RESUMO
Using the Escherichia coli-Tetrahymena pyriformis system, we revealed the involvement of bacterial antihistone activity and protozoan histones in interactions between pro- and eukaryotic microorganisms. Antihistone activity enhanced the viability of E. coli in association with T. pyriformis, according to our data on the dynamics of E. coli cell numbers. The strain with antihistone activity induced incomplete phagocytosis in the infusorians, resulting in cytological changes and ultrastructural alterations that indicated the retention of bacterial cells in phagosomes. Bacteria with antihistone activity located in the T. pyriformis cytoplasm influenced the eukaryotic nucleus. This manifested itself in macronucleus decompactization and a decrease in the average histone content in the population of infusorians. The data obtained suggest that protozoan histone inactivation by bacteria is one of the mechanisms involved in prokaryote persistence in associations with eukaryotic microorganisms.
Assuntos
Escherichia coli/crescimento & desenvolvimento , Histonas/fisiologia , Tetrahymena pyriformis/química , Tetrahymena pyriformis/microbiologia , Animais , Contagem de Colônia Microbiana , Fagocitose , Fagossomos/microbiologiaRESUMO
The analysis on clinical material and the use of experimental models allowed to prove the role of anticarnosine activity of staphylococci in their persistence. Light and electronic microscopy revealed large destruction of ultrastructural eukaryote components and decreased proliferative activity in animals challenged with strains characterized by high anticarnosine activity. Adaptive mechanisms, providing dynamic equilibrium in "eukaryote--prokaryote" system, are described.
Assuntos
Fatores Biológicos/metabolismo , Carnosina/antagonistas & inibidores , Carnosina/metabolismo , Infecções por Bactérias Gram-Positivas/microbiologia , Staphylococcaceae/metabolismo , Adolescente , Animais , Carnosina/análise , Criança , Humanos , Masculino , Camundongos , Camundongos Endogâmicos CBA , Ratos , Staphylococcaceae/crescimento & desenvolvimento , Staphylococcaceae/patogenicidade , VirulênciaRESUMO
The ecological and morphological analysis of reorganization processes in the cell and tissue structures of the mucous membranes of the respiratory and digestive systems in rats under the conditions of their prolonged symbiotic interactions with staphylococci (in the body of rats as a whole, as well as on the model of organotypic cultures in diffusion chambers in vivo) was carried out with the use of light and electron microscopy, radioautography and morphometry. The morphological equivalents of phenotypic reactions in the cell elements of the mucous membranes of the respiratory and digestive systems (epitheliocytes, leimyocytes, endotheliocytes, macrophages and fibroblasts) under the conditions of staphylococcus persistence were revealed. Adaptive and reactive shifts in eukaryotic cells were manifested by an increased volume of nuclei and greater proportion of euchromatin and a decreased DNA-synthetic and proliferative activity. Microorganisms located inside the cells underwent ultrastructural reorganization.
Assuntos
Brônquios/microbiologia , Mucosa Bucal/microbiologia , Mucosa Nasal/microbiologia , Infecções Estafilocócicas/patologia , Staphylococcus aureus/fisiologia , Adaptação Fisiológica , Animais , Autorradiografia , Brônquios/patologia , DNA/biossíntese , Cultura em Câmaras de Difusão , Modelos Animais de Doenças , Eucromatina/metabolismo , Masculino , Microscopia Eletrônica , Mucosa Bucal/patologia , Mucosa/microbiologia , Mucosa/patologia , Mucosa Nasal/patologia , Ratos , Staphylococcus aureus/isolamento & purificação , SimbioseRESUMO
Morphological changes in the cells of the mucous membrane of the tracheo-bronchial system and in staphylococci under the conditions of intratracheal infection were studied. Different forms of interaction between the host cell and staphylococci, reflecting the organ specific properties and a wide range of compensation and adaptation reactions of eukaryotic and prokaryotic cells were revealed with the use of electron microscopy.
Assuntos
Brônquios/patologia , Infecções Respiratórias/patologia , Infecções Estafilocócicas/patologia , Staphylococcus aureus , Traqueia/patologia , Animais , Brônquios/microbiologia , Modelos Animais de Doenças , Masculino , Microscopia Eletrônica , Especificidade de Órgãos , Ratos , Mucosa Respiratória/microbiologia , Mucosa Respiratória/patologia , Infecções Respiratórias/microbiologia , Staphylococcus aureus/isolamento & purificação , Staphylococcus aureus/ultraestrutura , Traqueia/microbiologiaRESUMO
To define the range of histio- and organotypical capacities of rat soft palatine and bronchial epithelia, and to elucidate the nature of the effect of neuroendocrine factors of hypothalamic paraventricular factors upon them, the experiments were conducted by co-culturing mucosal fragments of oral and pharyngeal surfaces of soft palate and bronchi with the paraventricular nuclei-containing hypothalamic fragments using in vivo F.M. Lazarenko method. Soft palatine and bronchial epithelia were found to have similar histoblastic properties. The stimulating effect of hypothalamic factors, released paraventricular nuclei, on the processes of regeneration of epithelia and of connective tissue was noted.
Assuntos
Brônquios/ultraestrutura , Neuropeptídeos/metabolismo , Palato Mole/ultraestrutura , Regeneração , Animais , Brônquios/fisiologia , Brônquios/transplante , Epitélio/fisiologia , Epitélio/transplante , Epitélio/ultraestrutura , Hipotálamo/metabolismo , Masculino , Microscopia Eletrônica , Técnicas de Cultura de Órgãos/métodos , Palato Mole/fisiologia , Palato Mole/transplante , Núcleo Hipotalâmico Paraventricular/metabolismo , Ratos , Transplante AutólogoRESUMO
The paper presents the results of light, atomic-force, and electron microscopic studies of prokaryotes (various Staphylococcus aureus strains) and eukaryotes (Rat digestive and respiratory cells and tissues) in in vivo and in vivo interactions. It is concluded that hypothalamic nonapeptides play a modulating role in the persistence and symbiotic relations in the bacterium-host system and regulate cellular and tissue homeostasis through reparative histogenesis.
Assuntos
Células Eucarióticas/metabolismo , Células Eucarióticas/microbiologia , Hipotálamo/metabolismo , Hipotálamo/microbiologia , Ocitocina/metabolismo , Infecções Estafilocócicas/microbiologia , Animais , Masculino , RatosRESUMO
The role of hypothalamic nonapeptides in the interaction of prokaryotic and eukaryotic cells was studied in the experimental setting. Nonapeptides were found to stimulate the adaptive and regenerative properties of eukaryotic cells and they are likely to have an antimicrobic effect on prokaryotic ones. The paper discusses the modulating role of nonapeptides in the establishment of symbiotic relations in the bacterial agent-host system.
Assuntos
Células Eucarióticas/fisiologia , Hipotálamo/metabolismo , Células Procarióticas/fisiologia , Vasopressinas/fisiologia , Animais , Células Eucarióticas/ultraestrutura , Humanos , Fagocitose/fisiologia , Células Procarióticas/ultraestruturaRESUMO
The influence of carnosine on the morphofunctional state of mucosal cells of the soft palate of rats in experimental staphylococcal infection has been studied. Morphological changes in muscular and connective tissue cells of the mucosa of the soft palate of rats have been found to occur. The suppressive effect of carnosine with respect to the pathogen and its positive influence on the processes of the regeneration of eukaryotic tissues.
Assuntos
Carnosina/uso terapêutico , Dipeptídeos/uso terapêutico , Mucosa Bucal/efeitos dos fármacos , Palato Mole/efeitos dos fármacos , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus aureus/metabolismo , Animais , Células Cultivadas , Tecido Conjuntivo/efeitos dos fármacos , Tecido Conjuntivo/microbiologia , Tecido Conjuntivo/patologia , Morfogênese/efeitos dos fármacos , Mucosa Bucal/microbiologia , Mucosa Bucal/patologia , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/microbiologia , Músculo Esquelético/patologia , Palato Mole/microbiologia , Palato Mole/patologia , Ratos , Regeneração/efeitos dos fármacos , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos dos fármacosRESUMO
The anticarnosine activity has been found to be widely spread among bacteria, the degree of its manifestation depending on the source of isolation. The fact that anticarnosine activity plays a certain role in the phenomenon of persistence has been proved on the model of experimental staphylococcal infection in rats.
Assuntos
Bactérias/patogenicidade , Carnosina/antagonistas & inibidores , Dipeptídeos/antagonistas & inibidores , Animais , Células Epiteliais/citologia , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/microbiologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Escherichia coli/metabolismo , Escherichia coli/patogenicidade , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/metabolismo , Infecções por Escherichia coli/microbiologia , Infecções por Klebsiella/tratamento farmacológico , Infecções por Klebsiella/metabolismo , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/isolamento & purificação , Klebsiella pneumoniae/metabolismo , Klebsiella pneumoniae/patogenicidade , Mucosa Nasal/citologia , Mucosa Nasal/efeitos dos fármacos , Mucosa Nasal/microbiologia , Ratos , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/metabolismo , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/isolamento & purificação , Staphylococcus aureus/metabolismo , Staphylococcus aureus/patogenicidade , Staphylococcus epidermidis/efeitos dos fármacos , Staphylococcus epidermidis/isolamento & purificação , Staphylococcus epidermidis/metabolismo , Staphylococcus epidermidis/patogenicidadeRESUMO
Processes of the development and growth of the soft palate mucosas in ontogenesis and also proliferation, growth and differentiation of epithelial structures were studied in the experiment. The epithelium of the soft palate nasal surface was established to be more labile and to be characterized with polymorphism forming single-layer and pseudostratified structures. Under these conditions the oral surface epithelium keeps stratified structures firmly. On the basis of discovered differences of the epithelium biological qualities a suggestion is made on the different genetic origin of epithelial tissues of mucosas of the soft palate oral and pharyngeal surfaces.