RESUMO
Dysbiotic shifts in intestinal and pharyngeal microflora were studied in 22 normal volunteers in 9-, 14-, 105- and 520-d chamber experiments simulating some of the spaceflight factors. Two preparations were administered to prevent pharyngeal and intestinal dysbiosis: oral dry probiotic based on indigenous intestinal Enterococci and topical collagen-immobilized Lactobacterin based on indigenous intestinal Lactobacilli. Topical autopmrobiotic lactobacterin reduced the growth of opportunistic pathogens in the throat during the experiments. Oral autoprobiotic based on Enterococci reduced the content of intestinal opportunistic pathogens, supporting the high level of protecting microflora. Most of autostrains are free from pathogenicity factors, nevertheless, implementation of genetic testing of indigenous strains are reasonable.
Assuntos
Bacteriocinas/farmacologia , Enterococcus faecium/genética , Inflamação/prevenção & controle , Intestinos/microbiologia , Probióticos/farmacologia , Adulto , Bacteriocinas/química , Células Imobilizadas , Colágeno , Enterococcus , Enterococcus faecium/isolamento & purificação , Meio Ambiente Extraterreno , Humanos , Intestinos/efeitos dos fármacos , Lactobacillus , Masculino , Doenças Faríngeas/prevenção & controle , Faringe/efeitos dos fármacos , Faringe/microbiologia , Voo EspacialRESUMO
Daily dynamics of pharyngeal microflora was evaluated in the absence of preventive treatment and using a local lactate-producing probiotic preparation during 24 hour-long simulated microgravity. The study showed that the short-term application of the local lactate-producing probiotic (four times daily) significantly reduced titers of opportunistic pathogenic microflora and increased the amount of symbionts in the pharynx.
Assuntos
Lactatos/farmacologia , Lactobacillus , Faringe/microbiologia , Probióticos/farmacologia , Adolescente , Adulto , Humanos , Faringite/tratamento farmacológico , Faringite/microbiologia , Valores de Referência , Ausência de Peso , Adulto JovemRESUMO
Dysbiotic shifts in intestinal and integumentary microflora were studied in 10 test-subjectes during 7-day "dry" immersion. Essentially every test-subject reduced significantly fecal lactoflora and developed dysbiotic shifts in the microbial landscape of various types of integument. Pharyngeal microflora was analyzed in 22 normal volunteers for 7-day "dry" immersion. Two probiotic preparations were administered to prevent pharyngeal dysbiosis: peroral dry lactobacterin and topical collagen-immobilized lactobacterin. The peroral probiotic stimulated growth of pharyngeal opportunistic pathogens preventing translocations of the intestinal microflora. The topical probiotic, on the opposite, reduced the content of opportunistic pathogens in the throat; however, it provoked gastrointestinal dysbiosis. It appears that the most effective prophylaxis of pharyngeal dysbiosis can be reached by prescription of both topical and peroral probiotics.
Assuntos
Infecções Bacterianas/prevenção & controle , Imersão/efeitos adversos , Intestinos/microbiologia , Probióticos/uso terapêutico , Adolescente , Adulto , Anti-Infecciosos/uso terapêutico , Infecções Bacterianas/etiologia , Bacteriocinas/uso terapêutico , Seguimentos , Humanos , Lactobacillus/isolamento & purificação , Valores de Referência , Resultado do Tratamento , Adulto JovemRESUMO
We studied pharyngeal microflora in 22 healthy volunteers after 7-day dry immersion. For prophylactic pharyngeal dysbiosis, we used two probiotic drugs: oral -- "lactobacterin dry" and local --"lactobacterin immobilized on collagen". Administration of oral probiotic was accompanied with growth of pharyngeal opportunistic microflora preventing translocation of intestinal microflora. Local probiotic, on the contrary, decreased opportunistic microflora in the pharynx, but was associated with gastrointestinal dysbiosis. It is evident that combined use of topic and oral probiotic provides maximal effective prophylaxis of pharyngeal dysbiosis.