[Histone-antihistone interactions in the Escherichia coli-Tetrahymena pyriformis association].

Using the Escherichia coli-Tetrahymena pyriformis system, we revealed the involvement of bacterial antihistone activity and protozoan histones in interactions between pro- and eukaryotic microorganisms. Antihistone activity enhanced the viability of E. coli in association with T. pyriformis, according to our data on the dynamics of E. coli cell numbers. The strain with antihistone activity induced incomplete phagocytosis in the infusorians, resulting in cytological changes and ultrastructural alterations that indicated the retention of bacterial cells in phagosomes. Bacteria with antihistone activity located in the T. pyriformis cytoplasm influenced the eukaryotic nucleus. This manifested itself in macronucleus decompactization and a decrease in the average histone content in the population of infusorians. The data obtained suggest that protozoan histone inactivation by bacteria is one of the mechanisms involved in prokaryote persistence in associations with eukaryotic microorganisms.

[Anticarnosine activity of staphylococci as a criterion for assessment of their persistence potential].

The analysis on clinical material and the use of experimental models allowed to prove the role of anticarnosine activity of staphylococci in their persistence. Light and electronic microscopy revealed large destruction of ultrastructural eukaryote components and decreased proliferative activity in animals challenged with strains characterized by high anticarnosine activity. Adaptive mechanisms, providing dynamic equilibrium in "eukaryote--prokaryote" system, are described.

[Characterization of relationships between pro- and eukaryotic cells under the conditions of intracellular symbiosis of Staphylococcus aureus in the mucosa cells of the respiratory and digestive systems organs in terms of ecology and morphology]. / Ekologo-morfologicheskaia kharakteristika vzaimootnoshenii pro- i éukarioticheskikh kletok v usloviiakh vnutrikletochnogo simbioza Staphylococcus aureus v kletkakh slizistykh obolochek organov dykhatel'noi i pishchevaritel'noi sistem.

The ecological and morphological analysis of reorganization processes in the cell and tissue structures of the mucous membranes of the respiratory and digestive systems in rats under the conditions of their prolonged symbiotic interactions with staphylococci (in the body of rats as a whole, as well as on the model of organotypic cultures in diffusion chambers in vivo) was carried out with the use of light and electron microscopy, radioautography and morphometry. The morphological equivalents of phenotypic reactions in the cell elements of the mucous membranes of the respiratory and digestive systems (epitheliocytes, leimyocytes, endotheliocytes, macrophages and fibroblasts) under the conditions of staphylococcus persistence were revealed. Adaptive and reactive shifts in eukaryotic cells were manifested by an increased volume of nuclei and greater proportion of euchromatin and a decreased DNA-synthetic and proliferative activity. Microorganisms located inside the cells underwent ultrastructural reorganization.

[Structural and functional reorganization of staphylococci and air-bearing system following experimental intratracheal introduction of pathogen]. / Strukturno-funktsional'naia reorganizatsiia stafilokokkov i vozdukhonosnoi sistemy pri intratrakheal'nom vvedenii patogena v éksperimente.

Morphological changes in the cells of the mucous membrane of the tracheo-bronchial system and in staphylococci under the conditions of intratracheal infection were studied. Different forms of interaction between the host cell and staphylococci, reflecting the organ specific properties and a wide range of compensation and adaptation reactions of eukaryotic and prokaryotic cells were revealed with the use of electron microscopy.

[Structural and functional reorganization in epithelia of soft palate and bronchi cultured in vivo using F.M. Lazarenko method: the role of neuroendocrine factors]. / Strukturno-funktsional'naia reorganizatsiia épiteliev miagkogo neba i bronkhov v usloviiakh ikh kul'tivirovaniia v organizme po metodu F.M. Lazarenko: rol' neiroéndokrinnykh faktorov.

To define the range of histio- and organotypical capacities of rat soft palatine and bronchial epithelia, and to elucidate the nature of the effect of neuroendocrine factors of hypothalamic paraventricular factors upon them, the experiments were conducted by co-culturing mucosal fragments of oral and pharyngeal surfaces of soft palate and bronchi with the paraventricular nuclei-containing hypothalamic fragments using in vivo F.M. Lazarenko method. Soft palatine and bronchial epithelia were found to have similar histoblastic properties. The stimulating effect of hypothalamic factors, released paraventricular nuclei, on the processes of regeneration of epithelia and of connective tissue was noted.

[Fundamental and applied aspects of neuroendocrine regulation of pro- and eukaryote interactions]. / Fundamental'ye i prikladnye aspekty neiroéndokrinnoi reguliatsii vzaimodeistviia pro- i éukariot.

The paper presents the results of light, atomic-force, and electron microscopic studies of prokaryotes (various Staphylococcus aureus strains) and eukaryotes (Rat digestive and respiratory cells and tissues) in in vivo and in vivo interactions. It is concluded that hypothalamic nonapeptides play a modulating role in the persistence and symbiotic relations in the bacterium-host system and regulate cellular and tissue homeostasis through reparative histogenesis.

[Role of hypothalamic nonapeptides in interaction of prokaryotic and eukaryotic cells]. / Rol' gipotalamicheskikh nonapeptidov vo vzaimodeistvii pro- i éukarioticheskikh kletok.

The role of hypothalamic nonapeptides in the interaction of prokaryotic and eukaryotic cells was studied in the experimental setting. Nonapeptides were found to stimulate the adaptive and regenerative properties of eukaryotic cells and they are likely to have an antimicrobic effect on prokaryotic ones. The paper discusses the modulating role of nonapeptides in the establishment of symbiotic relations in the bacterial agent-host system.

[Effect of carnosine on the morphofunctional state of mucosal cells of the soft palate of rats in staphylococcal infection]. / Vliianie karnozina na morfofunktsional'noe sostoianie kletok slizistoi obolochki miagkogo neba krys pri stafilokokkovoi infektsii.

The influence of carnosine on the morphofunctional state of mucosal cells of the soft palate of rats in experimental staphylococcal infection has been studied. Morphological changes in muscular and connective tissue cells of the mucosa of the soft palate of rats have been found to occur. The suppressive effect of carnosine with respect to the pathogen and its positive influence on the processes of the regeneration of eukaryotic tissues.

[Biological importance of the anti-carnosine activity of bacteria]. / Biologicheskoe znachenie antikarnozinovoi aktivnosti bakterii.

The anticarnosine activity has been found to be widely spread among bacteria, the degree of its manifestation depending on the source of isolation. The fact that anticarnosine activity plays a certain role in the phenomenon of persistence has been proved on the model of experimental staphylococcal infection in rats.

[The histogenetic characteristics of the epithelial tissues of the mucosa of the pharyngeal and oral surfaces of the soft palate in ontogeny and under experimental conditions]. / Gistogeneticheskie osobennosti épitelial'nykh tkanei slizistykh obolochek glotochnoi i rotovoi poverkhnostei miagkogo neba v ontogeneze i v éksperimental'nykh usloviiakh.

Processes of the development and growth of the soft palate mucosas in ontogenesis and also proliferation, growth and differentiation of epithelial structures were studied in the experiment. The epithelium of the soft palate nasal surface was established to be more labile and to be characterized with polymorphism forming single-layer and pseudostratified structures. Under these conditions the oral surface epithelium keeps stratified structures firmly. On the basis of discovered differences of the epithelium biological qualities a suggestion is made on the different genetic origin of epithelial tissues of mucosas of the soft palate oral and pharyngeal surfaces.