[Antibody detection in zoonotic cutaneous leishmaniasis by using the indirect immunofluorescence reaction and C-ELISA in different clinical manifestations of the disease]. / Vyiavlenie antitel pri zoonoznom kozhnom leishmanioze s pomoshch'iu RNIF i C-ELISA pri razlichnykh klinicheskikh proiavleniiakh bolezni.

189 sera from patients with ZCL of different stages were studied by means of RNIF and C-ELISA. A high significant correlation between RNIF and C-ELISA indices was shown, but the latter test was proved to be more sensitive and therefore more effective. In patients with ZCL, a significant direct correlation between the duration of the process and the antibody titer was established by means of C-ELISA. In patients with ZCL at the third (final) stage (reduction and granulation of lesions), the C-ELISA liter of specific antibodies was higher than at the second stage (rapidly progressing ulceration of lesions). The findings indicate the presence of clinical and serological parallels in ZCL and show it expedient to compare the dynamics of antibody genesis and cell immunity indices in further studies.

[Immunoenzyme analysis with corpuscular antigen in the study of zoonotic cutaneous leishmaniasis]. / Immunofermentnyi analiz s korpuskuliarnym antigenom pri izuchenii zoonoznogo kozhnogo leishmanioza.

Comparative study of sera from patients suffering from zoonotic cutaneous leishmaniasis by indirect immunofluorescence (IIF) and by enzyme immunoassay (ELISA) gave 72.4 and 89.6% positive results, correspondingly, with homologous antigen L. major. Good correlation of these methods was revealed--coincidence in 83% cases. Authors used ELISA with corpuscular Leishmania antigen. The test was superior to IIF in sensitivity and specificity. Creation of type-specific Leishmania test systems for ELISA opens new vistas in studies of the population immune structure in combined zoonotic cutaneous leishmaniasis and visceral leishmaniasis foci.

[The activization of an Ashkhabad focus of anthroponotic cutaneous leishmaniasis]. / Aktivizatsiia ashkhabadskogo ochaga antroponoznogo kozhnogo leishmanioza.

6 patients with characteristic clinical symptoms of anthroponotic cutaneous leishmaniasis (ACL) have been revealed in 1987-1988 in the city of Ashkhabad (the Turkmen SSR). 3 Leishmania strains isolated from these patients (MHOM/SU/87/392, MHOM/SU/87/393, MHOM/SU/87/410) were identified as ACL causative agents--Leishmania tropica--by means of isoenzymatic analysis. The analysis for 12 enzymes (G-6-PDH, PGM, 6-PGDH, ACP, ALP, PGI, GOT, ME, LDH, ALD, HK, FK) was performed by electrophoresis on acetate-cellulose plates. The entomological studies carried out in Ashkhabad in September 1987 and May 1988 demonstrated the presence of a specific ACL vector--Phlebotomus sergenti. The abundance index of this species was 0.64-0.70. The above-mentioned data indicate reactivization of previously sanitized ACL focus in Ashkhabad (Turkmen SSR).

[Treatment of cutaneous leishmaniasis with methacycline and doxycycline (vibramycin)]. / Terapiia kozhnogo leishmanioza metatsiklinom i doksitsiklinom (vibramitsinom).

Sixty-nine patients suffering from acute necrotic cutaneous leishmaniasis have been effectively treated with methacycline, 60 ones with doxycycline. Many parameters of these drug's efficacies are similar to those of monomycin, the most active drug for cutaneous leishmaniasis. Therapy with the afore-said antibiotics, in combination with methyluracil, an immunocorrecting drug, is a simple and available effective method, that may be used for outpatient treatment of leishmaniasis patients.