Synthesis and Characterization of Carboxymethyl Chitosan Scaffolds Grafted with Waterborne Polyurethane.

Bone tissue engineering has been rapidly developed in regenerative medicine field, which aims to induce new functional bone regeneration through the synergistic combination of biomaterials and cells. Porous biomaterials with sufficient mechanical properties and functional impregnating for bone substitutes have been imposed in the oncoming generation of bone reconstruction. In this study, we fabricated Carboxymethyl chitosan three dimensional (3D) porous scaffold modified with waterborne polyurethane (WPU) through freeze drying technique. In order to check its potential in bone tissue substitutes, osteoblast cells (hFOB 1.19) were seeded onto the fabricated scaffolds and then, SEM and proliferation assay were performed. The enhanced proliferation was contributed to 3D macroporous network structure, large surface area, and osteoconductive environments.

CCN3 overexpression inhibits growth of callosal projections via upregulation of RAB25.

The cysteine-rich 61/connective tissue growth factor 3 (CCN3) is a member of the CCN family of secreted multifunctional proteins involved in a variety of cellular processes including migration, adhesion, and differentiation. Previous studies have shown that CCN3 is expressed in the developing rat central nervous system, and enhanced CCN3 expression is highly correlated with tumorigenesis. However, the expression pattern and influence of abnormal CCN3 expression during mouse cortical development remains to be elucidated. Here, we show that CCN3 expression in mice is first detectable at embryonic day 15 and increases until postnatal day 21. We overexpressed CCN3 in mouse cortical neurons using uni- and bilateral electroporation. Our in vivo overexpression experiments showed that elevated CCN3 expression inhibited the axonal outgrowth of callosal projection neurons. Moreover, we identified the small GTPase RAB25 as a downstream effector molecule of CCN3 using transcriptomic analysis with CCN3 overexpressed in cortical tissue. In vivo ectopic expression of RAB25 or the dominant-negative RAB25-T26N also revealed that the GTPase activity of RAB25 is involved in the CCN3-mediated regulation of neuronal outgrowth. Taken together, our results suggest that tight regulation of CCN3 expression is necessary for normal cortical neuronal connectivity during development, and RAB25 negatively regulates neuronal differentiation as a downstream effector of CCN3.

Radioactivity evaluation for the KSTAR tokamak.

The deuterium-deuterium (D-D) reaction in the KSTAR (Korea Superconducting Tokamak Advanced Research) tokamak generates neutrons with a peak yield of 2.5 x 10(16) s(-1) through a pulse operation of 300 s. Since the structure material of the tokamak is irradiated with neutrons, this environment will restrict work around and inside the tokamak from a radiation protection physics point of view after shutdown. Identification of neutron-produced radionuclides and evaluation of absorbed dose in the structure material are needed to develop a guiding principle for radiation protection. The activation level was evaluated by MCNP4C2 and an inventory code, FISPACT. The absorbed dose in the working area decreased by 4.26 x 10(-4) mrem h(-1) in the inner vessel 1.5 d after shutdown. Furthermore, tritium strongly contributes to the contamination in the graphite tile. The amount of tritium produced by neutrons was 3.03 x 10(6) Bq kg(-1) in the carbon graphite of a plasma-facing wall.