The Effect of Carbamazepine on Performance, Carcass Value, Hematological and Biochemical Blood Parameters, and Detection of Carbamazepine and Its Metabolites in Tissues, Internal Organs, and Body Fluids in Growing Rabbits.

Antiepileptic drugs (e.g., carbamazepine; CBZ) are widely prescribed for various conditions beyond epilepsy, including neurologic and psychiatric disorders. These medications can have both favorable and unfavorable impacts on mood, anxiety, depression, and psychosis. CBZ has been found at low concentrations (in the unit of nanograms per liter) in rivers, surface water, and even drinking water. As a result, when reclaimed wastewater is used for irrigation in agricultural ecosystems, CBZ can be reintroduced into the environment. That is why we tested different doses of CBZ in rabbits' feed as the meat is consumed in every community, has no religious barriers, and the potential risk of consuming meat which has been exposed to CBZ treatment is not known. Also, the evidence of the effect of CBZ on rabbits is missing. Mainly, the CBZ doses affected the count of leukocytes and other blood traits, meaning the higher the dose, the higher the reduction. Moreover, there were only low amounts of CBZ in rabbits' meat or tissues when they were exposed to the treatment.

Effect of Hen Genotype and Laying Time on Egg Quality and Albumen Lysozyme Content and Activity.

According to research, egg quality and lysozyme content are influenced by a number of factors, which are mostly known in the case of commercial hybrids, while in breeds included in genetic resources conservation programmes, new research results in this regard are emerging. The aim of the study was to determine the effect of egg laying time and genotype of selected Polish native breeds of hens on egg quality and lysozyme content and activity in the albumen. The study material consisted of eggs collected from four strains of laying hens included in the Polish conservation programme, i.e., Green-legged Partridge (Z-11), Yellow-legged Partridge (Z-33), Rhode Island Red (R-11) and Leghorn (H-22). At week 56, 28 eggs were randomly collected at 7:00 and 13:00 h from each breed of hen and assessed for quality. Laying time influenced certain egg quality traits. Eggs laid by hens in the morning were characterised by 1.7 g lower total weight and albumen weight, 2.4 pores/cm2 higher number of shell pores, 0.15 higher albumen pH values and 0.17 lower yolk pH values compared to those laid in the morning. The time of laying did not affect the level and activity of lysozyme in the albumen. A significant negative correlation was found between eggshell traits and albumen height, and between Haugh unit and lysozyme content and activity in the albumen. The studied egg quality traits were more influenced by genotype than by the egg-laying time.

Effects of Genotype and Housing System on Rabbit Does' Aggressive Behaviors and Injuries in Smallholding Conditions.

The objective of the study was to investigate the effects of housing (deep litter + concrete floor vs. deep litter + ground soil with the possibility to dig burrows), and genotype (Mecklenburg or Hyplus) on aggressive behaviour, social contacts, does' and kits' injuries, and progeny mortality. Twelve groups of six rabbit does (n = 72) were assigned to four treatments (two housing systems and two genotypes). Aggressive behaviour of does, number of injuries on does and kits, and postnatal kit mortality were recorded. The effects of housing and genotype were tested using multivariate GLMM Models. We found that the housing treatment in interaction with the genotype had a significant effect on aggressive behaviours in group housed does (F3,12 = 14.34, p = 0.0003), where the lowest incidence of aggression was in Mecklenburg does housed on ground soil. Reduced aggression was reflected in a lower number of injuries in does (F3,68 = 10.51, p < 0.0001), number of injuries in kits, and kit mortality (F3,1 = 4.59, p < 0.0001, F3,54 = 43.94, p < 0.0001). The results indicate that the proper combination of genotype and housing should be carefully considered for breeding to reduce aggression and injury in group housed does.

The effect of housing system on rabbit growth performance, carcass traits, and meat quality characteristics of different muscles.

The present study analysed the effect of housing system (caged versus penned) on the growth performance, carcass traits, and meat quality of rabbits. The physiochemical quality of five muscles was evaluated, together with the chemical composition and fatty acid profile of the meat from the hind leg. Sensory properties of the longissimus lumborum (LL) was also assessed. The LL yields were higher in caged rabbits. According to the results, meat from rabbits raised in cages had higher b* values for the LL (i.e., meat was yellower) than rabbits raised in pens. In addition, the quadriceps femoris from penned rabbits had higher a* and b* values (i.e., were redder and yellower) than caged rabbits. The MUFAs (18:1 n-9 and 20:1 n-9) and ash contents of the hind leg meat were higher in caged rabbits. In conclusion, while housing system influenced the physiochemical traits of rabbit meat, the sensory properties were not influenced.

Laying hens under smallholder conditions: laying performance, growth and bone quality of tibia and femur including essential elements.

The study aimed to assess laying performance, growth rate, and bone quality properties of tibia and femur bones of various genotypes of laying hens, including determining essential element composition at the end of the laying cycle in smallholder conditions. The study included three genotypes of laying hens; Czech golden spotted (CGS), White Leghorn (LE) and Dominant Partridge D300 (D300) hens. In total, 180 hens (60/genotype) were used in 3 replications (20 hens/replication). The eggs were collected to determine egg lay and hen-day egg production. Additionally, feed consumption was recorded to determine feed consumption per day or egg, resp. The mortality rate was recorded. Hens were individually weighed every 10 wk to analyze the growth performance and body weight changes during the laying cycle. The differences in performance characteristics were observed as significant in all studied parameters. The bone quality analysis consisted of the determination of bone weight, length, width, and fracture toughness. Furthermore, dry matter, ash, and selected elements, which included boron (B), calcium (Ca), cadmium (Cd), copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn), sodium (Na), phosphorus (P), lead (Pb), and zinc (Zn) were assessed. Regarding the results of tibia and femur bones, the effect of genotype was determined as significant in all evaluated properties. In terms of element composition, all evaluated elements significantly differed among the genotypes in the tibia (with one exception of Cu) and in the femur (with one exception of Cd). In conclusion, our results showed that hens' performance, production quality, mortality and bone properties significantly differed among genotypes under smallholder conditions. Thus, every genotype needs to be carefully considered, when the rearing conditions are set.

The Effect of Housing System and Gender on Relative Brain Weight, Body Temperature, Hematological Traits, and Bone Quality in Muscovy Ducks.

The study was conducted during the summer season (June-August 2020). Two hundred sixty-four 5-week-old sexed Muscovy ducklings were randomly divided into four equal experimental groups by housing system and by gender. Each group had three replicates (22 birds/replicate) in a randomized design experiment. Regarding the hematological traits, the volume of leukocytes was higher in the D group (by 0.34 × 109/L; p < 0.05) than in the S group. Furthermore, body temperature was found to be higher in ducks (by 0.84 °C; p < 0.05) and in the D group (by 0.5 °C; p < 0.05) in comparison with drakes and birds from the S group. Considering relative brain weight, drakes had higher values than ducks (by 0.56 g; p < 0.05), and birds from the S group also manifested higher values (by 0.78 g; p < 0.05). In terms of bone quality, there were no differences in studied parameters of tibia and femur bones regarding housing systems. The results provide valuable evidence of differences in the fattening of intensively bred Muscovy ducks within the housing system but also regarding gender.

Changes in Fatty Acids Profile, Health Indices, and Physical Characteristics of Organic Eggs from Laying Hens at the Beginning of the First and Second Laying Cycles.

The present study compared the fatty acid profile and some physical parameters of eggs from hens reared according to the organic system at the beginning of the first and second laying cycle. A total of 1080 eggs were analysed at the beginning of the first (from the 28th to 30th week of age) and the second (from the 78th to 80th week of age) laying cycle. It was found that the hen ages influenced the egg weight, egg surface area, yolk proportion, and eggshell colour. Albumen and eggshell proportion, albumen, yolk index, Haugh unit score, and eggshell strength were lower in eggs from older hens compared with those produced from younger layers. Monounsaturated fatty acids were found in higher amounts than saturated fatty acids and polyunsaturated fatty acids in egg yolks of eggs from layers only at the beginning of the second laying cycle. The PUFAn-6/n-3 ratio, saturation, atherogenic, and thrombogenic indices were significantly lower in the egg yolks from older hens compared to younger layers. These findings (regarding the eggs from the older ones) prove that it is practical to utilize them in the organic farming system during a period of two years.

Guinea fowl (Numida meleagris) eggs and free-range housing: a convenient alternative to laying hens' eggs in terms of food safety?

The aim of this study was to evaluate the impact of the genotype (guinea fowl, native breed Leghorn, and commercial hybrid hens), storage time (0, 14, 28 d) and storage temperature (fresh, 5, 20°C) on eggshell quality traits and microbiological contamination of eggshell, eggshell membranes, and albumen. A total of 150 hens (50 hens per genotype-divided into 2 equal groups because of the results replication) were used. There were 150 eggs (50 per genotype) used for microbial analysis and 600 eggs used for the analysis of eggshell quality. The effects of genotype, storage time, and storage temperature were observed. Moreover, interactions between these factors were calculated. The significant effect of genotype (P = 0.0001) was found in egg weight, in all observed parameters of eggshell quality (proportion, thickness, strength, surface, and index), eggshell contamination of Escherichia coli (EC) and total number of micro-organisms (TNM), penetration of TNM into eggshell membranes (P = 0.0014), and penetration of TNM into albumen (P = 0.0019). Storage time significantly affected egg weight and all parameters of eggshell quality except the eggshell strength and index. It also significantly affected count of Enterococcus (ENT) on eggshell, TNM in eggshell membranes and TNM in albumen. Storage temperature significantly influenced egg weight (P = 0.0001) and all parameters but eggshell thickness and surface. Regarding the microbial contamination, storage temperature significantly affected a count of ENT on shell, TNM in shell membranes, and TNM in albumen. Concerning significant interactions, the interaction among genotype and storage time was found significant (P = 0.0148). Fresh and 28-day-old commercial hybrid eggs were the most contaminated, whereas guinea fowl eggs (fresh and 14 d old) and Leghorn hen eggs (fresh, 14, 28 d old) had the lowest level of contamination by EC. When looking for an alternative to laying hens, guinea fowls should be taken into consideration due to their higher resistance to diseases, ability of adaptation to different environmental conditions, and especially in terms of eggshell quality and therefore egg safety.

Determination of selected biochemical parameters in blood serum and egg quality of Czech and Slovak native hens depending on the housing system and hen age.

The objective of this study was to determine and evaluate the impact of the age and housing system on blood indicators (triacylglycerides, total cholesterol, aspartate aminotransferase, total proteins, albumin, glucose) and physical egg quality parameters (egg weight, shape index and surface area, eggshell proportion, thickness, strength, and color, albumen proportion and index, Haugh units, yolk proportion, index and yolk-to-albumen ratio) in selected native breeds of the Czech Republic (the Czech Golden Spotted hens) and Slovakia (the Oravka hens). Furthermore, the concentration of cholesterol in the yolk was determined. A total of 132 animals were used. There were 60 eggs collected from each breed at each monitored period for the evaluation of egg quality. Blood samples were taken by puncture of a wing vein. The assessments were made when the hens were of 34, 42, and 50 weeks old. Enriched cages and floor pens with litter were used as housing systems. The effects of breed, housing system, and age were observed. Furthermore, interactions among these factors were calculated. The significant effect of housing system was found in total cholesterol (P = 0.098) and aspartate aminotransferase (P = 0.0343) and the significant effect of age in total protein (P = 0.0392). The significant effect of breed (P = 0.0199), housing system (P = 0.0001), and age (P = 0.0001) was found in concentration of cholesterol in the yolk. Regarding the egg quality, the significant effect of breed (P = 0.0001) was found in eggshell color, albumen index and Haugh units, whereas the significant effect of housing system was found in egg weight (P = 0.0002), egg surface area (P = 0.0003), eggshell proportion (P = 0.0460), thickness (P = 0.0216), strength (P = 0.0049), and color (P = 0.0009). The significant effect of age was determined in all parameters except for the eggshell proportion and strength. The results represent an interesting comparison of changes in biochemical blood and egg quality parameters. It is necessary to further evaluate these indicators, especially in other genetic resources of hens, where the data are often nonexisting.

Distances, luminosities, and temperatures of the coldest known substellar objects.

The coolest known brown dwarfs are our best analogs to extrasolar gas-giant planets. The prolific detections of such cold substellar objects in the past 2 years have spurred intensive follow-up, but the lack of accurate distances is a key gap in our understanding. We present a large sample of precise distances based on homogeneous mid-infrared astrometry that robustly establishes absolute fluxes, luminosities, and temperatures. The coolest brown dwarfs have temperatures of 400 to 450 kelvin and masses almost equal to 5 to 20 times that of Jupiter, showing they bridge the gap between hotter brown dwarfs and gas-giant planets. At these extremes, spectral energy distributions no longer follow a simple correspondence with temperature, suggesting an increasing role of other physical parameters, such as surface gravity, vertical mixing, clouds, and metallicity.