RESUMO
We examined the ability of monocytes (MPhi) activated by bacterial products to alter epithelial physiology. Confluent monolayers of the T84 colonic epithelial cell line were grown on filter supports and then cocultured in the presence of human MPhi with or without the activating agents bacterial lipopolysaccharide and the bacterial tripeptide formyl-methionyl-leucyl-phenylalanine. After 24 or 48 h, monolayers were mounted in Ussing chambers where parameters of epithelial function were measured. Exposure to activated MPhi resulted in a significant increase (P < 0.05) in baseline short-circuit current (250% after 48 h) that was associated with enhanced secretion of Cl-. In addition, epithelial permeability was significantly increased as shown by reduced transepithelial resistance and increased flux of 51Cr-EDTA. Activated MPhi produced substantial amounts (approximately 3 ng/ml at 48 h) of tumor necrosis factor-alpha (TNF-alpha). TNF-alpha was identified as a key mediator acting via an autocrine mechanism to induce epithelial pathophysiology. Our data show that MPhi, when activated by common bacterial components, are potent effector cells capable of initiating significant changes in the transport and barrier properties of a model epithelium.
Assuntos
Mucosa Intestinal/citologia , Mucosa Intestinal/fisiologia , Macrófagos/fisiologia , Monócitos/fisiologia , Fator de Necrose Tumoral alfa/fisiologia , Adulto , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Técnicas de Cocultura , Colforsina/farmacologia , Colo , Feminino , Humanos , Mucosa Intestinal/efeitos dos fármacos , Lipopolissacarídeos/farmacologia , Ativação de Macrófagos , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Masculino , Pessoa de Meia-Idade , Monócitos/citologia , Monócitos/efeitos dos fármacos , N-Formilmetionina Leucil-Fenilalanina/farmacologia , Proteínas Recombinantes/farmacologia , Salmonella , Fator de Necrose Tumoral alfa/farmacologiaRESUMO
PURPOSE: Cicatricial conjunctivitis is an uncommon ocular finding with several possible origins. We report a patient whose obsessive-compulsive disorder involved constant self-induced mechanical trauma to the eyes, leading to bilateral cicatricial conjunctivitis. METHOD: Case report. RESULTS: Based on the history, physical examination, ocular surface scraping, and conjuctival biopsy, other causes of cicatrical conjunctivitis were ruled out, and a self-induced cause was confirmed. CONCLUSIONS: Inquiries pertaining to psychiatric history may be important in patients with cicatricial conjunctivitis.
