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1.
J Clin Virol ; 46(2): 154-60, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19631582

RESUMO

BACKGROUND: Studies have reported differing frequencies of detection of polyomavirus simian virus 40 (SV40) in association with human lymphomas. OBJECTIVE: We addressed the hypothesis that SV40 positivity in lymphomas can vary among sampled populations. STUDY DESIGN: Archival paraffin-embedded lymphoma specimens (n=171) from patients at two urban hospitals in Houston, TX, USA, were analyzed following a cross-sectional study design. Extracted DNAs were characterized by quantitative polymerase chain reaction for the cellular RNase P gene and for SV40 and herpesvirus Epstein-Barr virus (EBV) sequences. RESULTS: Patient characteristics of the two study populations differed significantly whereas the classification of tumor types studied did not. SV40 DNA was detected more frequently in lymphomas from the public hospital population (10/44, 23%) than in lymphomas from the veterans' hospital (VAMC) (4/127, 3%; P<0.0001). EBV detection in lymphomas also differed between the two groups (17/44, 39% vs. 23/127, 18%; P=0.01). SV40 positivity was associated with a younger age category of VAMC lymphoma patients (P=0.02). Expression of T-antigen was detected by immunohistochemistry in half of lymphomas that contained SV40 DNA. Variation was observed in the quality and quantity of DNA recovered from paraffin-embedded specimens, but there was no difference in recoveries of DNA from samples from the two hospitals. CONCLUSIONS: This study demonstrated that, in a direct comparison, the prevalence of SV40 DNA in lymphomas can differ significantly between groups with different demographic distributions.


Assuntos
Infecções por Vírus Epstein-Barr/epidemiologia , Herpesvirus Humano 4/isolamento & purificação , Linfoma/epidemiologia , Linfoma/virologia , Infecções por Polyomavirus/epidemiologia , Vírus 40 dos Símios/isolamento & purificação , Infecções Tumorais por Vírus/epidemiologia , Antígenos Transformantes de Poliomavirus/metabolismo , Estudos Transversais , Infecções por Vírus Epstein-Barr/virologia , Feminino , Herpesvirus Humano 4/genética , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Infecções por Polyomavirus/virologia , Ribonuclease P/genética , Vírus 40 dos Símios/genética , Estatísticas não Paramétricas , Texas/epidemiologia , Infecções Tumorais por Vírus/virologia
2.
J Clin Virol ; 43(1): 66-72, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18539521

RESUMO

BACKGROUND: BK virus (BKV), JC virus (JCV) and simian virus 40 (SV40) are nonenveloped DNA viruses, members of the family Polyomaviridae. BK and JC viruses establish persistent infections in humans, and evidence suggests that SV40 can infect humans, as well. Whether persistence occurs in the lymphoid system is unknown. METHODS: Paraffin-embedded tonsils from 220 immunocompetent children (mean age 9.3 years) were examined by polymerase chain reaction (PCR) to detect viral DNA of BKV, JCV, SV40, and Epstein-Barr virus (EBV). RESULTS: Polyomavirus-specific DNA sequences were detected in 8.3% (29/351) of specimens collected from 220 children. Twenty-one (9.5%) children had polyomavirus DNA present in at least one tonsil, with sequences identified as SV40 (n=20) and BKV (n=1). Polyomavirus JCV was not detected. Among patients positive for SV40, 8 of 14 (57%) contained viral DNA in both available tonsils. EBV DNA was detected in 99 (28.2%) samples from 67 (30.5%) patients. Eleven samples (3.1%) from 8 (3.6%) children were positive for both polyomavirus and EBV. SV40-positive children were significantly older than the SV40-negative subjects (P<0.001). T-antigen expression was detected in an SV40 DNA-positive tonsil by immunohistochemistry. CONCLUSIONS: These results suggest that SV40 can infect tonsils, that lymphoid tissue may represent a site for polyomavirus persistence, and that immunohistochemistry is not a useful detection assay when there are very few virus-infected cells in a tissue.


Assuntos
Tonsila Palatina/virologia , Doenças Faríngeas/diagnóstico , Infecções por Polyomavirus/diagnóstico , Vírus 40 dos Símios/isolamento & purificação , Infecções Tumorais por Vírus/diagnóstico , Adolescente , Antígenos Transformantes de Poliomavirus/genética , Antígenos Transformantes de Poliomavirus/isolamento & purificação , Criança , Pré-Escolar , Feminino , Herpesvirus Humano 4/isolamento & purificação , Humanos , Imunocompetência , Masculino , Tonsila Palatina/patologia , Doenças Faríngeas/virologia , Reação em Cadeia da Polimerase , Infecções por Polyomavirus/virologia , Vírus 40 dos Símios/genética
3.
Arch Pathol Lab Med ; 131(4): 593-8, 2007 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-17425390

RESUMO

CONTEXT: Mucin 4 (MUC4) is a high-molecular-weight membrane-bound glycoprotein that is expressed in the foregut before epithelial differentiation. It is found in normal adult airway epithelium, non-small cell lung carcinoma (NSCLC) and in other human malignancies independent of mucus secretion. Although its tissue distribution has been studied, its utility in predicting prognosis in NSCLC is unknown. OBJECTIVE: To evaluate the relationship between MUC4 overexpression and long-term survival in patients with NSCLC. DESIGN: Immunohistochemical staining for MUC4 was performed on formalin-fixed, paraffin-embedded tissue samples from 343 cases of NSCLC arranged in a high-density tissue microarray. Information about long-term survival and tumor stage was collected for all patients. Semiquantitative assessment of MUC4 staining was correlated with survival (Kaplan-Meier analysis). RESULTS: MUC4 was frequently expressed in adenocarcinomas (151/187 [81%]), squamous cell carcinomas (69/ 88 [78%]), adenosquamous carcinomas (6/8 [75%]), and large cell carcinomas (33/60 [55%]). High levels of expression (combined score, 2+/3+) for MUC4 were more characteristic of adenocarcinomas (126/187 [68%]) and adenosquamous carcinomas (6/8 [75%]) than of squamous cell carcinomas (46/88 [52%]) and large cell carcinomas (17/60 [28%]) (P < .001). In patients with stage I and II adenocarcinoma, there was a trend toward longer patient survival with higher levels of MUC4 immunoreactivity compared with lower levels (P = .11). CONCLUSION: MUC4 expression is common in pulmonary adenocarcinomas and may indicate a more favorable prognosis in early-stage adenocarcinomas.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/patologia , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Mucinas/biossíntese , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/análise , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Pulmonares/metabolismo , Masculino , Pessoa de Meia-Idade , Mucina-4 , Prognóstico , Estudos Retrospectivos , Análise de Sobrevida , Análise Serial de Tecidos
4.
Arch Pathol Lab Med ; 130(6): 823-7, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16740034

RESUMO

CONTEXT: Metastatic renal cell carcinoma (MRCC) involving the thorax can be difficult to distinguish from diffuse malignant mesothelioma (DMM) using traditional morphologic approaches. Standard panels of immunohistochemical markers are of limited benefit. OBJECTIVE: To investigate several antibodies to renal cell carcinoma-associated proteins for differentiating MRCC from DMM. DESIGN: One hundred DMMs and 20 MRCCs were evaluated for immunoexpression of erythropoietin. The same cases and an additional 45 DMMs were evaluated for CD10 and renal cell carcinoma marker (RCCMa) immunoreactivity. RESULTS: Erythropoietin was expressed in 100% of DMMs and MRCCs. Staining for CD10 was observed in 54% of DMMs and 100% of MRCCs. RCCMa stained 26% of DMMs and 55% of MRCCs. Although erythropoietin staining was similarly strong and diffuse in both DMM and MRC, patterns of staining for RCCMa and CD10 differed between MRCC and DMM. Immunoreactivity was strong and diffuse for both RCCMa and CD10 in most MRCCs. Of CD10-positive DMMs, nearly half showed staining in less than 50% of tumor cells and about one fourth of positive cases exhibited only weak to moderately intense staining. Only half of RCCMa-positive DMMs showed staining in more than 49% of tumor cells and staining was only weak to moderately intense in most cases. CONCLUSIONS: Given the overlap in the expression of renal cell carcinoma markers in MRCC and DMM, results with these markers must be interpreted cautiously and should be used in conjunction with mesothelium-associated markers. Differences in expression may potentially help distinguish MRCC from DMM inasmuch as strong and diffuse expression of RCCMa and CD10 supports a diagnosis of MRCC over DMM.


Assuntos
Carcinoma de Células Renais/metabolismo , Eritropoetina/metabolismo , Neoplasias Renais/metabolismo , Mesotelioma/metabolismo , Neprilisina/metabolismo , Neoplasias Pleurais/metabolismo , Biomarcadores Tumorais/metabolismo , Carcinoma de Células Renais/secundário , Diagnóstico Diferencial , Humanos , Técnicas Imunoenzimáticas , Neoplasias Renais/patologia , Mesotelioma/patologia , Neoplasias Pleurais/patologia
5.
Haematologica ; 90(12): 1635-42, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16330436

RESUMO

BACKGROUND AND OBJECTIVES: Simian virus 40 (SV40) is an oncogenic DNA virus implicated in some human malignancies, including lymphomas. In the present masked case-control study, we investigated the prevalence of SV40 sequences and the expression of the viral oncoprotein, large tumor antigen (T-ag), in lymphomas and control specimens from patients negative for the human immunodeficiency virus in Costa Rica. DESIGN AND METHODS: Coded specimens were anlyzed by polymerase chain reaction for SV40 and Epstein-Barr virus (EBV). SV40 sequences were confirmed by Southern blot and DNA sequence analysis. Immunohistochemistry was used to detect the expression of SV40 T-ag in coded samples and to immunophenotype the lymphomas. RESULTS: When samples were decoded, SV40 DNA sequences were detected significantly more often in lymphomas than in control samples (30/125, 24% vs. 0/91, 0%; p=0.001). SV40 DNA was detected in 26% and 10% of non-Hodgkin's and Hodgkin's lymphomas, respectively. EBV DNA was detected in 10% of lymphomas and 33% of control specimens. None of the lymphomas was positive for both SV40 and EBV. Expression of SV40 T-ag was detected in 64% of B-cell lymphomas that contained T-ag DNA sequences and in none of the samples negative for viral DNA. Not all cells in a positive tumor expressed T-ag and the reactions were relatively low intensity. A germinal center B-cell-like profile was frequently associated with SV40-positive lymphomas. Of note, 20% of patients with SV40-related lymphomas were born in the 1970s and 1980s. INTERPRETATION AND CONCLUSIONS: These results indicate that SV40 is significantly associated with some B-cell neoplasms in Costa Rica today.


Assuntos
Antígenos Transformantes de Poliomavirus/análise , Linfoma/virologia , Vacina Antipólio de Vírus Inativado/efeitos adversos , Infecções por Polyomavirus/epidemiologia , Vírus 40 dos Símios/patogenicidade , Infecções Tumorais por Vírus/epidemiologia , Adulto , Estudos de Casos e Controles , Comorbidade , Costa Rica/epidemiologia , DNA Viral/análise , Contaminação de Medicamentos , Infecções por Vírus Epstein-Barr/epidemiologia , Feminino , Centro Germinativo/virologia , Soronegatividade para HIV , Herpesvirus Humano 4/isolamento & purificação , Herpesvirus Humano 4/patogenicidade , Doença de Hodgkin/epidemiologia , Doença de Hodgkin/virologia , Humanos , Neoplasias Hepáticas/epidemiologia , Neoplasias Hepáticas/virologia , Linfonodos/virologia , Linfoma/epidemiologia , Linfoma de Células B/epidemiologia , Linfoma de Células B/virologia , Linfoma não Hodgkin/epidemiologia , Linfoma não Hodgkin/virologia , Masculino , Pessoa de Meia-Idade , Tonsila Palatina/virologia , Infecções por Polyomavirus/virologia , Pseudolinfoma/epidemiologia , Pseudolinfoma/virologia , Vírus 40 dos Símios/isolamento & purificação , Neoplasias Gástricas/epidemiologia , Neoplasias Gástricas/virologia , Infecções Tumorais por Vírus/virologia
6.
Virology ; 342(1): 38-46, 2005 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-16122775

RESUMO

Simian virus 40 (SV40) is associated with some systemic non-Hodgkin's lymphomas (NHL) among HIV-positive patients, based on assays for viral DNA sequences. To investigate the possible production of the viral transforming protein, we examined age-matched case-control specimens from patients with HIV/AIDS for the expression of SV40 large tumor antigen (T-ag). Masked specimens initially examined by polymerase chain reaction (PCR) for polyomavirus and herpesvirus DNA sequences were assessed for the expression of SV40 T-ag and phenotypic lymphocyte markers by immunohistochemistry (IHC). Fifty-five systemic NHL and 25 nonmalignant lymphoid and malignant nonlymphoid tissue control cases from two HIV community programs in Texas and New Jersey were scored for IHC positivity without knowledge of the PCR results. IHC showed expression of SV40 T-ag among B-cell lymphomas, whereas none of the control tissue samples were positive for T-ag (12/55, 22% vs. 0/25, 0%; P = 0.01). SV40 T-ag expression was detected only in B-cell lymphoma specimens that contained SV40 DNA sequences. Not all lymphoma cells in a positive specimen stained for T-ag, and the reaction was lower intensity than observed in SV40 hamster tumors. SV40 T-ag was demonstrated in both primary and recurrent tumors from one patient. A germinal center B-cell-like (GCB) profile was more frequently expressed by SV40-positive tumors than in Epstein-Barr virus (EBV)-related lymphomas (10/12, 83% vs. 6/13, 46%; P = 0.05), whereas a non-GCB phenotype was more frequent in EBV-positive than in SV40-positive lymphomas (7/13, 54% vs. 2/12, 17%; P = 0.05). This study shows that SV40 gene expression occurs in a fraction of cells in some B-cell lymphomas among patients with HIV/AIDS.


Assuntos
Antígenos Transformantes de Poliomavirus/genética , Linfoma Relacionado a AIDS/imunologia , Linfoma Relacionado a AIDS/virologia , Linfoma não Hodgkin/imunologia , Linfoma não Hodgkin/virologia , Adulto , Sequência de Bases , Estudos de Casos e Controles , DNA Viral/genética , DNA Viral/isolamento & purificação , Feminino , Expressão Gênica , Genes Virais , HIV-1 , Humanos , Imunofenotipagem , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Recidiva Local de Neoplasia/imunologia , Recidiva Local de Neoplasia/virologia , Vírus 40 dos Símios/genética , Vírus 40 dos Símios/isolamento & purificação
7.
Virology ; 330(1): 168-77, 2004 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-15527843

RESUMO

Different strains of simian virus 40 (SV40) exist and are associated with some human malignancies, but it is not known if SV40 strains differ in biological potential in vivo. In two long-term experiments, Syrian golden hamsters 21 days of age were inoculated by the intraperitoneal route with two different strains of SV40 (10(7) plaque-forming units/animal) and were followed for 8 or 12 months. In vivo responses to strain VA45-54, isolated originally from monkey kidney cells, and to strain SVCPC, recovered from human cancers, were compared. Control animals of the same age were inoculated intraperitoneally with cell culture media. Malignancies developed only in animals infected with SV40 and not in controls. The rate of tumor development was more frequent among animals infected with strain SVCPC than with VA45-54, both in experiments held for 8 months (11/22, 50% vs. 4/20, 20%) and for 12 months (7/15, 47% vs. 3/13, 23%). Histologically, the tumors resembled mesotheliomas, osteosarcoma, and poorly differentiated sarcomas. Metastases to lung and lymph nodes occurred with both viral strains. T-antigen expression was detected in most tumor cells by immunohistochemistry. Anti-T-antigen antibodies were produced by almost all tumor-bearing animals and by about two-thirds of those that did not develop tumors after virus inoculation. SV40 viral neutralizing antibodies were detected in all tumor-bearing animals and in 92% and 38% of those inoculated with SVCPC and VA45-54, respectively, that failed to develop tumors. Antibody titers were usually higher in animals with tumors than in those without. Control animals did not develop viral antibodies. Infectious virus was recovered from 2 of 15 tumors tested. This study showed that there are biological differences between these two SV40 strains that influence the outcome of infections in normal hosts, including the development of malignancies and neutralizing antibody, and proved the principle that SV40 strains from different clades can vary in biological properties in vivo.


Assuntos
Neoplasias Experimentais/patologia , Neoplasias Experimentais/virologia , Vírus 40 dos Símios/patogenicidade , Animais , Cricetinae , Humanos , Mesocricetus , Vírus 40 dos Símios/classificação , Especificidade da Espécie , Ensaio de Placa Viral
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