RESUMO
To determine the most informative markers for assessing the functional state of endometrium during the 'window of implantation' and creating a model for assessment of the readiness of endometrium for embryo implantation. Forty-seven women with tubal infertility and a successful IVF pregnancy participated in the study. Pipelle endometrial sample was performed during the supposed 'window of implantation' in natural cycle with subsequent histological study, and transcriptional profile of genes GPX3, PAEP, DPP4, TAGLN, HABP2, IMPA2, AQP3, HLA-DOB, MSX1, POSTN determined by real-time quantitative polymerase chain reaction (qRT-PCR). Differences in the level of mRNA expression of all the studied genes in the receptive endometrium were found in comparison to the prereceptive one, which allowed us to classify two functional states of the endometrium. The results of histological examination responded to the stage of maturation of the endometrium in 78.7% of cases. Receptive endometrial status can be determined based on the integral evaluation of mRNA expression level of 4 PAEP, DPP4, MSX1, and HLA-DOB genes. The model for determining a personalized `window implantation' is offered for practical application in ART.
Assuntos
Implantação do Embrião/genética , Endométrio/metabolismo , Fertilização in vitro/métodos , Infertilidade Feminina/metabolismo , Adulto , Biomarcadores/metabolismo , Feminino , Expressão Gênica , Perfilação da Expressão Gênica , Humanos , Infertilidade Feminina/genética , GravidezRESUMO
The expression of mRNA of 36 genes involved in implantation was studied by reverse transcription and real-time PCR. Significant differences in mRNA expression during the early and middle stages of the secretion phase were detected for genes mmp7, vegf, il2m, il1ß, il8, il18, tnfα, il10, tgfß, igfbp2, etc.
Assuntos
Endométrio/metabolismo , RNA Mensageiro/genética , Adulto , Implantação do Embrião/genética , Feminino , Humanos , Interleucina-10/genética , Interleucina-1beta/genética , Metaloproteinase 7 da Matriz/genética , Reação em Cadeia da Polimerase em Tempo Real , Fator de Crescimento Transformador beta/genética , Fator de Necrose Tumoral alfa/genética , Fator A de Crescimento do Endotélio Vascular/genética , Adulto JovemRESUMO
Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic complication, the basis for which is a hyperergic uncontrolled ovarian response to gonadotropins in the ovulation stimulation cycles and assisted procreation programs. The clinical picture of OHSS is characterized by a broad spectrum of clinical and laboratory manifestations: increased vascular permeability resulting in the excess release of liquid into the third space and its storage with the development of hypovolemia, hemoconcentration, oliguria, hypoproteinemia, electrolyte imbalance, polyserositis; in severe cases, acute renal failure, thromboembolic events, and adult respiratory distress syndrome develop. The goal of treatment is to prevent the development of multiply organ dysfunctions. Its leading methods are infusion therapy with colloid-crystalloid solutions and anticoagulant therapy. Whether diuretics and other drugs are administered is under consideration. Indications for laparocentesis and peritoneal transudate evacuation are discussed. Complications requiring surgery are identified.
Assuntos
Cuidados Críticos/métodos , Insuficiência de Múltiplos Órgãos/prevenção & controle , Síndrome de Hiperestimulação Ovariana/terapia , Feminino , Humanos , Síndrome de Hiperestimulação Ovariana/sangue , Síndrome de Hiperestimulação Ovariana/epidemiologia , Síndrome de Hiperestimulação Ovariana/etiologiaRESUMO
The usage of 0.6-0.8 mg of lysenile in the course of 6 months gives a possibility to stimulate pregnancy in 70% of patients and gain pregnancy in 20% of patients. In order to exceed the percentage of impregnantion, the course of medicine can be enlarged up to 15 months.
Assuntos
Hiperprolactinemia/tratamento farmacológico , Infertilidade Feminina/tratamento farmacológico , Lisurida/uso terapêutico , Feminino , Humanos , GravidezRESUMO
The usage of metergoline in patients with infertility and different forms (hinds) of hyperprolactinaemia was studied for 90 days at a dose of 12 mg. It gives a possibility to stimulate ovulation in 64% of patients and gain pregnancy in 8% of patients. In order to exceed the percentage of inpregnantion the course of medicine can be enlarged up to 6 months.
Assuntos
Hiperprolactinemia/tratamento farmacológico , Infertilidade Feminina/tratamento farmacológico , Metergolina/uso terapêutico , Feminino , Humanos , Hiperprolactinemia/complicações , Infertilidade Feminina/etiologia , GravidezRESUMO
Clinical manifestations of various hyperprolactinemia-associated conditions were studied in infertile females. The results of hormone therapy were evaluated with regard to various preparations employed, pregnancy outcomes in those females were followed up.
Assuntos
Bromocriptina/uso terapêutico , Hiperprolactinemia/diagnóstico , Infertilidade Feminina/diagnóstico , Prolactina/metabolismo , Adenoma/diagnóstico , Adenoma/metabolismo , Feminino , Humanos , Hiperprolactinemia/complicações , Hiperprolactinemia/tratamento farmacológico , Infertilidade Feminina/tratamento farmacológico , Infertilidade Feminina/etiologia , Neoplasias Hipofisárias/diagnóstico , Neoplasias Hipofisárias/metabolismo , Prolactina/antagonistas & inibidores , Prolactina/sangue , Fatores de TempoRESUMO
28 women with hyperprolactinaemia were examined (prolactin levels varied from 720 to 66000mlU/l). These patients were subjected to the functional tests with GnRH, TRH and metoclopramid. In all groups of the patients, who were devided acording Prolactin levels, the sensitivity of lactotrophs was decreased. There is reserve correlation between levels of the prolactin and gonadotrophins (r = -0.64). The test with metoclopromid is more informative in the diagnosis of tumoral and functional hyperprolactinaemia.