Biomass removal promotes plant diversity after short-term de-intensification of managed grasslands.

Land-use intensification is one of the main drivers threatening biodiversity in managed grasslands. Despite multiple studies investigating the effect of different land-use components in driving changes in plant biodiversity, their effects are usually studied in isolation. Here, we establish a full factorial design crossing fertilization with a combined treatment of biomass removal, on 16 managed grasslands spanning a gradient in land-use intensity, across three regions in Germany. Specifically, we investigate the interactive effects of different land-use components on plant composition and diversity using structural equation modelling. We hypothesize that fertilization and biomass removal alter plant biodiversity, directly and indirectly, mediated through changes in light availability. We found that, direct and indirect effects of biomass removal on plant biodiversity were larger than effects of fertilization, yet significantly differed between season. Furthermore, we found that indirect effects of biomass removal on plant biodiversity were mediated through changes in light availability, but also by changes in soil moisture. Our analysis thus supports previous findings, that soil moisture may operate as an alternative indirect mechanism by which biomass removal may affect plant biodiversity. Most importantly, our findings highlight that in the short-term biomass removal can partly compensate the negative effects of fertilization on plant biodiversity in managed grasslands. By studying the interactive nature of different land-use drivers we advance our understanding of the complex mechanisms controlling plant biodiversity in managed grasslands, which ultimately may help to maintain higher levels of biodiversity in grassland ecosystems.

Vegetation characteristics control local sediment and nutrient retention on but not underneath vegetation in floodplain meadows.

Sediment and nutrient retention are essential ecosystem functions that floodplains provide and that improve river water quality. During floods, the floodplain vegetation retains sediment, which settles on plant surfaces and the soil underneath plants. Both sedimentation processes require that flow velocity is reduced, which may be caused by the topographic features and the vegetation structure of the floodplain. However, the relative importance of these two drivers and their key components have rarely been both quantified. In addition to topographic factors, we expect vegetation height and density, mean leaf size and pubescence, as well as species diversity of the floodplain vegetation to increase the floodplain's capacity for sedimentation. To test this, we measured sediment and nutrients (carbon, nitrogen and phosphorus) both on the vegetation itself and on sediment traps underneath the vegetation after a flood at 24 sites along the River Mulde (Germany). Additionally, we measured biotic and topographic predictor variables. Sedimentation on the vegetation surface was positively driven by plant biomass and the height variation of the vegetation, and decreased with the hydrological distance (total R2 = 0.56). Sedimentation underneath the vegetation was not driven by any vegetation characteristics but decreased with hydrological distance (total R2 = 0.42). Carbon, nitrogen and phosphorus content in the sediment on the traps increased with the total amount of sediment (total R2 = 0.64, 0.62 and 0.84, respectively), while C, N and P on the vegetation additionally increased with hydrological distance (total R2 = 0.80, 0.79 and 0.92, respectively). This offers the potential to promote sediment and especially nutrient retention via vegetation management, such as adapted mowing. The pronounced signal of the hydrological distance to the river emphasises the importance of a laterally connected floodplain with abandoned meanders and morphological depressions. Our study improves our understanding of the locations where floodplain management has its most significant impact on sediment and nutrient retention to increase water purification processes.

Plant structural diversity alters sediment retention on and underneath herbaceous vegetation in a flume experiment.

Sediment retention is a key ecosystem function provided by floodplains to filter sediments and nutrients from the river water during floods. Floodplain vegetation is an important driver of fine sediment retention. We aim to understand which structural properties of the vegetation are most important for capturing sediments. In a hydraulic flume experiment, we investigated this by disentangling sedimentation on and underneath 96 vegetation patches (40 cm x 60 cm). We planted two grass and two herb species in each patch and conducted a full-factorial manipulation of 1) vegetation density, 2) vegetation height, 3) structural diversity (small-tall vs tall-tall species combinations) and 4) leaf pubescence (based on trait information). We inundated the vegetation patches for 21 h in a flume with silt- and clay-rich water and subsequently measured the amount of accumulated sediment on the vegetation and on a fleece as ground underneath it. We quantified the sediment by washing it off the biomass and off the fleece, drying the sediment and weighting it. Our results showed that all manipulated vegetation properties combined (vegetation density and height, and the interaction of structural diversity and leaf pubescence) explained sedimentation on the vegetation (total R2 = 0.34). The sedimentation underneath the vegetation was explained by the structural diversity and the leaf pubescence (total R2 = 0.11). We further found that vegetation biomass positively affected the sedimentation on and underneath the vegetation. These findings are crucial for floodplain management strategies with the aim to increase sediment retention. Based on our findings, we can identify management strategies and target plant communities that are able to maximize a floodplain's ability to capture sediments.

Leaf area and pubescence drive sedimentation on leaf surfaces during flooding.

Worldwide, stream water is increasingly loaded with sediments and nutrients, due to processes such as accelerated soil erosion and overfertilization caused by agricultural intensification. This leads to increases in eutrophication and silting up of bottom sediments. Floodplains can play an important role in mitigating these problems, by removing sediment from rivers via water filtration and retention. Fine sediment is accumulated on the soil in between plants as well as on plant surfaces. However, it is still poorly understood how plant species facilitate leaf surface sedimentation via their leaf traits. In a flume experiment, we investigated to what extent the leaf traits (area, length, perimeter, pinnation, pubescence, surface roughness, flexibility and wettability) influence leaf surface sedimentation. We exposed leaves of 30 plant species to an artificial flood, and measured the fine sediment load the leaves captured after 24 h. Our results show that leaf traits overall explain 65% of the variation of fine sedimentation on leaves. Especially adaxial pubescence and leaf area strongly drove sedimentation. Hairy leaves accumulate more sediment per leaf area, presumably, because hairs create a buffer zone of reduced flow velocity which enhances sedimentation between the hairs. Additionally, for leaves with no or few hairs, sedimentation decreased with increasing leaf area, because most likely the more turbulent boundary layer of larger leaves allows less sediment to settle. Our results provide a first understanding of how plants can be selected based on their leaf traits for maximizing the sediment retention on floodplains, thereby providing a key ecosystem service.