[The action of medichronal (glucose-glycine-formate) on the serotonin blood level in alcoholism patients]. / Deistvie medikhronala (gliukozoglitsinformiata) na soderzhanie serotonina v krovi bol'nykh alkogolizmom.

The effects of a novel antialcoholic medication medichronal on the serotonine concentration in blood of patients with second stage alcoholism was investigated. It has been discovered that remission in 75% of the patients was connected with its hyper- and hyposerotoninemic influence, depending on the initial lowered or increased serotonine content. Similarity of medichronal and unithiol effects was observed in the form of palyndromoinversion regulation of serotoninergic activity. In a portion of the patients the increased, compared to healthy persons, serotonine concentration before and after the treatment does not differ from the average values in the whole group.

[The new anti-alcohol preparation medikhronal: changes in the level of ethanol, acetaldehyde and monoamines during the treatment of alcoholism patients]. / Novyi antialkogol'nyi preparat medikhronal: izmenenie soderzhaniia étanola, atsetal'degida i monoaminov pri lechenii bol'nykh alkogolizmom.

Treating patients with stage-II alcoholism with medichronal and unithiol sharply decreases blood levels of alcohol and acetaldehyde. Medichronal restores the decreased level of norepinephrine excretion in 25% of patients and the increased level of epinephrine in 43%. The high baseline concentration of serotonin dropped in 41%, however, its level remained higher than in the controls. There was a negative correlation between the changes of ethanol concentrations and the excretion of norepinephrine. There was a direct dependence between the changes in the norepinephrine/epinephrine ratio and the serotonin concentration.

[The effect of an increased intensity of light on changes in the serotonin and melatonin content in patients with depression]. / Vliianie sveta povyshennoi intensivnosti na izmenenie soderzhaniia serotonina i melatonina u bol'nykh s depressiei.

Study into the effect of light of increased intensity shows that in patients with anxious depression a high level of melatonin excretion declines to normal, its low level in melancholic depression remaining unchanged. Blood concentrations of serotonin in depressive patients are higher as compared with the healthy persons and do not change with increasing in the brightness of the illumination except for a decrease in a portion of patients showing maximum levels of serotonin and melanin.

[The effect of protamine on cholesterol, triglycerides and blood serum proteins during the dynamics of experimental hypercholesterolemia]. / Deistvie protamina na kholesterin, triglitseridy i belki syvorotki krovi v dinamike éksperimental'noi giperkholesterinemii.

Content of total proteins, cholesterol, and triglycerides was studied in the blood serum and lipoprotein fractions of rabbits after administration of protamine within 1, 3 and 7 months of hypercholesterolemia development. The protamine effect was accompanied by "equalization" of cholesterol and triglyceride concentrations, as compared with their alteration in hypercholesterolemia, by stabilization of their content in various periods of the disease, but at the higher level than that of intact animals. The phenomenon observed occurred mainly in lipid components of LDL and VLDL subfractions. Analysis of alterations in the content of cholesterol and proteins in HDL and apo B-containing fractions showed that administration of protamine during long-term hypercholesterolemia led to development of antiatherogenic symptoms. Proatherogenic alterations in lipoprotein composition, developed during hypercholesterolemia, appear to be inhibited by protamine.

[Blood lipoproteins in modeling of atherosclerosis and administration of protamine]. / Lipoproteiny krovi pri modelirovanii ateroskleroza i vvedenii protamina.

The influence of protamine on the HDL, LDL, VLDL species concentration variation as well as on their cholesterol and protein content in respect to total blood serum cholesterol in different periods (1 and 7 months) of hypercholesteremia in rabbits is investigated. Daily administration of protamine (10 mg/kg) during 30 days to intact animals did not change the investigated parameters. In the early period of hypercholesteremia protamine considerably increased the content of all lipoprotein fractions. Under the conditions of prolonged hypercholesteremia the LDL and VLDL concentrations decreased by 23 and 60 per cent, respectively; HDL increased by 101 per cent.

[Effects of protamine on blood lipoproteins in the early period of hypercholesterolemia in rabbits]. / Deistvie protamina na lipoproteiny krovi v rannii period giperkholesterinemii u krolikov.

The content of protein, cholesterol and triglycerides in chylomicrons and different classes of blood serum lipoproteins was studied under protamine action in early (1 month) hypercholesterolemia. Except the low density lipoproteins the amount of triglycerides in the particles studied was practically unchanged. The content of proteins in all classes of lipoproteins was greatly increased and this may indicate that protamine activates the blood lipoprotein system. The enrichment of lipoproteins with cholesterol may be taken as being a result of protamine action in early hypercholesterolemia. The peculiar feature in the effect of protamine on mentioned lipoprotein components was that the protein/cholesterol ratio didn't change in chylomicrons, decreased in very low density lipoproteins and low density lipoproteins and increased in high density lipoproteins as compared with controls.

[Serum proteins in ethanol intoxication and hypercholesteremia]. / Belki syvorotki krovi pri intoksikatsii étanolom i kholesterinoze.

Guinea pigs after 30, 60 and 90 days of cholesterol, ethanol and cholesterol + ethanol action have been studied for content of cholesterol, lipoproteins of certain classes, quantitative and qualitative composition of blood serum proteins. It has been found that cholesterol does not induce expressed hypercholesterinemia and does not hinder cholesterol accumulation in the blood serum and liver of animals. The specific activity of [3H] cholesterol in the liver under cholesterinosis and its combination with ethanol intoxication for the whole period of experiments is lower than in the control, which testifies to retardation of its renewal. This may stimulate development of pathological hypercholesterinemia-induced states. After 3-month ethanol intoxication the amount of alkaline serum proteins has grown and ethanol retains its action against a background of hypercholesterinemia. The found effect is supposed to reflect one of the compensatory mechanisms for hypercholesterinemia and atherogenesis prevention.

[Changes in the serum protein composition in experimental hypercholesterolemia]. / Izmenenie belkovogo sostava syvorotki krovi pri éksperimental'noi giperkholesterinemii.

Cholesterol accumulation, quantitative changes and composition of lipoproteins, total proteins of blood serum and protein fractions obtained by acid extraction are studied in hypercholesterinemia dynamics in rabbits. It is found that the initial period of cholesterinosis in blood serum is marked by an increased content of total proteins and proteins extracted by acid followed, however, by substantial lowering of the level of these compounds. The proteins obtained by acid extraction are characterized by more explicit changes. This permits assuming their important role in the pathogenesis of hypercholesterinemia. The obtained results make it possible to state that the investigated proteins possess the alkaline properties. The data available in literature on the ability of alkaline polypeptides to bind cholesterol permit assuming that the investigated proteins have the same properties and, hence, can participate in the molecular mechanisms of cholesterol transport as well as in the processes of synthesis and transformations of separate classes of lipoproteins.

[Serum protein fractions in patients with dyscirculatory encephalopathy in atherosclerosis and hypertension]. / Belkovye fraktsii syvorotki krovi u bol'nykh s distsirkuliatornoi éntsefalopatiei pri ateroskleroze i gipertonicheskoi bolezni.

Using a novel method of electrophoresis, patients with atherosclerotic and hypertonic dyscirculatory encephalopathy were examined for protein fractions of their blood serum. Various kinds of dysproteinemia and dyslipoproteinemia were recorded, with their manifestations being particularly pronounced in cerebral atherosclerosis with the syndrome of arterial hypertension. Clinical improvement following the therapy was usually accompanied by the normalization of the protein spectrum of blood serum. The results obtained may be utilized in the differential diagnosis and the evaluation of the therapy efficacy.

[Use of disc electrophoresis in a linear gradient of polyacrylamide gel concentrations for simultaneous fractionation of total serum proteins, haptoglobins and lipoproteins]. / Primenenie disk-elektroforeza v lineinom gradiente kontsentratsii poliakrilamidnogo gelia dlia odnovremennogo fraktsionirovaniia summarnykh belkov syvorotki krovi, gaptoglobinov i lipoproteidov.

Conditions are developed for fractionation of total proteins of blood serum and lipoproteids within a linear gradient of the polyacrylamide gel concentration. Optimal separation of the proteins was observed at initial concentrations of acrylamide 3 and 10%: lipoproteids--2 and 15%. The composition of haptoglobins should be taken into consideration when analyzing the spectrum of human serum proteins. For this purpose samples fractionated simultaneously in one gel plate should be subjected to differential staining with amide black and benzidine. It is established that the efficiency of alpha-lipoproteids subfractions separation is reached by electrophoresis of this class lipoproteids after polyethylene glycol precipitation. The method described broadens essentially potentialities of electrophoretic fractionation of blood serum proteins due to an increase in the resolving power and sensitivity.