Effect of guinea fowl egg storage duration on embryonic and physiological parameters, and keet juvenile growth.

This study investigated the effects of guinea fowl hatching eggs storage time on embryo parameters and post-hatch juvenile growth. A total 1,800 eggs of guinea fowl were used. They were numbered, weighed, and divided into four groups of 450 eggs each according to storage time of 3, 7, 11, and 15 D before storage at a temperature of 18°C. Then, they were incubated at 37.7°C and 55% relative humidity for 28 D in a forced-draft incubator. Egg weight loss, albumen pH and weight, embryo weight, hatching events, and keet growth up to 7 D post-hatch were recorded. In addition, thyroid hormone and corticosterone levels were determined. The results indicate that during storage, relative egg weight loss increased with storage duration. However, albumen pH increased with storage time up to 11 D of storage and remained unchanged between 11 and 15 D. In addition, from 19 to 22 D of incubation, albumen weight was higher for eggs stored for 15 D compared to that of eggs stored for 3 to 11 D. But, from 16 D of incubation, embryos from eggs stored for 3 D grew faster than those from eggs stored for 7 to 15 D. Incubation durations up to internal pipping (IP), external pipping (EP), and hatching events increased with egg storage duration. At IP, corticosterone and triiodothyronine (T3) concentrations of eggs stored for 15 D had the lowest (P < 0.05) compared to those of eggs stored for 3 to 11 D. Moreover, the levels of thyroxine (T4) decreased with storage duration (P < 0.05). At hatch, corticosterone levels increased while T4 levels decreased with storage duration (P < 0.05). Also, hatchability decreased with egg storage duration. In addition, 7-day-old keets from eggs stored for 3 and 7 D had comparable weight and were heavier than those from eggs stored for 11 D. It was concluded that storage of guinea fowl hatching eggs more than 7 D negatively affects egg quality and subsequently depresses embryo and post-hatch growth.

A Case of Rare Complication of Inguinal Parietoplasty according to Lichtenstein: Entero Cutaneous Fistula.

Lichtenstein intervention is currently the classic model of the regulated treatment of inguinal hernias by direct local approach. This "tension-free" technique satisfies both patients and practitioners. However, it does not often evade severe complications of parietal surgery. The authors report their treatment experience in rural Africa of a late enterocutaneous fistula which aggravated an inguinal hernia repair according to the Lichtenstein procedure. Physiopathology, diagnosis, and treatment of that disease are analyzed in the light of literature.

Next generation sequencing elucidates cacao badnavirus diversity and reveals the existence of more than ten viral species.

Cacao swollen shoot virus is a member of the family Caulimoviridae, genus Badnavirus and is naturally transmitted to Theobroma cacao (L.) by several mealybug species. CSSV populations in West African countries are highly variable and genetically structured into several different groups based on the diversity in the first part of ORF3 which encodes the movement protein. To unravel the extent of isolate diversity and address the problems of low titer and mixed viral sequences in samples, we used Illumina MiSeq and HiSeq technology. We were able to reconstruct de novo 20 new complete genomes from cacao samples collected in the Cocoa Research Institute of Ghana (CRIG) Museum and from the field samples collected in Côte d'Ivoire or Ghana. Based on the 20% threshold of nucleotide divergence in the reverse transcriptase/ribonuclease H (RT/RNase H) region which denotes species demarcation, we conclude there exist seven new species associated with the cacao swollen shoot disease. These new species along with the three already described leads to ten, the total number of the complex of viral species associated with the disease. A sample from Sri Lanka exhibiting similar leaf symptomology to West African CSSD-affected plants was also included in the study and the corresponding sequence represents the genome of a new virus named cacao bacilliform SriLanka virus (CBSLV).

Abcès du muscle psoas révélant une infection à VIH

Les auteurs rapportent l'observation d'un volumineux abcès primitif du muscle psoas révélateur d'une infection à VIH chez un adulte sans antécédent ni co-morbidité connus. L'abcès primitif du psoas est une affection rare. Son association avec l'infection à VIH apparait singulière. Nous exposons les particularités étiopathogéniques de cette association pathologique et les possibilités diagnostiques au regard de la littérature. La lombotomie reste notre traitement de choix des volumineux abcès du muscle ps

African and classical swine fever situation in Ivory-Coast and neighboring countries, 2008-2013.

This study was conducted from 2008 to 2013 to determine the animal health status of Ivory Coast and neighboring countries (Burkina Faso, Ghana, Togo and Benin) for African swine fever (ASF) and classical swine fever (CSF), and to assess the risk factors for ASF introduction in Ivory Coast. Ivory Coast had probably been free from ASF from 1998 to 2014 when it was re-introduced in this country. However, the ASF virus was found in all neighboring countries. In contrast, no evidence of CSF infection was found so far in Ivory Coast and neighboring countries. To assess the risk of ASF reintroduction in Ivory Coast, we surveyed 59 modern pig farms, and 169 pig owners in 19 villages and in two towns. For the village livestock, the major risk factor was the high frequency of pig exchanges with Burkinabe villages. In the commercial sector, many inadequate management practices were observed with respect to ASF. Their identification should enable farmers and other stakeholders to implement a training and prevention program to reduce the introduction risk of ASF in their farms.

[Relationship between human immunodeficiency virus (HIV-AIDS) and conjunctival squamous cell carcinoma: A clinical epidemiological study of 26 cases in the ophthalmology department of the university hospital of Treichville-Abidjan (Abidjan-Côte d'Ivoire)]. / Relation entre le virus de l'immunodéficience acquis (VIH-sida) et Carcinome épidermoïde conjonctival (CEC) : étude épidémioclinique de 26 dossiers au service d'ophtalmologie du CHU de Treichville (Abidjan-Cote d'Ivoire).

PURPOSE: To describe the epidemiological and clinical parameters of conjunctival squamous cell carcinomas and their association with HIV-AIDS in the ophthalmology department of the university hospital of Treichville. PATIENTS ET METHODS: This is a prospective and descriptive cross sectional study carried out in the ophthalmology department of the university hospital of Treichville from July 2007 to June 2011. Twenty-six (26) patients who were selected presented with a tumor of the globe and/or adnexa. A CT scan was performed in advanced cases. Histopathological examination of the surgical specimen was performed in all cases. HIV serology was performed after informed consent. All patients were referred to the oncology department of the university hospital of Treichville where a unit of care for HIV-positive patients with malignant tumors is available. Statistical analysis was performed using the software program EPI info version 6.0. RESULTS: We note a slight predominance of female patients 15 (57.69%) vs. 11 (42.3%) male patients. The mean age was 42.32 years, and 13 patients (50%) were between 25 and 45 years of age. Intraorbital tumors were the most common reason for consultation, i.e. 38.46%. Seventeen patients (65.38%) had no particular history. Nine patients (34.61%) were already on antiretroviral treatment on admission. Three patients, i.e. 11.54%, had already had tumor biopsy and/or evisceration. The mean duration of the disease before consultation in ophthalmology was 14.28 months. Localized limbal conjunctival tumors were found in 11 cases (42.38%). Advanced tumors with regional or cerebral extension accounted for 34.61% of cases. Six patients (23.078%) were HIV negative vs. 20 (76.92%) HIV positive patients. CD4 lymphocyte typing was performed in 17 patients (85%), and 45% had a CD4 count below 200. Only five patients were able to receive neoadjuvant chemotherapy, but three of them died. The average length of follow-up was 29 months. COMMENT: This study allowed us to establish a statistically significant link between HIV positive status of patients and the occurrence of squamous cell carcinoma, (P=10(-4)), as well as the decline in CD4 count and disease progression (p=10(-3)). CONCLUSION: The poor prognosis of conjunctival carcinoma reflects the low socioeconomic status of patients, physician under-staffing in our health care facilities, the prohibitive cost of anticancer drugs and the link between this disease and HIV-AIDS.

Severe antenatal strangulation and sudden fetal death occurs in term: case report.

The authors report a case of a sudden antenatal death, by severe strangulation, unlikely related in a term pregnancy; multiple loops of nuchal umbilical cord (UC) (ten), rarely describe in literature, were observed around the fetal neck. The in utero fetal death (IFD) was suspected by the non-attendance of fetal movements and confirmed by US scan. The tight nuchal cord around the neck (tCAN) diagnostic was made during caesarean delivery, as it was not discovered in pregnancy US scan monitoring nor in the US scan made in emergency. The newborn examination shows severe fetal strangulation by the presence of many spires of a too long UC (1.50 m). Autopsy was not been accepted by the family. Through this reported case the authors wanted to show the difficulties of its diagnosis in less developed Sub-Saharan country were US scan practice is not usual.

African Swine Fever Diagnosis Adapted to Tropical Conditions by the Use of Dried-blood Filter Papers.

The performance of Whatman 3-MM filter papers for the collection, drying, shipment and long-term storage of blood at ambient temperature, and for the detection of African swine fever virus and antibodies was assessed. Conventional and real-time PCR, viral isolation and antibody detection by ELISA were performed on paired samples (blood/tissue versus dried-blood 3-MM filter papers) collected from experimentally infected pigs and from farm pigs in Madagascar and Côte d'Ivoire. 3-MM filter papers were used directly in the conventional and real-time PCR without previous extraction of nucleic acids. Tests that performed better with 3-MM filter papers were in descending order: virus isolation, real-time UPL PCR and conventional PCR. The analytical sensitivity of real-time UPL PCR on filter papers was similar to conventional testing (virus isolation or conventional PCR) on organs or blood. In addition, blood-dried filter papers were tested in ELISA for antibody detection and the observed sensitivity was very close to conventional detection on serum samples and gave comparable results. Filter papers were stored up to 9 months at 20-25°C and for 2 months at 37°C without significant loss of sensitivity for virus genome detection. All tests on 3-MM filter papers had 100% specificity compared to the gold standards. Whatman 3-MM filter papers have the advantage of being cheap and of preserving virus viability for future virus isolation and characterization. In this study, Whatman 3-MM filter papers proved to be a suitable support for the collection, storage and use of blood in remote areas of tropical countries without the need for a cold chain and thus provide new possibilities for antibody testing and virus isolation.

Staphylococcal Scalded Skin Syndrome in Neonate.

We described a case of Staphylococcal Scalded Skin Syndrome in infant age of 21 days by discussing clinical and management issues. This newborn presented large erythematous, eroded, and oozing areas covered by epidermal skin flap. The average surface of cutaneous unsticking on admission was 31.35% of body surface area corresponding to lesions of superficial second-degree burns. An important biological inflammatory syndrome including positive C-reactive protein was found. Under treatment, erythroderma decreased within 7 to 10 days and the newborn was completely healed after 3 weeks of followup, with the disappearance of the inflammatory syndrome and total body surface restored. This clinical case report showed that SSSS remains a major dermatological problem in neonates. Therefore, its diagnosis should be made without doubt and its care should start earlier in a neonate emergency unit in order to have good prognosis. And the rigorous "search and destroy" policy based on screening of staff and patients and isolation of identified patients advocated in the United Kingdom should be applied in neonate units in Côte d'Ivoire.

[Post-partum sexuality. Living in black African couple analysis]. / La sexualité du post-partum. Analyse du vécu dans le couple noir africain.

INTRODUCTION: The period of the post-partum arouses a lot of questioning in the couple in particular regarding sexuality. OBJECTIVE: Analyze the real-life experience of the sexuality of the African black couples in the post-partum. PATIENTS AND METHOD: It was about study type attitudes and practical knowledge which took place in the department of gynecology-obstetrics and andrology of the teaching hospital of Bouaké over the period going in September 30th, 2012 on April 30th, 2013. The study took place in two stages. The first stage had consisted in a pre-test which allowed validate the questionnaire which contained 16 items. The second stage consisted submitting it directly to the patients, in postnatal consultation, who had agreed to participate in the study after an informed consent. The data were analyzed with epi-info, 2002 version. RESULTS: On 395 patients investigated, we noted the resumption of the sexual intercourse for 140 patients (34,2 %). The resumption was made on the initiative of the partner in 67,9 % of the cases, in particular after the return of menstruation (53,8 %). The reason to accept sexual intercourse was to satisfy the partner (60,7 %) and to maintain the harmony of the couple (57,1 %). However, the weekly frequency of the sexual intercourse had decreased in 75 % of the couples. This decrease was caused by the time dedicated to the child (66,7 %) and by the dyspareunia (57,1 %). We also observed a more important frequency of the anal sexual intercourse in the post-partum compared with the period before the childbirth (17,8 % vs. 3,57 %). Also, the use of subtleties, such as lubricants was observed more frequently in the post-partum (39,3 % vs. 3,6 %). Sixty-four comma three percent of the patients had found less satisfaction sexual intercourse. Dyspareunia was more frequent in case of episiotomy, perineal tear during the delivery and delivery assisted (vacuum). The patients, in 51 % of the cases, were themselves responsible for the refusal of the resumption of the sexual activity. The reasons of the refusal were of cultural and religious order and in touch with the absence of the return of menstruation in respectively 64,7 % and 54,9 % of the cases. CONCLUSION: The delivery had a negative impact on the resumption of couple sexual activity. Also, we noted a strong influence of the cultural and religious faiths on the sexual practice of the post-partum in the absence of reliable information. A particular attention should be concerned by the medical profession on the question of sexuality in the post-partum.

Bilan d'activite coelioscopique dans un hopital secondaire. cas de l'hopital general d'Ayame (Cote d'Ivoire)

Objectif. Etablir le bilan de l'activite colioscopique a l'Hopital General d'Ayame Methode. Il s'agissait d'une etude retrospective et descriptive qui s'etait realisee a l'hopital general d'Ayame et qui concernait tous les actes coelioscopiques effectues dans le service de gynecologie et d'obstetrique du 02 janvier 2013 au 30 juin 2014. Nous avions etudie les caracteristiques socio-demographiques des patientes; les indications; les actes operatoires (diagnostiques et therapeutiques) et la morbidite postoperatoire. Resultats. Il a ete realise 45 interventions percoelioscopiques; soit 21% de l'activite chirurgicale gynecologique de l'hopital. Les patientes avaient un age moyen de 33;1 ans avec des extremes de 24 et 44 ans. Il s'agissait en majorite de nulligestes et primigestes (66%); de nullipares (71%); de cadres et de niveau scolaire superieur (48;8%); residant a Abidjan (66;6%) et vivant maritalement (75%). L'infertilite et la seconde consultation post-myomectomie etaient les indications avec respectivement 67% (30 cas) et 33% (15 cas). Les constatations per operatoires pour infertilite etaient dominees par les adherences periannexielles; les kystes ovariens et les obstructions tubaires avec des frequences respectives de 33%; 27% et 23%. L'adhesiolyse et la kystectomie representaient les actes chirurgicaux les plus realises dans des proportions respectives de 53;3% (24 cas) et 13;3% (6 cas). Dans tous les cas de seconde consultation postmyomectomie; nous avions note des adherences parmi lesquelles predominaient les type II (60%). Aucune complication operatoire n'avait ete observee. Conclusion : la colioscopie est realisable en dehors des structures sanitaires de niveau tertiaire. Une mise a niveau du personnel pourrait ameliorer sa pratique

Analyse situationnelle de la mortalite perinatale au CHU de Bouake: resultats d'une etude portant sur le 1er semestre 2011

Contexte la mortalite perinatale represente une situation frequente malheureusement peu analysee; alors qu'elle constitue un parametre important dans l'evaluation de la qualite des soins. Objectif : Analyser la situation de la mortalite perinatale afin d'en preciser les facteurs favorisants. Patientes et methode : il s'agissait d'une etude retrospective descriptive exhaustive qui s'est deroulee a la maternite du CHU de Bouake; couvrant le 1er semestre de l'annee 2011. Elle a concerne tous les cas de deces feotaux enregistres directement dans le service apres accouchement et les enfants evacues en pediatrie qui y sont decedes. Nous avons analyse les caracteristiques epidemiologiques des meres; les circonstances de l'accouchement et les facteurs pouvant expliquer les deces fotaux Resultats : la mortalite perinatale representait 122 des accouchements de la periode d'etude. Cent vingt six enfants; soit 68;9; etaient morts pendant le travail. En pediatrie; vingt cinq nouveau-nes (13;70) y etaient morts. L'age moyen des patientes etait de 30 ans et la tranche d'age la plus representee etait celle des adolescentes avec un taux de 29;5. Il s'agissait aussi en majorite des femmes au foyer (86;3); primipares (26;8); avec antecedents de deces neonataux (38;8); un age variant de 25-34 ans (44;8); et provenant de la ville de Bouake (61;2). Les patientes etaient a 84;7 admises apres une evacuation dont les principaux motifs etaient la dystocie mecanique (24;6) et les metrorragies (13;66). L'accouchement des fotus morts s'etait realise par la cesarienne et par la laparotomie dans respectivement 39;3 et 14;8 des cas. Les indications de cesarienne etaient essentiellement l'epaule negligee et le syndrome de pre-rupture uterine a un taux identique de 20;83; et l'hematome retro-placentaire dans 19;4 des cas. En l'absence d'autopsie; les causes les plus probables des deces etaient le travail prolonge (38;89); l'hypertension arterielle (10;92).Conclusion : la mortalite perinatale est elevee au CHU de Bouake. Les causes sont essentiellement les dystocies mecaniques; dont la prise en charge adequate devrait permettre de reduire sa prevalence

Geographical Distribution of Cacao swollen shoot virus Molecular Variability in Côte d'Ivoire.

The discovery of new outbreaks caused by Cacao swollen shoot virus (CSSV) in Côte d'Ivoire in 2003, when this disease seemed to have been eradicated since the end of the 1950s in that country, casts doubt on the sustainability of Ivorian cocoa production. The aims of this study were, first, to carry out a molecular characterization of CSSV isolates from the main outbreaks in Côte d'Ivoire; second, determine their phylogenetic position in relation to isolates already discovered in Togo and Ghana; and, finally, study their geographical distribution to understand the dispersal of the virus. Additionally, this study was intended to enable the implementation and validation of a polyvalent molecular diagnosis assay for CSSV. Sequences analyses, corresponding to a fragment located at the 5' end of open reading frame (ORF)3 of the CSSV genome, revealed three new CSSV groups (D, E, and F) distinct from the A, B, and C groups already identified in Togo. Only group B was detected in all the outbreaks, whereas groups A and C were not identified in Côte d'Ivoire. In addition, a polymerase chain reaction diagnostic using the ORF3A F/R primer pair was polyvalent, because it enabled the detection of CSSV in 90% of the plots in all the cocoa regions analyzed by this study.

[Reality of the impact of pregnancy on sexuality. Results about review of 200 Ivoirians pregnant women]. / Réalité de l'impact de la grossesse sur la sexualité. Résultats d'une enquête auprès de 200 gestantes ivoiriennes.

BACKGROUND: Sexuality during pregnancy is a subject little approached during the antenatal follow-up while it sometimes constitutes a source of preoccupation for pregnant women for the preservation of the harmony of the couple. OBJECTIVE: Determine the opinions and the practices of women in sexuality during pregnancy. PATIENTS AND METHOD: Prospective transverse study of type knowledge practical attitude which took place from September 1st, 2008 to February 28th, 2009 at the General Hospital of South Abobo. It concerned 200 pregnant women who agreed to answer a pre-tested questionnaire, with their confirmed pregnancy. RESULTS: It is noted that 95,5% of pregnant women consider that it is possible to have sexual relations during the pregnancy. So 89% of them continue to have sexual relations during their pregnancy. The continuation of the sexual activity aims mostly at satisfying the pleasure of the woman and her partner (34,4%). Twenty-six percent of the questioned women consider that the sexual relations can have consequences on the pregnancy and that they would be responsible, for 60,9% of them, for miscarriages. Nine of 22 patients who observe a sexual abstinence during their pregnancy are afraid of hurting their fetus. Only 21,1% of pregnant women have appealed to the medical and paramedical staff to inquire on sexuality during pregnancy. They assert in 81,6% to have a decline of the libido and 54,7% find the sexual relations less satisfactory than before the pregnancy. CONCLUSION: The pregnancy influences negatively the sexual practices during the pregnancy. Pregnant women are badly informed and convey prejudices. The medical staff should offset for this deficit of information by daring to speak about it during the antenatal visits.

[Antituberculous retreatement in case of failure to category I regimen]. / Le retraitement antituberculeux en cas d'échec au régime de catégorie I.

INTRODUCTION: In Côte d'Ivoire, since April 2002, the antituberculous regime for category I patients (ARC-I) passed from 2RHZ/4RH to 2RHZE/4RH, without modification of the antituberculous regime for category II (ARC-II) for treatment of cases of the failures to respond to treatment with ARC-I (FARC-I) and patients with a relapse of tuberculosis (TR). The objective of this study was to determine the outcome of patients treated by ARC-II (2RHZES/1RHZE/5RHE). METHODOLOGY: This study was retrospective and compared outcomes during patient follow-up under ARC-II between 1999-2000 (period 1=267 cases) and 2004-2005 (period 2=434 cases). The ARC-II regime has been prescribed for 297 cases of FARC-I and 404 cases of TR. RESULTS: The failure rate of the ARC-II regime was estimated to be 11.98% during the first period compared to 5.53% during the second (P<0.001). Among FARC-I cases, therapeutic failure was estimated to 20.54% versus 5.92% in TR group (P<0,001). We noted a positive sputum smear among FARC-I : 16.16% at the second month, 13.13% to the third month and 20.54% at the fifth month versus 4.20% at the second month, 1.48% to the third month and 5.92% at the fifth month within TB cases (P<0.001). CONCLUSION: Management of failures to the ARC-I regime must be reviewed to prevent the development of multidrug resistant TB.

[Survey of general dental practitioners in Abidjan on the use of the operatory field in dentistry]. / Enquête auprès des omnipraticiens d'Abidjan sur l'utilisation du champ opératoire en dentisterie.

Rubber dam is a standard operatory field in operative dentistry and endodontics. It is undoubtedly the most certain way to insure the success of therapeutics acts. Its use by Abidjan practitioners has been the subject of our study which aim is to underline the place of rubber dam in daily practice. Our investigations show that rubber dam is totally absent from practitioners acts. Generally cotton rolls constitute the most frequent mean used by practitioners to isolate the tooth in operative dentistry and endodontics. Only 23.9% of them associate saliva sucking up. Some cases of aspiration and swallowing file by accident by patients have been highlighted. Those results lead us to recommend the systematic use of rubber dam as the mean of efficiency and security which cannot be ignored in our practice.

[Birth of a large fetus: epidemiologic aspects and analysis of factors of a case in an southern urban public health facility (Abidjan)]. / L'accouchement du gros foetus: aspects épidémiologiques et analyse des facteurs déterminants à propos d'une étude cas-témoin à la Formation sanitaire urbaine d'Abobo sud (Abidjan).

OBJECTIVE: to highlight the factors which influence the childbirth of the large foetus in order to propose adequate conduits. MATERIALS AND METHOD: it acted of a study case--witness who had been held on May 1, 2003 to April 31, 2004. We had included 108 cases of childbirth large foetus and 229 cases of childbirth of foetus of standard weight. The appreciation of the factors which influence the childbirth in these two groups was made thanks to the statistical analysis of the Chi 2 test; it had been considered to be significant with the threshold 5%. RESULTS: the parturients who were confined of the large foetus were referred than those of the group of the witnesses (p = 0,0421). The high way was observed in the event of large foetus (p = 0,0091). When the childbirth of the macrosomes was done by low way, one had noted more dystocie shoulders (0,0091) and more episiotomy (0,00479). The Caesarean for large foetus was carried out when the parturients were allowed in room of work in phase of latency of work (p = 0,028). Among the cesarized parturients, there were more first calf cows (p = 0,00532), young people and parturients whose size was between 150 and 170 cm (p = 0,00069) in the group of study. CONCLUSION: the childbirth of the large foetus is childbirth at the risk both for the mother the foetus. However it is necessary to be patient in front of an excessive uterine height evoking a large foetus. The indication of Caesarean should be posed only in primiparous, young parturients or teenagers, first calf cows of which size lower than 170 cm.