RESUMO
Pegylated hyaluronate-endo-ß-N-acetylhexosaminidase considerably potentiates the hemostimulating effects of erythropoietin due to intensification of proliferation and differentiation of erythroid precursors against the background of enhanced secretion of hemopoietins by nonadherent hemopoiesis-inducing environment cells and elevation of serum erythropoietin concentration. The use of the enzyme allows 10-fold reduction of the maximum effective erythropoietin dose.
Assuntos
Anemia/tratamento farmacológico , Eritropoetina/metabolismo , Eritropoetina/farmacologia , Polietilenoglicóis/metabolismo , beta-N-Acetil-Hexosaminidases/metabolismo , Animais , Carboplatina , Relação Dose-Resposta a Droga , Sinergismo Farmacológico , Ensaio de Imunoadsorção Enzimática , Contagem de Eritrócitos , Eritropoetina/sangue , Feminino , Hemoglobinas/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos CBA , Nanotecnologia/métodos , Proteínas Recombinantes , Estatísticas não ParamétricasRESUMO
This study demonstrated the possibility of in vivo activation of progenitor cells by hyaluronidase. Specifically, treatment with hyaluronidase increased the number of mesenchymal and bone marrow precursor cells, their proliferative activity, and differentiation. Also, it promoted stem cell mobilization into blood under effect of granulocyte colony-stimulating factor (G-CSF) and enhanced progenitor cell adhesive properties. Therapeutic efficiency of transplantation of mononuclear cells isolated from peripheral blood after administration of G-CSF improved under effect of hyaluronidase. Hyaluronidase immobilized by electron beam synthesis nanotechnology exhibited high specific activity with respect to stem cells via both enteral and parenteral routes.
Assuntos
Hialuronoglucosaminidase/farmacologia , Células-Tronco/metabolismo , Animais , Diferenciação Celular/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos CBA , Transplante de Células-Tronco , Células-Tronco/citologia , Células-Tronco/efeitos dos fármacosRESUMO
Mechanisms of action of pantogematogen (PG), granulocyte colony-stimulating factor (G-CSF), glycyram, and D-glucuronic asid (D-GA) have been investigated under the conditions of myelosuppression caused by the introduction of cyclophosphane. It is established that the activation of granulocytopoiesis by these preparations is based on various mechanisms: G-CSF directly stimulates the proliferation and differentiation of hemopoietic cells; PG enhances the proliferation of granulocytes due to activation of the regulatory systems; D-GA and glycyram normalize the structural and functional organization of a bone marrow, thus providing intensive maturing of the colony-forming units.
Assuntos
Antineoplásicos Alquilantes/antagonistas & inibidores , Fatores Biológicos/farmacologia , Ciclofosfamida/antagonistas & inibidores , Granulócitos/efeitos dos fármacos , Leucopoese/efeitos dos fármacos , Animais , Antineoplásicos Alquilantes/toxicidade , Ciclofosfamida/toxicidade , Ácido Glucurônico/farmacologia , Ácido Glicirretínico/farmacologia , Fator Estimulador de Colônias de Granulócitos/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos CBARESUMO
The granulocyte lineage reaction and its mechanism were studied in the bone marrow of patients with breast cancer in stage III-IV treated according to a specific CAF scheme including cropanol, an antitumor drug of the animal origin. It was found that the bone marrow cytopoiesis suppressed by the cytostatics is stimulated by cropanol on the level of committed precursors, morphologically differentiated bone marrow elements, and peripheral blood.
