Phylogenetic Relationships of Burbot (Lota lota L., 1758) of the Volga-Kama River Basin Inferred from the Analysis of Mitochondrial DNA Markers.

The phylogenetic relationships of burbot (Lota lota L., 1758) of the Volga-Kama River basin are reconstructed for the first time. The sequences of the gene cytochrome b and the mtDNA control region obtained for 44 samples from the Kama River and Mezhevaya Utka River (the Volga River tributaries) are studied. New haplotypes of both markers were revealed. The results of phylogenetic reconstructions based on cytochrome b and control region mtDNA do not contradict the existing ideas about the phylogenetic structure of the species, and indicate inclusion of burbot from the Volga-Kama basin in Eurasian haplogroup. According to obtained data, the Volga-Kama River basin could play an important role in shaping the genetic diversity of burbot in Europe, and during certain periods it served as a corridor connecting the river systems of the European and Asian parts of the species range.

Isolation of primary osteoblast cell lines from adult rat and rat embryos and their use as models for in vitro biocompatibility tests of nanostructured titanium-based implants.

Methods for obtaining osteoblast cultures from the calvaria of adult Wistar rats and 12-day-old embryos of these rats have been adapted for studying the biocompatibility and ossointegration of titanium-based implants. The osteoblast morphology and their differentiation into osteocytes on a titanium matrix with specially treated surface have been studied. It has been confirmed that two cultures of diploid rat cells obtained in the study can serve as efficient models for preclinical in vitro testing of nanostructured titanium implants for biocompatibility and osseointegration.

First data on genetic diversity of burbot (Lota lota L.) in the Western Siberian.

Analysis of genetic diversity of burbot (Lota lota Linneus, 1758) mitochondrial control region (mtCR) haplotypes from geographically distant localities in the Ob-Irtysh River basin in comparison with distribution of known burbot haplotypes was conducted. mtCR fragments from burbot samples, obtained in two localities (longitudinal part of the Irtysh near Tobolsk and the Sob River, a left-bank tributary of the Ob River), were sequenced.