Biostimulant enhances growth and corm production of saffron (Crocus sativus L.) in non-traditional areas of North western Himalayas.

The usage of seaweed extracts in cropping systems is gaining attention nowadays due to their distinct bioactive properties. This study aims to assess how saffron (Crocus sativus L.) corm production was affected by seaweed extract through different application modes. The study was conducted at the CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP, India, during the autumn-winter agricultural cycle. Five treatments using a combination of Kappaphycus and Sargassum seaweed extracts were replicated five times in a randomized block design. Treatments that were examined include T1: Control, T2: Corm dipping @ 5% seaweed extract, T3: Foliar spray @ 5% seaweed extract, T4: Drenching @ 5% seaweed extract, and T5: Corm dipping + foliar spray @ 5% seaweed extract. Seaweed extract, when applied to saffron plants (T5: Corm dipping + foliar spray @ 5% seaweed extract) resulted in significantly higher growth parameters along with the higher dry weight of stem, leaves, corms, and total roots per corm. Corm production, viz., the number of daughter corms and corm weight per m2 was significantly affected by seaweed extract application, with the maximum value recorded with treatment T5. Biochemical parameters chlorophyll, carotenoids, and photosynthetic rate were higher in T5, while nutrient concentration was lowest in this treatment. Seaweed extracts improved corm production, making it a feasible alternative to limiting the application of conventional fertilizers, attenuating the effects on the environment, and enhancing corm number and weight.

Agro-Climatic Suitability Evaluation for Saffron Production in Areas of Western Himalaya.

Saffron (Crocus sativus L.) is an expensive spice crop cultivated successfully in Iran, Afghanistan, India, Greece, Morocco, Spain, and Italy. The present study was conducted during the periods 2018-2019 and 2019-2020 to evaluate the morphological, yield and quality parameters of saffron in six different regions of non-traditional areas of the western Himalayas. The two experimental factors were "year" and "location." The experiment was conducted using a factorial randomized block design with three replications. Yield attributes, viz., number of flowers, fresh flower yield, fresh stigma yield and dry stigma yield were significantly higher in location L3 compared to other geographical locations. Dry stigma yield in location L3 was higher by 50.0, 41.2, 33.3, 14.3, and 9.1% compared to locations L6, L5, L1, L4, and L2, respectively. These were characterized by the appropriate climatic conditions, viz., high altitude, sandy-loam texture of the soil, optimum temperature, lesser relative humidity and total rainfall, demonstrating that it is possible to cultivate this spice even in non-traditional areas of the western Himalaya. Positive correlations were established for stigma yield with increased altitude and lesser rainfall. Secondary metabolites viz., crocin and picrocrocin increased significantly with the increase in altitude; however, a reverse trend was recorded for safranal content. Total phenolics and flavonoids were significantly higher in the geographical location of Kinnaur, H.P. (L1) and Bharmour, H.P. (L4). In conclusion, the assessment of different geographical locations and soil types is particularly necessary to encourage saffron production and its qualitative traits. Based on current findings, saffron can be grown successfully in some non-traditional locations of the western Himalayan regions.

Menstrual Cycle Abnormalities in Patients with Prolactinoma and Drug-induced Hyperprolactinemia.

BACKGROUND AND OBJECTIVE: Hyperprolactinemia affects the reproductive endocrine axis; however, the degree of dysfunction may vary depending on etiology. The aim of the present study was to analyze menstrual cyclicity in patients with prolactinoma and drug-induced hyperprolactinemia (DIH). METHODOLOGY: Patients with prolactinoma and DIH were retrospectively analyzed for menstrual cyclicity at presentation and response to therapy. RESULTS: Of 128 females with hyperprolactinemia, 58 had prolactinoma (41 microadenoma and 17 macroadenoma) and 39 had DIH. Patients with prolactinoma had higher prolactin levels and increased frequency of oligomenorrhea (77.5% vs. 46%) as compared to DIH. Patients with macroprolactinoma had more severe menstrual disturbances compared to microprolactinoma. A higher percentage of patients with microprolactinoma and DIH achieved regular menstrual cycles compared to macroprolactinoma postcabergoline treatment (85% and 90% vs. 65%). There was no correlation between time to regularization of menstrual cycles with age, menstrual cycle length, duration of menstrual irregularity, or initial prolactin level in patients with prolactinoma. Linear regression analysis showed a significant association between time to regularization of menstrual cycles with time to normalization of prolactin levels (P = 0.001). CONCLUSION: There is a prompt restoration of menstrual cycles in patients with microprolactinoma and DIH. Patients with macroprolactinoma have more severe menstrual disturbances and lesser frequency of cycle restoration postcabergoline treatment compared to microprolactinoma and DIH.

Persistence of histoplasma in adrenals 7 years after antifungal therapy.

Adrenal histoplasmosis is an uncommon cause for adrenal insufficiency. The duration of treatment for adrenal histoplasmosis is not clear. Existing treatment regimens advocate antifungals given for periods ranging from 6 months to 2 years. We report here a rare case who showed persistence of histoplasma in adrenal biopsy 7 years after being initially treated with itraconazole for 9 months. This calls for a prolonged therapy with regular review of adrenal morphology and histology in these patients.

Osteomas of the craniofacial region: A case series and review of literature.

OBJECTIVE: To discuss the clinical presentation, diagnosis and management of osteomas involving the craniomaxillofacial region. MATERIALS AND METHODS: This study was conducted from June 2004 to March 2012 at our institute. A total of 12 cases between the ages of 10 and 50 years were managed with surgical excision and reconstruction. The criteria used to diagnose osteoma included radiographic and clinical features and histological confirmation of the specimen. The total follow-up period ranged from 6 to 24 months. RESULTS: Out of 12 osteomas, 10 were peripheral and 2 were centrally located. Mandible involvement was seen in six patients, four involved the orbit, one the frontal bone and one the frontal bone with the skull base. All patients undergoing excision and reconstruction had a favourable aesthetic and functional outcome. There were no recurrences and no post-operative complications. CONCLUSION: Osteomas affect all age groups with no sex predilection and are usually clinically asymptomatic till they become large in size. Surgical excision and appropriate reconstruction is the mainstay of management. Surgery is indicated when lesion is symptomatic or actively growing and the surgical approach for exposure of the lesion should be case specific.

The use of the temporoparietal fascia flap in various clinical scenarios: A review of 71 cases.

OBJECTIVE: This report details our experience with the use of the temporoparietal fascia flap in different scenarios of reconstruction and to discuss our technique of harvest, clinical applications, and review of literature of this versatile flap. MATERIALS AND METHODS: A retrospective study of 82 cases of temporoparietal fascia flap in 71 patients, operated over a period of 10 years was conducted. Patients were grouped based on various clinical indications. The follow up period ranged from a minimum of 1 to a maximum of 10 years (Mean-four and a half years). All patients were analyzed for functional and aesthetic outcome using preoperative and postoperative photographs. RESULTS: No significant complications were seen in our series. Only 2 out of 82 flaps had partial necrosis of flap (2.44%). Two patients who were operated for release of submucous fibrosis developed recurrence due to continued use of tobacco. The final outcome in one patient of ear reconstruction was unsatisfactory due to flap failure. The remaining patients had satisfactory functional and aesthetic outcomes (95.77%). None had other complications like temporal branch of facial nerve injury or alopecia along the scar line. CONCLUSION: The TPFF is one of the most reliable and versatile flap in the head and neck region. It can also be reliably used as free fascial flap. When its advantages are combined with the surgeon's resourcefulness, various defects can be successfully reconstructed using the temporoparietal facia flap with satisfactory aesthetic and functional outcome.

Spontaneous reduction of prolactinoma post cabergoline withdrawal.

Prolactinomas are common pituitary tumors usually highly responsive to dopamine agonists. Around 70-90% of the prolactinomas exhibit decrease in tumor size, though variably with these agents. Uncommonly, there may be little or no shrinkage in pituitary tumor. In the absence of medical therapy, pituitary apoplexy may also result in tumor shrinkage, albeit rarely. We report here a case showing only modest reduction in prolactinoma with cabergoline given for a period of one and a half years. Surprisingly, this tumor showed a 40% reduction in the tumor size 3 months after cabergoline withdrawal in the absence of clinical or radiological evidence of apoplexy.