Bilayer coatings for extension of the shelf life of fish fillets: Incorporating seaweed sulfated polysaccharides in chitosan-alginate LbL structures.

The aim of this study was to develop a new active coating of layer-by-layer (LbL) structure composed of alginate (as polyanions) and chitosan (as a polycation) containing sulfated polysaccharide (fucoidan) from Sargassum angustifolium, to protect rainbow trout fillets during refrigerated storage. Chitosan and alginate do not combine with each other as a homogeneous solution, so they are suitable for multilayer coatings. The results demonstrated that coating samples with chitosan and fucoidan significantly improved the quality of fish fillets and extended their shelf life from 6 to 16 days. The chemical values (TBARS and TVB-N) and bacterial growth (total viable count (TVC), total psychrophilic count (PTC), and lactic acid bacteria (LAB)) indicated lower levels in the LbL coating samples containing fucoidan compared to the alginate and control samples. Among the different coating samples, the LbL coating with fucoidan (AChF1) exhibited lower weight loss, improved chromaticity (L*, a*, and b*), and minimal changes in mechanical and sensory evaluations. Based on the findings, AChF1 was the most effective treatment for increasing the shelf life of rainbow trout fillets during refrigerated storage. Therefore, it has potential applications in the food packaging industry.

Impact forces in backward falls: Subject-specific video-based rigid body simulation of backward falls.

A critical missing component in the study of real-world falls is the ability to accurately determine impact forces resulting from the fall. Subject-specific rigid body dynamic (RBD) models calibrated to video captured falls can quantify impact forces and provide additional insights into injury risk factors. RBD models were developed based on five backward falls captured on surveillance video in long-term care facilities in British Columbia, Canada. Model joint stiffness and initial velocities were calibrated to match the kinematics of the fall and contact forces were calculated. The effect of joint stiffnesses (neck, lumbar spine, hip, and knee joint) on head contact forces were determined by modifying the calibrated stiffness values ±25%. Fall duration, fall trajectories, and maximum velocities showed a close match between fall events and simulations. The maximum value of pelvic velocity difference between Kinovea (an open-source software 2D digitization software) and Madymo multibody modeling was found to be 6% ± 21.58%. Our results demonstrate that neck and hip stiffness values have a non-significant yet large effect on head contact force (t(3) = 1, p = 0.387 and t(3) = 2, p = 0.138), while lower effects were observed for knee stiffness, and the effect of lumbar spine stiffness was negligible. The subject-specific fall simulations constructed from real world video captured falls allow for direct quantification of force outcomes of falls and may have applications in improving the assessment of fall-induced injury risks and injury prevention methods.