RESUMO
Bacterial strain Vibrio sp. (PIGB 184) isolated from water samples of the Arabian Sea and identified through 16S rRNA demonstrated the production of pigmentary antioxidants with higher ABTS activities 90.9 ± 0.42 % in comparison with the standard commercial pigmented antioxidant, quercetin 88.8 ± 1.4 %. Antioxidative metabolites of this strain substantially inhibit the lipid peroxidation (LPO) reactions tested in sheep liver and brain. The antioxidant compounds produced by the Vibrio sp. (PIGB 184), analysed by GC-MS, reveals that it is composed mostly of phenol, 2,4-bis(1,1-dimethylethyl) and pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl). The interrelationship assessed between LPO and the phenolic compounds showed significant correlation with anti-LPO properties (R (2) = 0.9698 to 0.9861). These compounds are responsible for obstruction of harmful radical associated biochemical reactions in biological systems. Pigmented metabolites also tested for attributive biological properties against pathogenic bacteria showed prominent inhibition towards Gram-positive organisms (31.25 to 62.5 µg ml(-1)). From this study, it may be suggested that the marine bacterium PIGB 184 could be used as a potential bio-resource for antioxidants and needs to be worked out for mass production.
Assuntos
Antioxidantes/química , Fenóis/química , Pigmentos Biológicos/química , Pirróis/química , Animais , Antioxidantes/isolamento & purificação , Antioxidantes/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Lipídeos de Membrana/química , Lipídeos de Membrana/metabolismo , Fenóis/isolamento & purificação , Fenóis/metabolismo , Pigmentos Biológicos/biossíntese , Pigmentos Biológicos/isolamento & purificação , Pirróis/isolamento & purificação , Pirróis/metabolismo , RNA Ribossômico 16S/genética , Ovinos , Vibrio/química , Vibrio/genéticaRESUMO
A novel Gram-negative, motile, rod-shaped, facultative anaerobic bacterial strain, KMK6(T), was isolated from soil contaminated with textile dyes from an industrial estate located at Ichalkaranji, Maharashtra, India, and its taxonomical position was established by using a polyphasic approach. The major cellular fatty acids included C17:1ω8c, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), C17:0, C16:0, and C18:1ω7c. The DNA G+C content of strain KMK6(T) was 48.8 mol %. 16S rRNA gene sequence analysis confirmed its placement in the genus Alishewanella, and exhibited sequence similarity levels of below 97 % to the type strains of validly published Alishewanella species. On the basis of genotypic and phenotypic evidence, strains KMK6(T) is considered to be a novel species of the genus Alishewanella, for which we propose that strain KMK6(T) (=NCIM 5295(T) =BCRC 17848(T)) is assigned to a novel species, Alishewanella solinquinati sp. nov.
Assuntos
Alteromonadaceae/isolamento & purificação , Alteromonadaceae/metabolismo , Corantes/metabolismo , Microbiologia do Solo , Poluentes do Solo/metabolismo , Alteromonadaceae/classificação , Alteromonadaceae/genética , Biodegradação Ambiental , Corantes/análise , Dados de Sequência Molecular , Filogenia , Solo/química , Poluentes do Solo/análise , TêxteisRESUMO
Alishewanella sp. strain KMK6 was able to degrade mixture of textile dyes (0.5-2.0 g l(-1)) within 8 h. An initial 28 % reduction in COD was observed immediately after decolorization at static anoxic conditions which on further incubation at shaking conditions reduced by 90 %. Partially purified azoreductase was able to utilize different azo dyes as substrates. The HPLC profile of dye degradation showed formation of metabolic products. Further FTIR analysis showed significant changes in the peaks corresponding to functional groups present in dye mixture and its degradation products. The genotoxicity assessment showed that the dye degradation products were non-toxic compared to dye mixture.
Assuntos
Alteromonadaceae/metabolismo , Corantes , Têxteis , Carbono/metabolismo , Cromatografia Líquida de Alta Pressão , NADH NADPH Oxirredutases/metabolismo , Nitrogênio/metabolismo , Nitrorredutases , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
Alishewanella sp. strain KMK6 was isolated from textile dye-contaminated soil. The strain was able to decolorize and degrade different azo dyes and displayed high dye degradation ability and tolerance. The bacterium could completely degrade 2.5 g l(-1) dye, Reactive Blue 59 within 6 h. The induction in the level of cytochrome P-450 and activities of azoreductase and NADH-dichlorophenolindophenol reductase were observed in the cells after dye decolorization indicating the role of these enzymes. The intermediates of Reactive Blue 59 degradation were identified by high-performance liquid chromatography, gas chromatography and mass spectroscopy, and Fourier transform infrared spectroscopy. The ecotoxicity has been evaluated for dye and its metabolites by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (a yellow tetrazole) and comet assay, and it revealed that the dye degradation products were nontoxic.
Assuntos
Alteromonadaceae/metabolismo , Compostos Azo/metabolismo , Corantes/metabolismo , Alteromonadaceae/classificação , Alteromonadaceae/genética , Alteromonadaceae/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Sistema Enzimático do Citocromo P-450 , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Microbiologia Ambiental , Poluentes Ambientais/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Dados de Sequência Molecular , NAD/metabolismo , NADH NADPH Oxirredutases/metabolismo , Nitrorredutases , Quinona Redutases/metabolismo , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
The present study deals with development of aerobic granules from textile wastewater sludge and challenged with different concentration of reactive blue 59 (RB59) to test their dye degradation potential. The granules efficiently degraded reactive blue 59 and also sustained higher dye loading of up to 5.0 g l(-1). The significant induction of enzymes azoreductase and cytochrome P-450 indicated their prominent role in the dye degradation while genotoxicity studies demonstrated that the biotransformed product of the dye as non-toxic. The microbial community of the textile dyes degrading aerobic sludge granules analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), revealed significantly diverse dye degrading microbial community belonging to alpha-, beta-, and gamma-proteobacteria.
Assuntos
Compostos Azo/metabolismo , Bactérias Aeróbias/metabolismo , Corantes/metabolismo , Esgotos/microbiologia , Poluentes da Água/metabolismo , Purificação da Água/métodos , Compostos Azo/isolamento & purificação , Biodegradação Ambiental , Cor , Corantes/isolamento & purificação , Poluentes da Água/isolamento & purificaçãoRESUMO
The release of azo dyes into the environment is a concern due to coloration of natural waters and due to the toxicity, mutagenicity and carcinogenicity of the dyes and their biotransformation products. The dye degrading bacterial strain KMK 5 was isolated from the textile dyes contaminated soil of Ichalkaranji, Maharashtra, India. It was identified as Bacillus fusiformis based on the biochemical and morphological characterization as well as 16S rDNA sequencing. KMK 5 could tolerate and degrade azo dyes, Disperse Blue 79 (DB79) and Acid Orange 10 (AO10) under anoxic conditions. Complete mineralization of DB79 and AO10 at the concentration of 1.5g/l was observed within 48h. This degradation potential increased the applicability of this microorganism for the dye removal.