RESUMO
Mice were injected with poly-4-vinylpyridine at a dose known to be effective for immunogenesis stimulation. Within 24 hours after the injection fibroblasts reacted by dendrit production, cytoplasm vacuolization and clasmocytosis. 2-3 days after the polymer injection the fibroblast cytoplasm was restored. The immunofluorescence employed rabbit serum natural antibodies to fibroblast antigens. The reaction observed may be used for the estimation of biological polymer activity.
Assuntos
Tecido Conjuntivo/efeitos dos fármacos , Polivinil/farmacologia , Animais , Células do Tecido Conjuntivo , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Imunofluorescência , Camundongos , Camundongos Endogâmicos CBARESUMO
It has been demonstrated by the indirect immunofluorescent method that sera of rabbits immunized with nontype-specific (NTS) proteins of the group A streptococcal cell wall react with cultivated fibroblasts. These fibroblasts were explanted from human and guinea-pig lymphoid organs. The same reactions were observed with F(ab')2 fragments of IgG obtained from antisera to NTS-proteins. Fractions containing NTS-antigens inhibited these reactions. A possible role of the cross-reactive streptococcal antigen in common with the antigen of lymphoid organs during thymic damage and the development of the autoimmune process in rheumatic fever is discussed.
Assuntos
Antígenos de Bactérias/análise , Antígenos de Neoplasias , Antígenos/análise , Moléculas de Adesão Celular , Tecido Linfoide/imunologia , Streptococcus pyogenes/imunologia , Animais , Células Cultivadas , Reações Cruzadas , Fibroblastos/imunologia , Imunofluorescência , Glicoproteínas/análise , Cobaias , Humanos , Fragmentos Fab das Imunoglobulinas/análiseRESUMO
Sera from rabbits immunized with synthetic poly-L-alanine (PL-ala) in complete Freund's adjuvant (CFA) have been examined by the indirect immunofluorescence (IF) technique. Half of the sera reacted with fibroblasts of the interstitial connective tissue of the myocardium. The reactions were obtained with all sections of the myocardium of man and other mammals except rabbits. The sera obtained before or after immunization with synthetic poly-L-lysine fibroblasts were obtained with preparations of IgG or F(ab')2 fragments of IgG isolated from sera of animals immunized synthetic polypeptides can be applied to study by the IF technique of the antigenic structure of tissues including lymphoid and tumor tissues.
Assuntos
Soros Imunes/imunologia , Miocárdio/imunologia , Peptídeos/imunologia , Animais , Vacina BCG/imunologia , Fibroblastos/imunologia , Imunofluorescência , Adjuvante de Freund/administração & dosagem , Fragmentos Fab das Imunoglobulinas/imunologia , Imunoglobulina G/imunologia , Miocárdio/citologia , Coelhos/imunologia , Receptores Fc/imunologiaRESUMO
The data are confirmed about the presence of Fc-receptors reacting with human and rabbit IgG on the fibroblasts of human and bovine heart valves. It is shown the similar receptors are present on the fibroblasts of joints and L-cells. The receptors react largely with monomeric IgG. Clear-cut distinctions are revealed between the fibroblasts of heart valves and those of the myocardium which like other cells of myocardial interstitial connective tissue do not contain Fc-receptors.
Assuntos
Valvas Cardíacas/imunologia , Receptores Fc/isolamento & purificação , Animais , Bovinos , Fibroblastos/imunologia , Humanos , Fragmentos Fab das Imunoglobulinas/imunologia , Fragmentos Fc das Imunoglobulinas/imunologia , Imunoglobulina G/imunologia , Técnicas In Vitro , Articulações/imunologia , Células L/imunologia , Masculino , Camundongos , Miocárdio/imunologia , CoelhosRESUMO
It has been revealed by indirect immunofluorescence that sera of rabbits immunized with group A streptococcal non-type-specific antigens and F (ab')2 fragments of IgG obtained from these sera intensively react with fibroblasts of interstitial connective tissue (ICT) of the myocardium of humans and different mammalian species with the exception of rabbits. Negative or weak reactions with the same sera and F (ab')2 fragments were observed with fibroblasts of ICT of nonimmunized rabbits. Myocardial ICT of immunized animals showed bound immunoglobulins. This model can be used for making clear the reason for presence in sera of rheumatic patients circulating antibodies only to heterologous but not to homologous ICT of the myocardium regardless of the revealing bound immunoglobulins in the ICT of these patients.
Assuntos
Reações Cruzadas , Miocárdio/imunologia , Streptococcus pyogenes/imunologia , Animais , Antígenos de Bactérias/imunologia , Autoanticorpos/análise , Bovinos , Fibroblastos/imunologia , Imunofluorescência , Cobaias , Coração/embriologia , Humanos , Fragmentos Fab das Imunoglobulinas/imunologia , Miocárdio/citologia , Coelhos/imunologia , Ratos , Febre Reumática/imunologia , Especificidade da Espécie , Suínos/imunologiaRESUMO
A spectrophotometric method for determination of levomycetin in "Levovinysol", an antiburn aerosol preparation was developed. The determination was performed after levomycetin extraction with a hydrochloric acid solution from the composition. The results of the determination were statistically treated.
Assuntos
Cloranfenicol/análise , Aerossóis , Combinação de Medicamentos , Estabilidade de Medicamentos , Soluções , EspectrofotometriaRESUMO
A colorimetric method for guantitative determination of neomycin in the aerozol preparation "Neotizol" was developed. The determination was performed after neomycin isolation from the film-forming composition with the carboxylic ion exchange resins IRC-50 and KB-2 containing 2.5-3 per cent of divinylbenzol. The results of the colorimetric determination were compared with the data of the microbiological assay. The results of neomycin determination in the aerozol preparation "Neotizol" were treated statistically.