[The significance of individual inhalation allergens in the sensitization of children with respiratory allergy]. / Znacaj pojedinih inhalacionih alergena u senzibilizaciji dece sa respiratornim alergijskim oboljenjima.

Four hundred children were skin tested with following allergens: Dermatophagoides pteronyssinus, moulds, animal dander (mixture of dog, cat, sheep and horse dander), tree pollens, weed pollen (Ambrosia artemisifolia), and six grass pollens, as well as three nutritive allergens in children less than three years of age. Results demonstrated that in 200 children with perenial rhinitis and/or asthma. Dermatophagoides gave positive results in over 90%. In 200 children with pollen allergy, grasses were major allergens, and among them Timothy (Phleum pratense) gave over 90% of positive results. It is to be concluded than skin testing with many inhalatory allergens is not necessary in patients with typical clinical picture. Skin test with Dermatophagoides and Timothy should be done, and further evaluation taken into consideration only if these are negative.

[Chemotherapy and mutagenesis. A study of chromosome aberrations as a test for mutagenesis after the use of cyclophosphamide, methotrexate and cytosine arabinoside]. / Hemioterapija i mutageneza. Ispitivanje hromosomskih aberacija kao testa za mutagenezu posle primene cyclophosphamida, methotrexata i cytosine arabinosida.

Determination of chromosomal aberration in the lymphocyte cell culture of the peripheral blood in 50 children with malignant and nonmalignant diseases was the test applied in the investigation of the adverse effects of the cytostatic therapy on the human genome. The study included the cytostatic drugs cyclophosphamide, methotrexat and cytosine arabinoside which are used in the treatment for the autoimmune diseases, in organ and bone marrow transplantations and in malignant diseases. It was confirmed that these cytostatics could be the cause of considerable structural aberrations in chromosomes, particularly so when high dosages and long application are involved. Therefore it is understandable that these drugs as mutagens can be the cause of the secondary cancer in patients treated with cytostatic therapy and also of the congenital malformations in children by mothers treated with these medications.

[Chromosome abnormalities in malignant lymphoma]. / Hromosomske abnormalnosti u malignim limfomima.

Analysis of the karyotype from bone marrow tissue and lymphocyte culture of the peripheral blood was performed in 25 children either with Hodgkin's or non-Hodgkins' lymphoma prior to any treatment. numeric aberrations were confirmed in 40% of the patients; in these the hyperdiploid number of chromosomes ranged from 52 to 94, while 24% of the cases had cells with 47 chromosomes of which the surplus chromosome corresponded to those of the group C and, in one case, of the group G. The occurence of cells with abnormal chromosome sets confirmed presence of clonal evolution. This was also a poor prognostic sign. By using cytogenetic of high accuracy and by studying further the chromosome abnormalities, problems concerning the importance of these abnormalities in the etiology and epidemiology of these diseases might be solved.

[Cytogenetic examination of children with chronic granulocytic leukemia]. / Citogentska ispitivanja u dece sa hronicnom granulocitnom leukemijom.

The most important marker chromosome in patients with CDL is small acrocentric chromosome which is due to the deletion one of the chromosome 22. Ph 1 can be found only in adult type of CDL. Dhe authors present their experiences in citogeneticaly examined children with CDL. The examinations were done in six patients in peripheral blood and bone marrow. Ph 1 was negative only in one case. In the other patients philadelphia chromosome was found in bone marrow and in the peripheral blood only when the illness go from bad to worse.

[Congenital deficiency of humoral and cellular immunity]. / Urodeni nedostatak humorskog i celulnog imuniteta.

The study involves 10 children with primary immunologic deficiency of the humoral and cellular immunity diagnosed on the basis of the clinical symptoms of the disease and immunologic investigation. In 7 children, out of whome three were infants, suffering of recurrent respiratory tract infections, examinations revealed either absence or deficiency of the IgA in the serum and saliva. 2 children presented a classical picture of the Wiskott-Aldrich's syndrome followed by eczema, recurrent infections and trombocytopenia. Having studied the immunologic status in these two children in vitro and in vivo, the authors established deficiency in the humoral and cellular immunity. In an infant aged three months, with diagnosed histiocytosis X after histologic examination of the skin, the authors had examined the function of the T and B cells after which it was concluded that it was a case of rare form of the combined primary immunodeficiency.

[Blood groups in chromosome disorders]. / Izucavanje krvnih grupa u hromosomopatijama.

The authors investigated blood groups of two cases of polisomia, and 6 cases of chromosomal aberations. While the polisomia demonstrated no abnormalities of the blood groups, three cases of the chromosomal aberation show an alteration on the chromosome 2 and atypical inheritance of the MNSs groups, confirming autosomal location of these blood groups.

[Genetic counseling in hematologic diseases]. / Preporuke genetskog savetovalista u hematoloskim oboljenjima.

In patients with hereditary haematologic diseases risk of repetition of these anomalies in brothers and sisters of probands and their progenies was assessed. In haemiphilia and Wiskott-Aldrich's. thrombocytopenia, both caused by a mutant gene in an X chromosome, prenatal pole determination was consulted in order to have a pregnancy with a male fetus interrupted due to high risk. Dominantly hereditary spherocytosis, eliptocytosis, chronic benign neutropenia are the diseases which do not appear in such a severe form that ceasation of further pregnancies should be consulted, instead, an early postnatal diagnosis is suggested to the families with increased risk of these anomalies. In the recessive hereditary beta thalassemia and in Fanconi's anemia in which prenatal diagnosis can not be made for the time being, level of risk for the further children is told to the parents after the child has been born. The parents are then left to decide whether to take a risk and have other children who might suffer of these severe haemolitic diseases. In the haemolitic diseases with often occurrence in certain families, but in which the manner of inheritance is not clear, as for example in the Kasebach-Meritt's syndrome, risk for the following children is uncertainly increased so that the genetic counsel is uncertain and unprecise. In the hereditary haemolitic diseases which appear sporadically, either of known or unknown etiology, such as congenital teleangiectasis of capillary vessels or Sturge-Weber's syndrome with severe vascular and nerval disorders, the parents are stimulated to further pregnancies as they are not followed by increased risk.