Assuntos
Neoplasias de Tecido Nervoso/classificação , Astrocitoma/classificação , Neoplasias Encefálicas/classificação , Ependimoma/classificação , Ganglioneuroma/classificação , Glioma/classificação , Humanos , Meduloblastoma/classificação , Neoplasias de Tecido Nervoso/patologia , Neurilemoma/classificação , Neuroblastoma/classificação , Oligodendroglioma/classificação , Glândula Pineal , Terminologia como AssuntoRESUMO
A rather distinct correlation in the content and distribution of DNA with the grade of malignancy was estabilished for the following series: normal astrocytes, and oligodendrocytes, typical astrocyfomas and oligodendrogliomas atypical astrocytomas and oligodendrogliomas. For glioblastomas this correlation is impaired, because the polymorphic variant of glioblastoma is characterized by particularly great disturbances in the content and distribution of DNA in the form of polyploidy and aneuploidy which, in this case, reflects not only the grade of malignancy, but also a pronounced polymorphism of tumour cells. The content of DNA in rapidly growing malignant tumours is not only an expressions of real deviation to the side of polyploidy and the mitotic cycle. In neuroectodermal tumours growing in the condition of cell culture, the similar regularity was observed, although the phenomena of aneuploidy were more distinctly expressed in the majority of tumours.
Assuntos
Neoplasias Encefálicas/metabolismo , DNA de Neoplasias/metabolismo , Aneuploidia , Astrocitoma/metabolismo , Neoplasias Encefálicas/genética , Células Cultivadas , Ependimoma/metabolismo , Histocitoquímica , Humanos , Meduloblastoma/metabolismo , Oligodendroglioma/metabolismoRESUMO
The activity of 8 enzymes (dehydrogenase and diaphorase) in cells of 110 neuroectodermal tumours was studied. The overall assessment showed that the activity of these enzymes varied: the highest was noted for NAD-diaphorase and lactadehydrogenase, the lowest--for enzymes of Krebs' cycle. The activity of dehydrogenase and diaphorase was different in neuroectodermal tumours of different origin. Not infrequently, there was observed "enzymatic polymorphism" of the cells of one and the same tumour. A higher activity of these enzymes in tumour cells, as a rule, correlated with a greater amount of cytoplasma and with a shift to the latter of the nucleo-cytoplasmatic ratio. Metabolism of tumour and reactively hypertrophied astrocytes, as judged by some histochemical findings, showed traits of similarity.