RESUMO
Benzyl benzoate (BB), one of the benzyl derivates, is a component of brown aromatic resin in cinnamon oil and cough syrups and it is widely used in various fields in the perfume, pharmaceutical, and food industries. It is absorbed and hydrolyzed to benzoic acid and benzyl alcohol. Two different doses of BB (25 mg kg-1 body weight and 100 mg kg-1 body weight) were orally administered to 5-week old male rats for 90 days. Histopathological, morphological, hematological, and biochemical assays were performed in toxicological evaluations. Initial/final body weights, relative organ weights, and food and water consumptions of rats did not change significantly. There were statistically significant differences in terms of monocyte, neutrophil, lymphocyte %, and serum AST levels in control and BB treatment groups. Several histopathological findings were observed in liver, kidney, thymus, prostate, and epididymis tissues of the rats in the treatment groups. Immunohistochemical examinations were also performed in the tissues for fibronectin (FN), type IV collagen, transforming growth factor ß (TGF-ß), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of metalloproteinase-2 (TIMP-2). Alterations in immunolocalization of these markers were observed between the control and the treatment groups. No changes were detected in the sperm count, daily sperm production, and sperm morphology.
Assuntos
Benzoatos , Animais , Benzoatos/toxicidade , Peso Corporal , Aditivos Alimentares , Masculino , Metaloproteinase 2 da Matriz , Preparações Farmacêuticas , Ratos , Inibidor Tecidual de Metaloproteinase-2Assuntos
Tecido Adiposo/metabolismo , Cerâmica/química , Materiais Revestidos Biocompatíveis/metabolismo , Adesivo Tecidual de Fibrina/metabolismo , Fibronectinas/metabolismo , Hidroxiapatitas/química , Osteogênese/efeitos dos fármacos , Fosfatase Alcalina/metabolismo , Proteína Morfogenética Óssea 2/genética , Proteína Morfogenética Óssea 2/metabolismo , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Subunidade alfa 1 de Fator de Ligação ao Core/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Propriedades de SuperfícieRESUMO
Celecoxib is intended for acute pain, menstrual cramps, pain, and inflammation of osteoarthritis and rheumatoid arthritis. The aim of this study was to evaluate the effects of celecoxib (10 and 50 mg/kg/day) treatment on rats orally for 28 days. We examined effects on some biochemical parameters and kidney and liver tissues of celecoxib-treated Wistar albino male rats. At the end of the study, hepatic and renal function tests were performed and liver and kidney of rats were microscopically examined to detect systemic toxicity of celecoxib. Celecoxib-treated rats had statistically significant decreases of cholesterol, total bilirubin, total protein, urea, globulin, blood urea nitrogen, phosphorus, and calcium. Serum gamma glutamyl transferase levels increased in 10- and 50-mg/kg/day celecoxib-treated rats. Histological examinations showed mononuclear cell infiltration, hyperplasia, and cellular degeneration in liver and tubular damage and mononuclear cell infiltration in kidney. We suggest that high doses of celecoxib may cause changes in liver and kidney histopathology, liver function, and in some biochemical parameters.
