Distribution of the HLA-DPB1 alleles in a Russian population living in the Chelyabinsk region (South Ural of Russia).

The purpose of this work was to establish the distribution of HLA-DPB1 alleles in Russians living in the Chelyabinsk region (Russia). DPB1 frequencies were determined in 100 unrelated Russian, living in the Chelyabinsk region. All subjects were healthy unrelated blood donors, between 18 and 55 years of age. Typing was performed by NGS (DNA-technology, Moscow). For population genetics analysis GENE[RATE] (https://hla-net.eu/tools/) software was used. DPB1*04:01 prevails in this population (gf = 0.3667). Other frequent HLA-DPB1 alleles were: DPB1*04:02, DPB1*02:01, DPB1*03:01 (gf = 0.1490; 0.1481; 0.1385, respectively). Rare alleles (gf ≤ 0.03) were DPB1*17:01, DPB1*01:01, DPB1*06:01, DPB1*14:01, DPB1*05:01, DPB1*13:01, DPB1*09:01, DPB1*10:01, DPB1*11:01, DPB1*23:01, DPB1*124:01, DPB1*15:01, DPB1*16:01, DPB1*105:01, DPB1*150:01.

The Frequency of CCR5del32 Mutation in Populations of Russians, Tatars and Bashkirs of Chelyabinsk Region, Russia.

The distribution of genetic variants associated with natural resistance to viral infections can vary among human ethnic groups due to evolutionary factors, defining the different epidemiologic background of world populations. The polymorphisms, defining the natural resistance to HIV-infection and the rate of progression up to AIDS, are very important since epidemic is still on rise. We have studied the distribution of allele and genotype frequencies of CCR5delta32 mutation in major populations inhabiting Chelyabinsk region of the Russian Federation. Genetic survey included the population of 509 potential blood marrow donors: Russians (N = 300), Bashkirs (N = 118) and Tatars (N = 91). The genotyping assay was performed using real-time polymerase chain reaction (real-time PCR). The genotypes were defined by melting curve analysis. The CCR5delta32 allele and CCR5delta32/delta32 genotype are presented in population of Russians in Chelyabinsk region with the frequencies of F x  = 10.83% and P x  = 1.67, for the CCR5delta32 allele and its homozygosity, respectively. In populations of Bashkirs and Tatars CCR5delta32 allele and CCR5delta32/delta32 genotype are presented at lower frequencies of F x  = 6.36%/P x  = 0.85 and F x  = 7.14%/P x  = 1.10, respectively. These data are consistent with the theory of northern origin of the CCR5delta32 mutation.