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1.
Genome ; 65(11): 563-572, 2022 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-36044774

RESUMO

The genus Ctenomys has been widely used in karyotype evolution studies due to the variation in their diploid numbers. Ctenomys minutus is characterized by intraspecific variation in diploid number (2n = 42, 46, 48, and 50), which makes it an interesting model to investigate genomic rearrangements mechanisms that could lead to different cytotypes in this species. Thereupon, it has been already shown that DNA methylation may participate in chromosome structure. Therefore, we aimed to investigate whether telomeres and global DNA methylation had a role in the genome rearrangements that led to this variation in C. minutus. We also realized an analysis for the presence of intrachromosomal telomeric repeats (ITRs) by fluorescence in situ hybridization. Our study demonstrated that neither telomere length nor DNA methylation had significant differences among the cytotypes. However, if only females were considered, there were significant differences for telomere length and methylation. Young individuals, regardless of their cytotypes, had the most methylated DNA. Regarding the ITRs, we found a signal on chromosome 1 in 2n = 50b. No evidence was found that telomere length or methylation could have influenced chromosomal rearrangements, although new cytotypes seem to have emerged within the distribution of parental cytotypes by the accumulation of different chromosomal rearrangements.


Assuntos
Roedores , Telômero , Humanos , Animais , Hibridização in Situ Fluorescente , Metilação , Cariótipo , Cariotipagem , Roedores/genética , Telômero/genética
2.
Rev Sci Instrum ; 92(5): 053701, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-34243353

RESUMO

The detection of bulk micro-defects in Czochralski-grown silicon (Si) 〈100〉 wafers has significant importance in wafer quality control. Light Scattering Tomography (LST) is an industry standard technique for this purpose. This optical non-contact metrology requires destructive sample preparation: Samples have to be cleaved into half. One particular feature of the method is a dark field detection arrangement, which is achieved by separating the light detection part (microscope unit) from the illumination. Illumination is applied to the front surface of the sample, and the light scattered off of the defects is collected via the cleaved surface. The technique requires the perpendicularity of the cleaved surface to the front surface, which is fulfilled for Si(100) wafers. However, the nominally cleaved surface for Si(111) wafers is not perpendicular to the front surface but has an angle of 70.5°. This significant difference in cleavage results in the fact that Si(111) wafers cannot be measured by standard LST systems. Fortunately, the standard LST system can be modified by tilting the detection part under a proper angle allowing the measurements of Si(111) samples. In this article, we present this new technique in detail, showing the design and measurement capability of the new system. The measurement results are validated by a direct comparison to standard LST measurements on the same samples after proper sample preparation.

3.
Rev Sci Instrum ; 92(4): 043701, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-34243430

RESUMO

The detection of oxygen precipitates, voids, and other defects is critical for semiconductor wafer makers. One of the industry standard techniques for detecting these Bulk Micro-Defects (BMDs) is Semilab's Light Scattering Tomograph (LST) system. In this measurement, unpatterned wafers are nominally cleaved in half. Illumination is applied to the front surface of the sample, and the light scattered off of the defects is collected via the cleaved surface. This technique had been limited to the measurement of unpatterned wafers, but device makers show significant interest in measuring BMD distributions on patterned wafers using scattering-based techniques. A pattern on the surface of the wafer can cause significant scattering, making the standard LST technique unsuitable for this task. We present a solution for patterned wafer BMD measurements by an addition of a low-angle illumination unit to the standard LST system. This new illumination unit focuses the light into the bulk of the wafer via the cleaved surface, which enables measurement on patterned samples. The new system is called "light scattering tomograph enhanced by low-angle illumination." Excellent correlation was found between the detected defect densities obtained by the low-angle and the standard LST illumination mode.

4.
BMC Microbiol ; 16(1): 158, 2016 07 20.
Artigo em Inglês | MEDLINE | ID: mdl-27439312

RESUMO

BACKGROUND: Pet is a toxin from the family of Serine Protease Autotransporters of Enterobacteriaceae which was initially identified in Enteroaggregative Escherichia coli strains. This protease exhibits enterotoxin properties, damages the cell cytoskeleton and induces intestinal epithelium alterations, which are associated with a severe inflammatory process. An in-vitro study was conducted to evaluate the effect of Pet on the migration of human peripheral blood monocytes-derived macrophages and its participation in the activation of the early inflammatory response and cytokine expression. RESULTS: In the macrophage migration activation assay, Pet produced a similar effect to that induced by opsonized zymosan (ZAS). Regarding the cytokine expression, an increase of IL-8, TNF-α (pro-inflammatory) and IL-10 (anti-inflammatory) was identified. In addition to the above results, the nuclear translocation of NF-kB pp65 was also identified. These events are probably related to the inflammatory response identified in the histological examination of intestine rat samples inoculated with Pet during a ligated loop assay. CONCLUSION: The results showed that Pet participates as an immunostimulant molecule for macrophages, which activates both their mobility and cytokine expression. These observations suggest that the toxin participates in the inflammatory process that is observed during the host infection by EAEC Pet producing.


Assuntos
Toxinas Bacterianas/química , Toxinas Bacterianas/toxicidade , Enterotoxinas/química , Enterotoxinas/toxicidade , Proteínas de Escherichia coli/química , Proteínas de Escherichia coli/toxicidade , Escherichia coli/enzimologia , Ativação de Macrófagos/efeitos dos fármacos , Serina Endopeptidases/química , Serina Endopeptidases/toxicidade , Animais , Toxinas Bacterianas/metabolismo , Linhagem Celular , Quimiotaxia/efeitos dos fármacos , Citocinas/biossíntese , Citocinas/imunologia , Citoesqueleto/metabolismo , Enterotoxinas/metabolismo , Proteínas de Escherichia coli/metabolismo , Humanos , Imunidade Inata , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/patologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Macrófagos/metabolismo , Fagocitose , Ratos , Ratos Sprague-Dawley , Serina Endopeptidases/metabolismo , Zimosan
5.
Eur J Endocrinol ; 155(1): 137-42, 2006 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16793960

RESUMO

OBJECTIVE: Only a few studies have investigated variations of different markers for inflammatory processes during the physiological menstrual cycle. The results are conflicting, particularly concerning the correlation between the marker leptin and steroid hormones. The aim of the study was to investigate the inflammatory markers C-reactive protein (CRP) and leptin in the serum of healthy, normally ovulating women and to correlate these with each other and with the hormones of the gonadal axis. A cycle-dependence of the markers studied would imply an exact timing of the blood sampling for clinical needs. DESIGN: Observational study investigating the two inflammatory markers CRP and leptin in relation to the hormonal pattern of the gonadal axis during the normal cycle. METHODS: Ovulatory cycles of 36 healthy, young, normo-androgenic women, having a normal body mass index were evaluated. Serum concentrations of leptin and CRP, as well as of follicle-stimulating hormone, luteinising hormone, 17beta-oestradiol, progesterone, prolactin (PRL) and free testosterone were measured every 1-2 days during one full cycle. RESULTS: Serum levels of leptin and CRP behaved differently during ovulatory cycles, with higher concentrations for leptin only during certain phases. Significant correlations were found in the follicular phase between leptin and PRL and leptin and free testosterone. CONCLUSIONS: Leptin levels change during the menstrual cycle. Leptin levels are more stable on cycle days 1-5 than later in the cycle. For precise cycle-independent measurements, these fluctuations have to be taken into account. There is no similar cyclic pattern for CRP.


Assuntos
Proteína C-Reativa/metabolismo , Leptina/sangue , Ciclo Menstrual/fisiologia , Adulto , Índice de Massa Corporal , Estrogênios/sangue , Feminino , Hormônio Foliculoestimulante/sangue , Gonadotropinas/sangue , Humanos , Ovulação , Prolactina/sangue , Valores de Referência , Esteroides/sangue
6.
Parasitol Res ; 98(3): 232-6, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16341883

RESUMO

Amebiasis and rabies are public health problems, and they have in common a poor inflammatory effect in the target organs that they affect. In the GenBank, it was found that the anti-inflammatory peptide monocyte locomotion inhibitory factor (MLIF) produced by Entamoeba histolytica homologates 80%, with a fragment of the N protein of the rabies virus. We speculated if the N protein could contribute to the scant inflammatory reaction produced by rabies virus in central nervous system. The N protein was obtained and studied in vitro and in vivo. The N protein, as MLIF, inhibited the respiratory burst in human mononuclear phagocytes (43%, p<0.05), but in contrast to MLIF, it increased chemotaxis and it did not significantly inhibit delayed hypersensitivity skin reaction to 1-chloro-2-4-dinitrobenzene in guinea pigs. Therefore, the full peptide sequence has to be present or it has to be cleaved-free from the large recombinant N protein molecule (55 kDa) to become active.


Assuntos
Entamoeba histolytica/metabolismo , Inflamação/metabolismo , Proteínas do Nucleocapsídeo/farmacologia , Oligopeptídeos/metabolismo , Proteínas de Protozoários/metabolismo , Animais , Linhagem Celular , Movimento Celular , Fatores Quimiotáticos , Entamoeba histolytica/efeitos dos fármacos , Cobaias , Hipersensibilidade Tardia , Mariposas/citologia , Proteínas do Nucleocapsídeo/química , Proteínas do Nucleocapsídeo/metabolismo , Oligopeptídeos/química , Explosão Respiratória
7.
Epidemiol Infect ; 133(3): 517-22, 2005 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-15966107

RESUMO

Salivary excretion of rabies virus was evaluated in 14 adult vampire bats (Desmodus rotundus) intramuscularly injected with a large dose (10(6) MICLD50) of vampire rabies virus variant CASS88. Saliva samples were obtained from surviving bats every other day for 30 days, then weekly for 2 months, and finally 1 and 2 years later. Rabies virus was isolated in murine neuroblastoma cells and in randomly selected cases by PCR. Rabies virus was not detected in the saliva of any of the 11 animals that succumbed (somewhat early) to rabies challenge, nor in the control bats. In contrast, virus was detected early, and only once (days 6, 6 and 21) in each of the three animals that survived rabies challenge and remained healthy for at least 2 years after challenge. At that time even vigorous dexamethasone and cyclosporine administration failed to provoke further viral excretion.


Assuntos
Quirópteros/virologia , Vírus da Raiva/patogenicidade , Raiva/veterinária , Saliva/virologia , Animais , DNA Viral/análise , Feminino , Masculino , Reação em Cadeia da Polimerase , Raiva/virologia
8.
Hum Reprod ; 20(5): 1266-71, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15746199

RESUMO

BACKGROUND: There are only a few studies that have investigated inflammatory processes during ovarian hyperstimulation, with contradictory results especially concerning outcome. The aim of the study was to investigate the inflammatory markers C-reactive protein and leptin in serum and follicular fluid and to correlate these with the outcome. METHODS: One hundred and sixty-two gonadotrophin stimulated cycles were evaluated. Serum concentrations of leptin and C-reactive protein were measured at the initiation of stimulation, on the day of hCG administration or the day before, and on the day of oocyte retrieval. They were also determined in the follicular fluid. RESULTS: Serum leptin and C-reactive protein levels increased significantly during stimulation until the day of oocyte pick up, but following different patterns. After stimulation, they correlated with each other in serum and follicular fluid, but not with estradiol or progesterone concentration, embryo quality, or the pregnancy rate. CONCLUSIONS: Leptin and C-reactive protein levels change significantly during assisted reproductive treatment. In contrast to estradiol they are, however, not a marker of success.


Assuntos
Proteína C-Reativa/metabolismo , Líquido Folicular/metabolismo , Leptina/metabolismo , Técnicas de Reprodução Assistida , Adulto , Biomarcadores , Estradiol/sangue , Feminino , Humanos , Leptina/sangue , Idade Materna , Oócitos/fisiologia , Indução da Ovulação/métodos , Gravidez , Injeções de Esperma Intracitoplásmicas/métodos , Esteroides/sangue , Esteroides/metabolismo , Fatores de Tempo , Resultado do Tratamento
9.
Parasitol Res ; 90(4): 264-7, 2003 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12884017

RESUMO

The monocyte locomotion inhibitory factor, an anti-inflammatory pentapeptide produced by Entamoeba histolytica, inhibits the in vitro production of nitric oxide induced by cytokines (INF-gamma, TNF-alpha) or PMA in human leukocytes. This can be added to the other previously reported functional effects of this factor, such as the inhibition of monocyte locomotion and the synthesis of reactive oxygen intermediates in both monocytes and neutrophils. The decreased nitric oxide production may interfere with the killing of amebas by neutrophils in the early invasive stages of amebiasis, when oxidative mechanisms are used [reactive oxygen and nitrogen intermediates either individually or synergistically via peroxynitrite (ONOO(-))], and in the advanced stages, when both non-oxidative and oxidative (including nitric oxide) mechanisms are employed by macrophages. Diminished nitric oxide production by leukocytes may also contribute to the paucity of late inflammatory components in amebic abscess of the liver and other amebic lesions.


Assuntos
Entamoeba histolytica/patogenicidade , Leucócitos/efeitos dos fármacos , Óxido Nítrico/biossíntese , Oligopeptídeos/farmacologia , Animais , Quimiotaxia de Leucócito , Entamoeba histolytica/metabolismo , Entamebíase/imunologia , Entamebíase/parasitologia , Interações Hospedeiro-Parasita , Humanos , Leucócitos/parasitologia , Leucócitos/fisiologia , Neutrófilos/parasitologia , Oligopeptídeos/metabolismo , Fagócitos/parasitologia , Células U937
11.
Water Sci Technol ; 44(5): 287-93, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11695472

RESUMO

Diglyme (CAS No. 111-96-6), a biorefractive ether with teratogenic properties is of considerable importance as a solvent in the synthetic chemical industry. Results of lab-scale investigations into the optimal conditions for the oxidative mineralisation of 0.05 to 1 g/L of diglyme in synthetic process waters by hydrogen peroxide and ozone based advanced oxidation processes are presented. Fenton, photo-assisted Fenton and UV/H2O2 oxidation processes show acceptable TOC removals. At 50 degrees C the initial TOC removal rates varies between 0.07 and 6g TOC/L*h. The rates increase with the initial diglyme and hydrogen peroxide concentration as well as with the UV irradiation energy intensity. For example at a 1 g/l diglyme concentration a stoichiometric H2O2 addition resulted in TOC degradation of 60% to 70% after 30 minutes under the investigated conditions. Treatment with ozone/H2O2 at a pH of 8 and 25 degrees C required at least 100 minutes to achieve comparable mineralisation results. Biodegradability can be reached in far shorter times. Ozone on its own cannot be recommended for diglyme treatment. If complete mineralisation of diglyme is the objective, the UV/H2O2 oxidation process should be favoured. The ozone/H2O2 process might offer a viable alternative in cases where the oxidation step is followed by biological wastewater treatment, so that biodegradability is aimed at.


Assuntos
Etilenoglicóis/química , Éteres Metílicos/química , Solventes/química , Eliminação de Resíduos Líquidos/métodos , Purificação da Água/métodos , Biodegradação Ambiental , Indústria Química , Desenho de Equipamento , Peróxido de Hidrogênio/química , Ferro , Oxidantes/química , Oxidantes Fotoquímicos/química , Oxirredução , Ozônio/química , Raios Ultravioleta , Poluentes Químicos da Água
12.
Clin Chem Lab Med ; 39(6): 527-30, 2001 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11506466

RESUMO

The carbohydrate antigen 19-9 (CA 19-9), a determinant (sialylated lacto-N-fucopentaose 119) of a circulating oligosaccharide antigen, is a frequently used tumor marker. Echinococcus spp. infects humans throughout the world and may be able to synthesize closely related molecules which could interfere with the measurement and interpretation of CA 19-9 concentration. The main objective of the present study was to determine the range of CA 19-9 levels in the sera of patients infected by E. granulosus (cystic hydatide disease; CYSHD) or E. multilocularis (alveolar hydatide disease; ALVHD). Serum samples were collected from patients (aged 10-85 years) over a period of 5 years: from 19 patients with CYSHD and from 20 patients with ALVHD. Infection was confirmed by positive Echinococcus serology and clinical evidence provided by imaging and/or histopathological findings. CA 19-9 was detectable in 13 patients with CYSHD (13.5 +/- 8.5 kU/l) and 13 patients with ALVHD (30.0 +/- 21 kU/l; p < 0.05). Thus ALVHD patients exhibited a significantly higher plasma level of CA 19-9 than CYSHD patients. The serum level of CA 19-9 assessed with an increased cut-off value (> 22 kU/l) was elevated in nine (45%) of 20 ALVHD patients compared to two (11%) of 19 CYSHD patients (p < 0.05). Sera from patients with Echinococcus multilocularis infection contain substances which cross-react with CA 19-9. These substances originate either from the parasite or are synthesized by the host in response to the infection, and possibly bear the Lewis-a antigen or closely related structures which are recognized by anti-CA 19-9 antibodies. Our findings are relevant to the investigation of patients presenting with cystic lesions for which the differential diagnosis includes an infectious or neoplastic origin.


Assuntos
Antígeno CA-19-9/sangue , Equinococose Hepática/imunologia , Equinococose/imunologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Antígenos de Helmintos/sangue , Criança , Reações Cruzadas , Diagnóstico Diferencial , Equinococose/sangue , Equinococose/diagnóstico , Equinococose Hepática/sangue , Equinococose Hepática/diagnóstico , Echinococcus/imunologia , Feminino , Humanos , Antígenos do Grupo Sanguíneo de Lewis/sangue , Hepatopatias/sangue , Hepatopatias/diagnóstico , Hepatopatias/imunologia , Masculino , Pessoa de Meia-Idade
13.
Invest Ophthalmol Vis Sci ; 42(7): 1653-9, 2001 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-11381074

RESUMO

PURPOSE: Evidence has accumulated that excessive light exposure may promote age-related and inherited retinal degeneration, in which photoreceptor death by apoptosis leads to loss of vision. In the current study, the effect of elevated corticosteroid levels on light-induced apoptosis of photoreceptors was determined. METHODS: Photoreceptor apoptosis was induced in retinas of BALB/c mice by exposure to diffuse white light. High levels of corticosteroids were induced, either endogenously (fasting-mediated stress) or by a single intraperitoneal injection of dexamethasone (DEX). Photoreceptor damage was assessed morphologically and by electroretinography. Glucocorticoid receptor (GR) and activator protein (AP)-1 activities were shown by Western blot analysis and electrophoretic mobility shift assay (EMSA) of retinal nuclear extracts. RESULTS: Fasting and injection of DEX led to an activation of GR in the retina, as judged by its translocation to the nucleus of retinal cells. On induction of GR activity before light exposure, AP-1 activity, normally induced by damaging doses of light, remained at basal levels. Both treatments completely prevented photoreceptor apoptosis and preserved retinal function. CONCLUSIONS: Activity of the transcription factor AP-1 is associated with light-induced apoptosis. In the current study, pharmacologic suppression of AP-1 activity protected against light damage. Inhibition of AP-1 activity may have occurred by the protein-protein interaction of GR and AP-1.


Assuntos
Apoptose , Luz/efeitos adversos , Células Fotorreceptoras de Vertebrados/efeitos da radiação , Lesões Experimentais por Radiação/prevenção & controle , Receptores de Glucocorticoides/metabolismo , Degeneração Retiniana/prevenção & controle , Animais , Apoptose/efeitos da radiação , Western Blotting , Corticosterona/sangue , Dexametasona/farmacologia , Eletrorretinografia , Privação de Alimentos , Glucocorticoides/farmacologia , Injeções Intraperitoneais , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Células Fotorreceptoras de Vertebrados/efeitos dos fármacos , Células Fotorreceptoras de Vertebrados/metabolismo , Lesões Experimentais por Radiação/etiologia , Lesões Experimentais por Radiação/metabolismo , Lesões Experimentais por Radiação/patologia , Degeneração Retiniana/etiologia , Degeneração Retiniana/metabolismo , Degeneração Retiniana/patologia , Fator de Transcrição AP-1/antagonistas & inibidores , Fator de Transcrição AP-1/metabolismo
14.
Arch Orthop Trauma Surg ; 121(4): 223-6, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11317685

RESUMO

Metallic orthopaedics implants are composed of elements that are known to be skin sensitizers in the general population. In this study, we analyzed the cells of perivascular infiltration in the tissue adjacent to titanium (n = 23) and steel (n = 8) implants after explantation of the metals by immunohistochemical methods. The following panel of monoclonal antibodies were used as parameters: CD 1a (Langerhans cells), CD 4 (T-helper cells), CD 8 (T-suppressor cells), CD 11c (monocytes and macrophages), CD 45 RO (memory cells), CD 45 RA (naive cells), eosinophil cationic proteins (ECP), neutrophil elastase, and HLA-DR. The number of perivascular total cells did not differ significantly. All cells were identified in both metal subgroups, but a statistical difference was not seen in the above-mentioned parameters. We conclude that sensitization to metals is possible in the tissue adjacent to steel and titanium implants, because all cells which play an important role in allergic delayed-type hypersensitivity (type IV) reactions are present. This phenomenon may be called a 'pre-sensitization' phase, because no sensitization or allergic reactions were seen in our cases. Second, in the present study, a statistical difference was not seen in the number of infiltrate cells in the tissue adjacent to steel compared with titanium implants.


Assuntos
Antígenos CD/análise , Reação a Corpo Estranho/imunologia , Fixação Interna de Fraturas/instrumentação , Hipersensibilidade Tardia/imunologia , Prótese Articular , Leucócitos/imunologia , Ribonucleases , Aço Inoxidável , Titânio , Adulto , Idoso , Proteínas Sanguíneas/análise , Remoção de Dispositivo , Proteínas Granulares de Eosinófilos , Feminino , Reação a Corpo Estranho/patologia , Humanos , Hipersensibilidade Tardia/patologia , Contagem de Leucócitos , Elastase de Leucócito/análise , Leucócitos/patologia , Masculino , Pessoa de Meia-Idade , Testes do Emplastro
15.
Mol Biochem Parasitol ; 112(2): 201-9, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11223127

RESUMO

The monocyte locomotion inhibitory factor (MLIF), a heat-stable oligopeptide found in the supernatant fluid of Entamoeba histolytica axenic cultures was isolated by ultra-filtration, gel-sieve chromatography and high powered liquid chromatography (HPLC), and its primary structure (Met-Gln-Cys-Asn-Ser) established by Edman sequencing and mass-spectrometry (MS). A synthetic peptide had the same selective anti-inflammatory features as the native material in comparable concentrations: in vitro inhibition of the locomotion in human peripheral blood monocytes, and of the respiratory burst in the same cells and in human neutrophil polymorphonuclear leucocytes; and in vivo depression of delayed hypersensitivity skin reactions to dinitrochlorobenzene in guinea pigs. This oligopeptide is apparently synthesized by the ameba as suggested by [(35)S]-Cys and Met incorporation, probably as part of a larger molecule, from which it is cleaved by proteolysis. The full sequence was not found in the 431 available E. histolytica protein sequences. The factor may contribute to the unexpected paucity of the late inflammatory reaction found in advanced invasive amebiasis and, perhaps in consequence, to the regeneration without scarring (restitutio ad integrum) of the affected organs that is observed following successful treatment of this disease


Assuntos
Anti-Inflamatórios/imunologia , Anti-Inflamatórios/isolamento & purificação , Entamoeba histolytica/química , Entamoeba histolytica/imunologia , Oligopeptídeos/imunologia , Oligopeptídeos/isolamento & purificação , Adulto , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/metabolismo , Quimiotaxia de Leucócito , Cromatografia Líquida de Alta Pressão , Dinitroclorobenzeno/imunologia , Entamoeba histolytica/metabolismo , Entamebíase/imunologia , Entamebíase/parasitologia , Cobaias , Humanos , Hipersensibilidade Tardia/imunologia , Monócitos/imunologia , Neutrófilos/imunologia , Oligopeptídeos/biossíntese , Oligopeptídeos/química , Proteínas de Protozoários/biossíntese , Proteínas de Protozoários/química , Proteínas de Protozoários/imunologia , Proteínas de Protozoários/isolamento & purificação , Explosão Respiratória , Análise de Sequência de Proteína , Testes Cutâneos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
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