[Effects of a fixed mixture of lactate, glucose and phosphate on the survival of human spermatozoa]. / Effet d'un mélange déterminé de lactate, glucose et phosphate sur la survie des spermatozoïdes humains.

It is admitted that selection of human motile spermatozoa improves chances of conception by homologous artificial insemination. Effect of media constituted by different concentrations of glucose, lactate and phosphate in the maintenance of vitality and forward motility (positively correlated with penetrating capacity) was studied in human spermatozoa after washing. Lactate optimal concentrations appear necessary in the maintenance of progressive motility. The vitality is little affected in the various media. To complete this minimum medium various substances known to improve spermatozoa survival should be added. This complete media should allow to dispose of suitable spermatozoa suspensions during several days permitting many inseminations by cycle.

[Survival of motile spermatozoa in a medium based on lactate, glucose and phosphate]. / Survie des spermatozoïdes mobiles dans un milieu à base de lactate, glucose et phosphate.

Suspensions of motile human spermatozoa, plasma seminal-free, were incubated several days into synthetic medium (C2) with lactate, glucose and phosphate as principal components. Maintenance of forward motility was compared in C2 medium and in known B2 I.N.R.A. medium. C2 medium entertain survival of motile spermatozoa during some days at 20 degrees C. This method of conservation should allow many homologous artificial inseminations by ovulatory cycle avoiding injurious effects of repeated ejaculations or low-temperature conservation.

[Histo-enzymatic activities of the peritoneal serosa covering the splenic capsule in various rodents]. / Activities histo-enzymatiques de la sereuse peritoneale recouvrant la capsule splenique chez quelques rongeurs

The peritoneal cells of the splenic capsule have no phosphatasic acid acitivity in the three species studied. However they have an ATPasic acitivity in the Rat and the Rabbit's spleen, and a 5'nucleotidasic activity in the Guinea-Pig. These cells are not phagocytic and probably have different origins according to the species.

[The effect of cortisone on lympho-reticular cells with specific enzyme activity of the white pulp of rat spleen]. / Les particularités de l'action cortisonique sur des éléments lympho-réticulaires à activité enzymatique spécifique de la pulpe blanche splénique du rat

By its destructive effect on the small lymphocyte, cortisone was used to separate the T-lymphocyte from other cells which might be found in the same areas. A reticular cell showing a predominant 5' nucleotidasic activity distinct from that of the phosphatasic acid appeared to be a component of the thymus-dependent areas in the splenic white pulp of Rat. Specific correlations seem to exist between this reticular cell and T-lymphocytes. This histoenzymatic feature involves the reticular cell in the process of maturation of the small lymphocyte.

[Changes induced with cyclophosphamide in the lympho-reticular elements with specific enzymatic activities of the splenic white pulp in rats and guinea-pigs]. / Altérations provoquées par l'action du cyclophosphamide sur les elements lympho-réticulaires à activités enzymatiques spécifiques de la pulpe blanche splénique du rat et du cobaye

The effect of cyclophosphamide, one potent immunosupressor drug, was used to differenciate the behaviour of two types of Reticular cells, the phosphatasic acid and the 5'nucleotidasic cells. The first exhibit macrophagic and migratory properties, the second are devoid of these criteria but remain associated with the presence of T-lymphocytes. This suggests its possible role in the nutrition and the differential evolution of T-lymphocytes.

[Comparison of topographical locations of reticular cells with 5'-nucleotidase activity in spleen white pulp and lymph nodes in man and rat]. / Comparison des localisations topographiques des cellules réticulaires à activité 5'-nucléotidasique dans la pulpe blanche splénique et les ganglions lymphatiques humains et du rat

The cells connections occuring in thymus-dependent areas of the splenic white pulp of rat between 5'nucleotidasic reticular cells and small lymphocytes allowed to research this same associated cells in the rat lymph node and the human splenic white pulp and lymph node. The locations of the thymus- dependent areas seem different in man and rat.