1.
Angew Chem Int Ed Engl
; 52(40): 10593-7, 2013 Sep 27.
Artigo
em Inglês
| MEDLINE
| ID: mdl-23960025
RESUMO
A proteomics method to pull down secondary drug targets from live cells is described. The drug of interest is modified with trans-cyclooctene (TCO) and incubated with live cells. Upon cell lysis, the modified drug bound to the protein is pulled down using magnetic beads decorated with a cleavable tetrazine-modified linker. Samples are then run on an SDS-PAGE gel and isolated bands are submitted for mass spectrometry analysis to identify drug targets.