RESUMO
The effect of extremely weak human electromagnetic field on the suspensions of Escherichia coli and Staphylococcus aureus in a physiological solution at 25 degrees C was studied. The results obtained indicate that the human field induces changes in the physical properties of liquid water in physiological solution and the induced structural and dynamic rearrangements of extracellular water are tranferred to bacterial cells and change their functional activity.
Assuntos
Campos Eletromagnéticos , Escherichia coli/efeitos da radiação , Staphylococcus aureus/efeitos da radiação , HumanosAssuntos
Transfusão de Sangue Autóloga/métodos , Sangue/efeitos da radiação , Procedimentos Cirúrgicos Operatórios , Terapia Ultravioleta/métodos , Adulto , Idoso , Transfusão de Sangue Autóloga/normas , Terapia Combinada , Relação Dose-Resposta à Radiação , Feminino , Homeostase/efeitos da radiação , Humanos , Medições Luminescentes , Masculino , Pessoa de Meia-Idade , Terapia Ultravioleta/normasRESUMO
The use of the method of autologous blood photomodification in surgery is discussed on basis of generalized experience of the Leningrad Research-Practical Center of Blood Photomodification (3,000 procedures conducted in over 2,000 patients). The current state of the problem, the main mechanisms of the therapeutic action of the procedure, and the clinical efficacy of the method in various surgical diseases are dealt with. The possible complications and the means of their prevention are deal with. The methodological aspects of research are discussed. The authors define the main problems which must be solved in order to raise the efficacy of using photomodification of autologous blood in surgery.
Assuntos
Transfusão de Sangue Autóloga , Sangue/efeitos da radiação , Procedimentos Cirúrgicos Operatórios , Infecção da Ferida Cirúrgica/radioterapia , Toxemia/radioterapia , Terapia Ultravioleta/métodos , Humanos , Procedimentos Cirúrgicos Operatórios/efeitos adversos , Infecção da Ferida Cirúrgica/prevenção & controle , Toxemia/prevenção & controleRESUMO
The paper is concerned with the results of a study of the effect of single reinfusion of photomodified autoblood on the level of plasma hormones, lipid peroxidation and antioxidant system of erythrocytes, the activity of the main enzymes of glucose metabolism in erythrocytes, the state of cellular immunity in 45 patients with non-insulin-dependent diabetes mellitus (NIDDM) in 1.7 and 14 days after autoblood photomodification (BPM). BPM was shown to cause a prolonged effect of the activation of intracellular glucose metabolism, a decrease in the endogenous insulin consumption by tissues, and a decrease in the blood concentration of hormones of contrainsular action. The use of BPM enhanced lipid peroxidation and the activity of the antioxidant system of erythrocytes. Higher activity of immunocompetent lymphocytes was in line with the time course of the hormone level. The authors proposed a hypothesis accounting for a many-sided effect of BPM on glucose metabolism and hormonal changes in NIDDM patients.
Assuntos
Transfusão de Sangue Autóloga , Sangue/efeitos da radiação , Diabetes Mellitus Tipo 2/terapia , Luz , Adulto , Idoso , Glicemia/metabolismo , Terapia Combinada , Diabetes Mellitus Tipo 2/sangue , Eritrócitos/enzimologia , Eritrócitos/metabolismo , Feminino , Hormônios/sangue , Humanos , Peroxidação de Lipídeos , Linfócitos/metabolismo , Masculino , Pessoa de Meia-Idade , OxirreduçãoRESUMO
The absorbance and EPR spectra of type 1 and 2 copper-binding centres which are present in ceruloplasmin (Cp) molecule were shown to disappear upon the reduction of the enzyme by ascorbate under anaerobic conditions. The fluorescence band attributed to type 3 Cu was altered concomitantly. The electron-accepting nitroxyl radical added to reduced Cp restored the absorbance, EPR and fluorescence spectra of the oxidase. Only type 1 and 3 copper ions, as judged by spectral changes, can be reduced by ascorbate and then reoxidized by the nitroxyl radical in the azide-treated Cp. The spectral properties of Cp provided by copper ions of different types change simultaneously and concordantly upon oxidation/reduction. This seems to be caused by cooperative interaction of these ions involved in the electron transfer from the donating substrate to the accepting molecule of the nitroxyl radical (in model studies of oxidase reaction) or oxygen (under natural conditions). The copper ions in the active centre of Cp constitute an intramolecular electron transport chain, which may, at least in vitro, function without one of its links.
Assuntos
Ceruloplasmina/metabolismo , Oxirredutases , Ácido Ascórbico/metabolismo , Sítios de Ligação , Cobre/metabolismo , Espectroscopia de Ressonância de Spin Eletrônica , Transporte de Elétrons , Radicais Livres , Medições Luminescentes , Oxirredução , Análise EspectralRESUMO
The method of UV irradiation of autoblood followed by infusion in the early postoperative period was used in 109 patients subjected to various operative interventions on the small pelvis organs. It was established that the infusion of autologous photomodified blood allowed to avoid postoperative thromboembolic complications.
Assuntos
Transfusão de Sangue Autóloga/métodos , Sangue/efeitos da radiação , Pelve/cirurgia , Complicações Pós-Operatórias/prevenção & controle , Tromboembolia/prevenção & controle , Raios Ultravioleta , HumanosRESUMO
Near-UV irradiation (280-365 nm) at non-lethal doses increased lymphocyte E and EAC rosette-forming capacity, reduced cell proliferation in response to mitogen (PHA), induced an increase in the content of lipid peroxidation products in the cell culture medium. An antioxidant (alpha-tocopherol, 10(-7) M) administered before or immediately after near UV irradiation of lymphocytes reduced the above effects. The addition of an antioxidant to the culture medium 90 min after cell irradiation failed to reduce lymphocyte rosette-forming capacity. Near-UV irradiation of the blood reduced cell proliferative response to PHA. alpha-tocopherol (10(-7) M) administered before and immediately after the blood photomodification blocked the suppression of cell proliferation in response to mitogen.
Assuntos
Linfócitos B/efeitos da radiação , Linfócitos T/efeitos da radiação , Adulto , Linfócitos B/efeitos dos fármacos , Linfócitos B/imunologia , Células Cultivadas , Humanos , Ativação Linfocitária/efeitos dos fármacos , Ativação Linfocitária/efeitos da radiação , Masculino , Fito-Hemaglutininas/farmacologia , Formação de Roseta , Linfócitos T/efeitos dos fármacos , Linfócitos T/imunologia , Fatores de Tempo , Raios Ultravioleta , Vitamina E/farmacologiaAssuntos
Linfócitos/efeitos da radiação , Raios Ultravioleta , Animais , Antígenos de Superfície/efeitos da radiação , Medula Óssea/imunologia , Medula Óssea/efeitos da radiação , Linfócitos/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C3H , Baço/imunologia , Baço/efeitos da radiação , Propriedades de Superfície , Timo/imunologia , Timo/efeitos da radiaçãoRESUMO
It has been found that under UV-irradiation of the blood serum at 77 K free radicals are formed as a result of photodestruction of protein molecules. The protein molecule fragments play a role of antigens which during the transfusion of blood (blood serum) initiate immune reactions bringing about polyimmunization of human organism.