RESUMO
Natural textures (networks) are observed in many cases: the inter-cellular contact sites, endoplasmic reticulum membranes etc. The vast amount of experimental data was analyzed to produce the distribution histograms for the length of the segments in the protein and chromatin networks of different origin. The networks both from the eukaryotic cells and nucleis, as well as from E. coli and viruses are presented. Statistical analysis demonstrated that all experimentally observed histograms fit to the following formula: F(x) = (5(5)[4]) x x(4)x exp(-5x) where x =l/
Assuntos
Cromatina/metabolismo , Hepatócitos/fisiologia , Interfase/fisiologia , Modelos Biológicos , Animais , Cromatina/ultraestrutura , Biologia Computacional , Hepatócitos/ultraestrutura , Microscopia Eletrônica de Transmissão , RatosRESUMO
The effect of some steroid hormones and their synthetic analogs--dexamethasone, estradiol, progesterone, testosterone, and cytostatics--on the fibroblast nuclei in a monolayer culture was studied. Their proliferation was studied at the same time by the radioindicator method. The method of computerized morphodensitometry with the use of the apparatus-programmer complex DiaMorf (Russia) showed that the compounds under study induce reorganization of interphase chromatin of fibroblast nuclei over the molecules, the amount of which may be recorded by means of the computer system of analysis of cell and tissue images.
Assuntos
Antineoplásicos/farmacologia , Técnicas Citológicas/instrumentação , Células L/efeitos dos fármacos , Microcomputadores , Esteroides/farmacologia , Algoritmos , Animais , Células Cultivadas , Técnicas Citológicas/estatística & dados numéricos , Densitometria/instrumentação , Densitometria/estatística & dados numéricos , Desenho de Equipamento , CamundongosRESUMO
Mycoplasma hominis was incubated with HeLa cells up to 72 h. Infection of HeLa cells was estimated by in situ polymerase chain reaction with a subsequent use of computer digital image analysis for result processing. The localization of mycoplasma in the cell nucleus was demonstrated after a 48 h incubation. The quantity of infected HeLa cells was increased with time to make 58 and 75% on days 2 and 3, respectively. No M. hominis was found in the cytoplasma of HeLa cells.
Assuntos
Células HeLa/metabolismo , Mycoplasma hominis/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Humanos , Sensibilidade e EspecificidadeRESUMO
Technologies based on the use of polymerase chain reaction, in particular IS PCR are widely used now. However, wide introduction in clinico-laboratory practice of these methods requires: increase of reliability of the accepted decisions, improvement of the image for revealing more fine distinctions of objects, increase of quality of the obtained information, automated registration of results of the analysis. The application of original digital image analysis, adapted to specificity of biomedical objects--morphodensitometry--allows to solve successfully the following tasks: to optimisze conditions of IS PCR realisation on the basis of use of quantitative opticogeometrical parameters, quantitatively and subpopulistically to characterize a degree of infected cells with viral infection, and also nucleuses of cells, that is especially actual for introduction of molecular-genetic methods in clinico diagnostic practice and, automatically to register results of the analysis. Thus, employment of morphodensitometry can be very important in genediagnostic and also gene therapy.
Assuntos
Processamento de Imagem Assistida por Computador , Marcação in Situ com Primers/métodos , Animais , Humanos , Células Tumorais CultivadasAssuntos
Exposição Ambiental/efeitos adversos , Eritrócitos/efeitos da radiação , Centrais Elétricas , Liberação Nociva de Radioativos , Tamanho Celular , Criança , Densitometria , Eritrócitos/patologia , Humanos , Processamento de Imagem Assistida por Computador , Doses de Radiação , Federação Russa , UcrâniaRESUMO
Modification of poly(G).poly(C) with cys-diaminodichloroplatinum (cys-DDP) at the level of rb = 0.02 increased the in vivo antiviral and interferon-inducing activity of the complex, in contrast to the data reported for complex poly(G).poly(C). Antiinfluenza activity in this case depends on the method of modification and increases more intensively when a ready complex is treated with cys-DDP, as against treatment of poly(G) alone before the formation of a complex with poly(C). If rb is increased, the activity reduces again. Modification with trans-DDP at rb = 0.02 also leads to an increase of antiinfluenza activity of poly(G).poly(C), but mainly after pretreatment of poly(G).
Assuntos
Antivirais/farmacologia , Cisplatino/química , Vírus da Influenza A/efeitos dos fármacos , Indutores de Interferon/farmacologia , Poli C/farmacologia , Poli G/farmacologia , Animais , Antivirais/química , Indutores de Interferon/química , Camundongos , Poli C/química , Poli G/químicaRESUMO
The paper deals with the automatic morphodensitometric analysis of various biomedical objects (such as interphase chromatin in the cellular nuclei of chondroblasts and lymphocytes or human peripheral blood, opacities in the lens of chick embryos. A complex algorhythm of computational image processing was created for submolecular restructuring of interphase chromatin cell nuclear examinations. It includes specific methods of staining and unifies different quantitative analytical methods: photometrical, geometrical, gradiental, informational, and statistical. The blood cells of the children who had received low-dose radiation were detected by employing a new method of TV image computational processing of DNA-stained lymphocytes. The approach demonstrated by this study provides a sensitive and meaningful mean for diagnosing human diseases and can be useful in the clinical setting.
Assuntos
Densitometria , Processamento de Imagem Assistida por Computador , Tomografia Computadorizada por Raios X , Adolescente , Adulto , Algoritmos , Animais , Cartilagem/citologia , Catarata/diagnóstico , Catarata/patologia , Embrião de Galinha , Criança , Pré-Escolar , Cromatina/ultraestrutura , DNA/análise , Humanos , Interfase , Linfócitos/citologia , Linfócitos/efeitos da radiação , Fotometria , Doses de Radiação , Coloração e RotulagemRESUMO
Proceeding from published data on lipid content and composition in human skin and on the possible relationship between cholesterol level in skin biopsy specimens and status of blood vessels, the authors measured cholesterol content in skin layers available for external extraction in patients with atherosclerosis with different types of dyslipoproteinemias. Effective conditions of extraction were developed permitting after external rapid atraumatic treatment extraction of the maximally possible amount of cholesterol, 1.5 to 1.6 mkg from 1 cm2 of the palm skin surface, this being a constant value for each examinee (coefficient of variations after 3-4 repetitions 5%). Though slight, but statistically reliable increase of the amount of surface-extractable cholesterol was observed in atherosclerosis patients. This parameter did not correlate with the blood serum concentration of lipid components. A correlation (r = 0.54, p < 0.001) was observed with an only serum lipoprotein parameter, the so-called dyslipoproteinemia coefficient representing the sum of "atherogenic" parameters (cholesterol+triglycerides) divided by alpha-lipoprotein cholesterol. Measurements of surface-extractable skin cholesterol may be used as an available discriminant of atherosclerosis; the method seems to find application in other diseases as well.
Assuntos
Arteriosclerose/diagnóstico , Colesterol/análise , Pele/química , Arteriosclerose/sangue , Arteriosclerose/metabolismo , Fenômenos Bioquímicos , Bioquímica , Colesterol/sangue , Colesterol/isolamento & purificação , Epiderme/química , Humanos , Pele/metabolismo , Propriedades de SuperfícieRESUMO
The use of a computer--based morphometric complex "DIAMORPH" for automated morphometric analysis of renal glomeruli is described. By combining the principles of morphological and functional approaches to diagnosis with the capabilities of present--day research equipment, a new informative morphometric parameter--the extent of capillary--mesangium contact--has been identified. With this parameter and its derivatives, it has been possible to differentiate, in a statistically significant way (p less than 0.01), healthy subjects (controls) from patients with manifest diabetes mellitus of up to I year in duration who could not be distinguished through light microscopic or conventional morphometric studies.
Assuntos
Nefropatias Diabéticas/diagnóstico , Diagnóstico por Computador , Rim/anatomia & histologia , Adolescente , Adulto , Biópsia , Diabetes Mellitus Tipo 1/complicações , Nefropatias Diabéticas/patologia , Feminino , Humanos , Rim/patologia , Glomérulos Renais/anatomia & histologia , Glomérulos Renais/patologia , Masculino , Microcomputadores , Pessoa de Meia-IdadeRESUMO
Conformation of double-stranded complexes of polyriboguanylic acid with halogenated polyribocytidylic acid [poly(C)] was studied with the aid of differential pulse polarography, terbium fluorescence and circular dichroism spectrometry. It was shown that halogenation at C(5) of cytosine residues in poly(C) disturbed the ordered structure of the double-helical complex. In addition, this halogenation does not improve antiviral activity of the polynucleotide complex studied in the system of vesicular stomatitis virus and the cell culture of chicken embryos. It was concluded that the regularity of the secondary structure of synthetic RNAs might play an important role in the mechanism of biological activity of these biomacromolecules.
Assuntos
Bromo/química , Cloro/química , Poli C/química , Poli G/química , RNA de Cadeia Dupla/química , Fenômenos Biofísicos , Biofísica , Dicroísmo Circular , Polarização de Fluorescência , Conformação de Ácido NucleicoRESUMO
The modification of the double-stranded poly(G).poly(C) complex by cis-diamminedichloroplatinum(II) was studied by two modes: the action of cis-DDP on poly(G) before formation of the duplex with poly(C) and that on the prepared duplex. It was shown that in the latter case modification disordered the integrity of the duplex only negligibly at rb less than or equal to 0.05 and led to improved interferon-inducing and antiviral activity tested on mice infected by Influenza and Herpes viruses.
Assuntos
Cisplatino/química , Poli C/química , Poli G/química , Conformação de Ácido NucleicoRESUMO
The integrity of the double-stranded complex polyriboguanylic.polyribocytidylic acid [poly(rG).poly(rC)] modified by antitumour cis-diamminedichloroplatinum(II)(cis-DDP) was studied with the aid of differential pulse polarography and terbium fluorescence measurement. The modification was made to level corresponding to rb = 0.05 (rb is defined as the number of platinum atoms covalently bound per one nucleotide residue). Two modes of the modification of the polynucleotide complex were employed: The action of cis-DDP on poly(G) before formation of the complex with poly(C) and on the complex already formed from non-modified polynucleotides. It was shown that in the latter case modification disordered the integrity of the complex only negligibly. while in the former case the modification resulted in a noticeably more extensive disturbance of the double-stranded polynucleotide complex. Moreover, the modification of the complex (after its formation) at rb = 0.02 led to improved interferon-inducing and antiviral activity of poly(rG).poly(rC) tested on mice infected by influenza virus. It was suggested that the combined effects of interferon-inducing and antiviral activities of poly(rG).poly(rC) and antiviral activity of cis-DDP may result in an increased effect over and above what may be expected from the actions of the two modalities separately.
Assuntos
Cisplatino/farmacologia , Poli C/metabolismo , Poli G/metabolismo , Antivirais/metabolismo , Cromatografia em Gel/métodos , Fluorescência , Indutores de Interferon , Peso Molecular , Polarografia/métodos , Poli C/química , Poli G/química , Térbio/metabolismoRESUMO
The changes of hepatic mitochondrial cells were studied in white male rats during internal application of boron-containing waters, having 250 mg/l concentration of boron, but different ionic-salt basis. Mitochondrial hepatocytes and hepatic boron content relationships were established. It was shown that during boron accumulation in liver (15 applications) decreasing of mitochondrial size occurred, independently, of the type of boron-containing waters. During stable boron level and particularly during its excretion from organism (24 applications, 6 days aftereffect), increase of size of mitochondria and their ellipticity, change of configuration, that depend on the type of applied waters was shown.
Assuntos
Boro/efeitos adversos , Mitocôndrias Hepáticas/ultraestrutura , Abastecimento de Água , Animais , Boro/administração & dosagem , Boro/análise , Fígado/análise , Fígado/efeitos dos fármacos , Masculino , Mitocôndrias Hepáticas/efeitos dos fármacos , Modelos Biológicos , Ratos , EspectrofotometriaRESUMO
Specific reagents comprising digitonin bound to latex spheres were used as visual markers for the detection of cholesterol sites on the endothelial cell surface by scanning electron microscopy. The distribution of latex markers in the plasmolemma of the endothelial cells was investigated. These markers have strong bonds with the ligands. This interaction guarantees high specificity of binding between labeled markers and cellular membrane cholesterol.
Assuntos
Aorta/metabolismo , Membrana Celular/metabolismo , Digitonina/metabolismo , Animais , Sítios de Ligação , Colesterol/metabolismo , Endotélio/metabolismo , Indicadores e Reagentes , Látex , Microscopia Eletrônica de Varredura , CoelhosRESUMO
Modification of poly(C) by various frequency treatment with adenosine non-complementary to guanosine has produced poly(G) X poly (C.A) complexes with continuous double-stranded areas the length of which is determined by C/A ratio. Studies of the antiviral activity of poly(G).poly(C,A) complexes with C/A from 10:1 to 90:1 and poly(G).poly(C) in vesicular stomatitis virus-infected chick embryo cell cultures and in experimental tick-borne encephalitis of mice demonstrated that the maximum activity is achieved at an average lengths of double-stranded areas of 90 nucleotide pairs. At the same time, a low but statistically significant antiviral activity is observed at a length of double-stranded areas of 10-30 nucleotide pairs.
Assuntos
Antivirais , Poli C/farmacologia , Poli G/farmacologia , Polirribonucleotídeos/farmacologia , Animais , Composição de Bases , Embrião de Galinha , Avaliação Pré-Clínica de Medicamentos , Encefalite Transmitida por Carrapatos/tratamento farmacológico , Hidrólise , Camundongos , Camundongos Endogâmicos BALB C , Poli C/síntese química , Poli C/uso terapêutico , Poli G/síntese química , Poli G/uso terapêutico , Relação Estrutura-Atividade , Vírus da Estomatite Vesicular Indiana/efeitos dos fármacosRESUMO
By means of SEM-analysis in 110 microphotos (from 14 aortas of white rats) with the aim to estimate heteromorphism of the thoracic part endothelium, quantitative characteristics of endotheliocytes have been studied in the ventral and dorsal surfaces, and also around the ostia of the intercostal arteries. For the quantitative analysis organization of the lines of the interendotheliocyte borders is taken into account. Endotheliocytes around the ostium are more elongated but occupy less area and have less straight contours in comparison with cells in other regions; they have also greater variability. Essential differences in arranging the endothelial cells into the layer on the ventral and dorsal surfaces of the aorta are revealed.
Assuntos
Aorta Torácica/ultraestrutura , Endotélio Vascular/ultraestrutura , Animais , Contagem de Células , Técnicas Citológicas , Masculino , Microscopia Eletrônica de Varredura , Fotomicrografia , RatosRESUMO
A method of automated morphometric analysis of hepatic cell interphase nuclear chromatin (CHIN) structure was elaborated using scanning microspectrophotometer SMP-05 ("Opton", FRG). Liver sections of rats were used to investigate hepatic cell CHIN structure. The animals were administered ethanol intraperitoneally once per day within a month period in doses 4, 6, 8, 10 g/kg (1/3-4/5 LD50). Morphometric indexes indicating dose-dependent changes in hepatic cell CHIN structure are demonstrated. Statistical analysis of morphometric data has led to the conclusion that hepatic cell CHIN structural modification depends on two factors: ethanol damaging effect and hepatic cell regeneration.
Assuntos
Intoxicação Alcoólica/patologia , Cromatina/efeitos dos fármacos , Fígado/efeitos dos fármacos , Animais , Cromatina/ultraestrutura , Interfase , Fígado/patologia , Masculino , RatosRESUMO
It was established minimal length of continuous poly(C) sequence of poly(G).poly(C) complex required for effective interferon induction by investigation poly(G).poly(C,A) with different molar ratios of C:A varying from 10:1 to 90:1. The minimum length of the double-stranded sequence of the macromolecule complex poly(G).poly(C) is equal to at least 90-100 nucleotides. The effect of 5-halogen-polyribocytidilates on the properties of the complexes has been also investigated.
Assuntos
Poli C/síntese química , Poli G/síntese química , Polirribonucleotídeos/síntese química , Difosfato de Adenosina , Cistina Difosfato , Poli ARESUMO
Following are the results of using the elaborated algorhythms of the structural analysis for the study of the form elements of the blood. A possibility was demonstrated to carry out a differential diagnosis of the familial hyperlipoproteidemia of the second type according to the quantitative structural parametres of the chromatin of the inter-phase nuclei of the lymphocytes of the peripheral blood. A method was developed of the automated morphometry of erythrocytes making it possible to assess the optical profile of erythrocytes according to 16 parametres. It was also registered a more vivid change of the curve of the membrane of the erythrocytes as compared with the Price-Jones spread on the same erythrocytes. With a help of a system of proposed parametres, which are 4-6-fold more informative and sensitive, than the traditional ones, it is possible to obtain the qualitatively new characteristics of the forms of erythrocytes, and not only their sizes.