RESUMO
The paper provides a review of the data available in the literature on biosensors based on plant and animal tissues. It presents the enzymes contained in the tissues, by describing the respective biochemical reactions underlying these sensors operation. It also gives basic characteristics of the biosensors in question.
Assuntos
Técnicas Biossensoriais , Animais , Humanos , Modelos BiológicosRESUMO
A panel of hybridoma clones producing monoclonal antibodies (Mabs) to the antigens of synaptosomes from rat brain cortex was generated. In immunocytochemical experiments 5F5-B6 Mabs stained stratum moleculare in hippocampus and cerebellum of adult rats intraventricular introduction of 5F5-B6 Mabs was shown to block LTP formation in fascia dentata and to change the evoked potential and population spike amplitude.
Assuntos
Anticorpos Monoclonais/farmacologia , Hipocampo/efeitos dos fármacos , Potenciação de Longa Duração/efeitos dos fármacos , Animais , Anticorpos Monoclonais/isolamento & purificação , Biomarcadores , Córtex Cerebral/imunologia , Estimulação Elétrica , Potenciais Evocados/efeitos dos fármacos , Potenciais Evocados/imunologia , Hipocampo/imunologia , Hipocampo/fisiologia , Hibridomas/imunologia , Imunização , Imuno-Histoquímica , Potenciação de Longa Duração/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Ratos , Ratos Wistar , Sinaptossomos/imunologiaRESUMO
Male rats of the strains with low (LE) high excitability (HE) of the nervous system have been used in this study. Half of the animals of each strain were neurotized in accordance with the Hecht's scheme. In the hippocampal slices of the non-neurotized LE rats there was a significant increase of the populational spike amplitude during development of LTP as compared with the opposite group of the animals. The LTP formation in the LE strain of rats caused a decrease in the S-100 protein content in the water-soluble, and an increase in the membrane-bound fraction of the protein. Similar results we have observed with the non-inbred Wistar rats but not with the HE strain of the animals. The levels of the water-soluble S-100 protein fraction were also higher in the hippocampuses and entorenal cortices, but not in the cerebellae of the LE strain, as compared with the HE strain of the rats. No differences have been found in the membrane-bound fraction of S-100 protein.
Assuntos
Hipocampo/química , Fenômenos Fisiológicos do Sistema Nervoso , Proteínas S100/análise , Animais , Comportamento Animal , Ácido Edético/farmacologia , Estimulação Elétrica , Hipocampo/efeitos dos fármacos , Hipocampo/fisiologia , Técnicas In Vitro , Masculino , Transtornos Neuróticos/fisiopatologia , Ratos , Ratos Wistar , Tetania , Fatores de TempoRESUMO
It has been revealed that intraperitoneal injection of T-activin (humoral factor of the thymus) to August rats leads to more rapid and stable conditioned reflex formation to a sound and to a decrease of avoidance time when electric current is given to a shuttle chamber. Furthermore, less amount of uneffective series in testing unconditioned avoidance is registered in the test animals. A positive T-activin effect on conditioned reflex formation and unconditioned reflex manifestation is probably connected with its ability to alter hippocampus functional parameters and (or) with anti-stressor properties of the preparation.
Assuntos
Adjuvantes Imunológicos/farmacologia , Aprendizagem da Esquiva/efeitos dos fármacos , Condicionamento Clássico/efeitos dos fármacos , Peptídeos/farmacologia , Extratos do Timo/farmacologia , Animais , Hipocampo/efeitos dos fármacos , Masculino , RatosAssuntos
Anticorpos/imunologia , Encéfalo/imunologia , Aprendizagem , Neurônios/imunologia , Potenciais de Ação , Animais , Antígenos de Superfície/imunologia , Encéfalo/fisiologia , Eletroencefalografia , Canais Iônicos/fisiologia , Potenciais da Membrana , Neurônios/fisiologia , Ratos , Ratos EndogâmicosRESUMO
The influence of adrenochrome on the electrical characteristics of Helix pomatia neurons was examined. Application of adrenochrome transformed neuronal regular rhythmic activity into bursting one. Anomalous rectification was seen. The voltage clamp technique showed that the action of adrenochrome lowered both inward and outward currents.
Assuntos
Adrenocromo/farmacologia , Caracois Helix/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Potenciais de Ação/efeitos dos fármacos , Animais , Membrana Celular/efeitos dos fármacos , Potenciais da Membrana/efeitos dos fármacos , Neurônios/fisiologiaRESUMO
Helix pomatia neurons were used as test-objects in the study of adrenochrome effect on nerve tissue by the microelectrode technique and voltage clamp. The action of adrenochrome consists in slow de- and hyperpolarization of the neuronal membrane and in the appearance of periodic short-term shifts in the membrane potential. The lowering of the action potential amplitude is as consequence of the decreased maximal conduction of inward current ionic channels.
Assuntos
Adrenocromo/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Potenciais de Ação/efeitos dos fármacos , Animais , Gânglios/citologia , Caracois HelixRESUMO
Immunofluorescence techniques were used to show that S100 is present on the surface of neuronal and glial membranes of Helix pomatia in vitro. By the method of rocket immunoelectrophoresis of aqueous , Trition, and n-pentanol extracts of snail nervous tissue, S100 was demonstrated to be mainly in the membrane fraction. Anti-S100 antiserum inhibited the electrical activity of identified neurons, pointing to a relationship of this process with ionic channels of the excitable membrane. The effect of anti-S100 antiserum on the membrane was potential dependent and controlled by the Ca2+ concentration.
Assuntos
Gânglios/fisiologia , Proteínas do Tecido Nervoso/fisiologia , Neuroglia/fisiologia , Neurônios/fisiologia , Proteínas S100/fisiologia , Potenciais de Ação , Animais , Imunofluorescência , Caracois Helix , Soros Imunes , Imunoeletroforese , Potenciais da Membrana , Neuroglia/citologia , Neurônios/citologia , Proteínas S100/análiseRESUMO
The existence of cross (common) proteins-antigens on the membrane of nervous and glial cells of Helix pomatia in vitro was proved by Coons' method of immunofluorescence. The presence of the neurospecific protein S-100 on the membrane of these cells and antigenous heterogeneity of the membranes of the neuronal cell population were established. The antigen concentration was shown to vary on the somatic and axonal membranes. The distribution pattern of specific luminescence indicates potential qualitative and/or quantitative differences in the content of neurospecific proteins in various portions of the neuronal membrane.