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1.
Klin Khir ; (4): 9-11, 2016 Apr.
Artigo em Ucraniano | MEDLINE | ID: mdl-27434945

RESUMO

Pathophysiological mechanisms of the vitamin K impact, including those in the gut with ulcerative affection, are studied still insufficiently. Investigations of pharmacogenomics of the vitamin K gives a new approach to therapy in patients, suffering gastro-intestinal hemorrhage. Possibilities of titration of the vitamin K3 (menadione) doses, depending on level of estrogenemia and genetic constitution, concerning genes-candidates ESR1 (rs2234693) and VKORC1 (rs9923231), were studied. There were examined 36 patients, who were treated for the ulcer hemorrhage. The blood serum concentration of estradiol was investigated in accordance to method of solid phase enzyme immunoassay, the genotyping procedure was performed in accordance to indices of polymerase chain reaction with analysis of the restrictional fragments length. The initial daily dose of menadione have constituted 20 mg. After a genotype determination made (first-second day after admittance to hospital) in patients with normoestrogenemia in genotypes CC/GG, CC/GA, CT/GG, CT/GA a vitaminotherapy was prolonged in daily dose of 20 mg, and in a conditionally-pathological variant of genotype the dose of vitamin K was enhanced up to 30 mg. Determination of hormones and the patients' genetic constitution makes possible to apply a personified approach for the vitamin K3 application in the ulcerative hemorrhage.


Assuntos
Anticoagulantes/uso terapêutico , Úlcera Duodenal/tratamento farmacológico , Receptor alfa de Estrogênio/genética , Hemorragia Gastrointestinal/tratamento farmacológico , Úlcera Gástrica/tratamento farmacológico , Vitamina K Epóxido Redutases/genética , Vitamina K/uso terapêutico , Adulto , Cálculos da Dosagem de Medicamento , Úlcera Duodenal/sangue , Úlcera Duodenal/genética , Úlcera Duodenal/patologia , Estradiol/sangue , Receptor alfa de Estrogênio/sangue , Feminino , Hemorragia Gastrointestinal/sangue , Hemorragia Gastrointestinal/genética , Hemorragia Gastrointestinal/patologia , Expressão Gênica , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Polimorfismo de Nucleotídeo Único , Medicina de Precisão , Úlcera Gástrica/sangue , Úlcera Gástrica/genética , Úlcera Gástrica/patologia , Vitamina K Epóxido Redutases/sangue
2.
Klin Khir ; (2): 13-5, 2015 Feb.
Artigo em Ucraniano | MEDLINE | ID: mdl-25985687

RESUMO

Expression of alpha-receptors of estrogen (RE) in accordance to immunohistochemical (IHC) labeling in gastroduodenal mucosa cells was studied up in patients, suffering the ulcer disease and without it. In 4 patients (group I) a gastroduodenal mucosa affection was revealed, they were operated on for hemorrhage from gastroduodenal ulcers; in 3 patients (group II) gastroduodenal mucosa affection was not observed; in 4 patients (group III, control), a mammary gland cancer was diagnosed, a positive reaction on alpha-RE was noted. In groups I and II the biopsies were studied, obtained from pylorus and gastric fundus, as well as from duodenal ampula, and in a group III--obtained from the tumor. In a control group a positive labeling of nuclei was revealed in biopsies. In patients of groups I and II the alpha-RE expression by cellular nuclei was not revealed, but, the lots of positive IHC labeling of cytoplasm in glandular and stromal mucosal cells of the investigated gut were noted. Positive IHC labeling of cytoplasm for alpha-RE witnesses about sensitivity to them in norma and pathological processes. But, a trustworthy difference of alpha-RE expression by cellular nuclei was not noted. For confirmation or denial of this hypothesis further clinical and IHC investigations are needed.


Assuntos
Neoplasias da Mama/metabolismo , Úlcera Duodenal/metabolismo , Receptor alfa de Estrogênio/metabolismo , Estrogênios/metabolismo , Úlcera Péptica Hemorrágica/metabolismo , Úlcera Gástrica/metabolismo , Adulto , Idoso , Neoplasias da Mama/complicações , Neoplasias da Mama/patologia , Núcleo Celular/efeitos dos fármacos , Núcleo Celular/metabolismo , Núcleo Celular/patologia , Úlcera Duodenal/complicações , Úlcera Duodenal/patologia , Duodeno/efeitos dos fármacos , Duodeno/metabolismo , Duodeno/patologia , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Receptor alfa de Estrogênio/genética , Estrogênios/farmacologia , Feminino , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/metabolismo , Mucosa Gástrica/patologia , Expressão Gênica , Humanos , Imuno-Histoquímica , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Masculino , Pessoa de Meia-Idade , Úlcera Péptica Hemorrágica/complicações , Úlcera Péptica Hemorrágica/patologia , Estômago/efeitos dos fármacos , Estômago/patologia , Úlcera Gástrica/complicações , Úlcera Gástrica/patologia
3.
Klin Khir ; (11): 24-7, 2015 Nov.
Artigo em Ucraniano | MEDLINE | ID: mdl-26939421

RESUMO

Genetic-hormonal regulation plays a key pathophysiologic role in a blood loss on background of complicated gastroduodenal ulcer disease, but a clinical significance of some genes of compensatory steroidogenesis remains unrevealed. Examination of 63 patients, using a chain reaction with polymerase (CRP); analysis of length of restriction fragments (CRP-RFLP) and immunohistochemical investigation of gastroduodenal mucosa were performed on the base of a Sumsky Rural Clinical Hospital. Trustworthy difference of distribution of polymorphic genes ESR1 and VKORC1 in patients of both gender in presence of the ulcer hemorrhage was not revealed, excluding genotype A/A VKORC1, what trustworthy more frequently was revealed in women (p < 0.05). There was established, that intact zone of gastric fundus owes immunoreactivity towards alpha-receptors of estrogen in nuclei of epitheliocytes and stromocytes. Diagnosis of polymorphic gene VKORC1 and expression of the estrogen receptors may serve the base for pathogenetic therapy in patients with hemorrhage occurrence.


Assuntos
Receptor alfa de Estrogênio/genética , Úlcera Péptica Hemorrágica/genética , Úlcera Péptica/genética , Polimorfismo de Nucleotídeo Único , Vitamina K Epóxido Redutases/genética , Alelos , Núcleo Celular/metabolismo , Núcleo Celular/patologia , Células Epiteliais/metabolismo , Células Epiteliais/patologia , Receptor alfa de Estrogênio/metabolismo , Feminino , Mucosa Gástrica/metabolismo , Mucosa Gástrica/patologia , Expressão Gênica , Frequência do Gene , Testes Genéticos , Genótipo , Humanos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Masculino , Úlcera Péptica/metabolismo , Úlcera Péptica/patologia , Úlcera Péptica Hemorrágica/metabolismo , Úlcera Péptica Hemorrágica/patologia , Fatores Sexuais , Vitamina K Epóxido Redutases/metabolismo
4.
Klin Khir ; (4): 38-40, 2011 Apr.
Artigo em Ucraniano | MEDLINE | ID: mdl-21698933

RESUMO

Possibilities of modern antibacterial therapy of pulmonary tuberculosis do not satisfy specialists. To apply a standard resection methods is not always possible because of the extended process present. The authors had summarized the experience of performance of simultaneous bilateral transsternal pulmonary resection for different forms of tuberculosis in 23 patients, proposing it as an alternative to present resectional methods.


Assuntos
Procedimentos Cirúrgicos Pulmonares/métodos , Esternotomia/métodos , Tuberculose Pulmonar/cirurgia , Adulto , Humanos , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento , Adulto Jovem
5.
Izv Akad Nauk SSSR Biol ; (4): 575-81, 1992.
Artigo em Russo | MEDLINE | ID: mdl-1280659

RESUMO

A bioenergetic conception of insulin-independent diabetes mellitus (IIDM) is put forward. Molecular mechanisms of carbohydrate and fat metabolism are discussed which result in a vicious regulatory cycle to provoke a transition to stable pathology. Previously unknown role of human saliva in oral cavity in this regulatory cycle is revealed. New diagnostic tests are developed on the basis of the conception on IIDM pathogenesis. The results of clinical trials are presented which have been obtained during population screening.


Assuntos
Metabolismo dos Carboidratos , Diabetes Mellitus Tipo 2/diagnóstico , Diabetes Mellitus Tipo 2/etiologia , Diabetes Mellitus Tipo 2/metabolismo , Diagnóstico Diferencial , Metabolismo Energético , Ácidos Graxos/metabolismo , Glucose/análise , Humanos , Saliva/química , Saliva/metabolismo , Coloração e Rotulagem
6.
Izv Akad Nauk SSSR Biol ; (1): 148-51, 1991.
Artigo em Russo | MEDLINE | ID: mdl-1856359

RESUMO

Mucine-like glycoproteins have been studied by structural indication. Textures of these compounds have been described. Possibility for using mucines as matrix structures in microcrystallographic method of analysis of complex multicomponent systems has been discussed.


Assuntos
Mucinas/ultraestrutura , Proteínas e Peptídeos Salivares/ultraestrutura , Cromatografia em Agarose , Cromatografia em Gel , Cristalografia , Glicosilação , Humanos , Mucinas/química , Saliva/química , Proteínas e Peptídeos Salivares/química
7.
Izv Akad Nauk SSSR Biol ; (1): 58-63, 1989.
Artigo em Russo | MEDLINE | ID: mdl-2715495

RESUMO

Acidic and alkaline RNase activity from healthy humans and gastric cancer patients has been studied. A decrease in daily saliva production and an increase in RNase activity was detected saliva of cancer patients. This suggests the existence of RNase inhibitors in healthy humans. This supposition is further confirmed by comparative analysis of total, joint fractional and reconstituted RNase activity. A considerable increase in acidic RNase inhibitors and disappearance of alkaline RNase inhibitor was observed in cancer patients. The specificity, mechanisms and clinical significance of this phenomena has been discussed.


Assuntos
Ribonucleases/análise , Saliva/enzimologia , Neoplasias Gástricas/enzimologia , Ritmo Circadiano , Humanos , Concentração de Íons de Hidrogênio , Masculino , Pessoa de Meia-Idade , Valores de Referência , Ribonucleases/antagonistas & inibidores , Saliva/análise , Proteínas e Peptídeos Salivares/análise
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