efficient. METHODS: A custom-made drill-guide was mounted onto the arm of an industrial robot. Two orthogonal fluoroscopic images were acquired from the proximal femur of five cadaveric human specimens. Images were processed with a special software in order to create an enhanced contour-recognition map from which the bone axes were automatically calculated. The drilling trajectory was computed along the extension of the bone-axis. The robot then moved the drill-guide on this trajectory toward the entry point. The drilling was then performed by the surgeon. In the control group, five cadaveric human femora were utilized to manually establish the starting point using the free-hand technique. RESULTS: 100% of the intramedullary cavities were successfully accessed with both the robot-guided and the manual techniques. In the manual technique repositioning of the drill was necessary in three out of five cases. The mean number of acquired fluoroscopic images was significantly reduced from 11.6 (manual) to 4 (robot-guided). CONCLUSION: Robot-assisted drilling of the entry-point in antegrade femoral nailing is more reliable and requires fewer radiographic images than the free hand technique. Yet, based on economical and logistical considerations, its application will probably only be accepted when a concomitant application for fracture reduction is available.

A biomechanical comparison of locked plate fixation with percutaneous insertion capability versus the angled blade plate in a subtrochanteric fracture gap model.

OBJECTIVES: The angled blade plate has been the historical standard in fixed-angle extramedullary subtrochanteric femur fracture fixation, but it requires an extensile lateral approach to the femur. Little formal evaluation exists for specifically designed percutaneous extramedullary implants. The purpose of this study was to compare 3 locked plating constructs, all with percutaneous insertion capability, with the standard 95-degree angled blade plate to determine whether specifically designed fixed-angle extramedullary implants for subtrochanteric femur fractures were biomechanically comparable to the angled blade plate. METHODS: Forty composite adult femurs were divided into 4 equal groups. The constructs evaluated included a 95-degree angled blade plate, a broad 4.5-mm combination locking plate, and a precontoured proximal femoral locking plate (PFLP) with and without an oblique, angled strut or "kickstand" screw. A 30-degree wedge osteotomy was used to create a subtrochanteric fracture gap model. Each specimen underwent axial and torsional stiffness testing along with cyclic axial loading to failure. RESULTS: Axial stiffness testing revealed that the PFLP with the "kickstand" screw was the stiffest construct (92.2 +/- 17.4 Nm/m), which was 211% stiffer than the blade plate, 309% stiffer than the broad plate, and 194% stiffer than the PFLP without the kickstand screw. The blade plate had the highest torsional stiffness (2.42 +/- 0.08 Nm/degree), which was 151% stiffer than the broad plate, 128% stiffer than the PFLP with the kickstand, and 138% stiffer than the PFLP without the kickstand screw. The PFLP with the kickstand screw had the least irreversible deformation (6.3 mm), which was 52% less than the broad plate and 61% less than the PFLP without the kickstand screw. CONCLUSIONS: Our data reveal that the PFLP with the "kickstand" screw provides more axial stiffness, less torsional stiffness, and equivalent irreversible deformation to cyclic axial loading when compared with the blade plate.

Comparative study of different intraoperative 3-D image intensifiers in orthopedic trauma care.

INTRODUCTION: Recently, isocentric C-arm fluoroscopy (Iso-C 3D) has been introduced as a precise imaging modality for intraoperative evaluation and management of fractures and osteosyntheses. The Siemens Iso-C 3D collects multiple fluoroscopic images during a 190-degree arc of rotation around the anatomic region of interest and reconstructs them into sagittal, axial, and coronal planes. Like the Iso-C 3D, the new Ziehm Vario 3D imaging system reconstructs images in multiple planes, but only requires a 136-degree arc of rotation. The purpose of this study was to compare the image quality and range of applicability of these two imaging systems. METHODS: All the tests were performed on a human cadaver. In the first part of the experiment, different bones and joints were scanned in their native condition using both the Iso-C 3D and Vario 3D. In the second part of the experiment, scans were performed in the same anatomic regions after simulated fractures and subsequent fixation. In some cases, suboptimal placement of hardware was intentionally undertaken. Direct visualization of the fracture construct and in certain cases computed tomographic (CT) imaging served as the gold standard. The scans from both imaging systems were analyzed using a DICOM viewer by five orthopedic trauma surgeons randomized and blinded to the study. The evaluation was based on the overall image quality, delineation of cancellous and cortical bone, delineation of joint surfaces, presence of artifacts, visualization quality of intra-articular incongruities, quality of reduction and implant positioning, and clinical applicability of the scan. These items were rated using a visual analog scale and a points system. A total of 55 3D scans were made and evaluated. RESULTS: There was no significant difference between the two imaging systems in terms of the overall image quality, delineation of cancellous and cortical bone, and the presence of artifacts. The delineation of joint surfaces was significantly better visualized with the Iso-C 3D. Furthermore, Iso-C 3D scans demonstrated a higher overall clinical applicability than Vario 3D images. However, the Vario 3D was able to provide superior quality with scans of the shoulder joint and the adipose tissue. There was no significant difference in the visualization of intra-articular incongruities, quality of reduction, and implant positioning. CONCLUSION: Although the Iso-C 3D imaging system was superior in delineating the joint surfaces, the image quality, and the overall clinical applicability, the study revealed that both devices provided 3D images with sufficient quality to the surgeon to assess clinically relevant questions, including the quality of fracture reduction and implant positioning. On the other hand, the Ziehm Vario 3D is capable of doing scans of the shoulder area, which could not be taken with the Siemens Iso-C 3D because of the isocentric design.

The accuracy and precision of computer assisted surgery in the assessment of frontal plane deviations of the lower extremity: a femoral fracture model.

OBJECTIVES: Mechanical axis deviation of the lower extremity as a result of malreduction or malunion of fractures plays an important role in the development of arthritis. Therefore it is crucial to restore the limb alignment as accurate as possible. The purpose of this study was to evaluate the accuracy and precision of navigation in assessing isolated frontal plane (varus/valgus) deviations of the lower limb in a simulated fracture model of the femur. MATERIALS AND METHODS: Three fracture models with ten specimens in each were created in femoral synthetic composite bones to simulate a subtrochanteric (AO/OTA 31-A1), mid-diaphyseal (AO/OTA 32-A3), and supracondylar (AO/OTA 33-A1) femur fracture. Each specimen was mounted on a custom holding device and registered with the navigation system. Eight custom-made aluminum wedges of varying angles (5 degrees -26 degrees ) were used to create varus/valgus angulations at the fracture site. After wedge placement, the frontal plane deformity was recorded and registered by the navigation system. The means and standard deviations for each navigated wedge angle were calculated and compared to the actual wedge angle using a one sample t test. A single factor ANOVA test was subsequently performed to see if the differences between the navigated mean angles in each fracture group were statistically significant. The level of significance was defined as P < 0.05. RESULTS: None of the navigated mean angles were found to be significantly different from the actual wedge angles (P = 0.05-1.00). More specifically, the differences between the navigated mean angles and the actual wedge angles ranged from 0 degrees to 0.7 degrees . Furthermore, the differences between the navigated mean angles in each angle group were found to be statistically insignificant (P = 0.53-0.99). CONCLUSION: The high accuracy and precision of navigation systems in determining frontal plane deformities of long bones can make them an invaluable tool for the exact reduction and realignment of lower extremity fractures.

Fixation of the femoral condyles: a mechanical comparison of small and large fragment screw fixation.

BACKGROUND: To compare the stability achieved using two 6.5-mm screws versus two or four 3.5-mm screws for the fixation of a unicondylar distal femur fracture. METHODS: A fracture model was created in femoral synthetic composite bones to simulate a lateral femoral condyle fracture (AO/OTA 33-B1). Fixation was performed using three different types of screw constructs: 1) two 6.5-mm cancellous screws inserted using the lag technique, 2) two 3.5-mm cortical screws inserted using the lag technique, and 3) four 3.5-mm cortical screws, with two inserted using the lag technique and two as position screws. After reduction and fixation, the constructs were axially loaded in a material-testing machine. Main outcome measurements were the mean load required to displace the osteotomy site 1 and 2 mm as well as the mean stiffness of the different fixation methods. RESULTS: The 6.5-mm construct required 56% more load to displace the osteotomy fragment 1 mm than the two 3.5-mm construct required (p < 0.0001), and 40% more load than the four 3.5-mm construct required (p < 0.0001). At loads that caused 2 mm of osteotomy displacement, these differences increased to 62% (p < 0.0001) and 48% (p < 0.0001), respectively. The mean loads needed to displace the osteotomy site were 28% higher for 1 mm of displacement (p = 0.003) and 27% higher for 2 mm of displacement (p = 0.03) for the four 3.5-mm screw construct compared with those needed for the two 3.5-mm group. The mean stiffness for the 6.5-mm group (1312.5 N/mm) was significantly higher than for the four 3.5-mm construct (784.2 N/mm; p < 0.0001) and the two 3.5-mm screw construct (409.4 N/mm; p < 0.0001). The difference in stiffness between the 3.5-mm groups was significant as well (p < 0.0001). CONCLUSION: Stabilization of a unicondylar distal femur fracture with two 6.5-mm cancellous screws provides the most rigid and stable fixation. If small fragment screws are used, a minimum of four 3.5-mm cortical screws should be used to approximate the mechanical stability of two 6.5-mm screws.

Mechanotransduction of bovine articular cartilage superficial zone protein by transforming growth factor beta signaling.

OBJECTIVE: Mechanical signals are key determinants in tissue morphogenesis, maintenance, and restoration strategies in regenerative medicine, although molecular mechanisms of mechanotransduction remain to be elucidated. This study was undertaken to investigate the mechanotransduction process of expression of superficial zone protein (SZP), a critical joint lubricant. METHODS: Regional expression of SZP was first quantified in cartilage obtained from the femoral condyles of immature bovines, using immunoblotting, and visualized by immunohistochemistry. Contact pressure mapping in whole joints was accomplished using pressure-sensitive film and a load application system for joint testing. Friction measurements on cartilage plugs were acquired under boundary lubrication conditions using a pin-on-disk tribometer modified for reciprocating sliding. Direct mechanical stimulation by shear loading of articular cartilage explants was performed with and without inhibition of transforming growth factor beta (TGFbeta) signaling, and SZP content in media was quantified by enzyme-linked immunosorbent assay. RESULTS: An unexpected pattern of SZP localization in knee cartilage was initially identified, with anterior regions exhibiting high levels of SZP expression. Regional SZP patterns were regulated by mechanical signals and correlated with tribological behavior. Direct relationships were demonstrated between high levels of SZP expression, maximum contact pressures, and low friction coefficients. Levels of SZP expression and accumulation were increased by applying shear stress, depending on location within the knee, and were decreased to control levels with the use of a specific inhibitor of TGFbeta receptor type I kinase and subsequent phospho-Smad2/3 activity. CONCLUSION: These findings indicate a new role for TGFbeta signaling in the mechanism of cellular mechanotransduction that is especially significant for joint lubrication.

Increased accumulation of superficial zone protein (SZP) in articular cartilage in response to bone morphogenetic protein-7 and growth factors.

The purpose of this study was to investigate the role of bone morphogenetic proteins (BMPs), such as BMP-7, growth factors, and cytokines, in the accumulation of superficial zone protein (SZP) in bovine articular cartilage. Calf superficial articular cartilage discs and chondrocytes were obtained for explant and monolayer culture systems, respectively. Dose- and time-dependent actions of BMP-7 on SZP accumulation were investigated in both explant and monolayer culture systems. In addition, actions of various morphogens and growth factors [BMP-2, BMP-4, fibroblast growth factor 2 (FGF-2), insulin-like growth factor 1 (IGF-1), platelet-derived growth factor (PDGF), and transforming growth factor beta (TGF-beta1)], and cytokines [interleukin (IL)-1alpha, IL-1beta, and tumor necrosis factor (TNF-alpha)] alone, and in combination with BMP-7, on SZP accumulation were investigated in monolayer culture systems. SZP accumulation was quantified in both the cartilage and the medium using SDS-PAGE and subsequent immunoblotting. In both explant and monolayer cultures, BMP-7 increased SZP accumulation in a dose- and time-dependent fashion (p < 0.05). Furthermore, SZP accumulation was significantly increased in monolayer cultures by FGF-2, IGF-1, PDGF, and TGF-beta1 (p < 0.05). Both IL-1alpha and TNF-alpha significantly reduced SZP accumulation (p < 0.05). The inhibition of SZP accumulation by TNF-alpha was partially alleviated by concurrent treatment with BMP-7. The results of this investigation provide novel insights into the role of morphogens, especially BMP-7, growth factors, and cytokines in the accumulation of SZP in articular cartilage. This information has clinical implications because stimulation of SZP may ameliorate the pathology of joint function in arthritis. Furthermore, tissue engineering approaches to articular cartilage may depend on the optimal synthesis and assembly of SZP in the superficial zone to ensure functional tissue architecture.

The effect of plate rotation on the stiffness of femoral LISS: a mechanical study.

OBJECTIVE: Malposition of the femoral Less Invasive Stabilization System (LISS) plate may alter its biomechanical behavior. This study compares the mechanical stability of "correctly" affixed LISS plates matching the slope of the lateral femoral condyle to "incorrectly" placed LISS plates fixed in external rotation relative to the distal femur. METHODS: A fracture gap model was created to simulate a comminuted supracondylar femur fracture (AO/OTA33-A3). Fixation was achieved using two different plate positions: the LISS plate was either placed "correctly" by internally rotating the plate to match the slope of the lateral femoral condyle, or "incorrectly" by externally rotating the plate relative to the distal femur. Following fixation, the constructs were loaded in axial, torsional, and cyclical axial modes in a material testing machine. MAIN OUTCOME MEASUREMENT: Stiffness in axial and torsional loading; total deformation and irreversible (plastic) deformation in cyclical axial loading. RESULTS: The mean axial stiffness for the correctly placed LISS constructs was 21.5% greater than the externally rotated LISS constructs (62.7 N/mm vs. 49.3 N/mm; P = 0.0007). No significant difference was found in torsional stiffness between the two groups. Cyclical axial loading caused significantly less (P < 0.0001) plastic deformation in the correct group (0.6 mm) compared with externally rotated group (1.3 mm). All the constructs in the incorrect group failed, where failure was defined as a complete closure of the medial fracture gap, prior to completion of the test cycles. CONCLUSION: Correct positioning of the LISS plate for fixation of distal femur fractures results in improved mechanical stability as reflected by an increased stiffness in axial loading and decreased plastic deformation at the bone-screw interface.

Plantar forefoot pressure changes after second metatarsal neck osteotomy.

BACKGROUND: The aim of this study was to evaluate plantar pressure changes after second metatarsal neck osteotomy using the Weil technique. METHODS: Six below-knee cadaver specimens were used. Each specimen was held in a custom-built apparatus and loaded to 500 N for a period of 3 seconds. Using a computerized Musgrave pedobarograph, pressure measurements were made before and after osteotomy in both neutral and 45-degree heel rise positions. All osteotomies were made at an angle of approximately 20 degrees relative to the long axis of the metatarsal shaft. The metatarsal heads were displaced proximally by 5 mm and fixed with a single Kirschner wire. RESULTS: After osteotomy there was an average decrease in pressure beneath the second metatarsal from 70.6 to 45.1 kPa in neutral and from 813.0 to 281.4 kPa in heel rise, representing statistically significant (p < or = 0.05) decreases of 36% and 65%, respectively. There also were significant decreases beneath the third metatarsal in both neutral (39%) and heel rise (37%), and beneath the fourth metatarsal in neutral position (28%). A significant pressure increase occurred beneath the first metatarsal in neutral (23%). No significant pressure changes occurred under the fifth metatarsal in either position. CONCLUSION: Overall, our results indicated that the Weil metatarsal neck osteotomy is effective at offloading the second metatarsal head at neutral and heel rise positions.

Effects of distraction on muscle length: mechanisms involved in sarcomerogenesis.

Although a great deal of interest has been given to understanding the mechanisms involved in regulating the radial growth that occurs because of resistance training, much less has been given to studying the longitudinal growth of skeletal muscle that occurs because of passive stretch. The current authors provide a brief overview of key issues relevant to the longitudinal growth of skeletal muscle that occurs during distraction osteogenesis. Specifically, five key issues are addressed: (1) the pattern of sarcomerogenesis during distraction; (2) sarcomerogenesis and altered expression of sarcomeric and nonsarcomeric genes; (3) the satellite cell hypothesis; (4) mitogenic factors; and (5) new approaches for studying the longitudinal growth of skeletal muscle. A discussion is provided that revolves around the concept of a negative feedback loop. One of the most interesting issues to be resolved in muscle biology is the role of satellite cells in regulating the growth of skeletal muscle. Currently, it is not known whether satellite cell activation is a prerequisite for the longitudinal growth of skeletal muscle. Gene chip analyses provide a paradoxical view, showing that distraction osteogenesis results in the upregulation of a gene, GADD45, involved with growth arrest and deoxyribonucleic acid destruction.