RESUMO
We studied activity and dynamics of apoptosis of peripheral blood lymphocytes in patients with myocardial infarction and analyzed the relationship of these processes with expression of heat shock proteins with a molecular weight of 70 kDa playing an essential role in preventing cell death. Thus, we first demonstrated activation of apoptosis in peripheral blood cells of patients with myocardial infarction compared to the control (healthy individuals) and revealed the expected negative correlation between the expression of heat shock proteins with a molecular weight of 70 kDa by lymphocytes and intensity of their death. The observed dynamics of mononuclear cell apoptosis in the peripheral blood of patients with myocardial infarction can reflect activity of programmed cardiomyocyte death in the focus of ischemic injury.
Assuntos
Apoptose , Proteínas de Choque Térmico/sangue , Linfócitos/citologia , Infarto do Miocárdio/sangue , Miócitos Cardíacos/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Feminino , Proteínas de Choque Térmico/metabolismo , Humanos , Masculino , Pessoa de Meia-Idade , Transdução de SinaisRESUMO
Loading of neutrophils with immune complexes changed their functional activity and intracellular pH. These changes depended on the size of immune complexes. UV irradiation of neutrophils loaded with immune complexes led to a molecular weight-dependent decrease in intracellular pH. After irradiation the chemiluminescence response to latex increased only in neutrophils loaded with non-phagocytized immune complexes with low (less than 10 units IgG) and high molecular weights (more than 25 units IgG).
