[Indices of antioxidant system of blood in differential diagnosis of obliterating atherosclerosis and obliterating thromboangiitis of the lower limbs].

The work was aimed at elucidating the role of the indices of the antioxidant system (AOS) of the blood and nitrogen oxide content in differential diagnosis of atherosclerosis obiiterans (AO) and thromboangiitis obiiterans (TO) of the lower extremities. Presented herein are the findings of examining a total of one hundred and thirteen 30-to-45-year-old patients (of these, 60 had TO and 53 had AO) with various level of occlusion (from the femoropoplietal segment to the aortoiliac zone) and the stage of chronic arterial insufficiency (CAI IIA-IV). The control group was composed of 30 apparently healthy age-matched male subjects. Besides the clinical, laboratory and special instrumental methods of study, we determined the parameters of the AOS of blood and the content of nitrogen oxide degradation products. It was determined that the directedness and pronouncedness of deviations in the parameters of the AOS and nitrogen oxide from the physiological norm depended on not only the aetiology of the disease involved, but on the degree of tissue hypoxia predetermined by the stage of CAI of the lower limbs. For differential diagnosis between AO and TO, the most informative should be considered the coefficient: SOD activity/catalase activity, index of peroxide-luminol-dependent chemiluminescence and the content of nitrogen oxide degradation products.

[Effect of antitubercular preparations on enzyme activity of erythrocytes]. / Vliianie protivotuberkuleznykh preparatov na aktivnost' nekotorykh fermentov v éritrotsitakh.

It was established experimentally that antituberculous agents (isoniazide, rifampicin, methazid, ethambutol, PAS) and their combination (isoniazide + rifampicin + PAS; isoniazide + PAS; methazid + PAS) inhibit erythrocytic glucoso-6-phosphate-dehydrogenase, glycerophosphate-dehydrogenase. This should be considered in instituting individual antituberculous therapy.

[Activity of NAD- and NADP-containing enzymes and catalase in human erythrocytes after sucrose loading]. / Aktivnost' NAD- i NADP-soderzhashchikh fermentov i katalazy v éritrotsitakh cheloveka posle nagruzki sakharozoi.

The dynamics of glucose content and activity of GL-6-FDG, MDG, ICDG and of catalase in the erythrocytes of healthy people under glucose load was investigated. It has been established that maximal increase of the glucose content in blood under glucose load occurs 60 min later and the peak of activity of all the studied enzymes--90 min later. A degree of the activity increase in certain enzymes is not the same. It enhances considerably in GL-6-FDG and catalase and is hardly tracable in MDG and ICDG. A conclusion is made that glucose metabolism in erythrocytes is accompanied by the intensification of synthesis and hydrogen peroxide decomposition processes.

[Glucose utilization and activity of glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase and malate dehydrogenase in rat erythrocytes after treatment with tuberculostatic agents]. / Utilizatsiia gliukozy i aktivnost' gliukozo-6-fosfatdegidrogenazy, izotsitratdegidrogenazy i malatdegidrogenazy éritrotsitov krys pri deistvii tuberkulostaticheskimi preparatami.

Metazamide, phthivazid, larusan and rifamycin were shown to inhibit activities of glucose-6-phosphate-, isocitrate- and malate dehydrogenases in male rats within 10-14 days by 14-32%; as a result of this, the rate of glucose consumption was decreased in blood, as shown by the shape of sugar plots after loading with 300 mg of glucose. Administration of these drugs led to elevation of sugar curves by 11-18% as compared with controls. p-Amino-salicylic acid, isoniazid and protionamide inhibited the enzymatic activity but affected the rate of glucose consumption only slightly. Ethambutal activated these enzymes by 13-29%; the highest rate of glucose consumption was observed in presence of the drug.