Behavioural plasticity of motor personality traits in the common vole under three-day continual observation in a test box.

In animals, behavioural personality traits have been well-documented in a wide array of species. However, these traits, different between individuals, are not completely stable in individuals. They show behavioural plasticity like many other phenotypic traits. This plasticity is able to overcome some weak aspects of personality trait behavioural strategy. In the present study, we examined the relationship between motor personality traits and behavioural plasticity in the common vole (Microtus arvalis) using a PhenoTyper (PT) box (Noldus). During a three-day test, four behavioural motor activity parameters were monitored in 47 voles: distance moved, (loco)motion duration, motion change frequency, sprint duration. Consistency repeatability (RC) of the parameters from the PT test was very high, with all values ≥ 0.91. To select the best linear mixed-effect models (LMMs), several predictors (test day, sex, body weight) were tested. Only test day had a significant effect on the dependent variables and other predictors did not improve the LMMs. Further, we found significant effects of random intercepts (motor personality traits) and slopes (behavioural plasticity), as well as significant negative correlations between them for all behavioural parameters. Our results indicate that motor personality traits were connected with behavioural plasticity. Moreover, we revealed a significant positive correlation between the random slopes of (loco)motion duration and motion change frequency. This relationship could indicate some central plasticity of motor personality traits. In conclusion, negative correlations between the motor personality traits and the behavioural plasticity demonstrate expression of convergent tendency from both opposite trait values. This corresponds with different ideas on ability to compensate personality effects or to prepare for potential future conditions. In the laboratory, plasticity of personality traits take place whenever an animal is placed e. g. in a breeding box for the first time or is left for a long time in an experimental apparatus.

Labelling of the anti-III-neurotubulin monoclonal antibody by 99mTc and its binding to responsible antigen.

BACKGROUND: beta-III-neurotubulin is a characteristic degradation protein of cellular cytoskeleton of nervous tissues, which originates with disorders that lead to the loss of peripheral neurons or neurons of the central nervous system. For the diagnostics of neuropathic and neurodegenerative processes, beta-III-neurotubulin is therefore the goal structure, and radioactive labelled antibody TU-20 (99m)Tc could theoretically enable diagnostic application in vivo. MATERIAL AND METHODS: In our case, were selected three ways of labelling monoclonal antibody IgG1-TU-20, which shows a high affinity towards beta-III-neurotubulin with (99m)Tc. The indirect labelling was ensured through the bifunctional chelator HYNIC, the direct labelling by electrolytic means, and the direct labelling of antibody TU-20 reduced by 2-mercaptoethanol. To observe each single feature, the appropriate chemical and biochemical control methods were used. Chemical purity was ensured by gel filtration and, together with chemical stability, it was checked by paper chromatography. To control the biological stability, SDS electrophoresis was used. The immunoreactivity was checked using ELISA-tests. RESULTS: The results have shown that the optimal method for labelling the antibody TU-20 is indirect labelling through the bifunctional chelator HYNIC, and the least effective method of labelling the antibody TU-20 is reduced by 2-mercaptoethanol. DISCUSSION: The results of labelling the monoclonal antibody towards antigen TU-20 with (99m)Tc confirmed that structure of the monoclonal antibody is destroyed by 2-mercaptoethanol, and in the case of electrolytic labelling, there are not enough binding places for radionuclide (99m)Tc on the monoclonal antibody.