Potential of amino acids-modified biochar in mitigating the soil Cu and Ni stresses - Targeting the tomato growth, physiology and fruit quality.

Trace heavy metals (HMs) such as copper (Cu) and nickel (Ni) are toxic to plants, especially tomato at high levels. In this study, biochar (BC) was treated with amino acids (AA) to enhance amino functional groups, which effectively alleviated the adverse effects of heavy metals (HMs) on tomato growth. Hence, this study aimed to evaluate the effect of glycine and alanine modified BC (GBC/ABC) on various tomato growth parameters, its physiology, fruit yield and Cu/Ni uptake under Cu and Ni stresses. In a pot experiment, there was 21 treatments with three replications having two rates of simple BC and glycine/alanine enriched BC (0.5% and 1% (w/w). Cu and Ni stresses were added at 150 mg kg-1 respectively. Plants were harvested after 120 days of sowing and subjected to various analysis. Under Cu and Ni stresses, tomato roots accumulated more Cu and Ni than shoots and fruits, while GBC and ABC application significantly enhanced the root and shoot dry weight irrelevant to the stress conditions. Both rates of GBC decreased the malondialdehyde and hydrogen peroxide levels in plants. The addition of 0.5% GBC with Cu enhanced the tomato fruit dry weight by 1.3 folds in comparison to the control treatment; while tomato fruit juice content also increased (50%) in the presence of 0.5% GBC with Ni as compared to control. In summary, these results demonstrated that lower rate of GBC∼0.5% proved to be the best in mitigating the Cu and Ni stress on tomato plant growth by enhancing the fruit production.

Silicon-nanoparticles loaded biochar for soil arsenic immobilization and alleviation of phytotoxicity in barley: Implications for human health risk.

Arsenic (As)-induced environmental pollution and associated health risks are recognized on a global level. Here the impact of cotton shells derived biochar (BC) and silicon-nanoparticles loaded biochar (nano-Si-BC) was explored on soil As immobilization and its phytotoxicity in barley plants in a greenhouse study. The barley plants were grown in a sandy loam soil with varying concentrations of BC and nano-Si-BC (0, 1, and 2%), along with different levels of As (0, 5, 10, and 20 mg kg-1). The FTIR spectroscopy, SEM-EDX, and XRD were used to characterize BC and nano-Si-BC. Results revealed that As treatment had a negative impact on barley plant development, grain yield, physiology, and anti-oxidative response. However, the addition of nano-Si-BC led to a 71% reduction in shoot As concentration compared to the control with 20 mg kg-1 of As, while BC alone resulted in a 51% decline. Furthermore, the 2% nano-Si-BC increased grain yield by 94% compared to control and 28% compared to BC. The addition of 2% nano-Si-BC to As-contaminated soil reduced oxidative stress (34% H2O2 and 48% MDA content) and enhanced plant As tolerance (92% peroxidase and 46% Ascorbate peroxidase activity). The chlorophyll concentration in barley plants decreased due to oxidative stress. Additionally, the incorporation of 2% nano-Si-BC resulted in a 76% reduction in water soluble and NaHCO3 extractable As. It is concluded that the use of BC or nano-Si-BC in As contaminated soil for barley resulted in a low human health risk (HQ < 1), as it effectively immobilized As and promoted higher activity of antioxidants.

Adsorption of trace heavy metals through organic compounds enriched biochar using isotherm adsorption and kinetic models.

Trace heavy metals such as copper and nickel, when exceeds a certain level, cause detrimental effects on the ecosystem. The current study examined the potential of organic compounds enriched rice husk biochar (OCEB's) to remove the trace heavy metals from an aqueous solution in four steps. In 1st step, biochar' physical and chemical properties were analyzed through scanning electron microscope (SEM) and Fourier transforms infrared spectroscopy (FTIR). In the 2nd step, two biochar vis-a-vis glycine, alanine enriched biochar (GBC, ABC) was selected based on their adsorption capacity of four different metals Cr, Cu, Ni and Pb (chromium, copper, nickel, and lead). These two adsorbents (GBC, ABC) were further used to evaluate the best interaction of biochar for metal immobilization based on varying concentrations and times. Langmuir isotherm model suggested that the adsorption of Ni and Cu on the adsorbent surface supported the monolayer sorption. The qmax value of GBC for Cu removal increased by 90% compared to SBC (Simple rice husk biochar). The interaction of Cu and Ni with GBC and ABC was chemical, and 10 different time intervals were studied using pseud first and second-order kinetics models. The current study has supported the pseudo second-order kinetic model, which exhibited that the sorption of Ni and Cu occurred due to the chemical processes. The % removal efficiency with GBC was enhanced by 21% and 30% for Cu and Ni, respectively compared to the SBC. It was also noticed that GBC was 21% more efficient for % removal efficiency than the CBC. The study's findings supported that organic compound enriched rice husk biochar (GBC and ABC) is better than SBC for immobilizing the trace heavy metals from an aqueous solution.

Barriers and enablers to achieving clinical procedure competency-based outcomes in a national paediatric training/residency program-a multi-centered qualitative study.

BACKGROUND: In 2018, the Royal College of Physicians of Ireland revised its paediatric training program to a competency-based medical education (CBME) training/residency curriculum. This included a requirement to achieve competence in a number of core procedural skills to progress within the program. Internationally, simulation-based medical education (SBME) is gaining interest as an effective teaching pedagogy for training procedural skill competency. The objectives of this study were to (1) identify enablers and barriers for paediatric trainees to achieve their required procedural competencies, (2) gain insight on the feasibility of achieving the required procedural skills, and (3) explore what simulation-based resources are used as well as their role in achieving the required procedural skill competencies. METHODS: A multi-centered qualitative study using semi-structured interviews was performed. Twenty-four paediatric consultants and trainees were recruited from two academic tertiary hospitals using purposive and snowball sampling. Interviews were conducted between March and September 2021, audio recorded, transcribed, and analyzed using thematic analysis. RESULTS: Three main themes regarding enablers for achieving procedural competencies were reported and include having protected training time, routine assessments, and a standardized curriculum. Barriers to achieving procedural competencies focused mainly on limited clinical exposure. The use of SBME was recommended by all participants (n = 24, 100%) to assist in achieving procedural competencies and most (n = 15, 62.5%) reported it is feasible to attain the required procedural skills in the paediatric CBME program. CONCLUSION: It is feasible to achieve the required procedural competencies for most paediatric trainees, but this can be improved with protected training time, routine assessments, and a standardized curriculum. Barriers to achieving these skills mainly center on limited clinical exposure, which can be remedied by SBME. Further research is warranted to determine the costs and types of SBME tools available as well as teaching pedagogies to support paediatric trainees achieve their required procedural competencies.

Alleviation of chromium toxicity in mung bean (Vigna radiata L.) using salicylic acid and Azospirillum brasilense.

BACKGROUND: Chromium (Cr) contamination in soil poses a serious hazard because it hinders plant growth, which eventually reduces crop yield and raises the possibility of a food shortage. Cr's harmful effects interfere with crucial plant functions like photosynthesis and respiration, reducing energy output, causing oxidative stress, and interfering with nutrient intake. In this study, the negative effects of Cr on mung beans are examined, as well as investigate the effectiveness of Azospirillum brasilense and salicylic acid in reducing Cr-induced stress. RESULTS: We investigated how different Cr levels (200, 300, and 400 mg/kg soil) affected the growth of mung bean seedlings with the use of Azospirillum brasilense and salicylic acid. Experiment was conducted with randomized complete block design with 13 treatments having three replications. Significant growth retardation was caused by Cr, as were important factors like shoot and root length, plant height, dry weight, and chlorophyll content significantly reduced. 37.15% plant height, 71.85% root length, 57.09% chlorophyll contents, 82.34% crop growth rate was decreased when Cr toxicity was @ 50 µM but this decrease was remain 27.80%, 44.70%, 38.97% and 63.42%, respectively when applied A. brasilense and Salicylic acid in combine form. Use of Azospirillum brasilense and salicylic acid significantly increased mung bean seedling growth (49%) and contributed to reducing the toxic effect of Cr stress (34% and 14% in plant height, respectively) due to their beneficial properties in promoting plant growth. CONCLUSIONS: Mung bean seedlings are severely damaged by Cr contamination, which limits their growth and physiological characteristics. Using Azospirillum brasilense and salicylic acid together appears to be a viable way to combat stress brought on by Cr and promote general plant growth. Greater nutrient intake, increased antioxidant enzyme activity, and greater root growth are examples of synergistic effects. This strategy has the ability to reduce oxidative stress brought on by chromium, enhancing plant resistance to adverse circumstances. The study offers new perspectives on sustainable practices that hold potential for increasing agricultural output and guaranteeing food security.

Impact of psychotropic medications on cognition among older adults: a systematic review.

OBJECTIVES: The aim of this systematic review is to examine the cognitive impact of psychotropic medications including benzodiazepines, antidepressants, mood stabilizers, antipsychotics, or a combination of these drugs on older adults. DESIGN: Systematic review. SETTING: We searched Medline, PsycINFO, and Embase through the Ovid platform, CINAHL through EBSCO, and Web of Science. PARTICIPANTS AND INTERVENTIONS: Randomized control trials (RCTs) and cohort studies that used a validated scale to measure cognition with a follow-up period of at least six months were included. MEASUREMENT: The primary outcome of interest was cognitive change associated with psychotropic medication use. RESULTS: A total of 7551 articles were identified from the primary electronic literature search across the five databases after eliminating duplicates. Based on full-text analysis, 27 articles (two RCTs, 25 cohorts) met the inclusion criteria. Of these, nine each examined the impact of benzodiazepines and antidepressants, five examined psychotropic combinations, three on antipsychotic drugs, and one on the effects of mood stabilizers. CONCLUSIONS: This is the first systematic review to examine the cognitive impact of multiple psychotropic drug classes in older adults over an extended follow-up period (six months or more) using robust sample sizes, drug-free control groups, and validated cognitive instruments. We found evidence to indicate cognitive decline with the cumulative use of benzodiazepines and the use of antidepressants, especially those with anticholinergic properties among older adults without cognitive impairment at baseline. Further, the use of antipsychotics and psychotropic combinations is also associated with cognitive decline in older adults.

Does green HRM really matter for sustainable performance? The role of environmental consciousness and green intellectual capital.

The purpose of this study is to investigate the impact of a set of green human resource management (HRM) practices on sustainable performance in Pakistani higher education institutions (HEIs), while also taking into account the mediating influence of environmental consciousness and green intellectual capital. Furthermore, the study aims to assess the association between environmental consciousness and green intellectual capital, along with the sustainable outcome. The study data was collected from 250 HR managers and executive officers who were responsible for implementing green HRM practices and sustainable performance in the education sector of Pakistan. Smart PLS-4 software was used to perform the statistical analysis of the data. According to the results of this study, green HRM practices play a substantial role in enhancing sustainable performance. The study also identified a link between green HRM practices and sustainable performance via environmental awareness and green intellectual capital. The research contributes to the theoretical paradigm's social cognitive theory by offering information on green HRM practice bundles and sustainable performance. The research also demonstrates that green intellectual capital and environmental consciousness operate as a bridge between green HRM practices and long-term sustainable performance. The study's findings have real-world applications for education, policymakers, and human resource managers at the highest levels. In order to achieve sustainable performance, the study emphasizes the significance of developing green intellectual capital and implementing green HRM practices.

Effect of hydrophilic polymers on the solubility and dissolution enhancement of rivaroxaban/beta-cyclodextrin inclusion complexes.

BCS class II drugs exhibit low aqueous solubility and high permeability. Such drugs often have an incomplete or erratic absorption profile. This study aimed to predict the effects of ß-cyclodextrin (ßCD) and different hydrophilic polymers (poloxamer 188 (PXM-188), polyvinyl pyrrolidone (PVP) and soluplus (SOLO)) on the saturated solubility and dissolution profile of hydrophobic model drug rivaroxaban (RIV). Binary inclusion complex with ßCD were prepared by kneading and solvent evaporation method, at drug to cyclodextrin weight molar ratios of 1:1, 1:2, and 1:4. Saturated solubility of the hydrophobic model moiety was evaluated with ßCD to explore the increment in saturated solubility. Dissolution test was carried out to assess the drug release from the produced binary inclusion complex in the aqueous medium. Solid state analysis was performed using Fourier transform infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Scanning electron microscopy (SEM) techniques. When compared to pure drug, the binary complex (Drug: ßCD at molar ratio of 1:2 w/w) demonstrated the best performance in terms of enhanced solubility and drug release. Furthermore, ternary inclusion complex was prepared with hydrophilic polymers SOLO, PVP K-30 and PXM-188 at 0.5%,1%,2.5%,5% and 10% w/w to optimized binary formulation RIV:ßCD (1:2) prepared by kneading (KN) and solvent evaporation (S.E) method. The findings demonstrated that among ternary formulations (1:2 Drug: ßCD: SOLO 10% S.E) manifested greatest improvement in saturated solubility and dissolution rate. Results of solubility enhancement and improvement in dissolution profile of model drug by ternary inclusion complexation were also supported by FTIR, DSC, XRD, and SEM analysis. So, it can be concluded that the ternary inclusion systems were more effective compared to the binary combinations in improving solubility as well as dissolution of hydrophobic model drug rivaroxaban.

High morbidity and mortality in children with untreated congenital deficiency of leptin or its receptor.

The long-term clinical outcomes of severe obesity due to leptin signaling deficiency are unknown. We carry out a retrospective cross-sectional investigation of a large cohort of children with leptin (LEP), LEP receptor (LEPR), or melanocortin 4 receptor (MC4R) deficiency (n = 145) to evaluate the progression of the disease. The affected individuals undergo physical, clinical, and metabolic evaluations. We report a very high mortality in children with LEP (26%) or LEPR deficiency (9%), mainly due to severe pulmonary and gastrointestinal infections. In addition, 40% of surviving children with LEP or LEPR deficiency experience life-threatening episodes of lung or gastrointestinal infections. Although precision drugs are currently available for LEP and LEPR deficiencies, as yet, they are not accessible in Pakistan. An appreciation of the severe impact of LEP or LEPR deficiency on morbidity and early mortality, educational attainment, and the attendant stigmatization should spur efforts to deliver the available life-saving drugs to these children as a matter of urgency.

Exploring the Impact of Salicylic Acid and Farmyard Manure on Soil Rhizospheric Properties and Cadmium Stress Alleviation in Maize (Zea mays L.).

Cadmium (Cd) pollution is a growing environmental problem that negatively impacts plant growth and development, particularly in maize. In this research, the impact of farmyard manure (FYM) and salicylic acid (SA) on rhizospheric characteristics and the reduction of Cd stress in maize was examined at Government College (GC) University, Lahore, in 2022. The experiment was arranged with a randomized design, including three replications of 12 treatments (T1 = Control; T2 = Farmyard manure; T3 = Salicylic Acid; T4 = 100 mg/kg of soil Cd; T5 = 200 mg/kg of soil Cd; T6 = Farmyard manure + Salicylic acid; T7 = FYM + 100 mg/kg soil Cd; T8 = FYM + 200 mg/kg soil Cd; T9 = SA + 100 mg/kg soil Cd; T10 = SA + 200 mg/kg soil Cd; T11 = FYM + SA + 100 mg/kg soil Cd; T12 = FYM + SA + 200 mg/kg soil Cd). Results demonstrated that Cd stress negatively affected the maize plant and soil properties, but the application of SA and FYM was effective to mitigate the Cd stress up to a certain level. A reduction of 41.52%, 39.14%, and 39.94% in root length, length of the leaf, and crop growth rate was noticed, due to the Cd stress at 200 mg/kg soil, but this reduction was reduced to 18.83%, 10.35%, and 12.26%, respectively, when FYM and salicylic acid were applied as a combined application under the same stress level of Cd. The root biomass, leaf surface area, and length were all improved by SA and FYM, which enhanced the plant's capacity to absorb nutrients and improve growth under Cd stress. In conclusion, the use of salicylic acid together with farm manure can be an effective approach to mitigate Cd stress in maize crops.

Microplastics: a review of their impacts on different life forms and their removal methods.

The pollution of microplastics (MPs) is a worldwide major concern, as they have become a major part of our food chain. MPs enter our ecosystem via different pathways, including anthropogenic activities and improper disposal of plastics. The aim of this article is to review the current scientific literature related to MPs and how they affect different life forms on earth. Briefly, MPs induced negative effects on humans are primarily linked with the oxidative stress and disruption in immunity. MPs not only affect the soil chemical and physical properties such as reduction in soil health and productivity but also impose harmful effects on soil microorganisms. Moreover, MP-induced plant growth reduction results from three complementary mechanisms: (i) reduction in root and shoot growth, (ii) reduction in photosynthesis accompanied by higher reactive oxygen species (ROS) production, and (iii) reduction in nutrient uptake via altered root growth. Given the negative effects of MPs on different life forms, it is important to remove or remediate them. We have discussed different MP removal methods including coagulation, membrane filtration technology, biochar, and biological degradation of MPs in soil and wastewater effluents. The use of ozone as ultrafiltration technique has also been shown as the most promising technique for MP removal. Finally, some future research recommendations are also put forward at the end to further enhance our understanding of the MPs induced negative effects on different life forms. The flowchart shows the interaction of MPs from water contaminated with MPs with different parts of the ecosystem and final interaction with human health.

Prevalence and antimicrobial susceptibility of Acinetobacter spp. in a tertiary care hospital in Peshawar: a cross-sectional study.

Acinetobacter spp. have been a primary cause of nosocomial infections worldwide, causing significant morbidity and mortality, especially in Pakistan. The purpose of this study was to investigate the trend of antimicrobial resistance over a 5-year period in a tertiary care hospital in Pakistan. Methods: A retrospective cross-sectional study regarding the occurrence and antimicrobial resistance of Acinetobacter spp. recovered from clinical specimens that were referred to the Pathology Laboratory of Northwest General Hospital, Peshawar. The data from 2014 to 2019 was recorded and analyzed by the laboratory. Sociodemographic characteristics and laboratory record data was analyzed using SPSS, version 25. A chi-square test was applied to see the significance. Results: Of 59 483 clinical samples, Acinetobacter baumannii strains were detected in 114 of them. The majority of the clinical samples were from blood (89.5%) followed by sputum (7.9%), wound swab (1.8%), and bone marrow (0.9%). A. baumannii has been found in 52 men (67.53%) and 28 women (75.67%), with an overall risk of 0.669 times. In 76 men (98.70%), sensitivity for ertapenem (99.1), colistin (96.49), and tigecycline (78.9%) were also observed which indicated the potential viability of these drugs to treat multidrug-resistant (MDR) Acinetobacter infections. The male-to-female risk ratio was 0.98 for colistin and 0.71 for amikacin. Conclusion: Increased frequency of MDR supports the need for continuous surveillance to determine the prevalence and evolution of MDR Acinetobacter spp. in Pakistan. Colistin, tigecyclines, and ertapenem remain the possible line of drugs to treat MDR Acinetobacter.

A "Do No Harm" Novel Safety Checklist and Research Approach to Determine Whether to Launch an Artificial Intelligence-Based Medical Technology: Introducing the Biological-Psychological, Economic, and Social (BPES) Framework.

Given the impact artificial intelligence (AI)-based medical technologies (hardware devices, software programs, and mobile apps) can have on society, debates regarding the principles behind their development and deployment are emerging. Using the biopsychosocial model applied in psychiatry and other fields of medicine as our foundation, we propose a novel 3-step framework to guide industry developers of AI-based medical tools as well as health care regulatory agencies on how to decide if a product should be launched-a "Go or No-Go" approach. More specifically, our novel framework places stakeholders' (patients, health care professionals, industry, and government institutions) safety at its core by asking developers to demonstrate the biological-psychological (impact on physical and mental health), economic, and social value of their AI tool before it is launched. We also introduce a novel cost-effective, time-sensitive, and safety-oriented mixed quantitative and qualitative clinical phased trial approach to help industry and government health care regulatory agencies test and deliberate on whether to launch these AI-based medical technologies. To our knowledge, our biological-psychological, economic, and social (BPES) framework and mixed method phased trial approach are the first to place the Hippocratic Oath of "Do No Harm" at the center of developers', implementers', regulators', and users' mindsets when determining whether an AI-based medical technology is safe to launch. Moreover, as the welfare of AI users and developers becomes a greater concern, our framework's novel safety feature will allow it to complement existing and future AI reporting guidelines.

Biallelic Mutations in P4HTM Cause Syndromic Obesity.

We previously demonstrated that 50% of children with obesity from consanguineous families from Pakistan carry pathogenic variants in known monogenic obesity genes. Here, we have discovered a novel monogenetic recessive form of severe childhood obesity using an in-house computational staged approach. The analysis included whole-exome sequencing data of 366 children with severe obesity, 1,000 individuals of the Pakistan Risk of Myocardial Infarction Study (PROMIS) study, and 200,000 participants of the UK Biobank to prioritize genes harboring rare homozygous variants with putative effect on human obesity. We identified five rare or novel homozygous missense mutations predicted deleterious in five consanguineous families in P4HTM encoding prolyl 4-hydroxylase transmembrane (P4H-TM). We further found two additional homozygous missense mutations in children with severe obesity of Indian and Moroccan origin. Molecular dynamics simulation suggested that these mutations destabilized the active conformation of the substrate binding domain. Most carriers also presented with hypotonia, cognitive impairment, and/or developmental delay. Three of the five probands died of pneumonia during the first 2 years of the follow-up. P4HTM deficiency is a novel form of syndromic obesity, affecting 1.5% of our children with obesity associated with high mortality. P4H-TM is a hypoxia-inducible factor that is necessary for survival and adaptation under oxygen deprivation, but the role of this pathway in energy homeostasis and obesity pathophysiology remains to be elucidated.

Trichoderma asperellum L. Coupled the Effects of Biochar to Enhance the Growth and Physiology of Contrasting Maize Cultivars under Copper and Nickel Stresses.

Crop cultivation in heavy metal (HM)-polluted soils is a routine practice in developing countries that causes multiple human health consequences. Hence, two independent studies have been performed to investigate the efficiency of rice husk biochar (BC) and three fungal species, Trichoderma harzianum (F1), Trichoderma asperellum (F2) and Trichoderma viride (F3), to improve the growth and physiology of Zea mays L. plants grown on soil contaminated with Cu and Ni. Initially, a biosorption trial was conducted to test the HM removal efficiency of species F1, F2 and F3. Among them, F2 sp. showed the maximum Cu and Ni removal efficiency. Then, a pot study was conducted with two cultivars (spring corn and footer corn) having eleven treatments with three replicates. The results demonstrated a significant genotypic variation among both cultivars under applied HM stress. The maximum decreases in leaf Chl a. (53%), Chl b. (84%) and protein (63%) were reported in footer corn with applied Cu stress. The combined application of biochar and F2 increased leaf CAT (96%) in spring corn relative to Cu stress. Altogether, it was found that BC + F2 treatment showed the maximum efficiency in combatting Cu and Ni stress in spring corn.

Green HRM, green innovation and environmental performance: the role of green transformational leadership and green corporate social responsibility.

The current study attempted to inspect the influence of green human resource management (GHRM) practices and green innovation (GI) on environmental performance. Besides, the study considered green corporate social responsibility (GCSR) as a mediator to elaborate on the influence of GHRM and green innovation on environmental performance. Additionally, the current study assessed the role of green transformational leadership (GTFL) by the focus on GHRM and GI on GCSR. Data were gathered from 310 employees who are working in public and private banks by using a survey questionnaire. Subsequently, the data were analysed by using the partial least square structural equation modelling technique. The study findings showed that GHRM and GI positively influenced GCSR. In addition, the results revealed an insignificant relationship between GHRM and environmental performance (EP), whereas the influence of GI on environmental performance was significant. Moreover, GCSR positively influenced environmental performance. The results supported the mediator task of GCSR between the influence of GHRM and GI on EP. Finally, the findings indicated GTFL as a significant moderator. The study was theoretically grounded on NRBV theory. The study adds to the GHRM, GTFL, GCSR, green innovation and environmental performance theory in novel ways. The study also added to the literature by providing evidence on how transformational leadership can serve as a booster to transform the influence of GHRM on GCSR.

Antineutrophil Cytoplasmic Autoantibody-Associated Glomerulonephritis as a Possible Side Effect of COVID-19 Vaccination.

Vaccination is the principal tool aimed at curbing the COVID-19 pandemic that has, so far, affected tens of millions of individuals in the United States. The two available mRNA vaccines developed by Pfizer-BioNTech and Moderna possess high efficacy in preventing infection and illness severity. However, there are multiple side effects associated with these vaccines, some impacting different organs. Renal pathology is variable, with increasing cases of glomerulonephritis being observed. We report a rare acute kidney injury case due to antineutrophil cytoplasmic autoantibody (ANCA)-mediated glomerulonephritis after administering a second dose of the Pfizer-BioNTech mRNA SARS-CoV-2 vaccine. Aggravation and/or development of autoimmunity after mRNA vaccination may involve multiple immune mechanisms leading to de novo and recurrent glomerular diseases with an autoimmune basis.

Investigating the role of bentonite clay with different soil amendments to minimize the bioaccumulation of heavy metals in Solanum melongena L. under the irrigation of tannery wastewater.

Wastewater from tanneries is a major source of heavy metals in soil and plants when used for crop irrigation. The unavoidable toxicological effects of this contamination, however, can be minimized through two independent steps discussed in the present study. In the first step, a batch sorption experiment was conducted in which Cr was adsorbed through bentonite clay. For this purpose, DTPA extraction method was used to analyze Cr concentration in the soil after regular time intervals (0.5, 1, 2, 6, 8, 9, 10.5, 11.5, and 20.3 h) which reduced Cr concentration from 38.542 mgL-1 for 30 min to 5.6597 mgL-1 for 20.3 h, respectively, by applying 1% bentonite. An increase in the contact time efficiently allowed soil adsorbent to adsorb maximum Cr from soil samples. In the second step, a pot experiment was conducted with 10 different treatments to improve the physiological and biochemical parameters of the Solanum melongena L. irrigated under tanneries' wastewater stress. There were four replicates, and the crop was harvested after 30 days of germination. It was seen that the application of wastewater significantly (P < 0.01) reduced growth of Solanum melongena L. by reducing root (77%) and shoot (63%) fresh weight when compared with CFOP (Ce-doped Fe2O3 nanoparticles); chlorophyll a and b (fourfolds) were improved under CFOP application relative to control (CN). However, the deleterious effects of Cr (86%) and Pb (90%) were significantly decreased in shoot through CFOP application relative to CN. Moreover, oxidative damage induced by the tannery's wastewater stress (P < 0.01) was tolerated by applying different soil amendments. However, results were well pronounced with the application of CFOP which competitively decreased the concentrations of MDA (95%), H2O2 (89%), and CMP (85%) by efficiently triggering the activities of antioxidant defense mechanisms such as APX (threefold), CAT (twofold), and phenolics (75%) in stem relative to CN. Consequently, all the applied amendments (BN, BT, FOP, and CFOP) have shown the ability to efficiently tolerate the tannery's wastewater stress; results were more pronounced with the addition of CFOP and FOP+BT by improving physiological and biochemical parameters of Solanum melongena L. in an eco-friendly way.

Microbial-assisted soil chromium immobilization through zinc and iron-enriched rice husk biochar.

Soil chromium toxicity usually caused by the tannery effluent compromises the environment and causes serious health hazards. The microbial role in strengthening biochar for its soil chromium immobilization remains largely unknown. Hence, this study evaluated the effectiveness of zinc and iron-enriched rice husk biochar (ZnBC and FeBC) with microbial combinations to facilitate the chromium immobilization in sandy loam soil. We performed morphological and molecular characterization of fungal [Trichoderma harzianum (F1), Trichoderma viride (F2)] and bacterial [Pseudomonas fluorescence (B1), Bacillus subtilis (B2)] species before their application as soil ameliorants. There were twenty-five treatments having ZnBC and FeBC @ 1.5 and 3% inoculated with bacterial and fungal isolates parallel to wastewater in triplicates. The soil analyses were conducted in three intervals each after 20, 30, and 40 days. The combination of FeBC 3%+F2 reduced the soil DTPA-extractable chromium by 96.8% after 40 days of incubation (DAI) relative to wastewater. Similarly, 92.81% reduction in chromium concentration was achieved through ZnBC 3%+B1 after 40 DAI compared to wastewater. Under the respective treatments, soil Cr(VI) retention trend increased with time such as 40 > 30 > 20 DAI. Langmuir adsorption isotherm verified the highest chromium adsorption capacity (41.6 mg g-1) with FeBC 3% at 40 DAI. Likewise, principal component analysis (PCA) and heat map disclosed electrical conductivity-chromium positive, while cation exchange capacity-chromium and pH-organic matter negative correlations. PCA suggested the ZnBC-bacterial while FeBC-fungal combinations as effective Cr(VI) immobilizers with >70% data variance at 40 DAI. Overall, the study showed that microbes + ZnBC/FeBC resulted in low pH, high OM, and CEC, which ultimately played a role in maximum Cr(VI) adsorption from wastewater applied to the soil. The study also revealed the interrelation and alternations in soil dynamics with pollution control treatments. Based on primitive soil characteristics such as soil metal concentration, its acidity, and alkalinity, the selection criteria can be set for treatments application to regulate the soil properties. Additionally, FeBC with Trichoderma viride should be tested on the field scale to remediate the Cr(VI) toxicity.

Genetic Association of Beta-Myosin Heavy-Chain Gene (MYH7) with Cardiac Dysfunction.

Cardiac dysfunction accelerates the risk of heart failure, and its pathogenesis involves a complex interaction between genetic and environmental factors. Variations in myosin affect contractile abilities of cardiomyocytes and cause structural and functional abnormalities in myocardium. The study aims to find the association of MYH7 rs121913642 (c.1594 T>C) and rs121913645 (c.667G>A) variants with cardiac dysfunction in the Punjabi Pakistani population. Patients with heart failure (n = 232) and healthy controls (n = 205) were enrolled in this study. MYH7 variant genotyping was performed using tetra ARMS-PCR. MYH7 rs121913642 TC genotype was significantly more prevalent in the patient group (p < 0.001). However, MYH7 rs121913645 genotype frequencies were not significantly different between the patient and control groups (p < 0.666). Regression analysis also revealed that the rs121913642 C allele increases the risk of cardiac failure by ~2 [OR:1.98, CI: 1.31−2.98, p < 0.001] in comparison to the T allele. High levels of the cardiac enzymes cardiac troponin I (cTnI) and CK-MB were observed in patients. There was also an increase in total cholesterol, LDL cholesterol, and uric acid in patients compared to the healthy control group (p < 0.001). In conclusion, the MYH7 gene variant rs121913642 is genetically associated with cardiac dysfunction and involved in the pathogenesis of HF.