Pollution impact on microbial communities composition in natural and anthropogenically modified soils of Southern Russia.

Metagenomic studies of soil microbocenoses are extremely relevant nowadays. The study of pollution impact on soil microbiomes is of particular interest. The structure of microbial communities in soils with different levels of pollution by polycyclic aromatic hydrocarbons (PAHs) and potentially toxic elements (PTEs) was studied. High bacterial biodiversity was found in all the studied soil samples, but its lowest values are found in soil samples taken on the territory of technogenically polluted Lake Atamanskoye. Assessment of soil pollution showed the highest content of polycyclic aromatic hydrocarbons (PAHs) and potentially toxic elements (PTEs) for the soils Lake Atamanskoye. The high content of pollutants negatively affects the abundance of representatives of the phyla Actinobacteria, Planctomycetes, Verrucomicrobia, and Nitrospirae. Such phyla as Proteobacteria, Candidate Divisions TM7, OD1, WPS-2, Chlamydiae, Cyanobacteria are characterized by positive direct correlation with the content of pollutants, especially with PAHs. A cooperative effect of decrease in the number of Actinobacteria and Proteobacteria with an increase in Armatimonadetes probably corresponds to PTEs contamination. The proportion of Candidate Division OD1, Chlamydiae, Cyanobacteria, and Candidate Division WPS-2 was increased in the soil microbiome under the influence of severe combined pollution. Pollutants negatively affect the abundance of dominant unclassified_o__Gaiellales and unclassified_o__WD2101 genera. Iamia, Salinibacterium, Arthrobacter, Kaistobacter, Thiobacillus genera are characterized by a low abundance, but they are presumably the most resistant to soil pollution. It was revealed that the level of soil pollution largely determines the composition and diversity of bacterial communities in the soils of the studied territories. Operating taxonomic units have been established that have prognostic value for assessing the state, level of soil pollution, and their biological safety.

Polycyclic aromatic hydrocarbons, antibiotic resistance genes, toxicity in the exposed to anthropogenic pressure soils of the Southern Russia.

The influence of anthropogenic pollution, particularly with polycyclic aromatic hydrocarbons (PAHs) on soil toxicity and spread of antibiotic resistance genes (ARGs) is extremely important nowadays. We studied 20 soil samples from a technogenically polluted site, municipal solid wastes (MSW) landfills, and rural settlements in the southwestern part of the Rostov Region of Russia. A close correlation was established between the results of biosensor testing for integral toxicity, the content of genes for the biodegradation of hydrocarbons, and the concentration of PAHs in soils. The relation between the quantitative content of ARGs and the qualitative and quantitative composition of PAHs has not been registered. Soils subjected to different types of the anthropogenic pressure differed in PAHs composition. The technogenic soils are the most polluted ones. These soils are enriched with 5 ring PAHs and carry the maximum variety of assayed ARGs, despite the fact that they do not receive household or medical waste.

Antioxidant enzymes and reactive oxygen species level of the Achromobacter xylosoxidans bacteria during hydrocarbons biotransformation.

The level of catalase and superoxide dismutase induction, as well as generation of superoxide anion radical in cells and accumulation of hydrogen peroxide in the culture medium were researched in three strains of oil-degrading bacteria Achromobacter xylosoxidans at cultivation in rich nutrient medium and in the media with hydrocarbons as the only source of carbon. The effects of pentane, decane, hexadecane, cyclohexane, benzene, naphthalene and diesel fuel were evaluated. It was determined that in the microbial cell on media with hydrocarbons, the generation of superoxide anion radical increases, accumulation of hydrogen peroxide and induction of superoxide dismutase synthesis occur, and catalase activity is reduced. Oxidative stress in the cells of A. xylosoxidans was caused by biotransformation of all the studied hydrocarbons. The most pronounced effect was observed at incubation of bacteria with cyclohexane, pentane, diesel fuel, benzene and naphthalene.

Distribution of polycyclic aromatic hydrocarbons in surface sediments of lower reaches of the Don River (Russia) and their ecotoxicologic assessment by bacterial lux-biosensors.

The lower Don River in the south of the European part of Russia was studied to determine the concentration, spatial distribution, and sources of 19 polycyclic aromatic hydrocarbons (PAHs) in surface sediments. Total PAH concentrations ranged from 14.2 to 529 ng/g-dw. Sedimentary PAH concentrations were higher in the delta of the Don River and in the estuaries of rivers Sal and Aksai compared to the main channel of the Don. Analysis of the PAHs sources showed that PAHs came mostly from pyrogenic sources as a result of incomplete combustion of coal. Bioluminescent bacterial sensors were used for ecotoxicological assessment of surface sediments. The surface sediments of all the investigated stations of lower reaches of the Don River were toxic and genotoxic. The maximum concentration of PAHs and the high genotoxicity effect caused by the presence of genotoxicants were found in the surface sediments of the same stations. Significant correlations between the concentrations of individual PAHs in sediments and the genotoxic effect were found. Correlation between genotoxicity of surface sediments and concentration of phenanthrene and benz(k)fluoranthene was the most significant both with and without application of metabolic activation.