Depth-Resolved Attenuation Mapping of the Vaginal Wall under Prolapse and after Laser Treatment Using Cross-Polarization Optical Coherence Tomography: A Pilot Study.

Vaginal wall prolapse is the most common type of pelvic organ prolapse and is mainly associated with collagen bundle changes in the lamina propria. Neodymium (Nd:YAG) laser treatment was used as an innovative, minimally invasive and non-ablative procedure for the treatment of early-stage vaginal wall prolapse. The purpose of this pilot study was to assess connective tissue changes in the vaginal wall under prolapse without treatment and after Nd:YAG laser treatment using cross-polarization optical coherence tomography (CP OCT) with depth-resolved attenuation mapping. A total of 26 freshly excised samples of vaginal wall from 26 patients with age norm (n = 8), stage I-II prolapses without treatment (n = 8) and stage I-II prolapse 1-2 months after Nd:YAG laser treatment (n = 10) were assessed. As a result, for the first time, depth-resolved attenuation maps of the vaginal wall in the B-scan projection in the co- and cross-polarization channels were constructed. Two parameters within the lamina propria were target calculated: the median value and the percentages of high (≥4 mm-1) and low (<4 mm-1) attenuation coefficient values. A significant (p < 0.0001) decrease in the parameters in the case of vaginal wall prolapse compared to the age norm was identified. After laser treatment, a significant (p < 0.0001) increase in the parameters compared to the normal level was also observed. Notably, in the cross-channel, both parameters showed a greater difference between the groups than in the co-channel. Therefore, using the cross-channel achieved more reliable differentiation between the groups. To conclude, attenuation coefficient maps allow visualization and quantification of changes in the condition of the connective tissue of the vaginal wall. In the future, CP OCT could be used for in vivo detection of early-stage vaginal wall prolapse and for monitoring the effectiveness of treatment.

Comparative study of soft tissue surgery by visible and infrared laser radiation.

To compare the cutting properties of lasers with different wavelengths (445 nm, 532 nm and 808 nm) used in cutting blood-rich tissues. Porcine myocardial tissue was cut using 2.3-15 W laser radiation in contact and non-contact modes with an optical fiber or focusing handpiece. The cut depth and coagulation zone width were determined histologically. The 445-nm laser achieved the greatest cut depth for all cutting parameters (p < 0.01). The blue laser gave the smallest coagulation width to cut depth ratio. Results of the study are consistent with the assumption that a 445 nm blue laser may have better cutting properties than green and infrared lasers due to the high absorption of radiation at this wavelength in hemoglobin and, consequently, in the biological blood-rich tissue.

Analysis of chlorophyll fluorescence parameters as predictors of biomass accumulation and tolerance to heat and drought stress of wheat (Triticum aestivum) plants.

Agricultural technologies aimed at increasing yields require the development of highly productive and stress-tolerant cultivars. Phenotyping can significantly accelerate breeding; however, no reliable markers have been identified to select the most promising cultivars at an early stage. In this work, we determined the light-induced dynamic of chlorophyll fluorescence (ChlF) parameters in young seedlings of 10 wheat (Triticum aestivum L.) cultivars and evaluated potency of these parameters as predictors of biomass accumulation and stress tolerance. Dry matter accumulation positively correlated with the effective quantum efficiency of photosystem II (Φ PSIIef ) and negatively correlated with the half-time of Φ PSIIef reaching (t 1/2 (Φ PSIIef )). There was a highly significant correlation between t 1/2 (Φ PSIIef ) and dry matter accumulation with increasing prediction period. Short-term heating and drought caused an inhibition of biomass accumulation and photosynthetic activity depending on the stressor intensity. The positive correlation between the Φ PSII dark level (Φ PSIId ) in young seedlings and tolerance to a rapidly increasing short-term stressor (heating) was shown. In the case of a long-term stressor (drought), we revealed a strong negative relationship between tolerance and the level of non-photochemical fluorescence quenching (NPQ). In general, the results show the potency of the ChlF parameters of young seedlings as predictors of biomass accumulation and stress tolerance.

Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry.

Chlorophyll fluorescence imaging was used to study potato virus X (PVX) infection of Nicotiana benthamiana. Infection-induced changes in chlorophyll fluorescence parameters (quantum yield of photosystem II photochemistry (ΦPSII) and non-photochemical fluorescence quenching (NPQ)) in the non-inoculated leaf were recorded and compared with the spatial distribution of the virus detected by the fluorescence of GFP associated with the virus. We determined infection-related changes at different points of the light-induced chlorophyll fluorescence kinetics and at different days after inoculation. A slight change in the light-adapted steady-state values of ΦPSII and NPQ was observed in the infected area of the non-inoculated leaf. In contrast to the steady-state parameters, the dynamics of ΦPSII and NPQ caused by the dark-light transition in healthy and infected areas differed significantly starting from the second day after the detection of the virus in a non-inoculated leaf. The coefficients of correlation between chlorophyll fluorescence parameters and virus localization were 0.67 for ΦPSII and 0.76 for NPQ. In general, the results demonstrate the possibility of reliable pre-symptomatic detection of the spread of a viral infection using chlorophyll fluorescence imaging.

Participation of calcium ions in induction of respiratory response caused by variation potential in pea seedlings.

Electrical signals in plants caused by external stimuli are capable of inducing various physiological responses. The mechanisms of transformation of a long-distance electrical signal (ES) into a functional response remain largely unexplored and require additional research. In this work, we investigated the role of calcium ions in the development of ES-induced respiratory response. Gradual heating of the leaf causes the propagation of variation potential (VP) in the pea seedling. The propagation of VP leads to a transient activation of respiration in an unaffected leaf. During the VP generation, a transient increase in the intracellular calcium concentration takes place. A calcium channel blocker inhibits the respiratory response, and a calcium ionophore induces the activation of respiration. Inhibitory analysis has showed that the VP-induced increase in respiration activity is probably associated with calcium-mediated activation of rotenone-insensitive alternative NADPH dehydrogenases in mitochondria.

Simulation of a nonphotochemical quenching in plant leaf under different light intensities.

An analysis of photosynthetic response on action of stressors is an important problem, which can be solved by experimental and theoretical methods, including mathematical modeling of photosynthetic processes. The aim of our work was elaboration of a mathematical model, which simulated development of a nonphotochemical quenching under different light conditions. We analyzed two variants of the model: the first variant included a light-induced activation of the electron transport chain; in contrast, the second variant did not describe this activation. Both variants of the model described interactions between transitions from open reaction centers to closed ones (and vice versa) and development of the nonphotochemical quenching. Investigation of both variants of the model showed well qualitative and quantitative accordance between simulated and experimental changes in coefficient of the nophotochemical quenching which were analyzed under different light regimes: (i) the stepped increase of the light intensity without dark intervals between steps, (ii) periodical illuminations by different light intensities with constant durations which were separated by constant dark intervals, and (iii) periodical illuminations by the constant light intensity with different durations which were separated by different dark intervals. Thus, the model can be used for theoretical prediction of stress changes in photosynthesis under fluctuations in light intensity and search of optimal regimes of plant illumination.