Chicken Incubation Conditions: Role in Embryo Development, Physiology and Adaptation to the Post-Hatch Environment.

The chicken hatching egg is a self-contained life-supporting system for the developing embryo. However, the post-hatch performance of birds depends on several factors, including the breeder management and age, egg storage conditions and duration before incubation, and the incubation conditions. Studies have determined the effect of incubation factors on chick post-hatch growth potential. Therefore, chick physical quality at hatch is receiving increasing attention. Indeed, although incubation temperature, humidity, turning and ventilation are widely investigated, the effects of several variables such as exposure of the embryo to high or low levels, time of exposure, the amplitude of variations and stage exposures on embryo development and post-hatch performance remain poorly understood. This review paper focuses on chick quality and post-hatch performance as affected by incubation conditions. Also, chick physical quality parameters are discussed in the context of the parameters for determining chick quality and the factors that may affect it. These include incubation factors such as relative humidity, temperature, turning requirements, ventilation, in ovo feeding and delay in feed access. All these factors affect chick embryo physiology and development trajectory and consequently the quality of the hatched chicks and post-hatch performance. The potential application of adapted incubation conditions for improvement of post-hatch performance up to slaughter age is also discussed. It is concluded that incubation conditions affect embryo parameters and consequently post-hatch growth differentially according to exposure time and stage of exposure. Therefore, classical physical conditions are required to improve hatchability, chick quality and post-hatch growth.

Effects of pre-incubation storage duration and nonventilation incubation procedure on embryonic physiology and post-hatch chick performance.

This study investigated the effects and possible interactions of storage and nonventilation during incubation for eggs from Sasso broiler breeder flock on pre- and post-hatch incubation results. A total of 1,260 Sasso eggs from a 58-wk-old broiler breeder flock were individually numbered, weighed and stored for 7 d or for 18 d in a climate-controlled room (16°C, 75% RH). After storage, eggs were weighed, and randomly assigned equally into 2 incubators. One of the incubators was ventilated (V) for the entire incubation and the second was nonventilated (NV) for the first 12 d. At d 18, the eggs were weighed, candled, and fertile eggs were transferred from the turning trays to hatching baskets. During the last 3 d of incubation, hatching eggs were checked individually every 3 h for hatching events and hatchability of fertile eggs. After pull out at d 21.5, post-hatch performances was determined until 1 wk of age. Results showed that, embryo weights from eggs in NV incubator was significantly higher (P < 0.05) in both stored eggs compared to those from eggs in ventilated incubator, but embryos from eggs stored for 18 d were smaller (P < 0.05) than those from eggs stored for 7 d. Hatchability was higher (P < 0.0001) in NV incubator compared to V incubator in both 7 d and 18 d stored eggs and an interaction was found between incubation ventilation and storage duration on both hatchability and embryonic mortality (P < 0.0001). Chick weights from NV incubator at 7 d post-hatch was greater (P = 0.0009) than those from V incubator. Serum Tri-iodothyronine (T3) and Thyroxin (T4) concentrations were significantly higher (P < 0.05) in NV compare to V group. It was concluded that the effect of long-term pre-incubation storage on embryonic physiology and post-hatch growth interacted significantly with incubation ventilation and that nonventilation can compensate for the negative effects of storage on some hatching and post-hatch performances.

Effects of egg storage duration on egg quality, metabolic rate, hematological parameters during embryonic and post-hatch development of guinea fowl broilers.

Considering the value of guinea fowl keets, successful incubation of eggs is particularly desirable in this poultry species. This study evaluated the effect of egg storage duration on egg quality, heat production, hematological parameters during embryonic development and post hatch performance of guinea fowl broilers. A total of 800 hatching eggs of guinea fowl were used for this study. Before incubation, 12 eggs per treatment were used to analyse egg quality. Then, eggs were numbered, weighed, and assigned to 2 treatment groups of 400 eggs each according to storage duration of 5, and 10 d at a temperature of 18°C. The eggs were set for incubation at 37.7°C and 55% relative humidity for 28 d in a forced-draft incubator. To determine heat production as a measure of metabolism, 60 eggs in each replicate were transferred to respiratory cages post hatch two 12 wk old guinea fowl were also used to determine heat production. CO2 and O2 were recorded to calculate heat production at internal pipping, hatch and at 12 wk of age. The hatched keets were reared for 12 wk and data were collected on feed intake, body weight and feed conversion ratio. Blood samples were collected at hatch and at 12 wk of age from 24 guinea fowls per treatment to analyze haematological parameters. The results showed that embryos and guinea fowls at 12 wks of age from eggs stored for 5 d had higher (P ˂ 0.05) heat production and body weights. However, a significant higher (P ˂ 0.05) level of basophile, eosinophils, and lymphocytes was observed in guinea fowls from 10 d storage egg. It was concluded that extended duration of egg storage negatively influenced the metabolic rate of embryos. It also impacted hematological parameters which may suggest influence on immune response during embryonic and post-hatch growth.

Trauma of the external genital in children: Emasculation, a paediatric case report.

An 8-year-old boy was brought to the paediatric surgery department having amputated his penis and both testicles during a road traffic accident. Examination of the perinea showed a complete amputation of penis, scrotum and testicles. We performed debridement and skin suture initially. The urethral orifice was catheterized by a 10 F Foley's catheter. I the herein case report, we discuss the incidence, management and complications of genital amputation in a young boy. Moreover, the existing literature in this subject is reviewed.

Effect of guinea fowl egg storage duration on embryonic and physiological parameters, and keet juvenile growth.

This study investigated the effects of guinea fowl hatching eggs storage time on embryo parameters and post-hatch juvenile growth. A total 1,800 eggs of guinea fowl were used. They were numbered, weighed, and divided into four groups of 450 eggs each according to storage time of 3, 7, 11, and 15 D before storage at a temperature of 18°C. Then, they were incubated at 37.7°C and 55% relative humidity for 28 D in a forced-draft incubator. Egg weight loss, albumen pH and weight, embryo weight, hatching events, and keet growth up to 7 D post-hatch were recorded. In addition, thyroid hormone and corticosterone levels were determined. The results indicate that during storage, relative egg weight loss increased with storage duration. However, albumen pH increased with storage time up to 11 D of storage and remained unchanged between 11 and 15 D. In addition, from 19 to 22 D of incubation, albumen weight was higher for eggs stored for 15 D compared to that of eggs stored for 3 to 11 D. But, from 16 D of incubation, embryos from eggs stored for 3 D grew faster than those from eggs stored for 7 to 15 D. Incubation durations up to internal pipping (IP), external pipping (EP), and hatching events increased with egg storage duration. At IP, corticosterone and triiodothyronine (T3) concentrations of eggs stored for 15 D had the lowest (P < 0.05) compared to those of eggs stored for 3 to 11 D. Moreover, the levels of thyroxine (T4) decreased with storage duration (P < 0.05). At hatch, corticosterone levels increased while T4 levels decreased with storage duration (P < 0.05). Also, hatchability decreased with egg storage duration. In addition, 7-day-old keets from eggs stored for 3 and 7 D had comparable weight and were heavier than those from eggs stored for 11 D. It was concluded that storage of guinea fowl hatching eggs more than 7 D negatively affects egg quality and subsequently depresses embryo and post-hatch growth.

Les complications de la circoncision au CHU de Yopougon

Circoncision masculine - méthodes

Surgical treatment of femoral diaphyseal fractures in children using elastic stable intramedullary nailing by open reduction at Yopougon Teaching Hospital.

INTRODUCTION: Elastic stable intramedullary nailing (ESIN) has transformed children's femoral shaft fracture treatment, but this technique requires an image intensifier. Without it, open reduction is used to check fracture reduction and pin passage. The aim of this study was to describe our techniques and to evaluate our results at the middle term. HYPOTHESIS: The open reduction and ESIN technique provides satisfactory results with few major complications. PATIENTS AND METHODS: This was a retrospective study that focused on femoral diaphyseal fractures treated in the pediatric surgery unit at Yopougon Teaching Hospital (Abidjan, Côte d'Ivoire) between January 2007 and December 2013. Twenty children older than 6 years of age who underwent open reduction and ESIN without image intensifier assistance were included. Functional outcomes were assessed using Flynn's criteria. Postoperative complications and sequelae were recorded. RESULTS: At the 16-month follow-up, the results were excellent in 11 (55%) cases, good in eight (40%), and poor in one (5%) case. The mean duration of surgery was 71min (range, 57-103 min). The mean time for bone healing was 11.6 weeks (range, 7-15 weeks) and the average time to nail removal was 6 months. Complications included wood infection (n=3), skin irritation (n=3), knee stiffness (n=2), malunion (n=3), scar (n=5), and leg length discrepancy (n=3). DISCUSSION: Open reduction and ESIN yielded satisfactory results with few major complications. This method could be an alternative in low-income countries where the image intensifier is often unavailable. LEVEL OF EVIDENCE: Level IV retrospective study.

Epidemiologie des anomalies congenitales dans les centres hospitaliers et universitaires d'Abidjan

Les anomalies congenitales constituent l'une des principales causes de la mortalite infantile dans le monde et particulierement dans les pays en developpement. Objectif principal etait de repertorier toutes les malformations observees dans les trois centres Hospitaliers Universitaires d'Abidjan et a l'institut de cardiologie et les Objectifs secondaires de : - Repertorier leurs caracteristiques epidemiologiques ; - Hierarchiser les pathologies malformatives en fonction de leurs frequences ; - Etablir la mortalite globale de ces pathologies malformatives ; - Etablir la letalite de chacune de ces pathologies malformatives. Patients et methode. Il s'agissait d'une etude retrospective et descriptive sur 10 ans. Les donnees etudiees etaient celles epidemiologiques repertoriees a partir des dossiers medicaux et registres dans les 3 CHU d'Abidjan. Les nouveau-nes de 0 a 28 jours traites pour une anomalie congenitale pendant la periode d'etude etaient la population etudiee. Les donnees ont ete traitees et analysees par les logiciels epi info et Excel. Resultats. De 1996-2006; 1496 nouveaux nes presentant 1589 anomalies congenitales ont ete recenses dans les centres hospitaliers universitaires d'Abidjan. Il s'agissait de nouveau-nes; de meres agees de moins de 35 ans dans 33 des cas; multigestes dans 20 des cas; multipares dans 18 des cas. Les grossesses ont ete regulierement suivies dans 22 des cas et une echographie obstetricale a ete effectuee dans 1 cas sur 4. Le diagnostic prenatal des anomalies congenitales a ete effectue dans 1;5 des cas. Le diagnostic des anomalies congenitales a ete fait dans 79 ;5 des cas a la naissance. Les anomalies congenitales observees etaient : Orthopediques : 33; Neurologiques : 18; Digestives : 14; Faciales : 12;5; Parietales : 12; Urogenitales : 10; Cardiaques : 0;5. - La mortalite globale etait de 52. Les malformations digestives; parietales et neurologiques sont les plus letales Conclusion. les anomalies congenitales existent et sont relativement importantes dans nos structures hospitalieres. Leur diagnostic prenatal reste faible. Elles sont dominees par les anomalies orthopediques. Leur mortalite reste elevee. La mortalite est grevee par une letalite plus importante des pathologies congenitales viscerales

Infections osteo-articulaires (IOA) des nourrissons de moins de 3 ans: particularites cliniques; bacteriologiques; therapeutiques et pronostiques

Contexte. Dans les pays industrialises; les IoA sont prises en charge tres rapidement au debut des manifestations cliniques contrairement aux pays en developpement ou elles sont traitees tard au stade subaigu ou chronique. L'objectif de cette etude etait de decrire les IAo chez les enfants de moins de 3 ans afin de definir leurs particularites cliniques; d'identifier les bacteries en cause. Patients et methode. Sur une periode de 11 ans; de Decembre 2002 a Janvier 2013; nous avons collige des dossiers d'Infections osteo Articulaires (IoA) d'enfants au Centre Hospitalier Universitaire de Yopougon Abidjan Cote d'Ivoire. Les donnees cliniques concernaient; le delai de consultation; les portes d'entrees; la symptomatologie clinique (signes generaux; signes fonctionnels; signes physiques); Les parametres etudies etaient cliniques; biologiques; bacteriologiques; therapeutiques et evolutifs. Resultats. Au total; nous avons collige 105 dossiers d'Infections osteo Articulaires (IoA) d'enfants de 0 a 36 mois. Les resultats de l'hemoculture ont ete positifs dans 22%; ceux de la ponction articulaire ont ete positifs dans 51;5%; ceux de la ponction metaphysaire dans 22% et ceux du prelevement de la porte d'entree dans 60% . Les bacteries isolees etaient; le staphylococcus aureus dans 54%; Salmonella dans 23%; Klebsiella pneumoniae dans 19%; Pyocianique dans 4%. L'antibiogramme a montre une sensibilite des bacteries isolees dans 100% des cas aux Aminosides; aux Fluoroquinolones; a la Fosfomycine; aux Imipenemes et aux Glycopeptides. La sensibilite aux Betalactamines etait de 60%. Conclusion. Il faut eviter les betalactamines en mono antibiotherapie de premiere ligne au cours des IAo chez les nourrissons de moins de 3 ans. Nous suggerons l'utilisation d'une mono antibiotherapie avec amoxicilline acide clavulanique qui vise l'elimination du Kingella Kingae premiere bacterie en cause au cours des infections orL des nourrissons mais aussi a cause de l'acide clavulanique pour eliminer les staphylocoques dores producteurs de betalactamase

[Lyell's syndrome occurring in three HIV-positive patients undergoing anti-tuberculous therapy]. / Syndrome de Lyell chez trois malades VIH (+) sous traitement antituberculeux.

We describe three cases of Lyell syndrome - toxic epidermal necrolysis - occurring in patients under the respiratory service of CHU de Cocody in Abidjan in the Ivory Coast, who were undergoing anti-tuberculous (TB) chemotherapy. All three were adult males who were HIV-positive, but none of them were receiving anti-retroviral treatment. They were on standard anti-TB treatment; rifampicin, isoniazid, pyazinamide and ethambutol. The lesions appeared early during their treatment for TB (14, 20 and 45 days). The patients were on no other medication that might have caused Lyell's syndrome except one who had been on cotrimoxasole for 6 months without complications. Despite admission to the acute care facility and appropriate care two of the three patients died.

[Total rectal prolapse in adults: report of 26 cases in the Ivory Coast]. / Le prolapsus rectal total de l'adulte: étude de 26 cas en milieu ivoirien.

Several studies are still in progress to improve the results of rectopexy for treatment of full thickness rectal prolapse in adults. The purpose of this study was to evaluate etiologic factors of full thickness rectal prolapse in Ivory Coast and to describe results in 26 patients treated surgically in Africa over a period of 10 years. There were 23 men with ages ranging from 30 to 45 years and 3 women with ages ranging from 45 to 50 years. All three women were multiparous. Related factors in the men were chronic constipation in 69.2%, diarrhea in 11.5%, and dysuria in 7.7%. Fifteen patients (59.7%) presented anal incontinence prior to surgery. Rectoscopy was performed in 23 cases and findings were always normal. Barium enema x-rays were performed in 3 cases and demonstrated dolicho-colon. The Orr-Loygue rectopexy procedure was performed in 23 patients and direct rectopexy in 3 patients. No operative deaths occurred and no recurrences have been observed with a follow-up of 24 months. Constipation developed following surgery in two patients and persisted in all patients who presented this symptom prior to surgery. Progressive improvement was observed in all fifteen patients with preoperative anal incontinence. Based on these results, our current management strategy includes determination of colonic transit time to select indications for direct rectopexy with sigmoidectomy.

[Perineal gangrene: report of 30 cases observed at Abidjan]. / Gangrene perineale: a propos de 30 cas observes a Abidjan.

Perineal gangrene may be classified as primary, e.g. Fournier's gangrene of the external genitals, or secondary due to locoregional injury. Perineal gangrene is still a topic of interest in tropical zones where mortality remains high despite current therapeutic modalities. The purpose of this retrospective study was to analyze etiologic, bacteriologic, and therapeutic data in 30 cases observed over a one-year period in a surgical setting in the Ivory Coast. There were 26 men and 4 women with a mean age of 43 years. All were treated by extensive removal of necrotic tissue in association with antimicrobial therapy for the likeliest agent and appropriate supportive measures. Colostomy was performed in 6 cases and drainage of the urinary tract was required in 11 cases. Anti-tetanus treatment was administered in all cases. Thirteen patients were HIV-positive, five were diabetic, and two presented chronic renal insufficiency. Perineal gangrene was classified as secondary in 17 cases and primary (Fournier's gangrene) in 13 cases. Bacteriologic study of puss and tissue specimens collected from necrotic areas demonstrated a mixed population of microbial organisms predominated by streptococcus A (n = 19) and Escherichia coli (n = 13). Positive diagnostic results were obtained in 5 of 20 hemocultures and in 8 of 11 cystobacteriologic tests. Eight patients died and nine presented complications including necrosis of the testicles, atrophy of the testicles, shock, and coma. Healing was obtained without skin grafting in 22 survival cases but 5 of them required secondary suturing. The mean duration of hospitalization was 45 days. In our experience, perineal gangrene led to severe consequences despite adequate management especially in HIV-infected patients. The treatment of choice is still extensive emergency surgical removal (repeated, if necessary) of necrotic tissue in association with adequate supportive measures and wide spectrum antimicrobial therapy.

Morsures de serpents : aspects epidemiologiques et protocole therapeutique

En 12 mois; 53 cas de morsures de serpents ont ete colliges. Le sexe masculin est le plus touche. Le tableau clinique est celui d'un syndrome inflammatoire localise au niveau des membres inferieurs; il est plus sensible a l'heparinate de sodium intraveineux qu'aux anti-flammatoires non steroidiens. La duree moyenne d'hospitalisation a ete de 2;87 jours. Le traitement a ete uniquement symptomatique et l'evolution favorable dans 100 pour cent des cas