RESUMO
AIM: Development of methodological approaches for identification of leptospira by using MALDI-TOF direct protein profiling technology. MATERIALS AND METHODS: Analysis of cell proteins of 34 leptospira strains was carried out in Microflex LT by using "MALDI Biotyper 3.0 for identification and classification of microorganisms" program. RESULTS: 19 reference spectra of reference leptospira strains from 7 species were generated and imported into MALDI Biotyper 3.0 database. Identification of 6 strains with undetermined taxonomic position was carried out. CONCLUSION: The approved method allows determination of leptospira species with accuracy that depends on their adaptation to nutrient media, preparation approach and sample storage conditions for mass-spectrometry.
Assuntos
Proteínas de Bactérias/genética , Leptospira/genética , Leptospirose/genética , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Proteínas de Bactérias/isolamento & purificação , Humanos , Leptospira/isolamento & purificação , Leptospira/patogenicidade , Leptospirose/microbiologiaRESUMO
Multi-year literature data as well as materials of the Reference centre of monitoring of natural foci infections of Irkutsk Research Institute of Plague Control of Siberia and the Far East regarding epidemiology, epizootology and laboratory diagnostics of leptospiroses in Siberia and the Far East are analyzed and summarized in the review. Situation on leptospiroses in the region has changed significantly. In 50 - 70s of the 20th century diseases were registered ubiquitously in the form of outbreaks, group and single cases. Currently a low level of sporadic morbidity is noted in separate subjects of the Russian Federation. The contemporary state of the problem remains insufficiently clear, this demands the expansion of studies, creation of modern databases, as well as introduction into the practice of highly sensitive methods of express diagnostics in a complex with bacteriologic and serologic methods.
