[Modified HPLC method of determination of the valproic acid in biological fluids].

Proposed modified HPLC method for determination of valproic acid in biological fluids. Created solid-phase extraction of valproic and heptanoic acids (internal standard, IS) on the cartridges packed hyper cross-linked polystyrene which maintain some tens extractions without losses of efficiency. Carboxylic acids are derivative with 1-(bromoacetyl)pyrene in acetone at presence of triethylamine. Chromatographic separation of derivatives is performed on Chromolith Perfonnance RP-18e columns, which packed unique monolithic sorbent. UV detection at 360 nm. Mobile phase acetonitrile - water (90:10, v/v) plus 1% isopropanol, speed flow 2000 microL/min, pressure 21 bar. Complete chromatographic cycle less than 3 minutes. Yield of IS and valproic acid (extraction plus derivatization) was 101-106%. Sensitivity (limit detection) was near 1 ng for valproic and near 0.6 ng for heptanoic acid during signal/noise ratio = 3.

[The HPLC analysis of vanillil-almond, 5-hydroxiindolacetic, homovanillic and homogentisic acids in biologic fluids in clinical laboratory].

The simple and fast HPLC technique of analysis of vanillil-almond, 5-hydroxiindolacetic, homovanillic and homogentisic acids in urine with solid-phase extraction using ultra cross-linked polystyrene (Purosep-200) is proposed. The separation on column Chromolith Performance RP-18e "Merck" 100x4.6 mm with monolithic phase-reversed silica gel in isocratic or gradient mode with ultraviolet detection under 285 nm. The isocratic mode is applied in case of ordinary analysis of vanillil-almond or homogentisic acids. The composition of eluent is isopropanol - water - TFA (1:99:0.025, v/v/v), flow speed is 1400 mkl/min; pressure is 37 bar, full separation less than in 4 min. The gradient low pressure mode is applied to analyze vanillil-almond, hydroxiindolacetic and homovanillic acids. Under this approach the switching to second eluent occurred from second minute. The composition of eluent is isopropanol-water-TFA (6:94:0.025, v/v/v), flow speed is 1400 mkl/min, pressure is 43 bar, full separation is less than in 7 min. The output (extraction share) consisted 78-113%. The simplicity reproducibility and sufficient sensitivity of technique in combination with the possibility of its application on standard chromatographic equipment (isocratic pump and ultraviolet detector) made it useful for routine clinical application.

[The scope of clinical applications of noninvasive methods for the assessment of liver fibrosis results of orginial studies in a multi-field hospital].

Potential and limitations of non-invasive methods for the evaluation of hepatic fibrosis are discussed (hepatic elastography, fibrotest, ultrasound dopplerography of portal vessels). Each non-invasive method was assessed in terms of threshold values it provides for the characteristic of successive stages ofhepatic fibrosis. The data obtained were used to develop the optimal algorithm for the diagnosis of hepatic fibrosis in patients with chronic viral liver infection.

[Correction by common sea buckthorn bark and sprout extracts of hormonal and metabolic disturbances during stress in rats]. / Korrektsiia ékstraktom kory i pobegov oblepikhi krushinovidnoi narushenii gormonal'no-metabolicheskogo statusa organizma krys v usloviiakh stressa.

Long-term administration of the common sea buckthorn bark and sprout extract improves the hormonal-metabolic organism status in rats disturbed by a stress factor (immobilization). The drug administration led to normalization of the altered functional activity indices of the neuro-endocrine system (disturbed adrenocorticotropin, 11-deoxycortisol, insulin, urea, and glucose levels) by affecting the production of glucocorticoids and increasing the hypothalamus sensitivity with respect to regulatory signals.

[Correction by natural adaptogens of hormonal-metabolic status disorders in rats during the development of adaptation syndrome using functional tests with dexamethasone and ACTH]. / Korrektsiia adaptogenami prirodnogo proiskhozhdeniia narushenii gormonal'no-metabolicheskogo statusa organizma krys v protsesse razvitiia adaptatsionnogo sindroma pri provedenii funktsional'nykh prob s deksametazonom i AKTG.

Rats exposed to stress by fixation develop a complex of hormonal metabolic homeostasis disturbances (as evidenced by changed levels of ACTH, insulin, 11-HOCS, urea, glucose). One of the major mechanisms of these disorders in reduced hypothalamus sensitivity to regulatory signals and exhausted adrenocortical functional activity, developing at the stages of anxiety and exhaustion of the adaptation syndrome, respectively, and detectable by functional tests with dexamethasone and ACTH. Administration of natural adaptogens (Scutellaria baicalensis extract and its active principle, baikalin flavonoid) was conducive to normalization of the majority of the examined parameters whatever the direction of changes.