Seawater physics and chemistry along the Med-SHIP transects in the Mediterranean Sea in 2016.

The Mediterranean Sea has been sampled irregularly by research vessels in the past, mostly by national expeditions in regional waters. To monitor the hydrographic, biogeochemical and circulation changes in the Mediterranean Sea, a systematic repeat oceanographic survey programme called Med-SHIP was recommended by the Mediterranean Science Commission (CIESM) in 2011, as part of the Global Ocean Ship-based Hydrographic Investigations Program (GO-SHIP). Med-SHIP consists of zonal and meridional surveys with different frequencies, where comprehensive physical and biogeochemical properties are measured with the highest international standards. The first zonal survey was done in 2011 and repeated in 2018. In addition, a network of meridional (and other key) hydrographic sections were designed: the first cycle of these sections was completed in 2016, with three cruises funded by the EU project EUROFLEETS2. This paper presents the physical and chemical data of the meridional and key transects in the Western and Eastern Mediterranean Sea collected during those cruises.

Microplastic ingestion and its effects οn sea urchin Paracentrotus lividus: A field study in a coastal East Mediterranean environment.

Microplastics (MPs) are recognized as an increasing threat to the marine environment, but little is known about their effects on benthic organisms, including sea urchins, when ingested. For this purpose, wild sea urchins (P. lividus) and seafloor sediment samples were investigated across three coastal areas of Zakynthos Island (Ionian Sea), each exposed to different anthropogenic pressures, revealing a consistent pattern in MP abundance, shape, and color. Biomarkers related to oxidative stress, neurotoxicity, and genotoxicity showed no significant effects of MP ingestion in the sea urchins, except for a positive correlation between GST activity and ingested MPs, suggesting a possible activation of their detoxification system in response to MP ingestion. While MP concentrations in sea urchins and sediments were within the low range reported in the global literature, it remains crucial to conduct further investigations in areas with MP pollution approaching predicted levels to fully comprehend the potential effects of MP pollution on marine organisms.

Thirty years of a bottom oxygen depletion-renewal cycle in the coastal yet deep environment of the West Saronikos Gulf (Greece): Its drivers and the impact on the benthic communities.

In the period 1987-2017, a series of physical and chemical measurements related to oxygen variability at a trough area with a maximum depth of ~420 m in the West Saronikos Gulf, reveal the following: In the early 90s, deep winter mixing occurred resulting in an oxygenation down to ~420 m followed by an oxygen decline. This decline reached near-bottom hypoxic conditions (O2 < ~62 µM (µmol/L)) after 1998, while a denitrification phase occurred after 2000 and a complete bottom anoxia in 2005. In June 2012, an oxygenation down to ~350 m was detected that most likely occurred in winter 2012. The 2012 oxygenation raised the until-then anoxic bottom concentrations to hypoxic ones in the years towards 2017 via vertical diffusive oxygen transfer. Observations of the benthic communities during the hypoxia, severe hypoxia (O2 < ~15 µM) and oxygen recovery phases showed a peak of opportunists in the hypoxia and a long faunal depletion in the severe hypoxia phases. A reversal in the benthic community structure appeared after the oxygenation of 2012 with the (re)appearance of opportunists while, in 2017, the community showed signs of retreat to earlier stages. The main anthropogenic pressure that could tentatively affect the oxygen concentration in the study area is posed by the Athens treated-sewage outfall at ~40 km away from the trough, which inputs organic matter into the Saronikos Gulf through effluent water of reduced salinity that, in addition, may alter the stratification opposing the vertical mixing. We show that the treated sewage output had no influence on a) the stratification, b) the particulate and dissolved organic carbon and c) the sewage-derived organic matter. Instead, the long-term dissolved oxygen variability with the deep renewal events was mostly driven by the large-scale atmosphere-ocean conditions (heat exchange and evaporation-minus-precipitation budget) that determine the hydrographic characteristics and the winter mixing.

Responses of Free-Living Planktonic Bacterial Communities to Experimental Acidification and Warming.

Climate change driven by human activities encompasses the increase in atmospheric CO2 concentration and sea-surface temperature. Little is known regarding the synergistic effects of these phenomena on bacterial communities in oligotrophic marine ecosystems that are expected to be particularly vulnerable. Here, we studied bacterial community composition changes based on 16S rRNA sequencing at two fractions (0.1-0.2 and >0.2 µm) during a 10- day fully factorial mesocosm experiment in the eastern Mediterranean where the pH decreased by ~0.3 units and temperature increased by ~3 °C to project possible future changes in surface waters. The bacterial community experienced significant taxonomic differences driven by the combined effect of time and treatment; a community shift one day after the manipulations was noticed, followed by a similar state between all mesocosms at the third day, and mild shifts later on, which were remarkable mainly under sole acidification. The abundance of Synechococcus increased in response to warming, while the SAR11 clade immediately benefited from the combined acidification and warming. The effect of the acidification itself had a more persistent impact on community composition. This study highlights the importance of studying climate change consequences on ecosystem functioning both separately and simultaneously, considering the ambient environmental parameters.

Anthropogenic litter in freshwater bodies and their estuaries: an empirical analysis in Lesvos, Greece.

The last decade increasing attention to litter accumulation in the marine environment revealed the issue of the terrestrial fluxes of plastic pollution into the oceans. However, limited studies investigate the macro-litter abundance and composition particularly in freshwater ecosystems and the adjusted coastal environment. In this study, we conducted an empirical analysis of anthropogenic litter (AL) in two freshwater streams and their estuaries of Lesvos island (Greece), strongly affected by the waste mismanagement in the Moria refugee camp. A total of 1,622 litter items were collected from five sites during the field campaign between the 3rd and 6th of June 2019. Among these, artificial polymer items (mainly drink bottles, single-use plastic bags, cutlery, and trays) irrespective of the site location constituted the major part ranging from 46 to 91% of total AL by mass. Drink bottles and plastic cutlery are found in higher percentage at Moria camp river and its estuary, while packaging and bag litter are more abundant at upstream sites. The operation of the camp has increased the AL abundance 13 times more than the reference site, causing severe threat to human health and to freshwater and marine environment. It is therefore important to improve the waste management system at (inter)national (change in production and packaging policy) and local scale (introducing sustainable solutions to refugee camp design) by promoting recycling and circular economy projects to diminish AL pollution.

Metal and metallothionein levels in zooplankton in relation to environmental exposure: spatial and temporal variability (Saronikos Gulf, Greece).

Metal and metallothionein (MT) in mixed zooplankton were investigated as means of monitoring metal availability regarding environmental exposure. Spatial and temporal variability of Cd, Cu, Ni, Zn, Fe, Mn and Pb in zooplankton and seawater were studied in Saronikos Gulf (Aegean Sea, Eastern Mediterranean), once every second month during an annual cycle (2011-2012). Particulate organic carbon and chlorophyll α were also measured in seawater samples. Median zooplankton metal concentrations were 0.65, 32.4, 7.1, 864, 1420, 40.2 and 26.8 µg g-1 dw for Cd, Cu, Ni, Zn, Fe, Mn and Pb, respectively, and 109 µg g-1 ww for MTs. Metal levels in zooplankton and MTs were higher at sites influenced by human-derived pressures. Additionally, metal concentrations in pelagic fish flesh from the Greek MED-POL data base were used for bioconcentration and biomagnification factors calculation. Bioconcentration from water to zooplankton was higher than metal transfer from either seston to zooplankton or zooplankton to fish.

Coccolithophore community response to ocean acidification and warming in the Eastern Mediterranean Sea: results from a mesocosm experiment.

Mesocosm experiments have been fundamental to investigate the effects of elevated CO2 and ocean acidification (OA) on planktic communities. However, few of these experiments have been conducted using naturally nutrient-limited waters and/or considering the combined effects of OA and ocean warming (OW). Coccolithophores are a group of calcifying phytoplankton that can reach high abundances in the Mediterranean Sea, and whose responses to OA are modulated by temperature and nutrients. We present the results of the first land-based mesocosm experiment testing the effects of combined OA and OW on an oligotrophic Eastern Mediterranean coccolithophore community. Coccolithophore cell abundance drastically decreased under OW and combined OA and OW (greenhouse, GH) conditions. Emiliania huxleyi calcite mass decreased consistently only in the GH treatment; moreover, anomalous calcifications (i.e. coccolith malformations) were particularly common in the perturbed treatments, especially under OA. Overall, these data suggest that the projected increase in sea surface temperatures, including marine heatwaves, will cause rapid changes in Eastern Mediterranean coccolithophore communities, and that these effects will be exacerbated by OA.

Biomarkers of Exposure to Chemical Contamination in the Commercial Fish Species Lepidopus caudatus (Euphrasen, 1788): A Particular Focus on Plastic Additives.

In recent years, the Mediterranean Sea has become an accumulation zone for waste generated by the 22 countries bordering its shores. Although the effects of plastic litter on the marine environment and on organisms have recently been studied in other areas, further information is needed for the Mediterranean Sea and, in particular, about plastics additives inputs and interactions with the biota and the trophic network, such as phthalates and bisphenol A. Plastic material production, use and disposal contribute also to the release of heavy metals into the environment, such as mercury (Hg), often used during the production of chlorine, the primary ingredient in PVC, lead (Pb) and cadmium (Cd), which are used as stabilizers in PVC and leach out of products during use and disposal. Our research aims to evaluate phthalates, bisphenol A and heavy metals contamination in Lepidopus caudatus (Pisces, Trichiuridae), which could be considered as a potential sentinel species. For the evaluation of toxicological effects, we evaluated the expression of vitellogenin and metallothioneins 1. In all samples analyzed, we have not found microplastics in the gastrointestinal tract but chemical analysis revealed the presence of high content of phthalates, and in particular high quantities of DIDP, DEHP, bis-benzylester phthalate, bis-butyl ester phthalate and mono-N-butyl ester phthalate in different organs. Instead, trace elements detected in tissue revealed a trend of concentrations generally higher in liver and intestine than gill and muscle tissues. Immunohistochemical analysis for anti-metallothionein 1 antibody showed a strong positivity of liver cells, both in females and males. Analysis for the anti-vitellogenin antibody showed in females a strong positivity both in the liver cells and in the gonads, in male specimens was found to be always negative except for a specimen, in which it was highlighted a positivity in some areas of the liver and of the gonad.

Coccolithophore community response along a natural CO2 gradient off Methana (SW Saronikos Gulf, Greece, NE Mediterranean).

A natural pH gradient caused by marine CO2 seeps off the Methana peninsula (Saronikos Gulf, eastern Peloponnese peninsula) was used as a natural laboratory to assess potential effects of ocean acidification on coccolithophores. Coccolithophore communities were therefore investigated in plankton samples collected during September 2011, September 2016 and March 2017. The recorded cell concentrations were up to ~50 x103 cells/l, with a high Shannon index of up to 2.8, along a pH gradient from 7.61 to 8.18, with values being occasionally <7. Numerous holococcolithophore species represented 60-90% of the surface water assemblages in most samples during September samplings. Emiliania huxleyi was present only in low relative abundances in September samples, but it dominated in March assemblages. Neither malformed nor corroded coccolithophores were documented. Changes in the community structure can possibly be related to increased temperatures, while the overall trend associates low pH values with high cell densities. Our preliminary results indicate that in long-termed acidified, warm and stratified conditions, the study of the total coccolithophore assemblage may prove useful to recognize the intercommunity variability, which favors the increment of lightly calcified species such as holococcolithophores.