RESUMO
The importance of nosocomial infections caused by coagulase-negative staphylococci is constantly growing. The threat primarily affects immunocompromised patients, the elderly and neonates, particularly after invasive surgery. The problem is fundamentally exacerbated by expanding antibacterial drug resistance. A case report is presented of an 86-year-old patient who underwent a ruptured abdominal aortic aneurysm surgery and developed septicaemia upon surgical wound infection. The causal agent was likely a carbapenem-resistant Klebsiella pneumoniae, however, daptomycin-resistant Staphylococcus pettenkoferi was identified in blood cultures in the absence of daptomycin treatment. To the authors' knowledge, the case study presented is the first published episode of daptomycin-resistant S. pettenkoferi strain.
Assuntos
Antibacterianos/farmacologia , Daptomicina/farmacologia , Choque Séptico/diagnóstico , Infecções Estafilocócicas/diagnóstico , Staphylococcus/efeitos dos fármacos , Idoso de 80 Anos ou mais , Sequência de Aminoácidos , Antibacterianos/uso terapêutico , Daptomicina/uso terapêutico , Farmacorresistência Bacteriana , Evolução Fatal , Genes Bacterianos , Humanos , Masculino , Testes de Sensibilidade Microbiana , Polimorfismo de Fragmento de Restrição , Choque Séptico/tratamento farmacológico , Choque Séptico/microbiologia , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/microbiologia , Staphylococcus/genética , Staphylococcus/isolamento & purificaçãoRESUMO
Genetic methods based on PCR-restriction fragment length polymorphism (RFLP) are widely used for microbial species determination. In this study, we present the application of saoC gene as an effective tool for species determination and within-species diversity analysis for Staphylococcus genus. The unique sequence diversity of saoC allows us to apply four restriction enzymes to obtain RFLP patterns, which appear highly distinctive even among closely related species as well as atypical isolates of environmental origin. Such patterns were successfully obtained for 26 species belonging to Staphylococcus genus. What is more, tracing polymorphisms detected by different restriction enzymes allowed for basic phylogeny analysis for Staphylococcus aureus, which is potentially applicable for other staphylococcal species.
Assuntos
Proteínas de Bactérias/genética , Variação Genética , Tipagem Molecular/métodos , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Staphylococcus/classificação , Staphylococcus/genética , Genótipo , HumanosRESUMO
Through a CAMP test, we first observed a Staphylococcus delphini strain (ATCC 49172) to release beta-haemolysin. Production of the latter in this coagulase-positive species of the 'Staphylococcus intermedius Group', in fact, has been labeled to be undetermined, thus far. Of course, a wider number of strains have to be investigated in order to define whether this property is constitutive (like in Staphylococcus (pseud)intermedius), or strain-dependent (like in Staphylococcus aureus), and which clinical impact it has; nevertheless, we can state that S. delphini ATCC 49172 indeed produces this toxin.