Predicting antioxidant activity of wood vinegar using color and spectrophotometric parameters.

Wood vinegar can be produced from many types of raw materials using different pyrolysis methods resulting in potentially different antioxidant activity. Therefore, this study developed a rapid method to predict the antioxidant activity of wood vinegar based on color using the CIELAB system and spectrophotometric parameters. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power (FRAP) exhibited significant correlations with the L* and b* values of the color parameters and the UV absorbance polyphenol index (I280), European Brewing Convention (EBC) and Institute of Brewing (IOB) color units, color intensity and Linner Hue index of the spectrophotometric parameters. As a result, DPPH radical scavenging activity and FRAP could be predicted by measuring color and spectrophotometric parameters. Overall, this study provide a simple, rapid and no chemical reaction involved method to predict antioxidant activity. Furthermore, models with a set of spectrophotometric parameters could be used to predict antioxidant activities. •Whole new method for predicting wood vinegar antioxidant activity was developed.•The method was models developed by using color and spectrophotometric parameters used in beverage industry.•The models were simple, rapid and involved no chemical reaction.

Diversity of coronavirus in bats from Eastern Thailand.

BACKGROUND: Bats are reservoirs for a diverse range of coronaviruses (CoVs), including those closely related to human pathogens such as Severe Acute Respiratory Syndrome (SARS) CoV and Middle East Respiratory Syndrome CoV. There are approximately 139 bat species reported to date in Thailand, of which two are endemic species. Due to the zoonotic potential of CoVs, standardized surveillance efforts to characterize viral diversity in wildlife are imperative. FINDINGS: A total of 626 bats from 19 different bat species were individually sampled from 5 provinces in Eastern Thailand between 2008 and 2013 (84 fecal and 542 rectal swabs). Samples collected (either fresh feces or rectal swabs) were placed directly into RNA stabilization reagent, transported on ice within 24 hours and preserved at -80°C until further analysis. CoV RNA was detected in 47 specimens (7.6%), from 13 different bat species, using broadly reactive consensus PCR primers targeting the RNA-Dependent RNA Polymerase gene designed to detect all CoVs. Thirty seven alphacoronaviruses, nine lineage D betacoronaviruses, and one lineage B betacoronavirus (SARS-CoV related) were identified. Six new bat CoV reservoirs were identified in our study, namely Cynopterus sphinx, Taphozous melanopogon, Hipposideros lekaguli, Rhinolophus shameli, Scotophilus heathii and Megaderma lyra. CONCLUSIONS: CoVs from the same genetic lineage were found in different bat species roosting in similar or different locations. These data suggest that bat CoV lineages are not strictly concordant with their hosts. Our phylogenetic data indicates high diversity and a complex ecology of CoVs in bats sampled from specific areas in eastern regions of Thailand. Further characterization of additional CoV genes may be useful to better describe the CoV divergence.