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Vet Microbiol ; 110(1-2): 27-33, 2005 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-16125879

RESUMO

Koi herpesvirus (KHV), which is believed to be an emerging virus, causes fatal diseases in carps. Since the 1990s, the presence of KHV has been confirmed in several countries. In Japan, from 2003 to 2004, large outbreaks of KHV infection in farmed carps resulted in the death of numerous fishes. From April to May 2004, we collected 43 dead or dying carps exhibiting typical symptoms of KHV infection in Gunma prefecture. To conduct a molecular epidemiologic study of KHV in our prefecture, we amplified DNA polymerase and the major envelope protein genes of KHV derived from carp gills using newly designed primers. We also performed sequence analysis of both genes of KHV. Sensitivity of our PCR method for amplification of DNA polymerase and the major envelope protein genes of KHV was 3 x 10(2) (100 fg) and 3 x 10(3) (1000 fg) copies of KHV genome, respectively. We detected both DNA polymerase and major envelope protein genes in 37 of 43 carps (86%). No mutation was found in both the genes sequenced from 11 strains, which included two foreign strains and one domestic strain. The results suggested that KHV strains derived from carps in our prefecture were closely related genetically to the other KHV strains.


Assuntos
Carpas/virologia , DNA Polimerase Dirigida por DNA/genética , Doenças dos Peixes/virologia , Infecções por Herpesviridae/veterinária , Herpesviridae/isolamento & purificação , Proteínas do Envelope Viral/genética , Animais , Sequência de Bases , DNA Viral/análise , Surtos de Doenças/veterinária , Doenças dos Peixes/diagnóstico , Doenças dos Peixes/epidemiologia , Amplificação de Genes , Brânquias/virologia , Herpesviridae/química , Herpesviridae/genética , Infecções por Herpesviridae/diagnóstico , Infecções por Herpesviridae/epidemiologia , Infecções por Herpesviridae/virologia , Japão/epidemiologia , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/veterinária , Sensibilidade e Especificidade
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