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1.
Int J Legal Med ; 116(5): 286-8, 2002 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-12376839

RESUMO

In a case of the death of a 7-year-old boy, the police investigations revealed a possible dog attack contrary to the witness testimonies. DNA investigations were carried out from hairs, saliva and bloodstains with 10 canine-specific STR loci by the use of fluorescently labelled multiplex PCR and the ABI PRISM 310 genetic analyzer. The analysis of one hair sample revealed one allele deviation from the profile of the putative Rottweiler perpetrator possibly caused by a mutation. The PCR fragments in question at the PEZ20 locus were sequenced and compared with the alleles detected in the Hungarian canine population and identified on a repeat number basis. The allele frequencies were determined based on typing of 242 genetically independent canine individuals from 72 breeds. The results suggested that two of the canine individuals could be the perpetrators.


Assuntos
Cães/genética , Medicina Legal , Cabelo/química , Sequências de Repetição em Tandem , Animais , Mordeduras e Picadas , Manchas de Sangue , Criança , Mapeamento Cromossômico , Frequência do Gene , Humanos , Masculino , Mutação , Reação em Cadeia da Polimerase , Saliva/química , Especificidade da Espécie
2.
Acta Vet Hung ; 49(3): 269-73, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11702337

RESUMO

A case of disputed paternity in dogs is reported. DNA examinations were carried out from hair samples of the individuals several months after the death of the putative sire. Ten short tandem repeat (STR) loci were analysed by fluorescence-labelled multiplex PCR using ABI PRISM 310 Genetic Analyser. Based on the results the candidate sire was included in the pedigree records as the biological sire. In spite of the genetic homogeneity of pedigree dogs due to inbreeding, canine microsatellites can provide an adequate basis for assigning paternity in pure breeds.


Assuntos
Cães/genética , Repetições de Microssatélites/genética , Animais , Feminino , Cabelo/química , Masculino , Paternidade , Linhagem , Reação em Cadeia da Polimerase/veterinária
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